scholarly journals Cyclic growth of dermal papilla and regeneration of follicular mesenchymal components during feather cycling

Development ◽  
2021 ◽  
Author(s):  
Ping Wu ◽  
Ting-Xin Jiang ◽  
Mingxing Lei ◽  
Chih-Kuan Chen ◽  
Shu-Man Hsieh Li ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Growth Phase ◽  
Dermal Papilla ◽  
Tissue Homeostasis ◽  
Marker Genes ◽  
Molecular Signaling ◽  
Mesenchymal Progenitors ◽  
Interactive Interface ◽  
Dermal Cell

How dermis maintains tissue homeostasis in cyclic growth and wounding is a fundamental un-solved question. Here we study how dermal components of feather follicles undergo physiological (molting) and plucking injury-induced regeneration. Proliferation analyses reveal quiescent, transient-amplifying, and long-term label-retaining dermal cell (LRDC) states. In Growth phase, LRDCs are activated to make new dermal components with distinct cellular flows. Dermal transient amplifying (TA) cells, enriched in the proximal follicle, generate (i) peripheral pulp which extends distally to expand the epithelial-mesenchymal interactive interface for barb patterning, and (ii) central pulp which provides nutrition. Entering Resting phase, LRDCs, accompanying collar bulge epidermal LRC cells, descend to the apical dermal papilla. In the next cycle, these apical derma papilla LRDCs are re-activated to become new pulp progenitor TA cells. In growth phase, lower dermal sheath can generate dermal papilla and pulp. Transcriptome analyses identify marker genes and highlight molecular signaling associated with dermal specification. We compare cyclic topological changes with that of hair follicle, a convergently evolved follicle configuration. The work presents a model for analyzing homeostasis and tissue remodeling of mesenchymal progenitors.

scholarly journals Assessment of Prolonged Dengue Virus Infection in Dermal Fibroblasts and Hair-Follicle Dermal Papilla Cells

Viruses ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 267
Author(s):  
Kai-Che Wei ◽  
Wan-Ju Wei ◽  
Yi-Shan Liu ◽  
Li-Chen Yen ◽  
Tsung-Hsien Chang
Keyword(s):  
Dengue Virus ◽  
Hair Follicle ◽  
Hair Loss ◽  
Human Hair ◽  
Dermal Papilla ◽  
Denv Infection ◽  
Dermal Fibroblasts ◽  
Type I ◽  
Dermal Papilla Cells

Dengue virus (DENV)-mediated hair loss is one of the post-dengue fatigue syndromes and its pathophysiology remains unknown. Whether long-term or persistent infection with DENV in the scalp results in hair loss is unclear. In this study, we cultured human dermal fibroblasts (WS1 cells) and primary human hair-follicle dermal papilla cells (HFDPCs) in the long term with DENV-2 infection. The production of virion, the expression of inflammatory and anti-virus genes, and their signaling transduction activity in the infected cells were analyzed. DENV-2 NS3 protein and DENV-2 5′ UTR RNA were detected in fibroblasts and HFDPCs that were subjected to long-term infection with DENV-2 for 33 days. A significant amount of DENV-2 virion was produced by both WS1 cells and HFDPCs in the first two days of acute infection. The virion was also detected in WS1 cells that were infected in the long term, but HFDPCs failed to produce DENV-2 after long-term culture. Type I and type III interferons, and inflammatory cytokines were highly expressed in the acute phase of DENV infection in HFPDC and WS1 cells. However, in the long-term cultured cells, modest levels of anti-viral protein genes were expressed and we observed reduced signaling activity, which was correlated with the level of virus production changes. Long-term infection of DENV-2 downregulated the expression of hair growth regulatory factors, such as Rip1, Wnt1, and Wnt4. This in vitro study shows that the long-term infection with DENV-2 in dermal fibroblasts and dermal papilla cells may be involved with the prolonged-DENV-infection-mediated hair loss of post-dengue fatigue syndrome. However, direct evidence for viral replication in the human hair of a dengue victim or animal infection model is required.

scholarly journals Gene Expression Analysis of Chondrogenic Markers in Hair Follicle Dermal Papillae Cells Under the Effect of Laser Photobiomodulation and the Synovial Fluid

