scholarly journals Elevated Plasma Concentration of Asymmetric Dimethylarginine That Is Reduced by Single Dose Testosterone Administration in Idiopathic Hypogonadotropic Hypogonadism Patients

2005 ◽  
Vol 90 (3) ◽  
pp. 1651-1654 ◽  
Author(s):  
Erdinc Cakir ◽  
Omer Ozcan ◽  
Halil Yaman ◽  
Emin Ozgur Akgul ◽  
Cumhur Bilgi ◽  
...  
Keyword(s):  
Single Dose ◽  
Asymmetric Dimethylarginine ◽  
Hypogonadotropic Hypogonadism ◽  
Control Group ◽  
No Production ◽  
Elevated Plasma ◽  
Idiopathic Hypogonadotropic Hypogonadism ◽  
Blood Samples ◽  
Testosterone Administration ◽  
Plasma Nitrite

Our aim was to investigate whether plasma l-arginine and asymmetric dimethylarginine (ADMA) concentrations and nitric oxide (NO) production are altered in male idiopathic hypogonadotropic hypogonadism (IHH) patients in the hypogonadal state and after single dose testosterone administration compared with those in control subjects. Eighteen newly diagnosed male patients with IHH and 20 healthy volunteer controls matched by age and body mass index were enrolled in the study. Single dose testosterone was administrated im. Initially, pretreatment blood samples were collected after overnight fasting. Posttreatment blood samples were drawn 10 d after the injection. ADMA, l-arginine, and NO were measured in pre- and posttreatment blood samples. The pretreatment ADMA and l-arginine levels were significantly higher, and plasma nitrite plus nitrate (NOx) levels were lower than those in the control group. After 10 d of treatment, ADMA and l-arginine levels were significantly reduced, and NOx levels were significantly increased. There was a significant positive correlation (P < 0.01) between ADMA and l-arginine and a negative correlation between ADMA and NOx levels in patients and controls. In conclusion, the patients with IHH showed elevated plasma ADMA levels associated with a reduction in NO production. Single dose parenteral T administration lowered ADMA concentrations and increased NO production to the control group values.

scholarly journals The Impact of LHR Gene Polymorphism Rs12470652 in Women with POF and Nihh, A Case-Control Study

2020 ◽  
pp. 508-516
Author(s):  
Samer Thamir Mahmoud Mahmoud ◽  
Abdul Kareem A. Alkazaz ◽  
Bushra J. Al-Musawi
Keyword(s):  
Real Time ◽  
Premature Ovarian Failure ◽  
Hypogonadotropic Hypogonadism ◽  
Ovarian Failure ◽  
Luteinizing Hormone Receptor ◽  
Control Group ◽  
Idiopathic Hypogonadotropic Hypogonadism ◽  
Blood Samples ◽  
The Impact ◽  
Fertile Women

The current study was designed to investigate the impact of the missense Single Nucleotide Polymorphism (SNP), Asn291Ser (c.872A>G: rs12470652), of LHR gene (Luteinizing hormone receptor gene) in peripheral blood samples of Iraqi infertile women diagnosed with premature ovarian failure (POF) and normosmic idiopathic hypogonadotropic hypogonadism(niHH, patients with normal sense of smell). Following the hormonal analysis, fifty women diagnosed with premature ovarian failure and fifty women diagnosed with normosmic idiopathic hypogonadotropic hypogonadism were included as patient groups, while fifty healthy fertile women were enrolled as a control group. The blood samples were obtained from patient and control groups at Kamal Al-Samarrai Hospital in Baghdad, Iraq through March to December 2018. The genotyping of the SNP (rs12470652) was carried out by real-time polymerase chain reaction (real-time PCR) of the purified genomic DNA obtained from All-cell-pellet (ACP) of blood samples. The frequency of the LHR (rs12470652), p.Asn291Ser minor allele G, was found to be 32% healthy fertile women, 30 % of those with POF (p = 0.879), and 28% in the niHH patients (p = 0.644) Therefore, no statistically significant differences were found. This research aims to study the relationship between polymorphism in the region of LHR gene position: 872 A > G, (Asn291Ser), (rs12470652), and both premature ovarian failure and normosmic idiopathic hypogonadotropic hypogonadism. Therefore, the potential influence of (rs12470652) p.Asn291Ser polymorphism on both types of female infertility of an Iraqi population was evaluated. In conclusion, no impact was observed since the conducted study on a sample population of Iraqi women showed that the prevalence of LHR (rs12470652) polymorphisms did not significantly differ in the two infertile patients groups (POF and niHH) as compared to the healthy fertile control group.

