Extracts from the Leaves of Chromolaena odorata A Potential Agent for Wound Healing

Chromolaena odorata (L.) King and Robinson (Siam weed) extract has been used to stop bleeding and in wound healing in many tropical countries. However, its detailed mechanisms have not been elucidated. In this study, we examined the molecular mechanisms by which Siam weed extract (SWE) affected hemostatic and wound healing activities. SWE promoted Balb/c 3T3 fibroblast cell migration and proliferation. Subsequently, we found that heme oxygenase-1 (HO-1), the accelerating wound healing enzyme, was increased at the transcriptional and translational levels by SWE treatments. The HO-1 promoter analyzed with luciferase assay was also increased by treatment of SWE in a dose-dependent manner. This induction may be mediated by several kinase pathways including MEK, p38MAPK, AKT, and JNK. Quantitative real-time PCR using undifferentiated promonocytic cell lines revealed that thromboxane synthase (TXS), a potent vasoconstrictor and platelet aggregator, was increased and MMP-9, an anti platelet aggregator, was decreased in the presence of SWE. Our studies presented that SWE accelerated hemostatic and wound healing activities by altering the expression of genes, including HO-1, TXS, and MMP-9.

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Nanoencapsulation of Chromolaena odorata Extract Using Pluronic F127 as an Effectively Herbal Delivery System for Wound Healing

Journal of Nanomaterials ◽

10.1155/2021/6663986 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Ngoc-Dung Huynh Luu ◽

Le Hang Dang ◽

Hoang Minh Bui ◽

Trang Thuy Thi Nguyen ◽

Bich Tram Nguyen ◽

...

Keyword(s):

Wound Healing ◽

Blood Clot ◽

Fibroblast Cell ◽

Ethyl Acetate Fraction ◽

Negative Control ◽

Pluronic F127 ◽

Chromolaena Odorata ◽

Long Term Storage ◽

Copolymer Micelles

Chromolaena odorata is a medicinal herb with prominent pharmacological properties. The therapeutic efficiency of Chromolaena odorata extracts and its ingredients have, however, been limited by various factors, including the lack of targeting capacity and poor bioavailability. To approach this drawback, ethyl acetate fraction extract of Chromolaena odorata- (EA.ChO-) encapsulated pluronic-based nanocarriers was disclosed herein. The most common pluronic triblock copolymer micelles (pluronic F127) was used for the nanosized formulation of Chromolaena odorata extract. The obtained results show that EA.ChO-encapsulated nanoparticles have a spherical morphology with a designed hydrodynamic size was about 183.7 nm and zeta potential -39.5 mV. The EA.ChO nanoparticles are stable in different aqueous solutions (water, PBS 2.8, and PBS 7.4). The lyophilized form of the EA.ChO nanoparticles exhibited excellent stability for long-term storage. Notably, the EA.ChO nanoparticles were 1.3-1.4 fold more effective in the growth of fibroblast than the free EA.ChO, verifying the potential of pluronic F127 nanoparticles to the increased function of EA.ChO in the proliferation of fibroblast cell. In addition, bleeding stopped within 55 ± 6 s which was 20 s faster than that of free EA.ChO and 38-44 s faster than that of negative control treatments. The EA.ChO nanoencapsulation processed a rapid blood clot formation compared to control, free EA.ChO, pluronic F127, and water, suggesting the excellent bioavailability of EA.ChO nanoencapsulation. The obtained results thus provided a promising prospect for raising the activity Chromolaena odorata extract in wound healing application.

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Comparative Study of the in vitro Antioxidant Properties of Methanolic Extracts of Chromolaena odorata and Ageratum conyzoides used in Wound Healing

International Annals of Science ◽

10.21467/ias.6.1.8-12 ◽

2018 ◽

Vol 6 (1) ◽

pp. 8-12 ◽

Cited By ~ 3

Author(s):

Babatunde Oso ◽

Nosarieme Abey ◽

Oyedotun Oyeleke ◽

Boyede Olowookere

Keyword(s):

Wound Healing ◽

Antioxidant Properties ◽

Lipid Peroxides ◽

First Aid ◽

Antioxidant Potential ◽

Chromolaena Odorata ◽

Ageratum Conyzoides ◽

Methanolic Extracts ◽

Therapeutic Value

This study sought to evaluate the potential antioxidative potencies of Chromolaena odorata and Ageratum conyzoides, commonly used herbs in Nigeria for wound first aid and healing. The antioxidant potentials were evaluated by measuring their abilities to scavenge radicals, reduce oxidised iron and quench the formation of lipid peroxides. The findings showed that the extract of Ageratum conyzoides exhibited significantly higher (p<0.05) antioxidant potential than the extract of Chromolaena odorata. The study, therefore, suggests that Ageratum conyzoides has higher potential for therapeutic value than Chromolaena odorata in terms of antioxidant potential.

