Adhesion molecules of blood polymorphonuclear leukocytes and alveolar macrophages in rats: modulation by exposure to ozone

1999 ◽  
Vol 18 (9) ◽  
pp. 547-551 ◽  
Author(s):  
E Hoffer ◽  
Y Baum ◽  
A Tabak ◽  
C Frevert
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Alveolar Macrophages ◽  
Peripheral Blood ◽  
Cell Adhesion Molecules ◽  
Polymorphonuclear Leukocytes ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Selectin Family ◽  
Integrin Family

1 Exposure to elevated levels of ozone results in an infiltration of polymorphonuclear leukocytes (PMNs) into the lungs. The purpose of this study was to investigate whether the ozone-induced inflammatory process is preceded by a change in the expression of adhesion molecules (integrins and selectins) in peripheral blood PMNs and alveolar macrophages in rats. 2 Female Sprague Dawley rats were exposed to air or ozone (1 p.p.m., 2 h). Bronchoalveolar lavage (BAL) was carried out and blood was collected via intracardiac puncture at 0 or 18 h after the exposure. There were no PMN in the BAL fluid at time 0 after the 2 h exposure to ozone. The expression of cell adhesion molecules from the integrin family (represented by CD18) on alveolar macrophages (AM) was lowered. The expression of cell adhesion molecules from the selectin family (represented by CD62L) on blood PMN was not affected by exposure to ozone, while the expression of integrins (CD11b) on blood PMN was lowered. 3 This effect was confirmed by experiments in which plasma of ozone-exposed animals was incubated with PMN from peripheral blood obtained from nonexposed animals. In these experiments, the expression of CD11b on PMNs of non-exposed animals was lower after incubation with plasma from ozone-exposed animals. 4 Our experiments suggest the presence of factor(s) in blood, which cause a decrease in the expression of CD11b on PMNs.

Neutrophils launch monocyte extravasation by release of granule proteins

2009 ◽  
Vol 102 (08) ◽  
pp. 198-205 ◽  
Author(s):  
Alma Zernecke ◽  
Christian Weber ◽  
Oliver Soehnlein
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Polymorphonuclear Leukocytes ◽  
Blood Stream ◽  
Fine Tuning ◽  
Special Importance ◽  
Inflammatory Monocytes ◽  
Granule Proteins ◽  
Early Recruitment

SummaryDuring their journey from the blood stream to sites of inflammation polymorphonuclear leukocytes (PMN) release a wide panoply of granule proteins. Shortly after the PMN efflux, the extravasation of monocytes sets in and recent research provides evidence that the release of PMN granule proteins and monocyte extravasation are causally interrelated. Granule proteins seeded on the endothelium by adherent PMN allow direct activation and subsequent adhesion of monocytes. In addition, PMN granule components enhance the endothelial expression of cell adhesion molecules, efficiently supporting the arrest of monocytes at inflamed vessels. Moreover, granule proteins contribute to the fine tuning of the local chemokine network. Proteolytic modification of chemokines as well as enhancement of local chemokine synthesis lead to increased monocyte extravasation. Finally, PMN granule proteins exert direct chemotactic effects, a mechanism which is of special importance in the early recruitment of inflammatory monocytes. Hence, granule proteins modify the monocyte extravasation cascade in a multifaceted manner ensuring the efficiency of these mechanisms.

Modification of Cytokine Levels and CD34+ Stem Cell Adhesion Molecules during Mobilization in Multiple Myeloma (MM) Patients.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 5068-5068
Author(s):  
Patrizia Zappasodi ◽  
Alessandro Corso ◽  
Chiara Rusconi ◽  
Mara De Amici ◽  
Erica Consensi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Peripheral Blood ◽  
Cell Adhesion Molecules ◽  
Serum Levels ◽  
High Baseline ◽  
Cytokine Levels ◽  
Baseline Levels