PROLACTIN IN MAINTENANCE OF THE CORPUS LUTEUM OF EARLY PSEUDOPREGNANCY IN THE GOLDEN HAMSTER

1981 ◽  
Vol 90 (2) ◽  
pp. 145-150 ◽  
Author(s):  
K. H. HARRIS ◽  
B. D. MURPHY
Keyword(s):  
Single Dose ◽  
Corpus Luteum ◽  
Golden Hamster ◽  
Peripheral Blood ◽  
Plasma Levels ◽  
Progesterone Level ◽  
Control Group ◽  
Blood Samples

The role of prolactin in the maintenance of the corpus luteum of pseudopregnancy was studied in the golden hamster. Nine groups of seven to fourteen animals each received 1 mg bromocriptine at 11.00 h on days 1, 2 or 3 of pseudopregnancy (three groups for each day). On each day of treatment with bromocriptine, one group of hamsters was injected with bovine prolactin 4 h before bromocriptine, and one group received prolactin 4 h before bromocriptine for three consecutive days following treatment with bromocriptine. One group received bromocriptine only. These nine groups were compared with a control group of animals given 0·85% saline instead of bromocriptine and prolactin. Peripheral blood samples were taken from all hamsters at 11.00 h on days 3, 4, 5 and 6 of pseudopregnancy and plasma levels of progesterone were determined by radioimmunoassay. Luteolysis, indicated by a decline in progesterone level by 24 or 48 h after treatment with bromocriptine, occurred in all hamsters given bromocriptine alone, whether it was administered on day 1, 2 or 3. Pretreatment with a single dose of prolactin did not mitigate the bromocriptine-induced fall in progesterone. In the majority of cases, pretreatment with prolactin plus daily doses of prolactin maintained the progesterone at levels not different from saline-treated hamsters. These data suggest that prolactin is a necessary luteotrophin during early pseudopregnancy without which luteolysis ensues.

scholarly journals An investigation of the protective effects of Dehydroepiandrosterone (DHEA) in chemotherapatic Cyclophosphamide (CP) induced ovarian damage on rats

2019 ◽  
Vol 6 (12) ◽  
pp. 340-346
Author(s):  
Önder Sakin ◽  
Yasemin Alan ◽  
Ali Doğukan Anğın ◽  
Kahyan Basak ◽  
Murat Alan
Keyword(s):  
Single Dose ◽  
Ovarian Tissue ◽  
Test Group ◽  
Antral Follicle ◽  
Control Group ◽  
Protective Effects ◽  
Blood Samples ◽  
Vascular Congestion ◽  
Ovarian Damage ◽  
Total Damage

Objective: We aimed to investigate whether cyclophosphamide-induced damage to rat ovary can be prevented by DHEA. Material and Method: Group 1 (the control Group): no treatment was administered. Intact ovarian tissue was removed and blood samples were taken for anti-mullerian hormone (AMH) test. Group 2 (the Cyclophosphamide Group): Rats received Cyclophosphamide intraperitoneally at a single dose of 150 mg / kg. Group 3 (the Cyclophosphamide + DHEA Group): Rats received Cyclophosphamide intraperitoneally at a single dose of 150 mg / kg at baseline and DHEA subcutaneously for 10 days at a dose of 60 mg / kg daily. Rats in groups 2 and 3 were sacrificed at the end of 10 days, ovarian tissues were removed and blood samples were taken for AMH test. Results: While normal ovarian tissue damage scores were zero, cyclophosphamide showed significant damage and histopathological changes in all rats. Cyclophosphamide group had higher vascular congestion (p=0.004) and total damage scores (p=0.010) than normal ovarian group. Cyclophosphamide + DHEA group had higher edema (p<0.001), vascular congestion (p<0.001) and total damage scores (p<0.001). Cyclophosphamide group had a decrease in primordial (p = 0.001), primary (p = 0.043) and preantral follicles(p = 0.006). Cyclophosphamide + DHEA group showed a decrease in primordial (p = 0.001) and antral follicles(p = 0.018). AMH levels did not decrease in both groups. Conclusions: It was found that the use of DHEA to prevent Cyclophosphamide-induced ovarian damage in rats did not produce significant changes in antral follicle counts, ovarian volume, and AMH levels, which were important for clinical practice.

