scholarly journals Acceleration of angiogenesis in wound healing after tooth extraction with kirinyuh (Chromolaena odorata) leaf extract

2021 ◽  
Vol 41 ◽  
pp. 07001
Author(s):  
Yosaphat Bayu Rosanto ◽  
Vinka Ardhiyanti
Keyword(s):  
Wound Healing ◽  
Blood Vessels ◽  
Guinea Pigs ◽  
Tooth Extraction ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Chromolaena Odorata ◽  
Mandibular Incisor ◽  
Topical Gel

Tooth extraction is dental procedure that causes wound in oral cavity. Kirinyuh leaves (Chromolaena odorata) contain flavonoids, saponins and tannins which help the wound healing after tooth extraction. Purpose of study was to determine effect of giving kirinyuh leaf ethanol extract on wound healing after tooth extraction in guinea pigs (Cavia cobaya) in angiogenesis. Sixty male guinea pigs were divided into five groups: aquadest, iodine glycerine (positive control), kirinyuh 2.5%, 5%, and 10%. Each group consisted of 12 guinea pigs in each group. Kirinyuh leaf extract was prepared in a topical gel with CMC-Na as the solvent. Left mandibular incisor of guinea pigs was extracted then treated according to the group every day. Guinea pigs were euthanized on days 3, 7, 10, and 14 (three guinea pigs from each group per day) and made histological sections with hematoxylin eosin staining. Angiogenesis was observed with binocular microscope equipped with camera. Results showed that number of new blood vessels in kirinyuh 10% group reached highest number faster (day 7) than the other groups (p=0.000). This study concluded that kirinyuh leaves accelerate angiogenesis by increasing the number of blood vessels. The 10% concentration is the most effective kirinyuh extract concentration to accelerate angiogenesis.

Effects of 1% and 3% Mobe Leaf Extract Gel on Socket Wound Healing after Tooth Extraction

2021 ◽  
Vol 24 (1) ◽  
pp. 1-5
Author(s):  
Olivia Avriyanti Hanafiah ◽  
Denny Satria ◽  
Avi Syafitri
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Leaf Extract ◽  
Alveolar Bone ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Gel Treatment

Tooth extraction is a process of removing teeth from the alveolar bone. In wound healing, fibroblast are very important cells. The main purpose of this study was to determine the effect of mobe leaf 1% and 3% extract gel (Artocarpus lakoocha) on fibroblast proliferation in post extraction tooth socket wound healing. This research used 16 samples of wistar rats, divided into 4 groups, a positive control group, a negative control group and a 1% and 3% mobe leaf extract gel group. The left mandibular incisors were extracted, then 1% and 3% gels of mobe leaf extract were applied on day 1 to day 7. Data analysis was calculated using the Kruskal-Wallis test on clinical data and one way ANOVA test for microscopic. The result of the socket wound healing activity test for a good concentration of mobe leaf extract gel was 3%. This research shows significant resultith p-value of 0.018 (< 0.05) on the closure of the socket wound clinically which means the closure of the wound accelerates because of the mobe leaf 3% extract gel treatment. The distance of fibroblast on microscopically shows significant resultith a p-value of 0.002 (< 0.05), which means that there was an enlargement of the distance fibroblast at the socket wound closure with application of mobe leaf 3% extract gel. From the results of the study it can be concluded that mobe leaf 3% extract gel has the best ability to show acceleration the closure of the socket wound either clinically or microscopically.

scholarly journals Pengaruh gel ekstrak daun binahong (Anredera cordifolia) 5% terhadap peningkatan osteoblas pada proses penyembuhan luka pasca pencabutan gigi tikus strain WistarEffect of 5% binahong (Anredera cordifolia) leaf extract in increasing the osteoblast amount at the tooth extraction wound healing process of Wistar rat

2020 ◽  
Vol 32 (1) ◽  
pp. 9
Author(s):  
Jannatus Salis Sa'diyah ◽  
Dewi Ayu Septiana ◽  
Nanda Nailul Farih ◽  
Juwita Raditya Ningsih
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design