2019 ◽  
Vol 10 (3) ◽  
pp. 171-178
Author(s):  
Shirin Farivar ◽  
Roya Ramezankhani ◽  
Ezedin Mohajerani ◽  
Mohammad Hosein Ghazimoradi ◽  
Reza Shiari
Keyword(s):  
Gene Expression ◽  
Synovial Fluid ◽  
Hair Follicle ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Morphological Changes ◽  
Dermal Papilla ◽  
Marker Genes ◽  
Dermal Papilla Cells ◽  
Cell Groups

Introduction: Regarding the limited ability of the damaged cartilage cells to self-renew, which is due to their specific tissue structure, subtle damages can usually cause diseases such as osteoarthritis. In this work, using laser photobiomodulation and an interesting source of growth factors cocktail called the synovial fluid, we analyzed the chondrogenic marker genes in treated hair follicle dermal papilla cells as an accessible source of cells with relatively high differentiation potential. Methods: Dermal papilla cells were isolated from rat whisker hair follicle (Rattus norvegicus) and established cell cultures were treated with a laser (gallium aluminum arsenide diode Laser (λ=780 nm, 30 mW) at 5 J/cm2 ), the synovial fluid, and a combination of both. After 1, 4, 7, and 14 days, the morphological changes were evaluated and the expression levels of four chondrocyte marker genes (Col2a1, Sox-9, Col10a1, and Runx-2) were assessed by the quantitative real-time polymerase chain reaction. Results: It was monitored that treating cells with laser irradiation can accelerate the rate of proliferation of cells. The morphology of the cells treated with the synovial fluid altered considerably as in the fourth day they surprisingly looked like cultured articular chondrocytes. The gene expression analysis showed that all genes were up-regulated until the day 14 following the treatments although not equally in all the cell groups. Moreover, the cell groups treated with both irradiation and the synovial fluid had a significantly augmented expression in gene markers. Conclusion: Based on the gene expression levels and the morphological changes, we concluded that the synovial fluid can have the potential to make the dermal papilla cells to most likely mimic the chondrogenic and/or osteogenic differentiation, although this process seems to be augmented by the irradiation of the low-level laser.

scholarly journals STAT5 Activation in the Dermal Papilla Is Important for Hair Follicle Growth Phase Induction

2016 ◽  
Vol 136 (9) ◽  
pp. 1781-1791 ◽  
Author(s):  
Julien M.D. Legrand ◽  
Edwige Roy ◽  
Jonathan J. Ellis ◽  
Mathias Francois ◽  
Andrew J. Brooks ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Growth Phase ◽  
Dermal Papilla ◽  
Follicle Growth ◽  
Hair Follicle Growth

scholarly journals 219 STAT5 activation in the dermal papilla is important for hair follicle growth phase induction, hair follicle regeneration and wound healing

2016 ◽  
Vol 136 (9) ◽  
pp. S198 ◽  
Author(s):  
J. Legrand ◽  
R. Villani ◽  
E. Roy ◽  
K. Khosrotehrani
Keyword(s):  
Wound Healing ◽  
Hair Follicle ◽  
Growth Phase ◽  
Dermal Papilla ◽  
Follicle Growth ◽  
Hair Follicle Growth

scholarly journals Investigating PEGDA and GelMA Microgel Models for Sustained 3D Heterotypic Dermal Papilla and Keratinocyte Co-Cultures

2021 ◽  
Vol 22 (4) ◽  
pp. 2143 ◽  
Author(s):  
Justin J.Y. Tan ◽  
Duc-Viet Nguyen ◽  
John E. Common ◽  
Chunyong Wu ◽  
Paul C.L. Ho ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Three Dimensional ◽  
Dermal Papilla ◽  
Glycol Diacrylate ◽  
Prolonged Duration ◽  
Culture Models ◽  
Poly Ethylene

Hair follicle morphogenesis is heavily dependent on reciprocal, sequential, and epithelial-mesenchymal interaction (EMI) between epidermal stem cells and the specialized cells of the underlying mesenchyme, which aggregate to form the dermal condensate (DC) and will later become the dermal papilla (DP). Similar models were developed with a co-culture of keratinocytes and DP cells. Previous studies have demonstrated that co-culture with keratinocytes maintains the in vivo characteristics of the DP. However, it is often challenging to develop three-dimensional (3D) DP and keratinocyte co-culture models for long term in vitro studies, due to the poor intercellular adherence between keratinocytes. Keratinocytes exhibit exfoliative behavior, and the integrity of the DP and keratinocyte co-cultured spheroids cannot be maintained over prolonged culture. Short durations of culture are unable to sufficiently allow the differentiation and re-programming of the keratinocytes into hair follicular fate by the DP. In this study, we explored a microgel array approach fabricated with two different hydrogel systems. Using poly (ethylene glycol) diacrylate (PEGDA) and gelatin methacrylate (GelMA), we compare their effects on maintaining the integrity of the cultures and their expression of important genes responsible for hair follicle morphogenesis, namely Wnt10A, Wnt10B, and Shh, over prolonged duration. We discovered that low attachment surfaces such as PEGDA result in the exfoliation of keratinocytes and were not suitable for long-term culture. GelMA, on the hand, was able to sustain the integrity of co-cultures and showed higher expression of the morphogens overtime.

