Antagonistic potential of some phosphate solubilizing fungi against some phyto-pathogenic fungi

Abstract Background Little phosphorus availability in soil is one of the major forces for crop production. Phosphate solubilizing fungi enhance available phosphorus from soils and contribute to achieve the plants phosphorus requirement. Phosphate solubilizing fungi are important component of soil microbiota and play an essential role in the biogeochemical cycling of phosphorus in natural ecosystems. Results The study tested the potential of the isolated phosphate solubilizing fungi to inhibit the growth of Alternaria alternata, Fusarium solani, Geotrichum candidum, Rhizoctonia solani and Sclerotium rolfsii. A total of 137 fungal isolates were isolated and identified from 30 soil samples. Nine isolates solubilized inorganic phosphate (tricalcium phosphate). Eight of these isolates were isolated from the rhizosphere of Alhagi graecorum Boiss., Allium cepa L., Ehrharta calycina Sm., Ludwigia stolonifera (Guill. & Perr.) Raven, Mentha longifolia L., Phragmites communis Trin.,Triticum aestivum L. and Zea mays L., respectively and one isolate was isolated from island's free soil. Purified single spore cultures of these nine isolates were identified to species level by multi loci DNA barcoding using internal transcribed spacer1and 2 (ITS1 and ITS 2) of the rRNA gene cluster, Calmodulin (CaM) and β-tubulin)BenA) gene markers. Conclusions The isolated phosphate solubilizing fungi belonged to two genera, Aspergillus and Penicillium. The phosphate solubilization index ranged from 1 to 1.5. Aspergillius japonicus 2 had the highest solubilization index (1.5) and also had the highest inhibition percentage (70%) against Alternaria alternata. This isolate is promising for development of biocontrol agent of Fusarium solani, Geotricum candidium and Alternaria alternata. The highest phosphate solubilization by Aspergillius japonicus 2 was obtained by using sucrose as carbon source and also ammonium chloride and tryptophan as nitrogen source. The highest solubilization percentage was 78.2% at optimum glucose concentration (5%) while the pH value that gave the highest solubilization percentage (90%) was 3. After eight days of incubation of Aspergillius japonicus 2, the solubilization percentage reached its maximum value (80.2%).

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ROCK PHOSPHATE SOLUBILIZATION AND ENZYMATIC ACTIVITIES FROM INDIGENOUS BACTERIA ON CASSAVA MILL EFFLUENT CONTAMINATED SOIL

African Journal of Health, Safety and Environment ◽

10.52417/ajhse.v1i2.83 ◽

2020 ◽

Vol 1 (2) ◽

pp. 37-51

Author(s):

C. E. Oshoma ◽

S. O. Nwodo ◽

I. S. Obuekwe

Keyword(s):

Acid Phosphatase ◽

Contaminated Soil ◽

Rock Phosphate ◽

Phosphate Solubilization ◽

Enzymatic Activities ◽

Available Phosphorus ◽

Phosphate Solubilizing Bacteria ◽

Indigenous Bacteria ◽

Rock Phosphate Solubilization ◽

Phosphate Solubilizing

The processing of cassava into value-added products is associated with discharge of effluents which contain substances that have adverse effect on the environment. Remediative activity of indigenous bacteria can be stimulated by supplementing effluents with phosphorus. Rock phosphate (RP) solubilization and enzymatic activities from bacteria on the cassava mill effluents (CME) contaminated soil was investigated. Soil mixed with varying concentrations of CME (0, 100, 200, 300, 400, 500 and 600 ml) and 10 g of RP were analyzed on days 0 and 16. Parameters analyzed were changes in pH, heterotrophic bacteria load, phosphate-solubilizing bacteria load, available phosphorus, acid phosphatase, cellulase and urease concentrations. The results showed that the medium containing 400 ml CME contaminated soil had the highest phosphate-solubilizing bacteria load (12.60 ± 2.08 x 106 cfu/ml), available phosphorus (126.00 ± 4.08 mg/kg), acid phosphatase (9.54 ± 0.51 mgN/g/min), cellulase (15.24 ± 0.81 mg/g/6h) and urease concentration (2.15±0.22 mg/g/2h). The control had the lowest phosphate-solubilizing bacteria load and enzymatic activity. Biostimulation of indigenous bacteria to enhance the degradation of cassava mill effluent-contaminated soil, using rock phosphate, showed promising results. This implies that rock phosphate solubilization by indigenous bacteria in CME-contaminated soils could be important for the remediation and reclamation of contaminated lands.

