scholarly journals Biostimulative effect of amino acids and green algae extract on capsaicinoid and other metabolite contents in fruits of Capsicum spp.

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Tilen Zamljen ◽  
Metka Hudina ◽  
Robert Veberič ◽  
Ana Slatnar
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Green Algae ◽  
Acid Treatment ◽  
Sugar Content ◽  
Control Treatment ◽  
Hot Pepper ◽  
Large Variability ◽  
Total Sugar Content ◽  
Capsicum Spp

Abstract Background Biostimulants, such as algae extracts or amino acids, are becoming more common in agriculture because the mentality is to make plants more resistant or tolerant to negative environmental factors, rather than using synthetic chemicals (herbicides, insecticides and fungicides), whose use is decreasing year by year, forcing farmers and companies to develop new environmentally friendly products. Results In a field experiment, green algae and amino acids were tested as biostimulants on three hot pepper cultivars. A large variability was observed between the effects of the two biostimulants on the cultivars. Green algae-treated ‘Somborka’ and ‘Habanero Red Caribbean’ cultivars had 10% and 5% higher dry matter in seeds compared to control treatment. Total sugar content was negatively affected by green algae extract and amino acids in pericarp in cultivars ‘Chili AS-Red’ and ‘Habanero Red Caribbean’. Total organic acids content was positively affected by amino acid treatment in ‘Habanero Red Caribbean’ pericarp and placenta, with an increase of 5.5 g and 2.1 g/100 g DW, respectively. In terms of total phenolics, all three cultivars were positively affected by amino acid treatment, but not in each fruit part. In terms of capsaicinoid content, the greatest effect of the two stimulants was on ‘Somborka’, which varied from four (pericarp, seed) to 16 (placenta) times compared to the control. Amino acid extract decreased ‘Habanero Red Caribbean’ capsaicinoid content in placenta by about 40%. Conclusion Amino acids were found to be superior to algal extract, although the effect of both was mostly cultivar specific. For a wider use of biostimulants, it should first be tested whether they are suitable for the cultivar in order to increase and optimize the results. Graphic abstract

Root uptake and xylem transport of cadmium in wheat and triticale as affected by exogenous amino acids

2017 ◽  
Vol 68 (5) ◽  
pp. 415 ◽  
Author(s):  
K. Khodamoradi ◽  
A. H. Khoshgoftarmanesh ◽  
S. A. M. Mirmohammady Maibody
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Bread Wheat ◽  
Nutrient Solution ◽  
Metal Ion ◽  
Xylem Sap ◽  
Root Uptake ◽  
Plant Roots ◽  
Control Treatment ◽  
Long Distance

Organic acids exuded from plant roots significantly modify uptake and long-distance translocation of metals. Little is known about the effect of amino acids on metal ion uptake by plant roots. The present study investigated the effects of exogenous amino acids (histidine and glycine) in a nutrient solution on root uptake and xylem sap transport of cadmium (Cd) in triticale (× Triticosecale cv. Elinor) and bread wheat (Triticum aestivum L. cv. Back Cross Rushan). Plant seedlings were grown in a Cd-free modified Hoagland nutrient solution to which 1 µm Cd was added with either 50 µm histidine or 50 µm glycine or without amino acids at 4 weeks after germination. A control treatment consisted of a nutrient solution free of Cd and amino acids. In bread wheat, addition of histidine to the Cd-containing nutrient solution resulted in a higher operationally defined symplastic Cd fraction but a lower apoplastic one in the roots. In triticale, addition of either amino acid decreased the symplastic Cd fraction but increased the apoplastic one. Addition of histidine to the nutrient solution increased Cd concentration in wheat xylem sap but had no significant effect on Cd concentration in triticale xylem sap. Compared with the Cd-only treatment, the glycine-containing treatment led to significantly reduced Cd concentrations in xylem sap of both plant species. Wheat plants supplied with histidine and Cd accumulated greater amounts of Cd in their shoots than those supplied with Cd alone. Glycine had no significant effects on the Cd content of wheat shoots but decreased it in triticale shoots. Results indicate that the effects of amino acids on plant root uptake and xylem sap translocation of Cd depend on the type of amino acid supplemented. This finding is of great importance for selecting and/or breeding cultivars with Cd-toxicity tolerance.