scholarly journals THE HUMAN HAIR FOLLICLE AS SENTINEL FOR DRUGS EVALUATION: DEMONSTRATION OF TETRACYCLINE ADHESION TO HAIR FOLLICLE AS PROPOSED MECHANISM IN DYSFUNCTIONAL HAIR LOSS

2020 ◽  
Vol 8 (11) ◽  
pp. 324-332 ◽  
Author(s):  
Abraham A. Embi
Keyword(s):  
Hair Follicle ◽  
Deleterious Effect ◽  
Dermal Papilla ◽  
The Elderly ◽  
Healthy Human ◽  
Exogenous Substance ◽  
Published Report

One mechanism of action of antibiotics such as tetracyclines involves the disruption of pathogens cell membranes. This author had previously demonstrated in vitro and in vivo the utility of a human miniorgan, a.k.a. hair follicle as sentinel in demonstrating the deleterious effect of alcohol by showing a disruption in metabolism. In this manuscript, the hair follicle was again used in vitro as sentinel in direct contact with another exogenous substance in two forms, namely liquid and powder tetracycline. The results demonstrate the adhesion property of tetracycline as a mechanism causing deleterious effect on the biological active cells of the follicle’s dermal papilla, and the consequent disruption in metabolism. Notably, it was documented a strong affinity of the antibiotic to the keratin skeleton of the hair follicle. In a recent published report, the adverse effect of tetracycline induction on experimentally deficient mitochondrial DNA (mtDNA) mouse was reversed and documented 30 days after discontinuation of the tetracycline diet. The experiments herein presented correlate and confirm previous findings of long term exposure to tetracycline causing not only damage the pathogen; but also healthy human cells. Since mtDNA may play a role in aging and age-associated diseases: Beware of tetracycline therapy on the elderly.

scholarly journals Hair follicle dermal condensation forms via Fgf20 primed cell cycle exit, cell motility, and aggregation

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Leah C Biggs ◽  
Otto JM Mäkelä ◽  
Satu-Marja Myllymäki ◽  
Rishi Das Roy ◽  
Katja Närhi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Motility ◽  
Hair Follicle ◽  
De Novo ◽  
Cell Movement ◽  
Dermal Papilla ◽  
Marker Genes ◽  
Cell Cycle Exit ◽  
Cellular Mechanisms ◽  
Molecular Events

Mesenchymal condensation is a critical step in organogenesis, yet the underlying molecular and cellular mechanisms remain poorly understood. The hair follicle dermal condensate is the precursor to the permanent mesenchymal unit of the hair follicle, the dermal papilla, which regulates hair cycling throughout life and bears hair inductive potential. Dermal condensate morphogenesis depends on epithelial Fibroblast Growth Factor 20 (Fgf20). Here, we combine mouse models with 3D and 4D microscopy to demonstrate that dermal condensates form de novo and via directional migration. We identify cell cycle exit and cell shape changes as early hallmarks of dermal condensate morphogenesis and find that Fgf20 primes these cellular behaviors and enhances cell motility and condensation. RNAseq profiling of immediate Fgf20 targets revealed induction of a subset of dermal condensate marker genes. Collectively, these data indicate that dermal condensation occurs via directed cell movement and that Fgf20 orchestrates the early cellular and molecular events.

Long-Term Culture of Mouse Vibrissal Dermal Papilla Cells and De Novo Hair Follicle Induction

2007 ◽  
Vol 13 (5) ◽  
pp. 975-982 ◽  
Author(s):  
Aki Osada ◽  
Tokuro Iwabuchi ◽  
Jiro Kishimoto ◽  
Tatsuo S. Hamazaki ◽  
Hitoshi Okochi
Keyword(s):  
Hair Follicle ◽  
De Novo ◽  
Dermal Papilla ◽  
Dermal Papilla Cells ◽  
Long Term Culture
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