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Inorganic Phosphate Solubilization by a Novel Isolated Bacterial Strain Enterobacter sp. ITCB-09 and Its Application Potential as Biofertilizer

Agriculture ◽

10.3390/agriculture10090383 ◽

2020 ◽

Vol 10 (9) ◽

pp. 383 ◽

Cited By ~ 3

Author(s):

Gustavo Enrique Mendoza-Arroyo ◽

Manuel Jesús Chan-Bacab ◽

Ruth Noemi Aguila-Ramírez ◽

Benjamín Otto Ortega-Morales ◽

René Efraín Canché Solís ◽

...

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Inorganic Phosphate ◽

Extracellular Polymeric Substances ◽

Phosphate Solubilization ◽

Rrna Gene ◽

Bacterial Strains ◽

Habanero Pepper ◽

Phosphate Solubilizing

The excessive use of fertilizers in agriculture is mainly due to the recognized plant requirements for soluble phosphorus. This problem has limited the implementation of sustainable agriculture. A viable alternative is to use phosphate solubilizing soil microorganisms. This work aimed to isolate inorganic phosphorus-solubilizing bacteria from the soils of agroecosystems, to select and identify, based on sequencing and phylogenetic analysis of the 16S rRNA gene, the bacterium with the highest capacity for in vitro solubilization of inorganic phosphate. Additionally, we aimed to determine its primary phosphate solubilizing mechanisms and to evaluate its effect on Habanero pepper seedlings growth. A total of 21 bacterial strains were isolated by their activity on Pikovskaya agar. Of these, strain ITCB-09 exhibited the highest ability to solubilize inorganic phosphate (865.98 µg/mL) through the production of organic acids. This strain produced extracellular polymeric substances and siderophores that have ecological implications for phosphate solubilization. 16S rRNA gene sequence analysis revealed that strain ITCB-09 belongs to the genus Enterobacter. Enterobacter sp. ITCB-09, especially when immobilized in beads, had a positive effect on Capsicum chinense Jacq. seedling growth, indicating its potential as a biofertilizer.

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Phosphate solubilizing activity of Pseudomonas fluorescens PSM1 isolated from wheat rhizosphere

Journal of Applied and Natural Science ◽

10.31018/jans.v8i1.754 ◽

2016 ◽

Vol 8 (1) ◽

pp. 93-96 ◽

Cited By ~ 1

Author(s):

Anurag Yadav ◽

Kusum Yadav ◽

Anupam Vashistha

Keyword(s):

Pseudomonas Fluorescens ◽

Phosphate Solubilization ◽

Ph Value ◽

Culture Collection ◽

Phosphate Solubilizing Bacteria ◽

Linear Regression Equation ◽

Wheat Rhizosphere ◽

Bacterial Treatment ◽

Phosphate Solubilizing ◽

Solubilizing Activity

A study was designed to screen and analyze the efficient phosphate solubilizing bacteria (PSBs) from wheat rhizosphere. Five biovars of Pseudomonas fluorescens (PSM1, PSM2, PSM3, PSM4 and PSM5) were isolated from wheat rhizosphere and Bacillus megaterium MTCC 8755 procured from microbial type culture collection (MTCC) Chandigarh, India. The P. fluorescens biovar PSM1 was observed to be most efficient phosphate solubilizer. Inoculation of P. fluorescens PSM1 and B. megaterium MTCC 8755, alone and in combination, caused the highest phosphate solubilization at pH 5. At this pH, maximum phosphate solubilization was observed with B. megaterium MTCC 8755 inoculation (8.2 mg mL-1) on sixth day of incubation with P. fluorescens PSM1 (8 mg mL-1) on seventh day of incubation and with dual bacterial treatment (10.5 mg mL-1) on the fourth day of incubation. A correlation coefficient of linear regression equation of phosphate solubilization with pH indicated that pH value of the medium was directly correlated with tricalcium phosphate solubilization. The study will help in choosing soil pH specific PSB inoculant for optimizing plant growth.