Pendahuluan: Pencabutan gigi di dunia kedokteran gigi adalah tindakan yang sering dilakukan. Pasca terjadinya pencabutan gigi, maka tulang pada soket akan rusak. Penyembuhan tulang ditandai dengan terbentuknya sel-sel osteoblas baru. Daun binahong merupakan daun yang mengandung flavonoid, alkaloid, terpenoid, asam askorbat, saponin, tanin dan asam oleanolik. Kandungan dari daun binahong dapat meningkatkan jumlah osteoblas pasca pencabutan gigi. Tujuan dari penelitian adalah menganalisis pengaruh pemberian gel ekstrak daun binahong (Anredera cordifolia) konsentrasi 5% terhadap peningkatan osteoblas pada proses penyembuhan luka pasca pencabutan gigi. Metode: Jenis penelitian ini merupakan penelitian eksperimental laboratorium murni dengan rancangan post test only control group design. Subyek penelitian berupa 45 ekor tikus dibagi menjadi 3 kelompok, yaitu kelompok gel ekstrak daun binahong, kontrol negatif dan kontrol positif. Gigi insisivus sentral kiri rahang bawah dilakukan pencabutan gigi kemudian diaplikasikan gel ekstrak daun binahong 5%, CMC-Na 1% dan iod gliserin. Tikus diterminasi pada hari ke 3,5,7,14 dan 21 pasca dilakukan pencabutan gigi untuk selanjutnya dibuat preparat histologis dengan pengecatan hematoksilin eosin. Jumlah sel osteoblas dihitung dengan optilab dan software image raster. Hasil: Hasil uji parametrik one way anova menunjukan terdapat perbedaan jumlah osteoblas yang bermakna antar kelompok (p<0,05) pada masing-masing hari. Hasil LSD (Least Significance Different) menunjukkan terdapat perbedaan yang bermakna (p<0,05) pada hari ke 5,7, 14 dan 21 antara kelompok perlakuan dengan kontrol negatif dan terdapat perbedaan yang bermakna pada hari ke 7 dan 14 antara kelompok perlakuan dan kontrol positif. Simpulan: Gel ekstrak daun binahong konsentrasi 5% berpengaruh dalam meningkatkan jumlah sel osteoblas pasca dilakukan pencabutan gigi.Kata kunci: Pencabutan gigi, gel ekstrak daun binahong, osteoblas. ABSTRACTIntroduction: Tooth extraction is the most common treatment in dentistry. After tooth extraction, the bone in the socket will be damaged. The formation of new osteoblast characterises bone healing. Binahong leaves contain flavonoids, alkaloids, terpenoids, ascorbic acid, saponins, tannins and oleanolic acids. This research was aimed to investigate the effectivity of binahong (Anredera cordifolia) leaf extract gel with a concentration of 5% in increasing the number of osteoblasts at the tooth extraction wound healing process. Methods: This research was an experimental laboratory with a post-test only control group design. Forty-five rats were divided into 3 groups; binahong leaf gel group, negative control group, and positive control group. Mandibular left central incisive was extracted, then binahong leaf gel, 1% CMC-Na, and glycerin were applied on the wound socket in different groups. All rats then terminated at day 3, 5, 7, 14, and 21 post-extraction and processed for the histological examination then coloured by haematoxylin-eosin. The number of osteoblasts was calculated by Optilab and raster image software. Results: The ANOVA test result showed significant differences in the number of osteoblasts among groups (p<0.05) on each day. The LSD (Least Significance Different) test result showed significant differences (p<0.05) at day 5, 7, 14, and 21 post-extraction, between binahong leaf gel group compared to the negative control group, and also at day 7 and 14 post-extraction between binahong leaf gel group compared to the positive control group. Conclusion: Binahong leaf extract gel with a concentration of 5% is effective in increasing the number of osteoblast in the wound healing process after tooth extraction.Keywords: Tooth extraction, Binahong leaf extract gel, osteoblast.