scholarly journals The Effect of Amino Acids and The Date of Planting on Yield and Some Yield Components of Three Maize Varieties

2021 ◽  
Vol 904 (1) ◽  
pp. 012066
Author(s):  
Y A Al-Janabi ◽  
N M Abood ◽  
M I Hamdan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Agricultural Research ◽  
Block Design ◽  
Control Treatment ◽  
Research Station ◽  
Spring Season ◽  
Planting Dates ◽  
Maize Varieties ◽  
Biological Yield

Abstract The experiment was carried out according to the randomized complete block design (R.C.B.D) and the arrangement of split-split plot with three replications, at the Field Crops Research Station of the Agricultural Research Department - Abu Ghraib during the spring season for the years 2019 and 2020. With the aim of studying the effect of amino acids, planting dates and their effect on some of the yield characteristics and its components for three varieties of maize in the spring season. Planting dates (March 15, March 25 and April 5) occupied the main plots, while amino acids and the control treatment (proline, arginine, and distilled water) occupied the sub-plot, whereas the varieties (Rabi, Baghdad 3 and Fajr 1) occupied the sub-sub-plot, and the most important results were summarized as follows:The two cultivars, Rabi and Fajr-1, achieved a significant increase in most of the yield characteristics and its components for the seasons. The addition of the amino acid proline also led to a significant increase in most of the studied traits (the number of grains in ear, weight of 500 grains, total grain and biological yield, and harvest index), planting dates also have a significant effect on most of the studied traits. The first date (15/3) recorded increase in the number of grains per ear, the weight of 500 grams, and the grain and biological yield. The plants of the Rabi variety when cultivated on March 15 th and sprayed with the amino acid proline achieved the highest average for the grain yield, which reached 7.48 ton. ha-1 for spring 2019.

scholarly journals INVESTIGATION AMINO ACID COMPOSITION OF DONKEY’S MILK OF THE KEGETY GORGE

InterConf ◽  
2021 ◽  
pp. 363-369
Author(s):  
Nadira Turganbaeva ◽  
Dinara Isakova
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Human Milk ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Sugar Content ◽  
Essential Amino Acids ◽  
Cow’S Milk ◽  
Donkey Milk ◽  
Cow's Milk

The objective of this paper was to study the amino acid composition of donkey milk inhabiting the Kegety gorge of the Chui region. As well as human milk, donkey and mare’s milk is albumin; the amount of casein is 35-45%, while cow’s milk is casein milk with casein content over 75%. The peculiarity of albumin milk is a higher biological and nutritional value, due to better amino acid balance, high sugar content and the ability to form small, delicate flakes when souring. In terms of its properties, albumin milk is closest to human milk and is the best substitute for it. The obtained results showed that the quantitative content of 7 essential amino acids out of 8, such as lysine, phenylalanine, leucine, isoleucine, methionine, valine, threonine is relatively high compared to other amino acids. Despite the absence of tryptophan in donkey milk, the percentage of 8 essential amino acids in donkey milk protein exceeds that in mare and cow’s milk.

scholarly journals Improving Storability of “Nanfeng” Mandarins by Treating with Postharvest Hot Water Dipping

2020 ◽  
Vol 2020 ◽  
pp. 1-12 ◽  
Author(s):  
İbrahim Kahramanoğlu ◽  
Chuying Chen ◽  
Yao Chen ◽  
Jinyin Chen ◽  
Zengyu Gan ◽  
...  
Keyword(s):  
Respiration Rate ◽  
Sugar Content ◽  
Storage Period ◽  
Hot Water ◽  
Control Treatment ◽  
Total Sugar Content ◽  
Titratable Acid ◽  
Fruit Decay ◽  
Mda Content ◽  
Hot Water Dipping