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Metagenomic Analysis of Bacterial Communities in Agricultural Soils from Vietnam with Special Attention to Phosphate Solubilizing Bacteria

Microorganisms ◽

10.3390/microorganisms9091796 ◽

2021 ◽

Vol 9 (9) ◽

pp. 1796

Author(s):

Anna Hegyi ◽

Tran Bao Khuyen Nguyen ◽

Katalin Posta

Keyword(s):

Bacterial Communities ◽

Phosphate Solubilization ◽

Negative Relationship ◽

Chemical Properties ◽

Rrna Gene ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

Bacterial Phyla ◽

Physico Chemical ◽

Phosphate Solubilizing

Bacterial communities can promote increased phosphorus (P) availability for plants and microbes in soil via various mechanisms of phosphate solubilization. The production of extracellular phosphatases releases available P through the hydrolysis of organic P. Examining the abundance and diversity of the bacterial community, including phosphate solubilizing bacteria in soil, may provide valuable information to overcome P scarcity in soil ecosystems. Here, the diversity and relative abundance of bacterial phyla and genera of six agricultural soil samples from Vietnam were analysed by next generation sequencing of the 16S rRNA gene. Phosphatase activities of each soil were compared with physico-chemical parameters and the abundance of the alkaline phosphatase gene phoD. We showed the dominance of Chloroflexi, Proteobacteria, Actinobacteria, Acidobacteria and Firmicutes. Total nitrogen positively correlated with phyla Proteobacteria, Acidobacteria, Firmicutes and Planctomycetes. The abundance of several genera of Proteobacteria showed positive relationship with the copy number of the phoD gene. The abundance of several taxa positively correlated with silt content, while a negative relationship of Proteobacteria was found with sand content. Our results demonstrated the clear influence of soil physico-chemical properties on the abundance of various bacterial taxa including those potentially involved in phosphate solubilization.

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Isolation and Characterization of Phosphate Solubilizing Diazotrophic Bacteria from Rhizosphere of Rice Plant of Indo-Nepal Border

Academic Voices A Multidisciplinary Journal ◽

10.3126/av.v5i0.15856 ◽

2016 ◽

Vol 5 ◽

pp. 79-87

Author(s):

Umesh Prasad Shrivastava

Keyword(s):

Rice Plant ◽

Phosphate Solubilization ◽

Dry Weight ◽

Plant Growth Promoting Rhizobacteria ◽

Rrna Gene ◽

Isolation And Characterization ◽

Query Coverage ◽

Plant Growth Promoting ◽

Phosphate Solubilizing ◽

Bacterial Characterization

Free-living nitrogen fixing bacteria were isolated from the rhizosphere of rice plant of different regions of Indo-Nepal border. 33.78% isolates among them showed mineral phosphate solubilization. On the basis of bacterial characterization and identification with Bergey’s manual of Determinative Bacteriology (Holt et al, 1994), 38% isolates showed maximum similarity with Pseudomonas and Azotobacter, 30% with members of Enterobacteriaceae, 4% with Gram’s positive Microbacterium sp. and 28% was categorized unknown. ECI 12A isolate showed maximum solubilization index (S.I.), 0.809 on solid medium whereas 188.65 mg P/mg dry weight phosphate solubilization in liquid medium. The highest phosphate solubilizing isolate ECI 12A was sequenced after amplification of partial 16S rRNA gene, blast with BLASTn 2.2.17 program of NCBI showed 99 % identity having 99 % query coverage with Microbacterium sp. This isolate has been named as Microbacterium sp. Strain ECI-12A.The sequence of this strain has been deposited in NCBI Gene Bank under accession number EU155122 and this strain has promising potential for developing as a plant growth promoting rhizobacteria (PGPR) as well as biofertilizer.Academic Voices Vol.5 2015: 79-87

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Describing Phosphorus Sorption Processes on Volcanic Soil in the Presence of Copper or Silver Engineered Nanoparticles

Minerals ◽

10.3390/min11040373 ◽

2021 ◽

Vol 11 (4) ◽

pp. 373

Author(s):

Jonathan Suazo-Hernández ◽

Erwin Klumpp ◽

Nicolás Arancibia-Miranda ◽

Patricia Poblete-Grant ◽

Alejandra Jara ◽

...