scholarly journals Investigated of vitreous and serum Asymmetric Dimethylarginine levels in diabetic retinopathy

2019 ◽  
Author(s):  
Atilla Alpay ◽  
Özlem Özcan ◽  
Sılay Cantürk Uğurbaş ◽  
Suat Hayri Uğurbaş N
Keyword(s):  
Diabetic Retinopathy ◽  
Peripheral Blood ◽  
Asymmetric Dimethylarginine ◽  
Diabetic Group ◽  
Control Group ◽  
Adma Level ◽  
Blood Samples ◽  
Significant Difference ◽  
The Mean

Abstract Purpose To investigate the intravitreal and blood Asymmetric dimethylarginine (ADMA) level in diabetic retinopathy (DR). Materials and Methods Twenty eyes of 20 patients with DR with the decision of vitrectomy, and 20 eyes of 20 patients without diabetes as a control group were included in the study. During vitrectomy, vitreous and minimum 3ml peripheral blood samples were taken simultaneously. Serum and vitreous ADMA levels were compared between the two groups. Results The serum ADMA level was higher in the diabetic group compared to the non-diabetic group (p=0,009). However, there was no statistically significant difference between the mean vitreous ADMA levels. There was no correlation between serum and vitreous ADMA levels. Conclusion In patients with diabetic retinopathy, serum ADMA levels increased, but no increase in vitreous ADMA levels was detected.

The effects of thyroxine and triiodothyronine on the thyrotrophin response to thyrotrophin-releasing hormone in normal and iopanoic acid-treated euthyroid rats

1982 ◽  
Vol 101 (2) ◽  
pp. 205-209 ◽  
Author(s):  
Rubén Boado ◽  
Edgardo Ulloa ◽  
Angel A. Zaninovich
Keyword(s):  
Body Weight ◽  
Single Dose ◽  
Normal Saline ◽  
Fold Increase ◽  
Control Group ◽  
Thyrotrophin Releasing Hormone ◽  
Blood Samples ◽  
Releasing Hormone ◽  
Iopanoic Acid

Abstract. The present work studied the TSH response to TRH in T4- and T3-treated normal rats and in rats with depressed intrapituitary conversion of T4 to T3. Euthyroid normal rats were injected iv with a single dose of 6 μG T4/100 g body weight, 1 μg T3/100 g or normal saline. Another group treated with iopanoic acid (IOP) received 1 or 10 μg T4/100 g, 0.21 or 2.5 μg T3/100 g, or saline as control. Twenty min later 1 μg TRH/100 g was injected iv. Blood samples were drawn at times 0, 20 min (immediately preceding the injection of TRH) and 30 min (10 min post-TRH) for measurement of plasma T4, T3 and TSH. Other groups of IOP-treated rats were injected iv with 150 μCi of [3',5'-125I]T4; the hypophyses were removed at 30 min and homogenized in PBS buffer, extracted and chromatographed in tertiary amyl alcohol:hexane:ammonia. The control group of rats had a 13-fold increase in plasma TSH 10 min after the TRH injection, while rats treated with 6 μg T4/100 g had a 7.6-fold increase (P < 0.01 vs control increment) and rats treated with 1 μg T3/100 g had no change in TSH response as compared to controls. In IOP-treated rats injected with 10 μg T4/100 g there was a smaller increase in the TSH response to TRH as compared to control (P < 0.01), and a similar smaller response was seen in rats treated with 2.5 μg T3/100 g (P < 0.01 vs control). IOP-treated animals given 1 μg T4 or 0.21 μg T3/100 g had no significant changes in TSH response to TRH. It seems probable that T4 possesses an intrinsic hormonal capacity to regulate the secretion of TSH before it monodeiodinates to T3.