Abstract The mechanisms underlying the mobilization of peripheral blood stem cells (PBSC) are still unknown, even though studies on healthy donors and on cancer patients have hypothesised a central role for CD34+ stem cells adhesion molecules and for chemokines serum levels. The type of mobilizing regimen and the pathologic condition can exert different effects on cytokine levels and adhesion molecules expression. To evaluate the modification of these parameters during mobilization we studied 10 MM patients treated with DCEP chemotherapy (Decadron, Cyclophosphamide, Etoposide, cis-Platin) followed by G-CSF (5 mg/kg/day starting 48 hours after chemotherapy until PBSC collection). The expression of four CD34+ cell adhesion molecules (Thy1, L-selectin, VLA4, CXCR4) was measured in the bone marrow before mobilization therapy and in the leukapheresis product. Serum levels of five cytokines (TGF-β, IL-8, HGF, VEGF-A, sVCAM-1) were assessed, through specific ELISA kits, before therapy, 5 days after the G-CSF stimulation and at stem cells collection. Each parameter was correlated with the number of CD34+ cells collected. Thy1 significantly decreased (p=0.007) and L-selectin significantly increased (p=0.038) in nearly all cases. By contrast, no statistically significant differences were observed for VLA4 and CXCR4 due to a consistent variability. Serum levels of IL-8 showed an increase at day 5, then a significant decrease at the time of the harvest (p=0.013); VEGF-A constantly increased (p<0.001) and TGF-β decreased (p=0.001). The modifications registered for sVCAM-1 and HGF did not reach statistical significance. The expression of CD34+ adhesion molecules did not correlate with the number of PBSC collected, while among cytokines, high baseline levels of TGF-β and VEGF-A significantly correlated with high number of CD34+ stem cells yielded (p=0.016, p=0.042 respectively). In conclusion, this study shows that during stem cells mobilization in MM Thy-1 expression significantly decreases while L-selectin increases; the behaviour of VLA-4 and CXCR4 is extremely variable. As regards cytokines, we found a statistically significant increase of VEGF-A level, while TGF-β, which induces the stem cell homing, decreases during the mobilization allowing stem cells to migrate in the peripheral blood. High baseline levels of TGF-β and VEGF-A predict a good stem cells harvest.

scholarly journals PO-050 Effects of Exercise on Gene Expression Correlative to Bone Metabolism in Peripheral Blood Mononuclear Cells

2018 ◽  
Vol 1 (3) ◽  
Author(s):  
Yongjie Yang ◽  
Li Gao
Keyword(s):  
Gene Expression ◽  
Cell Adhesion ◽  
Bone Metabolism ◽  
Adhesion Molecules ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Cell Adhesion Molecules ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Objective To examine the gene expression pattern of Peripheral Blood Mononuclear Cells （PBMCs）and to explore exercise-sensitive genes correlative to bone metabolism in PBMCs. Thus to provide a theoretical basis for exercise intervention to prevent and treat osteoporosis.  Methods Uphill (+8° ) and downhill (-8° ) training were used for the exercise loading in two-month-old male Sprague-Dawley rats. The exercise method performed at 25 m/min, training 50 min/d, 5 d/wk, for 12 wk, respectively. Bone mineral density of distal femurs was measured using dual X-ray absorptiometry, and the expressed gene profile of PBMCs was examined using Gene Chip IVT Labeling Kit (Affymetrix).  Results Compared with control (CON) group, the BMD of the femur in the downhill (DOWN) group was significantly increased. Compared with the uphill （UP）group, the BMD and BMC of the femur in the DOWN group were significantly increased. There were 38 genes detected differentially expressed between two exercise groups together with CON group. The expression of genes modified by running involved in immunity, cell proliferation，Rheumatoid arthritis，Cell adhesion molecules and Tnf signaling pathway. There were 105 differently expressed genes between the DOWN group and the UP group which were mainly enriched in biological processes and pathways such as response to hydrogen peroxide，lipopolysaccharide，cell factor and  mechanical stimulus，Cell adhesion molecules，cell migration，collagen biosynthetic process and Tnf signaling pathway. Tnf, Cxcl2, Ccl2, Jun and Mmp9 as the key nodes of protein interaction network were identified as candidate genes related to bone metabolism and sensitive to exercise.  Conclusions With weight gaining，age increasing and training time prolonging, long-term and high-intensity exercise will be harm for bone. At the same time and same running speed, downhill running conduces to increase bone density more than uphill running. It may be associated with differential expression of exercise-sensitive genes involved in bone metabolism in PBMCs.

Variations in the expression of cell-adhesion molecules on liver-associated lymphocytes and peripheral-blood lymphocytes in patients with and without liver metastasis

1995 ◽  
Vol 61 (4) ◽  
pp. 475-479 ◽  
Author(s):  
María García-Barcina ◽  
Ioseba Bidaurrazaga ◽  
Véronique Neaud ◽  
Paulette Bioulac-Sage ◽  
Charles Balabaud ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Liver Metastasis ◽  
Adhesion Molecules ◽  
Peripheral Blood ◽  
Cell Adhesion Molecules ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes
Sign in / Sign up

Export Citation Format

Share Document