Keyword(s):

Organic Matter ◽

Crop Production ◽

Agricultural Soils ◽

Ph Value ◽

Consumer Products ◽

Soil Samples ◽

Engineered Nanoparticles ◽

P Availability ◽

Agricultural Crop ◽

Phosphorus Sorption

Engineered nanoparticles (ENPs) present in consumer products are being released into the agricultural systems. There is little information about the direct effect of ENPs on phosphorus (P) availability, which is an essential nutrient for crop growthnaturally occurring in agricultural soils. The present study examined the effect of 1, 3, and 5% doses of Cu0 or Ag0 ENPs stabilized with L-ascorbic acid (suspension pH 2–3) on P ad- and desorption in an agricultural Andisol with total organic matter (T-OM) and with partial removal of organic matter (R-OM) by performing batch experiments. Our results showed that the adsorption kinetics data of H2PO4− on T-OM and R-OM soil samples with and without ENPs were adequately described by the pseudo-second-order (PSO) and Elovich models. The adsorption isotherm data of H2PO4− from T-OM and R-OM soil samples following ENPs addition were better fitted by the Langmuir model than the Freundlich model. When the Cu0 or Ag0 ENPs doses were increased, the pH value decreased and H2PO4− adsorption increased on T-OM and R-OM. The H2PO4− desorption (%) was lower with Cu0 ENPs than Ag0 ENPs. Overall, the incorporation of ENPs into Andisols generated an increase in P retention, which may affect agricultural crop production.

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Variabilitatea patogenității unor agenți fungici ai putregaiului de rădăcină la grâul comun de toamnă

10.53040/9789975347204.08 ◽

2020 ◽

Author(s):

Elena Sasco ◽

Keyword(s):

Common Wheat ◽

Fusarium Solani ◽

Alternaria Alternata ◽

Wheat Seedlings ◽

Culture Filtrates ◽

Growth Characters

The study established different reactions of the growth characters of the autumn common wheat seedlings to the treatment of seeds of genotypes Moldova 614, Moldova 66 and L Selania / Accent with the culture filtrates of the strains of Alternaria alternata, Drechslera sorokiniana and Fusarium solani. The 3 strains of the F. solani pathogen produced concomitant repression of root and stem in Moldova 614 and Moldova 66, but differentiated in L Selania / Accent, being identified as the most aggressive in this study. The highest sensitivity was recorded by L Selania / Accent in the case of the root under the action of Alternaria alternata strains.

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Isolation and identification of phosphate solubilizing bacteria and evaluate its effect on of Mung bean (Vigna radita [L.] R.Wilczek) growth

Iraqi Journal of Science ◽

10.24996/ijs.2019.60.5.7 ◽

2019 ◽

Vol 60 (5) ◽

pp. 985-995

Author(s):

Yusur Ramzi ◽

Hutaf A. A. Alsalim

Keyword(s):

Plant Growth ◽

Bacillus Cereus ◽

Mung Bean ◽

Phosphate Solubilization ◽

Growth Promotion ◽

Hydrolytic Enzymes ◽

Phosphate Solubilizing Bacteria ◽

Biochemical Tests ◽

Isolation And Identification ◽

Phosphate Solubilizing

Sixteen soil samples were collected from wheat, barley and yellow corn rhizosphere in Abu-Ghraib, Aqraqof, Latifieh,Tarmiah, Jadriya and of Agriculture in Baghdad university/ Baghdad city. The results found nine phosphate solubilizing bacteria (PSB) isolates (Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, Y9), formed clear zones on National Botanical Research Institute's (NBRIP) agar. The solubility index (SI) of PSB isolates ranged from 2.00 to 3.66. Y4 have the highest SI (3.66) followed by Y3 and Y6 (3.33). Phosphate solubilization abilities varying from (20.10-39.00 Î¼g.ml-1), Y4 was the highest (39.00 Î¼g.ml-1) followed by Y3 (37.00Î¼g.ml-1). The results of hydrolytic enzymes production showed that almost all nine isolates are able to produce protease and pectinase, while Y1 and Y2 showed negative results in cellulase production. Maximum ability for hydrogen cyanide (HCN) and indole acetic acid (IAA) production were showed byY3 and Y4 isolates. The isolate Y4 was found to be the most efficient isolate, so it was selected identified as Bacillus cereus using biochemical tests confirmed by VITEC 2 compact system. The results of High performance liquid chromatography (HPLC) revealed that Bacillus cereus produce oxalic acid (2.996), citric acid (9.117) and malic acid (3.734). Bacillus cereus (Y4) enhanced the growth of mung bean plants. A significant increase in branches number (12.33), plant length (83.0cm), fresh weight (27.25 g) and dry weight (1.427g) were obtained compared with control treatments. The main objective of this study is to isolate PSB and evaluate their roles in plant growth promotion. The results showed the high phosphate solubilization efficiency of PSB isolates and the identified isolates was found to be good enough for plant growth promoting.

