C/EBP  (CCAAT/Enhancer Binding Protein) Controls Cell Fate Determination during Mammary Gland Development

2000 ◽  
Vol 14 (3) ◽  
pp. 359-368 ◽  
Author(s):  
T. N. Seagroves
Keyword(s):  
Mammary Gland ◽  
Cell Fate ◽  
Mammary Gland Development ◽  
Binding Protein ◽  
Cell Fate Determination ◽  
Fate Determination ◽  
Enhancer Binding Protein ◽  
Ccaat Enhancer Binding Protein ◽  
Gland Development

C/EBPβ (CCAAT/Enhancer Binding Protein) Controls Cell Fate Determination during Mammary Gland Development

2000 ◽  
Vol 14 (3) ◽  
pp. 359-368 ◽  
Author(s):  
Tiffany N. Seagroves ◽  
John P. Lydon ◽  
Russell C. Hovey ◽  
Barbara K. Vonderhaar ◽  
Jeffrey M. Rosen
Keyword(s):  
Mammary Gland ◽  
Cell Fate ◽  
Binding Protein ◽  
Mammary Glands ◽  
Mammary Development ◽  
Mrna Levels ◽  
Wild Type ◽  
Cell Fate Decisions ◽  
Enhancer Binding Protein ◽  
Ccaat Enhancer Binding Protein

Abstract Deletion of the transcription factor CCAAT/enhancer binding protein (C/EBP)β results in a severe inhibition of lobuloalveolar development in the mouse mammary gland. Because progesterone receptor (PR) is requisite for alveolar development, the expression of PR was investigated in C/EBPβ−/− mice. Unexpectedly, the number of PR-positive cells, as well as the levels of PR mRNA, were elevated 3-fold in the mammary glands of C/EBPβ−/− mice. Furthermore, in contrast to wild-type nulliparous mice, in which PR distribution shifted from a uniform to nonuniform pattern between 8–12 weeks of age, C/EBPβ−/− mice exhibited uniform PR distribution throughout all stages of mammary development analyzed. No change in C/EBPβ mRNA levels was observed in the mammary glands of PR−/− mice, suggesting that PR acts in a pathway either in parallel to or downstream of C/EBPβ. The overexpression and disrupted cellular distribution of PR in C/EBPβ−/− mice were coincident with a striking 10-fold decrease in cell proliferation after acute steroid hormone treatment, assayed by incorporation of bromodeoxyuridine. In wild-type mice, PR and bromodeoxyuridine-positive cells were adjacent to each other and rarely colocalized. No differences in the level or pattern of PR expression were observed in the uterus, suggesting that C/EBPβ influences PR in a mam-mary-specific fashion. Together, these data suggest that C/EBPβ may control cell fate decisions in the mammary gland through the appropriate temporal and spatial expression of molecular markers, such as PR, that induce the proliferation of alveolar progenitor cells via juxtacrine mechanisms.

scholarly journals Expression of the genes encoding CCAAT-enhancer binding protein isoforms in the mouse mammary gland during lactation and involution

1998 ◽  
Vol 334 (1) ◽  
pp. 205-210 ◽  
Author(s):  
Georgios SABATAKOS ◽  
Gareth E. DAVIES ◽  
Maria GROSSE ◽  
Anthony CRYER ◽  
Dipak P. RAMJI
Keyword(s):  
Transcription Factors ◽  
Mammary Gland ◽  
Dna Binding ◽  
Binding Protein ◽  
Mouse Mammary Gland ◽  
Binding Activity ◽  
Protein Isoforms ◽  
Dna Binding Activity ◽  
Enhancer Binding Protein ◽  
Ccaat Enhancer Binding Protein

