Ultrasensitive RNA biosensors for SARS-CoV-2 detection in a simple color and luminescence assay

SummaryA luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

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Chemosensitivity of normal human trophoblasts evaluated by a newly developed ATP-based luminescence assay

Anti-Cancer Drugs ◽

10.1097/00001813-200208000-00004 ◽

2002 ◽

Vol 13 (7) ◽

pp. 701-708 ◽

Cited By ~ 2

Author(s):

Christian M Kurbacher ◽

Jutta A Kurbacher ◽

Ian A Cree ◽

Eva Wardelmann ◽

Ursula Stier ◽

...

Keyword(s):

Luminescence Assay ◽

Normal Human

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A Luminescence-Based Human TRPV1 Assay System for Quantifying Pungency in Spicy Foods

Foods ◽

10.3390/foods10010151 ◽

2021 ◽

Vol 10 (1) ◽

pp. 151

Author(s):

Minami Matsuyama ◽

Yuko Terada ◽

Toyomi Yamazaki-Ito ◽

Keisuke Ito

Keyword(s):

Fluorescent Probe ◽

Evaluation System ◽

Food Samples ◽

Assay System ◽

Sensory Test ◽

Chemosensory Receptor ◽

Luminescence Assay ◽

Transient Receptor ◽

Sensory Tests ◽

Fluorescence Dyes

The quantitation of pungency is difficult to achieve using sensory tests because of persistence, accumulation, and desensitization to the perception of pungency. Transient receptor vanilloid 1 (TRPV1), which is a chemosensory receptor, plays a pivotal role in the perception of many pungent compounds, suggesting that the activity of this receptor might be useful as an index for pungency evaluation. Although Ca2+-sensitive fluorescence dyes are commonly used for measuring human TRPV1 (hTRPV1) activity, their application is limited, as foods often contain fluorescent substances that interfere with the fluorescent signals. This study aims to design a new pungency evaluation system using hTRPV1. Instead of employing a fluorescent probe as the Ca2+ indicator, this assay system uses the luminescent protein aequorin. The luminescence assay successfully evaluated the hTRPV1 activity in foods without purification, even for those containing fluorescent substances. The hTRPV1 activity in food samples correlated strongly with the pungency intensity obtained by the human sensory test. This luminescence-based hTRPV1 assay system will be a powerful tool for objectively quantifying the pungency of spicy foods in both laboratory and industrial settings.

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Ratiometric Iridium(III) Complex-Based Phosphorescent Chemodosimeter for Hg2+ Applicable in Time-Resolved Luminescence Assay and Live Cell Imaging

Analytical Chemistry ◽

10.1021/ac503878s ◽

2015 ◽

Vol 87 (6) ◽

pp. 3255-3262 ◽

Cited By ~ 29

Author(s):

Jiaxi Ru ◽

Xu Chen ◽

Liping Guan ◽

Xiaoliang Tang ◽

Chunming Wang ◽

...

Keyword(s):

Live Cell Imaging ◽

Cell Imaging ◽

Live Cell ◽

Time Resolved ◽

Luminescence Assay

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Pleiotropic Roles of NOTCH1 Signaling in the Loss of Maturational Arrest of Human Osteoarthritic Chondrocytes

International Journal of Molecular Sciences ◽

10.3390/ijms222112012 ◽

2021 ◽

Vol 22 (21) ◽

pp. 12012

Author(s):

Manuela Minguzzi ◽

Veronica Panichi ◽

Stefania D’Adamo ◽

Silvia Cetrullo ◽

Luca Cattini ◽

...