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Botanical Potency of Chromolaena odorata Linn (Hagonoy) as Mosquitocidal

International Journal of Science and Healthcare Research ◽

10.52403/ijshr.20211033 ◽

2021 ◽

Vol 6 (4) ◽

pp. 228-234

Author(s):

John Michael Sasan ◽

Rengee May Lumantao ◽

Carl Laurence Magallon

Keyword(s):

Wound Healing ◽

Natural Product ◽

Environmental Conditions ◽

Cardiac Glycosides ◽

Cost Effective ◽

Mosquito Population ◽

Chromolaena Odorata ◽

Potential Significance ◽

Mosquitocidal Activity ◽

Environmental Weed

Chromolaena odorata Linn is considered an environmental weed that is traditionally used as a treatment of numerous environmental conditions such as wound healing functions and amelioration of inflammation. In this study, Chromolaena odorata linn was discovered as an alternative and natural product of mosquitocide. Based on the results gathered and obtained the stem, leaves, and roots are proven to be effective to use rather than using only one part of the plant. Furthermore, the data gathered shows that it only took a small amount of time to kill the mosquito using all parts of the Chromolaena odorata linn extract. The mosquitocidal activity of Chromolaena odorata linn had shown its potential significance, eco-friendly, and cost-effective with the presence of botanical phytochemicals (alkaloids, cardiac glycosides, anthocyanin, tannin, and flavonoids) as a source of controlling the mosquito population Keywords: botanical potency, mosquitocidal, chromolaena odorata Linn, mosquitos.

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Efficacy of Chromolaena odorata leaf extracts for the healing of rat excision wounds

Veterinární Medicína ◽

10.17221/161/2016-vetmed ◽

2017 ◽

Vol 62 (No. 10) ◽

pp. 565-578 ◽

Cited By ~ 5

Author(s):

K. Vijayaraghavan ◽

J. Rajkumar ◽

MA Seyed

Keyword(s):

Wound Healing ◽

Inhibitory Activity ◽

Soft Tissues ◽

Healing Process ◽

Lipid Peroxide ◽

Wound Healing Process ◽

Phytochemical Screening ◽

Chromolaena Odorata ◽

Excision Wound ◽

Ethanolic Extracts

Injury to the soft tissues is followed by wound healing, which consists of four stages: haemostasis, inflammation, proliferation and remodelling. Chromolaena odorata is a weed that is traditionally used for the treatment of various ailments in humans and animals. The present study was aimed at exploring the wound healing potential of aqueous and ethanolic extracts of C. odorata in a rat excision wound model. This investigation involved phytochemical screening and in vitro analyses of various parameters such as antioxidant activity, lipid peroxide inhibitory activity and the effects of extracts on contraction and epithelialisation of the rat excision wounds. The phytochemical screening of both ethanolic and aqueous extracts showed that they were rich in secondary metabolites such as alkaloids, flavonoids, tannins, saponins, terpenoids, anthraquinones, cardiac glycosides and carbohydrates. The aqueous extract showed high antioxidant and lipid peroxide inhibitory activity, while the ethanolic extract showed high total phenol content and hydrogen peroxide inhibitory activity at concentrations of 50, 100 and 250 μg/ml. Our results also indicate that the most effective concentration of the C. odorata extract for excision wound healing was 5.0% (w/w). C. odorata-treated groups exhibited a faster reduction in wound area compared to control and Betadine-treated groups. In addition, the topical application of C. odorata extract increased collagen synthesis and its stabilisation at the wound site, as evidenced by the increase in hydroxyproline and hexosamine levels and expression of collagen. The present investigation demonstrates that aqueous and ethanolic extracts of C. odorata of varying concentrations promote an accelerated wound healing process and might represent a novel healing agent. Our findings are of potential clinical relevance and might be highly beneficial for drug discovery and development in the area of both human and veterinary medicine.