Transcription factors belonging to the CCAAT-enhancer binding protein (C/EBP) family have been implicated in the activation of gene expression in the mammary gland during lactation. We have therefore investigated the detailed expression profile of the C/EBP family during lactation and involution of the mouse mammary gland. The expression of C/EBPβ and C/EBPδ mRNA was low during lactation, increased dramatically at the beginning of involution and remained constant thereafter. In contrast, C/EBPα mRNA expression was relatively high during the early stages of lactation, declined to low levels during the late stages of lactation and at the start of involution, and increased again during involution. Electrophoretic mobility-shift assays showed a close correlation between the expression of the C/EBP genes and the functional C/EBP DNA-binding activity and, additionally, demonstrated the participation of heterodimers, formed from among the three proteins, in DNA–protein interactions. The DNA-binding activity of the activator protein 1 (AP1) family of transcription factors was also induced during involution. These results therefore point to potentially important regulatory roles for both the C/EBP and the AP1 family during lactation and involution of the mammary gland.

scholarly journals A noncoding RNA is a potential marker of cell fate during mammary gland development

2006 ◽  
Vol 103 (15) ◽  
pp. 5781-5786 ◽  
Author(s):  
M. R. Ginger ◽  
A. N. Shore ◽  
A. Contreras ◽  
M. Rijnkels ◽  
J. Miller ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Cell Fate ◽  
Noncoding Rna ◽  
Mammary Gland Development ◽  
Potential Marker ◽  
Gland Development

Cold-inducible RNA binding protein in mouse mammary gland development

2016 ◽  
Vol 48 (6) ◽  
pp. 577-587 ◽  
Author(s):  
Daniel A. Lujan ◽  
Selina Garcia ◽  
Jennifer Vanderhoof ◽  
Joshua Sifuentes ◽  
Yekaterina Brandt ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Mammary Gland Development ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Mouse Mammary Gland ◽  
Gland Development

scholarly journals Mammary gland development: cell fate specification, stem cells and the microenvironment

Development ◽  
2015 ◽  
Vol 142 (6) ◽  
pp. 1028-1042 ◽  
Author(s):  
J. L. Inman ◽  
C. Robertson ◽  
J. D. Mott ◽  
M. J. Bissell
Keyword(s):  
Stem Cells ◽  
Mammary Gland ◽  
Cell Fate ◽  
Mammary Gland Development ◽  
Cell Fate Specification ◽  
Fate Specification ◽  
Gland Development

scholarly journals Delayed Mammary Gland Involution in Mice with Mutation of the Insulin-Like Growth Factor Binding Protein 5 Gene

Endocrinology ◽  
2007 ◽  
Vol 148 (5) ◽  
pp. 2138-2147 ◽  
Author(s):  
Yun Ning ◽  
Bao Hoang ◽  
Alwin G. P. Schuller ◽  
Tara P. Cominski ◽  
Ming-Sing Hsu ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Mammary Gland Development ◽  
Binding Protein ◽  
Whole Body ◽  
Normal Mammary Gland ◽  
Igf Binding Proteins ◽  
Essentially Normal ◽  
Mammary Gland Involution ◽  
Igf I ◽  
Gland Development

IGFs (IGF-I and IGF-II) are essential for development, and their bioactivities are tightly regulated by six related IGF-binding proteins (IGFBPs). IGFBP-5 is the most highly conserved binding protein and is expressed in several key developmental lineages as well as in multiple adult tissues including the mammary gland. To explore IGFBP-5 actions in vivo, we produced IGFBP-5 knockout (KO) mice. Whole-body growth, selected organ weights, and body composition were essentially normal in IGFBP-5 KO mice, presumably because of substantial compensation by remaining IGFBP family members. The IGFBP-5 KO mice also exhibited normal mammary gland development and were capable of nursing their pups. We then directly evaluated the proposed role of IGFBP-5 in apoptosis and remodeling of mammary gland during involution. We found that the process of involution after forced weaning was delayed in IGFBP-5 KO mice, with both the appearance of apoptotic cells and the reappearance of adipocytes retarded in mutant mice, compared with controls. We also determined the effects of IGFBP-5 deletion on mammary gland development in pubertal females after ovariectomy and stimulation with estradiol/progesterone. In this paradigm, IGFBP-5 KO mammary glands exhibited enhanced alveolar bud formation consistent with enhanced IGF-I action. These results demonstrate that IGFBP-5, although not essential for normal growth, is required for normal mammary gland involution and can regulate mammary gland morphogenesis in response to hormone stimulation.

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