scholarly journals Oral single dose of allopurinol in thoroughbred foals born from mares with placentitis

2016 ◽  
Vol 46 (6) ◽  
pp. 1119-1125
Author(s):  
Luciana Oliveira de Araujo ◽  
Carlos Eduardo Wayne Nogueira ◽  
Fernanda Maria Pazinato ◽  
Friedrich Frey Junior ◽  
Silvano Costa Paixão ◽  
...  
Keyword(s):  
Single Dose ◽  
Adverse Effects ◽  
Serum Calcium ◽  
Group Treatment ◽  
Control Group ◽  
Blood Samples ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Wbc Count ◽  
Thoroughbred Foals

ABSTRACT: The aim of this study was to evaluate the effects of Allopurinol in foals born from mares with placentitis. Twenty foals were assigned into two groups: Healthy foals (n=10), born from healthy mares and Placentitis foals (n=10), born from mares with placentitis. Five foals from each group were randomly assigned to a treatment or control group. Treatment groups received Allopurinol (40mg kg-1 orally six hours after birth). Blood samples were collected for estimation of hematological variables and serum concentration of calcium, chloride, creatinine, phosphorus, glucose, lactate and magnesium. Placentitis foals presented leukopenia and neutropenia when compared with Healthy foals, at birth. The white blood cell (WBC) count was lower in the Placentitis foals untreated at 12 hours. No adverse effects related to the use of Allopurinol were detected. Treated Placentitis foals showed higher serum calcium and glucose levels within 12 hours than untreated Placentitis foals. Administration of Allopurinol PO in foals born from mares with placentitis did not result in adverse effects and can help in stabilizing serum calcium and glucose levels.

Reduction of enantioselectivity in the kinetic disposition and metabolism of verapamil in rats exposed to toluene

2008 ◽  
Vol 86 (5) ◽  
pp. 232-239 ◽  
Author(s):  
F.H. Mateus ◽  
J.S. Lepera ◽  
M.P. Marques ◽  
V.B. Boralli ◽  
V.L. Lanchote
Keyword(s):  
Single Dose ◽  
Oxidative Metabolism ◽  
Wistar Rats ◽  
Plasma Concentrations ◽  
Wilcoxon Test ◽  
Control Group ◽  
Chiral Drugs ◽  
Blood Samples ◽  
Male Wistar Rats ◽  
Air Control

Toluene and verapamil are subject to extensive oxidative metabolism mediated by CYP enzymes, and their interaction can be stereoselective. In the present study we investigated the influence of toluene inhalation on the enantioselective kinetic disposition of verapamil and its metabolite, norverapamil, in rats. Male Wistar rats (n = 6 per group) received a single dose of racemic verapamil (10 mg/kg) orally at the fifth day of nose-only toluene or air (control group) inhalation for 6 h/day (25, 50, and 100 ppm). Serial blood samples were collected from the tail up to 6 h after verapamil administration. The plasma concentrations of verapamil and norverapamil enantiomers were analyzed by LC-MS/MS by using a Chiralpak AD column. Toluene inhalation did not influence the kinetic disposition of verapamil or norverapamil enantiomers (p > 0.05, Kruskal–Wallis test) in rats. The pharmacokinetics of verapamil was enantioselective in the control group, with a higher plasma proportion of the S-verapamil (AUC 250.8 versus 120.4 ng·h·mL–1; p ≤ 0.05, Wilcoxon test) and S-norverapamil (AUC 72.3 versus 52.3 ng·h·mL–1; p ≤ 0.05, Wilcoxon test). Nose-only exposure to toluene at 25, 50, or 100 ppm resulted in a lack of enantioselectivity for both verapamil and norverapamil. The study demonstrates the importance of the application of enantioselective methods in studies on the interaction between solvents and chiral drugs.