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals Potential of Malacca leaf (Phyllanthus emblica) against Salmonella sp

2020 ◽  
Vol 151 ◽  
pp. 01029
Author(s):  
Nuzul Asmilia ◽  
Mahdi Abrar ◽  
Yudha Fahrimal ◽  
Amalia Sutriana ◽  
Yobeswi Husna
Keyword(s):  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Positive Control ◽  
Hexane Extract ◽  
Clear Zone ◽  
Inhibitory Property

Malacca is one of traditional medicine that possesses a potent antimicrobial activity. This study aims to determine the inhibitory activity of Malacca leaf extract on the growth of Salmonella sp in vitro. The bacteria was obtained from Microbiology Laboratory of the Faculty of Veterinary Medicine, Universitas Syiah Kuala. The study was conducted using n-hexane extract, ethyl acetate extract and ethanol of malacca leaves with dilution concentrations of 5%, 25%, and 50%.The inhibitory property of malacca leaf was tested using Kirby-Bauer method. Data were analyzed descriptively. The results of this study indicate that n-hexane extract, ethyl acetate extract and ethanol extract of malacca leaves can inhibit the growth of Salmonella sp. The n-hexane extract of malacca leaves showed a greater inhibition than the ethyl acetate and ethanol extract of malacca leaves. n-hexane extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 1.35 mm (weak), 4.97 mm (moderate), and 12.87 mm (strong), respectively ethyl acetate extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 2.00 mm (weak), 5.72 mm (moderate), and 7.58 mm (moderate), whereas in ethanol extract were 0.47 mm (weak), 2.58 mm (weak), and 4.35 mm (weak), repectively. The clear zone areas in negative and positive control were 0.00 mm 20.00 mm, respectively. Malacca leaf extract possess inhibitory property against the growth of the Salmonella sp.

scholarly journals PENGUJIAN EFEKTIVITAS DIURETIK KOMBINASI EKSTRAK ETANOL DAUN AFRIKA (Gymnanthemum amygdalinum Del.) DAN DAUN KELAPA SAWIT (Elaeis guineensis Jacq.) PADA TIKUS JANTAN

2020 ◽  
Vol 3 (1) ◽  
pp. 106-111
Author(s):  
Delisma Simorangkir
Keyword(s):  
Leaf Extract ◽  
Elaeis Guineensis ◽  
Urine Volume ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Treatment Groups ◽  
White Rats ◽  
Group 2 ◽  
Palm Leaf

African Leaf and palm leaf crops have a secondary metabolite content such as alkaloids and flavonoids that are efficacious as diuretics. The purpose of this study is to know the effectiveness of diuretics from the combination  African leaf ethanol extract and palm leaf ethanol extract. Methods used Eksperiemental. The test of diuretic activity is conducted divided into 6 treatment groups. Each group consists of 3 tails of white rats. Group 1 (positive control) administered Furosemide 3.6 mg/kgBB, Group 2 (negative control) was given suspension Na-CMC 0.5%, group 3 without treatment as well as groups of 4.5, and 6 in a row administered a combination of African leaf extract doses and extracts Oil palm leaf ethanol in a row of "50 mg/kgBB + 67, 5mg/kgBB"; "100mg/kgBB + 135 mg/kgBB"; and "200 mg/kgBB + 270 mg/kgBB". Each mouse is then given a 20ml NaCl/kgBB as an oral loading dose. Measured urine volume is recorded every hour for 6 hours after it continues to measure urine volume. The results showed that the combination of African leaf ethanol extract dosage and palm leaf ethanol extract had a diuretic effect if it was compared with negative control. The conclusion of the study was the results of the study showed that the combination of African leaf extract dosage and palm leaf extract is the most effective dose of Group 6 (African leaf extract 200 mg/kgBB + palm leaves 270 mg/kgBB ). Because it produces the highest urine volume of 23.01 ml.

scholarly journals Uji aktivitas gel ekstrak daun pohpohan (Pilea trinervia W.) terhadap penyembuhan luka bakar pada kelinci (Oryctolagus cuniculus)

2017 ◽  
Vol 5 (2) ◽  
pp. 75
Author(s):  
Via Fitria ◽  
Rafiki Fahrul Arifin ◽  
Nia Kurniasih
Keyword(s):  
Wound Healing ◽  
Leaf Extract ◽  
Positive Control ◽  
Test Results ◽  
Activity Test ◽  
Pressure Test ◽  
Spreading Test ◽  
Wound Healing Activity ◽  
Non Parametric