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Acceleration of angiogenesis in wound healing after tooth extraction with kirinyuh (Chromolaena odorata) leaf extract

BIO Web of Conferences ◽

10.1051/bioconf/20214107001 ◽

2021 ◽

Vol 41 ◽

pp. 07001

Author(s):

Yosaphat Bayu Rosanto ◽

Vinka Ardhiyanti

Keyword(s):

Wound Healing ◽

Blood Vessels ◽

Guinea Pigs ◽

Tooth Extraction ◽

Leaf Extract ◽

Ethanol Extract ◽

Positive Control ◽

Chromolaena Odorata ◽

Mandibular Incisor ◽

Topical Gel

Tooth extraction is dental procedure that causes wound in oral cavity. Kirinyuh leaves (Chromolaena odorata) contain flavonoids, saponins and tannins which help the wound healing after tooth extraction. Purpose of study was to determine effect of giving kirinyuh leaf ethanol extract on wound healing after tooth extraction in guinea pigs (Cavia cobaya) in angiogenesis. Sixty male guinea pigs were divided into five groups: aquadest, iodine glycerine (positive control), kirinyuh 2.5%, 5%, and 10%. Each group consisted of 12 guinea pigs in each group. Kirinyuh leaf extract was prepared in a topical gel with CMC-Na as the solvent. Left mandibular incisor of guinea pigs was extracted then treated according to the group every day. Guinea pigs were euthanized on days 3, 7, 10, and 14 (three guinea pigs from each group per day) and made histological sections with hematoxylin eosin staining. Angiogenesis was observed with binocular microscope equipped with camera. Results showed that number of new blood vessels in kirinyuh 10% group reached highest number faster (day 7) than the other groups (p=0.000). This study concluded that kirinyuh leaves accelerate angiogenesis by increasing the number of blood vessels. The 10% concentration is the most effective kirinyuh extract concentration to accelerate angiogenesis.

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Tekelan Leaves (Chromolaena odorata) Infusion and 10% Povidone-Iodine on Incision Wound Healing Process of Mice (Mus musculus) Infected with Staphylococcus aureus

World s Veterinary Journal ◽

10.54203/scil.2021.wvj8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Arif Caesar Budi ◽

Iwan Sahrial Hamid ◽

Djoko Legowo

Keyword(s):

Staphylococcus Aureus ◽

Wound Healing ◽

Mus Musculus ◽

Povidone Iodine ◽

Healing Process ◽

Wound Healing Process ◽

Skin Area ◽

Chromolaena Odorata ◽

Synthetic Drugs ◽

Significant Difference

Skin is considered to be the most prone organ to injury and infection compared to other body organs. Skin infections are most commonly caused by Staphylococcus aureus. The most commonly used synthetic drugs to overcome skin problems is Povidone-iodine. Considering the Indonesian traditional method, injuries could be cured by dripping Tekelan leaves (Chromolaena odorata) infusion on the wounded skin area. This study aimed to compare the treatment effects of Tekelan leaves (Chromolaena odorata) infusion and povidone-iodine in the wound healing process of mice skin. The subjects of this study were 25 mice (Mus musculus) which were incised and infected with Staphylococcus aureus in 5 groups, namely, P0 (without treatment), P1 (povidone-iodine 10%), P2 (Tekelan infusion 5%), P3 (Tekelan infusion 10%), and P4 (Tekelan infusion 20%). Observations were made microscopically on wound conditions based on a total assessment of the four observed parameters (epithelization, inflammation rate, connective tissue proliferation, and angiogenesis). The results showed that the total observation value in the P0 and P2 groups was lower than the other groups. The P1 group obtained the highest score. The Kruskal-Wallis test showed a significant difference among the treatment groups (p < 0.05). While the Mann Whitney test indicated P1 had a significant difference with P0, P2, and P3, it had no significant difference with P4. It can be concluded that the Tekelan Leaves (Chromolaena odorata) infusion can be used as an alternative of povidone-iodine with an optimal concentration of 20%.

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