A case of idiopathic hypogonadotropic hypogonadism which attained remission by LH and FSH treatment

2013 ◽  
Author(s):  
Yui Watanabe ◽  
Takeshi Hayashi ◽  
Hiroyuki Yamazaki ◽  
Katsuyoshi Tojo ◽  
Kazunori Utsunomiya
Keyword(s):  
Hypogonadotropic Hypogonadism ◽  
Idiopathic Hypogonadotropic Hypogonadism

Mutations in HS6ST1 are causal in self-limited delayed puberty as well as idiopathic hypogonadotropic hypogonadism

2015 ◽  
Author(s):  
Sasha Howard ◽  
Ariel Poliandri ◽  
Helen Storr ◽  
Louise Metherell ◽  
Claudia Cabrera ◽  
...  
Keyword(s):  
Hypogonadotropic Hypogonadism ◽  
Delayed Puberty ◽  
Idiopathic Hypogonadotropic Hypogonadism

Liver, Kidney Function Tests and Oxidative Damage During and after Treatment of Salmonella typhimurium Infection in Experimental Local Rabbits

2018 ◽  
Vol 9 (4) ◽  
Author(s):  
Mustafa Salah Hasan ◽  
Ayman Barzan Abdulgafor ◽  
Maher Saber Owain ◽  
Mohammed Ali Hussein ◽  
Qusay Mohammed Aboud ◽  
...  
Keyword(s):  
Single Dose ◽  
Oxidative Damage ◽  
Salmonella Typhimurium ◽  
Infected Group ◽  
Post Treatment ◽  
Kidney Damage ◽  
Control Group ◽  
Post Inoculation ◽  
Group 5 ◽  
Group 2

This study aimed to evaluate the liver, kidney damage caused by S. typhimurium and to estimate the oxidative damage in association with this bacteria. A highly virulent isolates of S. typhimurium were obtained from the department of internal and preventive medicine/ College of Veterinary Medicine/ University of Baghdad. A twenty five local rabbits of both genders with age range (2-4 months) weeks old were used for this study, the rabbits were divided randomly into five groups each group contains 5 rabbits :- group 1: drenched orally with 5 ml of normal saline and consider as control group, group 2: were drenched orally with (5 ml) suspension which contain (5��109 CFU) of Salmonella typhimurium and regarded as infected group, group 3 were drenched orally with (5 ml) suspension which have (5��109 CFU) of Salmonella typhimurium then treated with a single dose of gentamicin alone at 0.05ml/kg (5mg/ml) orally after presence of signs (after 24hrs. post inoculation), group 4 were drenched (5 ml) suspension having (5��109 CFU) of Salmonella typhimurium then treated with a single dose of Ca-EDTA alone at 40mg/kg orally after presence of signs (after 24hrs. post inoculation) and group 5 were drenched (5 ml) suspension that contain (5��109 CFU) of Salmonella typhimurium then treated with a single dose of combined gentamicin at 0.05ml/kg (5mg/ml) orally after presence of signs (after 24hrs. post inoculation) and Ca-EDTA 40mg/kg after presence of signs (after 24hrs. post inoculation).The results of biochemical profile showed a significant increase (p less than 0.05) in ALT, creatinine and urea levels in infected group as compared with control group, while, the treated groups especially group 5 showed a significant improvement in ALT, Urea and creatinine levels which returned to relative normal levels as compared with infected group after 96hrs. post treatment. Also, the results of oxidative stress showed a significant increase in the levels of MDA in G2, G3, G4 and G5 after 48 hrs. post treatment, while the level of GSH showed a significant decrease in the level at 48hrs., both were returned to relative normal levels after 96hrs.post treatment especially in group 5.In conclusion, S. typhimurium can causing liver and kidney damage which is manifested by increase ALT, Urea and Creatinine. Also, MDA and GSH is increased due to salmonellosis.

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