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Contribution of Arbuscular Mycorrhizal Fungi, Phosphate–Solubilizing Bacteria, and Silicon to P Uptake by Plant

Frontiers in Plant Science ◽

10.3389/fpls.2021.699618 ◽

2021 ◽

Vol 12 ◽

Author(s):

Hassan Etesami ◽

Byoung Ryong Jeong ◽

Bernard R. Glick

Keyword(s):

Arbuscular Mycorrhizal Fungi ◽

Mycorrhizal Fungi ◽

Crop Production ◽

Current Knowledge ◽

Arbuscular Mycorrhizal ◽

P Availability ◽

Phosphate Solubilizing Bacteria ◽

P Deficiency ◽

Phosphate Solubilizing ◽

P Fertilizers

Phosphorus (P) availability is usually low in soils around the globe. Most soils have a deficiency of available P; if they are not fertilized, they will not be able to satisfy the P requirement of plants. P fertilization is generally recommended to manage soil P deficiency; however, the low efficacy of P fertilizers in acidic and in calcareous soils restricts P availability. Moreover, the overuse of P fertilizers is a cause of significant environmental concerns. However, the use of arbuscular mycorrhizal fungi (AMF), phosphate–solubilizing bacteria (PSB), and the addition of silicon (Si) are effective and economical ways to improve the availability and efficacy of P. In this review the contributions of Si, PSB, and AMF in improving the P availability is discussed. Based on what is known about them, the combined strategy of using Si along with AMF and PSB may be highly useful in improving the P availability and as a result, its uptake by plants compared to using either of them alone. A better understanding how the two microorganism groups and Si interact is crucial to preserving soil fertility and improving the economic and environmental sustainability of crop production in P deficient soils. This review summarizes and discusses the current knowledge concerning the interactions among AMF, PSB, and Si in enhancing P availability and its uptake by plants in sustainable agriculture.

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Mycoflora associated with two species of Liliaceae in Bangladesh

Dhaka University Journal of Biological Sciences ◽

10.3329/dujbs.v22i1.46272 ◽

2013 ◽

Vol 22 (1) ◽

pp. 39-46

Author(s):

Shamim Shamsi ◽

Najmun Naher

Keyword(s):

Fusarium Solani ◽

Alternaria Alternata ◽

New Record ◽

Macrophomina Phaseolina ◽

Fungal Species ◽

Colletotrichum Orbiculare ◽

Lasiodiplodia Theobromae ◽

Colletotrichum Capsici ◽

Colletotrichum Dematium

A total of nine fungi were isolated from two ornamental angiosperms, namely Hemerocallis fulva L. and Pancratium verecundum Ant. belong to Liliaceae. Three species of fungi were found to be associated with the leaf of Hemerocallis fulva. The fungi were Colletotrichum capsici, (Syd.) Bull. & Bisby, Colletotrichum dematium (Pers. Ex Fr.) and Glomerella montana (Sacc.)v Arx & E. Muller. Six species of fungi, namely Alternaria alternata (Fries) Keissler, Colletotrichum orbiculare (Berk & Mont.) Arex., Curvularia clavata Jain, Fusarium solani (Mort.) Sacc., Lasiodiplodia theobromae (Pat.) Griffon and Maubol and Macrophomina phaseolina (Tassi) Goid were found to be associated with P. verecundum. Among the fungal species Glomerella montana is new record for Bangladesh. Dhaka Univ. J. Biol. Sci. 22(1): 39-46, 2013 (January)

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