The current research aimed at studying the possibility of improving the postharvest storability of “Nanfeng” mandarins by hot water dipping (HWD) treatment. The research was conducted in two phases. Firstly, two different temperatures (50 and 55°C) were tested for three different dipping durations (2, 3, and 4 min) on the mandarin fruits, and the best combination was defined for the prevention of weight loss and fruit decay. Next, the optimal treatment (HWD at 50°C for 3 min) was used in further studies to test the effects on the postharvest fruit quality attributes. Regular measurements were performed to determine total soluble solid (TSS) content, titratable acid (TA) content, vitamin C (VC) content, total sugar content, respiration rate, malondialdehyde (MDA) content, and activities of superoxide dismutase (SOD) enzyme, polyphenoloxidase (PPO) enzyme, and peroxidase (POD) enzyme. According to the results obtained, HWD treatment was found to prevent the loss of TSS, TA, and VC contents during the storage period. The HWD-treated fruits were also found to have a lower respiration rate and MDA content as compared with control treatment. Furthermore, HWD treatment significantly enhanced the activities of SOD, POD, and PPO which are known to enhance tolerance to lipid peroxidation and are associated with the fruit protection from injuries and pathogens. Present results also suggest that the activation of the SOD and POD enzymes is highly related to the respiratory activities of the fresh produce. This suggests that the HWD can be used to improve the storability of “Nanfeng” mandarins by maintaining the postharvest physical and biochemical quality.

scholarly journals Effect of Organic Fertilization and Amino Acids on Growth, Chemical Composition and Capsaicin Content of Hot Pepper (Capsicum annum L var. Minimum) Plant

2020 ◽  
pp. 40-52
Author(s):  
K. A. Hammam ◽  
E. A. Eisa ◽  
A. A. Dewidar
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Chemical Composition ◽  
Vegetative Growth ◽  
Cattle Manure ◽  
Organic Fertilizers ◽  
Hot Pepper ◽  
Organic Fertilization ◽  
Full Dose ◽  
Capsicum Annum

This investigation was conducted in a private farm, Samannoud district, El-Gharbia Governorate, Egypt, during two successive seasons of 2017 and 2018 to study the effect of organic fertilizers and foliar spray of amino acid on vegetative growth, fruit yield, chemical composition and capsaicin content of hot pepper (Capsicum annum L var. Minimum) plant. The experiment included 13 treatments obtained from cattle manure at rates of 60 and 30 N-units per fed. combined with amino acid at 0, 100 and 200 ppm as well as chicken fertilizer at rates of 60 and 30 N-units per fed combined with amino acid at 0, 100 and 200 ppm, besides the treatment of NPK at recommended dose as a control. A complete randomized block design with three replicates was adopted. The obtained results showed that, the highest values from vegetative growth parameters,  fruit yield, chemicals composition and capsaicin content were obtained from plants treated with cattle manure full dose + 200 ppm amino acids , followed by plants treated with chicken manure full dose + 200 ppm amino acids compared with all treatments except plant height, the highest value was obtained from plants treated with NPK (control) in the two seasons. Amino acid had a great effect when added with organic fertilization, whether cattle manure or chicken fertilizer at a concentration of 100 or 200 ppm. Based on the previous results, it could be recommend that spraying amino acids (glycine and glutamine) at the rate of 200 ppm along with organic fertilizers at the rate of 60 nitrogen units per feddan get the highest fruits yield, Vitamin C and capsaicin of hot pepper.

scholarly journals Metabolic Variation among Fruits of Different Chili Cultivars (Capsicum spp.) Using HPLC/MS

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 101
Author(s):  
Tilen Zamljen ◽  
Aljaž Medič ◽  
Robert Veberič ◽  
Metka Hudina ◽  
Jerneja Jakopič ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Sugar Content ◽  
Dry Weight ◽  
Total Phenolic ◽  
Total Sugar Content ◽  
Capsicum Spp ◽  
Metabolic Variation ◽  
Organic Acid Content ◽  
Different Levels ◽  
Insight Into

Chilies are widely cultivated for their rich metabolic content, especially capsaicinoids. In our study, we determined individual sugars, organic acids, capsaicinoids, and total phenolic content in pericarp, placenta, and seeds of Capsicum annuum L., Capsicum chinense Jacq. and Capsicum baccatum L. by HPLC/MS. Dry weight varied in the cultivar ‘Cayenne’, with the first fruit having the lowest dry weight, with 4.14 g. The total sugar content and organic acid content did not vary among the fruits of all three cultivars. The cultivar ‘Cayenne’ showed differences in total phenolic and capsaicinoid content between fruits in the placenta, with the first fruit having the highest content of total phenolics (27.85 g GAE/kg DW) and total capsaicinoids (16.15 g/kg DW). Of the three cultivars studied, the cultivar ‘Habanero Orange’ showed the least variability among fruits in terms of metabolites. The content of dihydrocapsaicin, nordihydrocapsaicin, homocapsaicin, and homodihydrocapsaicin in the seeds of the second fruit was higher than that of the first fruit of the cultivar ‘Bishop Crown’. The results of our study provided significant insight into the metabolomics of individual fruits of the same chili plant. We have thus increased our understanding of how certain metabolites are distributed between fruits at different levels of the same plant and different parts of the fruit. This could be further investigated when chilies are exposed to different environmental stresses.