<p align="center"><strong>Abstrak</strong></p><p align="center"><strong> </strong></p><p>Masyarakat Indonesia mengenal dan memanfaatkan tanaman berkhasiat obat sebagai salah satu upaya dalam penanggulangan masalah kesehatan. Salah satu tanaman berkhasiat untuk menyembuhkan luka bakar adalah daun pohpohan (<em>Pilea trinervia </em>W.). Penelitian ini bertujuan menguji aktivitas gel ekstrak daun pohpohan (<em>Pilea trinervia W</em>.) terhadap penyembuhan luka bakar pada kelinci. Pembuatan gel dilakukan pada beberapa konsentrasi ekstrak daun pohpohan, yaitu 0,5, 1, dan 2%, Setelah dilakukan pembuatan gel, maka dilakukan evaluasi gel untuk mengetahui kualitas sediaan, meliputi uji organoleptis, homogenitas, uji pH, uji daya sebar, uji daya lekat, dan uji aktivitas luka bakar. Uji aktivitas luka bakar dilakukan pada 3 ekor kelinci jantan dengan 5 sisi perlakuan pada tiap kelinci, dan diinduksi dengan logam panas dengan alat uji Rafiky’sTool Pressure Test. Sebagai pembanding digunakan Bioplacenton®. Pemberian gel pada pengobatan luka bakar dilakukan 2 kali sehari. Parameter yang diukur adalah diameter luka bakar selama 10 hari. Data hasil uji aktivitas luka bakar diuji statistik menggunakan uji non parametric Kruskal-Wallis dan uji lanjut Mann Whitney. Hasil evaluasi gel menunjukkan bahwa gel yang mengandung ekstrak daun pohpohan konsentrasi 0,5, 1, dan 2% memenuhi persyaratan homogenitas, pH, daya penyebaran dan daya lekat. Hasil uji aktivitas luka bakar menunjukkan bahwa gel dengan konsentrasi ekstrak pohpohan 0,5, 1, dan 2% mampu menurunkan diameter luka bakar lebih cepat dibandingkan kontrol. Kelompok ekstrak daun pohpohan 2% memiliki aktivitas penyembuhan luka bakar paling cepat dan hampir sama dengan kontrol positif Bioplacenton®. Dapat disimpulkan bahwa gel yang mengandung ekstrak pohpohan mempunyai aktivitas menyembuhkan luka bakar.</p><p> </p><p><strong>Kata kunci:</strong>     Daun pohpohan (<em>Pilea trinervia</em> W.), Gel, Luka bakar</p><p> </p><p align="center"><strong><em>Wound healing activity of pohpohan </em></strong><strong>(<em>Pilea trinervia </em>W.) </strong><strong><em>extract gel on rabbit</em></strong></p><p align="center"><strong><em> </em></strong></p><p align="center"><strong><em>Abstract</em></strong></p><p align="center"><strong><em> </em></strong></p><p><em>Indonesian people recognize and utilize nutritious crops as one of the efforts in the prevention of health problems. One of the nutritious plants to heal burns is the leaves of pohpohan (Pilea trinervia W.). This study aims to test the activity of pohpohan leaf extract (Pilea trinervia W.) on the healing of burns in rabbits. Preparation of gel is done on some concentration of pohpohan leaf extract, that is 0,5, 1, and 2%. After gel making, gel evaluation is done to know the quality of preparation, including organoleptic test, homogeneity, pH test, spreading test, sticky, and burn activity test. Burning activity test was performed on 3 male rabbits with 5 treatment sides on each rabbit, and induced with hot metal by Rafiky'sTool Pressure Test. For comparison use Bioplacenton®. Gels on the treatment of burns done 2 times a day. Parameters measured were burn diameter for 10 days. Data on burn activity test results were tested statistically using non-parametric Kruskal-Wallis test and further test of Mann Whitney. The results of gel evaluation showed that the gel containing pohpohan leaves extract concentration of 0.5, 1, and 2% fulfilled the homogeneity, pH, dispersion and stickiness requirements. The result of burn activity test showed that gel with concentration of 0,5, 1, and 2% extract pohpohan able to reduce burn burn diameter faster than control. The 2% pohpohan leaf extract group has the fastest burn healing activity and almost equal to the positive control of Bioplacenton®. It can be concluded that gel containing pohpohan extract has activity to heal burns.</em></p><p><em> </em></p><p><strong><em>Keywords:</em></strong><em>  P</em><em>ohpohan (Pilea trinervia W.), Gel, Wound healing</em></p>