scholarly journals Calcium Chelated with Amino Acids Improves Quality and Postharvest Life of Lisianthus (Eustoma grandiflorum cv. Cinderella Lime)

HortScience ◽  
2015 ◽  
Vol 50 (9) ◽  
pp. 1394-1398 ◽  
Author(s):  
Reza Saeedi ◽  
Nematollah Etemadi ◽  
Ali Nikbakht ◽  
Amir H. Khoshgoftarmanesh ◽  
Mohammad R. Sabzalian
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Dry Mass ◽  
Production Quality ◽  
Control Treatment ◽  
Cut Flowers ◽  
Eustoma Grandiflorum ◽  
Postharvest Life ◽  
Calcium Sources ◽  
Nutrient Solutions

Lisianthus is one of the most important specialty cut flowers in the world. Various greenhouse conditions and inadequate evapotranspiration can disturb the transport of calcium and impair its uptake by plants. This study aimed to compare the effects of calcium amino acid chelates and calcium chloride (CaCl2) on flower production, quality, and postharvest life of cut ‘Cinderella Lime’ lisianthus. Therefore, nutrient solutions containing calcium amino acid chelates (1%) were prepared using calcium and equal concentrations of lysine, threonine, or methionine. The control treatment was a solution without amino acids and calcium. Calcium concentrations of flowering stems were significantly higher in plants treated with calcium amino acid chelates than those treated with amino acids or the control treatment. Treatment with calcium methionine chelate led to significantly higher flower numbers compared with treatment with free amino acids and the control treatment. Moreover, calcium amino acid chelates effectively improved the fresh and dry mass of the flowering stems in comparison with the control plants. In summary, among all calcium sources, calcium lysine chelate could most effectively enhance the postharvest life of lisianthus cut flowers.

scholarly journals Novel alanine serine cysteine transporter 2 (ASCT2) inhibitors based on sulfonamide and sulfonic acid ester scaffolds

2019 ◽  
Vol 151 (3) ◽  
pp. 357-368 ◽  
Author(s):  
Elias Ndaru ◽  
Rachel-Ann A. Garibsingh ◽  
YueYue Shi ◽  
Evan Wallace ◽  
Paul Zakrepine ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Binding Site ◽  
Acid Ester ◽  
Sulfonic Acid ◽  
Chemical Space ◽  
Glutamate Transporter ◽  
Homology Model ◽  
Side Chain ◽  
Large Variability

The neutral amino acid transporter alanine serine cysteine transporter 2 (ASCT2) belongs to the solute carrier 1 (SLC1) family of transport proteins and transports neutral amino acids, such as alanine and glutamine, into the cell in exchange with intracellular amino acids. This amino acid transport is sodium dependent, but not driven by the transmembrane Na+ concentration gradient. Glutamine transport by ASCT2 is proposed to be important for glutamine homoeostasis in rapidly growing cancer cells to fulfill the energy and nitrogen demands of these cells. Thus, ASCT2 is thought to be a potential anticancer drug target. However, the pharmacology of the amino acid binding site is not well established. Here, we report on the synthesis and characterization of a novel class of ASCT2 inhibitors based on an amino acid scaffold with a sulfonamide/sulfonic acid ester linker to a hydrophobic group. The compounds were designed based on an improved ASCT2 homology model using the human glutamate transporter hEAAT1 crystal structure as a modeling template. The compounds were shown to inhibit with a competitive mechanism and a potency that scales with the hydrophobicity of the side chain. The most potent compound binds with an apparent affinity, Ki, of 8 ± 4 µM and can block the alanine response with a Ki of 40 ± 23 µM at 200 µM alanine concentration. Computational analysis predicts inhibitor interactions with the binding site through molecular docking. In conclusion, the sulfonamide/sulfonic acid ester scaffold provides facile synthetic access to ASCT2 inhibitors with a potentially large variability in chemical space of the hydrophobic side chain. These inhibitors will be useful chemical tools to further characterize the role of ASCT2 in disease as well as improve our understanding of inhibition mechanisms of this transporter.

STUDIES ON THE NATURE OF RESISTANCE IN TOMATO TO CLADOSPORIUM FULVUM COOKE

1962 ◽  
Vol 40 (8) ◽  
pp. 1095-1106 ◽  
Author(s):  
D. L. Bailey ◽  
Ruth L. Lowther
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Acid Content ◽  
Causal Relation ◽  
Sugar Content ◽  
Amino Acid Content ◽  
Resistant Variety ◽  
Cladosporium Fulvum ◽  
Resistant Varieties ◽  
Host Parasite

The sugar and amino acid content of six tomato varieties were compared by paper chromatographic methods. These varieties reacted to race 1 of Cladosporium fulvum Cke. as follows: Potentate, susceptible; V-121 and Stirling Castle, resistant; Vetomold, Vinequeen, and Lycopersicon pimpinellifolium (Jusl.) Mill., immune. Potentate, Vetomold, and V-121 are closely related genetically, the last two having been developed by repeated backcrossing to Potentate of immune and resistant segregates from Potentate × L. pimpinellifolium crosses. The resistance of Stirling Castle is controlled by a gene different from the gene which conditions immunity in Vetomold and one that confers resistance on V-121, while the immunity of Vinequeen stems from L. hirsutum Humb. and Bonpl.Fructose, glucose, and sucrose occurred in all varieties, but, among the three closely related ones, the consistently low level of all three in V-121 and the low fructose in Red Currant were suggestive, while Potentate and Vetomold displayed reciprocal levels of fructose and sucrose. The two resistant varieties differed in sugar content and the three immune ones were not consistent.From four sets of extracts of all six varieties, grown under conditions optimal for disease expression, 15 amino acids were identified. The relative amounts of these differed significantly in different varieties without, however, any general causal relation to resistance being evident. The consistently lower levels of one or more of aspartic acid, serine, and glutamine in V-121 may be significant in limiting the development of the fungus in this resistant variety. The immunity of Vetomold did not seem to be conditioned by the presence or absence of specific amino acids nor did the immunity of Vinequeen. When extracts of this sort were compared with extracts from plants grown under a light experience reduced sufficiently to disturb the host–parasite relations, it was evident that this treatment significantly altered both the sugar and amino acid content of the varieties in characteristic varietal patterns. This may suggest that a suitable C/N ratio is prerequisite to compatibility. The desirability of further studies is indicated.

scholarly journals Physico-chemical Properties, Mineral Matter, Organic Acid, Amino Acid, and Plant Hormones Content of Goji Berry (Lycium barbarum L.) Grown in Turkey

2021 ◽  
Vol 9 (10) ◽  
pp. 1889-1894
Author(s):  
Rabia Talay ◽  
Ümmügülsüm Erdoğan ◽  
Metin Turan
Keyword(s):  
Amino Acid ◽  
Organic Acid ◽  
Sugar Content ◽  
Matter Content ◽  
Total Phenolic ◽  
Dry Matter Content ◽  
Lycium Barbarum ◽  
Total Sugar Content ◽  
Goji Berry ◽  
Berry Fruits

With high nutritional value, goji berry is an important fruit for human nutrition and health. Goji berry cultivation is done widely in many countries, farming has become widespread in recent years in Turkey. In study grown goji berry (Lycium barbarum L.) in Turkey are determined the physical and chemical characteristics of fruit (pH, titration acidity, dry matter content, ash content, water activity (aw) value, protein content, HMF content, total phenolic content, total sugar content, reducing sugar content, sucrose content, colour values, mineral contents, organic acid contents, amino acid contents and some plant hormone content). Goji berry fruits have pH value 5.18, titration acidity value 2.16%, dry matter content 89.40%, ash content 4.30%, water activity (aw) value 0.35, protein content 13.18%, HMF content 9.38 mg/kg, total phenolic content 9.05 mg GAE/g, total sugar content 59.26%, decreasing sugar content 57.35%, sucrose content 1.90%, L* value 40.33, a* value 22.97, b* value 33.00, C* value 40.21, H° value 55.15. In addition, the mineral content, organic acid content, amino acid content and some plant hormones of goji berry fruits were determined by analysis.

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