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DARI EKSTRAK ETANOL DAUN Mimosa pudica Linn. MENGGUNAKAN METODE DPPH

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 106
Author(s):  
Wulan Wulan ◽  
Adithya Yudistira ◽  
Henki Rotinsulu
Keyword(s):  
Vitamin C ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Antioxidant Compounds ◽  
Phytochemical Screening ◽  
Mimosa Pudica ◽  
Average Percentage ◽  
Dpph Method ◽  
High Antioxidant Activity

ABSTRACT Antioxidant are compounds that can inhibit the oxidation rate of other molecules or neutralize free radicals. Putri Malu Leaf (Mimosa pudica Linn.) is not yet widely known as a medicinal plant. Through phytochemical screening, Mimosa pudica Linn. leaves known to have antioxidant compounds such as flavonoids, alkaloids, terpenoids, saponins and coumarin. This study aims to determine how much the antioxidant content in the extract of Mimosa pudica Linn. leaves using DPPH method with a concentration of 100 mg/L, 75 mg/L, 50 mg/L and 25 mg/L and Vitamin C as a positive control. Each sampel was made three repetitions of the test. The test uses a UV-Vis Spectrophotometer. The highest results were obtained from the concentration of 100 mg/L in the second repetition of 89.74%. The highest average percentage  of yield obtained is at a concentration of 100 mg/L. Based on the result obtained from Mimosa pudica Linn. leaf extract seems like that the ethanol extract of Mimosa pudica Linn. leaves has high antioxidant activity. Keywords: Antioxidant, DPPH, Mimosa pudica Linn.  ABSTRAK Antioksidan  ialah senyawa yang mampu menghambat laju oksidasi molekul lain atau menetralisir radikal bebas. Daun Putri Malu (Mimosa pudica Linn.) belum dikenal luas sebagai tanaman obat.Melalui skrining fitokimia, daun Mimosa pudica Linn.diketahui memiliki senyawa antioksidan seperti flavonoid, alkaloid, terpenoid, saponin dan kumarin. Penelitian ini bertujuan untuk mengetahui seberapa besar kandungan antioksidan didalam ekstrak tanaman daun Mimosa pudica Linn.menggunakan metode DPPH dengan konsentrasi 100 mg/L, 75 mg/L, 50 mg/L dan 25 mg/L dan Vitamin C sebagai kontrol positif. Masing-masing sampel dibuat tiga kali pengulangan uji.Pengujian menggunakan alat Spektrofotometer UV-Vis. Terlihat hasil tertinggi diperoleh dari konsentrasi 100 mg/L pada pengulangan ke-II yaitu sebesar 89,74%. Rata-rata persentase hasil yang diperoleh tertinggi ialah pada konsentrasi 100 mg/L. Berdasarkan hasil yang diperoleh dari ekstrak daun Mimosa pudica Linn.terlihat bahwa ekstrak etanol daun Mimosa pudica Linn. memiliki aktivitas antioksidan yang tinggi.  Kata Kunci : Antioksidan, DPPH,Mimosa pudica Linn.

HAIR TONIC FORMULATION OF CLOVE LEAVES (SYZYGIUM AROMATICUM) ETHANOL EXTRACT AND THE EFFECTIVENESS ON RABBIT HAIR GROWTH

2020 ◽  
pp. 245-248
Author(s):  
HAFIZHATUL ABADI ◽  
HANAFIS SASTRA WINATA ◽  
PARHAN ◽  
VIVI EULIS DIANA ◽  
ADEK CHAN ◽  
...  
Keyword(s):  
High Temperatures ◽  
Hair Growth ◽  
Leaf Extract ◽  
Physical Stability ◽  
Ethanol Extract ◽  
Positive Control ◽  
Syzygium Aromaticum ◽  
Climatic Chamber ◽  
Stability Tests ◽  
Concentration Variation

Objective: The purpose of this study was to determine the physical stability and effectiveness of hair growth in hair tonic preparations containing ethanol extract of clove leaves (Syzygium aromaticum) with a concentration variation of 5, 10, and 15%. Methods: Formulations were evaluated, including organoleptic observation, pH checking, and preparation stability tests using the Climatic Chamber. The effectiveness of clove leaf extract as a hair tonic was tested by looking at hair growth in test animals. Results: The results showed that the preparation was stable at high temperatures (40±2 °C) where the odor, color, clarity, and pH remained constant. Effectiveness test on rabbit hair growth showed significantly different results after measuring hair growth on the 21st day, where the results were 11.83+0.06 mm for positive control, 1.71+0.06 mm as blank, 1.69+0.06 mm for the group without treatment, and successively the results of the group given clove leaf extract 5, 10, and 15% were 4.00+0.06; 4.80+0.06; and 5.46+0.08 mm. Conclusion: Hair tonics containing clove ethanol extract can increase rabbit hair growth.

scholarly journals Histological Assessment of Palatal Donor Site Wound Healing after Application of Moringa oleifera Lamarck Leaf Extract in Rats

2019 ◽  
Vol 13 (02) ◽  
pp. 248-254 ◽  
Author(s):  
Amaliya Amaliya ◽  
Regina Kumala Muhaimina ◽  
Agus Susanto ◽  
Afifah B. Sutjiatmo
Keyword(s):  
Wound Healing ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Donor Site ◽  
Hydroxypropyl Methylcellulose ◽  
Punch Biopsy ◽  
Sprague Dawley ◽  
Topical Gel ◽  
Sprague Dawley Rats ◽  
Excisional Wound

Abstract Objective Palatal excisional wound healing in 60 Sprague–Dawley rats was studied for a period of 14 days. Materials and Methods The wounds were made with a punch biopsy instrument with a diameter of 4 mm. After wounding, the test groups received a topical gel of Moringa oleifera Lamarck 2% or 4% leaf extract, whereas control groups received povidone iodine gel and hydroxypropyl methylcellulose (HPMC 4%). Three rats were killed at 0, 3, 7, 10, and 14 days after wounding from each allocated group. The wounds were assessed at different control times and light microscopy was employed to view serial sections from blocks with magnification. Results Histological examination revealed that group treated with M. oleifera Lamarck 4% leaf extract showed the highest fibroblast synthesis and collagen deposition. Conclusion This study suggests that M. oleifera Lamarck leaf extract could be developed as a therapeutic agent for wound healing.

scholarly journals Efek Pemberian Ekstrak Daun Pegagan (Centella Asiatica) Terhadap Penyembuhan Luka Sayat Pada Tikus Putih Jantan (Rattus Norvegicus) Galur Wistar

2020 ◽  
Vol 9 (1) ◽  
pp. 21
Author(s):  
I Made Subhawa Harsa
Keyword(s):  
Wound Healing ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Centella Asiatica ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
White Rats ◽  
Wistar Strain

Wound refers to partly damage or loss of body tissue. One way that has been used by the people in helping the process of wound healing is the use of medicinal plants, such as gotu kola (Centella asiatica). Gotu kola (Centella asiatica)  is kind of plant that found almost in all territories of Indonesia which functions as a cell revitalizing substance which quickens wound healing those are burnt, skin ulcer and to prevent keloid. This study is aimed to know the effect of giving gotu kola leaf extract (Centella Asiatica) against to the healing of the wound incision on male white rats (Rattus Norvegicus) wistar strain. This study is a pure experimental research using the randomized post test only control group design with the subjects are 30 male white rats (Rattus norvegicus) wistar strain that were divided into 3 groups, namely K1, K2, and K3. The incision wound were made on the back of all white rat in each group with a length of ± 2 cm and a depth of ± 2 mm. The wound in the negative control group (K1) was not given any substance; in the positive control group (K2) was given povidone iodine 10%; and in the treatment group (K3) was given gotu kola leaf extract at a dose of 37,5 mg/150 grBB. The study was done for 10 days. Furthermore, wound observation and monitoring is held. Then the results were processed by using One Way ANOVA. The result study of wound incision healing on male white rats (Rattus norvegicus) wistar strain shows that there is significant difference among the negative control group (K1), the positive control group (K2), and the treatment group (K3) on One Way ANOVA test which significant p-value are 0,027 (p < 0.05). The result of Post-Hoc LSD test also shows a significant differences among the K1 and K3 groups, and K2 and K3 groups which significant p-values are 0,011 and 0,038 (p < 0,05). From these results, it can be concluded that gotu kola leaf extract (Centella asiatica) effective on quickens wound healing of the wound incision on male white rats (Rattus norvegicus) wistar strain.

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