Keyword(s):

Notch Signaling ◽

Terminal Differentiation ◽

Loss Of Function ◽

Notch Receptors ◽

Osteoarthritic Chondrocytes ◽

Gene And Protein Expression ◽

Luminescence Assay ◽

Culture Models ◽

Oa Chondrocytes

Notch signaling has been identified as a critical regulator of cartilage development and homeostasis. Its pivotal role was established by both several joint specific Notch signaling loss of function mouse models and transient or sustained overexpression. NOTCH1 is the most abundantly expressed NOTCH receptors in normal cartilage and its expression increases in osteoarthritis (OA), when chondrocytes exit from their healthy “maturation arrested state” and resume their natural route of proliferation, hypertrophy, and terminal differentiation. The latter are hallmarks of OA that are easily evaluated in vitro in 2-D or 3-D culture models. The aim of our study was to investigate the effect of NOTCH1 knockdown on proliferation (cell count and Picogreen mediated DNA quantification), cell cycle (flow cytometry), hypertrophy (gene and protein expression of key markers such as RUNX2 and MMP-13), and terminal differentiation (viability measured in 3-D cultures by luminescence assay) of human OA chondrocytes. NOTCH1 silencing of OA chondrocytes yielded a healthier phenotype in both 2-D (reduced proliferation) and 3-D with evidence of decreased hypertrophy (reduced expression of RUNX2 and MMP-13) and terminal differentiation (increased viability). This demonstrates that NOTCH1 is a convenient therapeutic target to attenuate OA progression.

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Further evaluation of luminol-enhanced luminescence in the diagnosis of disorders of leukocyte oxidative metabolism: role of myeloperoxidase.

Clinical Chemistry ◽

10.1093/clinchem/29.3.513 ◽

1983 ◽

Vol 29 (3) ◽

pp. 513-515 ◽

Cited By ~ 7

Author(s):

M S Cohen ◽

P S Shirley ◽

L R DeChatelet

Keyword(s):

Chronic Granulomatous Disease ◽

Oxidative Metabolism ◽

Clinical Consequence ◽

Granulomatous Disease ◽

Myeloperoxidase Deficiency ◽

Luminescence Assay ◽

Diagnostic Confusion ◽

Enhanced Luminescence ◽

Hereditary Nature

Abstract Chemiluminescence can be used to identify defects in the oxidative metabolism of granulocytes. This procedure has recently been adopted for use with microliter quantities of whole blood, appropriate for prenatal or neonatal study. Although the contribution of myeloperoxidase to the chemiluminescence assay has been noted, the possible diagnostic confusion between chronic granulomatous disease of childhood (which is rare and severe) and myeloperoxidase deficiency (which is common and of little clinical consequence) has not been stressed. We report a father and his infant daughter whose cells emitted no light in the luminol-enhanced luminescence assay; both patients are totally peroxidase deficient. These results emphasize the hereditary nature of myeloperoxidase deficiency, and the possibility for erroneous diagnosis of chronic granulomatous disease of childhood based on the luminol-enhanced luminescence test.

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Effects of Labrador Tea, Peppermint, and Winter Savory Essential Oils on Fusobacterium nucleatum

Antibiotics ◽

10.3390/antibiotics9110794 ◽

2020 ◽

Vol 9 (11) ◽

pp. 794

Author(s):

Amel Ben Lagha ◽

Katy Vaillancourt ◽

Patricia Maquera Huacho ◽

Daniel Grenier

Keyword(s):

Essential Oils ◽

Fusobacterium Nucleatum ◽

Significant Loss ◽

Oral Keratinocytes ◽

Mentha X Piperita ◽

Luminescence Assay ◽

Labrador Tea ◽

Bad Breath ◽

Bacterial Cell Membrane ◽

Adenosine Triphosphate Production

Bad breath or halitosis is an oral condition caused by volatile sulfur compounds (VSC) produced by bacteria found in the dental and tongue biofilms. Fusobacterium nucleatum is a Gram-negative anaerobic bacterium that has been strongly associated with halitosis. In this study, essential oils (EO) from three plants, Labrador tea (Rhododendron groenlandicum [Oeder] Kron & Judd), peppermint (Mentha x piperita L.), and winter savory (Satureja montana L.), were investigated for their effects on growth, biofilm formation and killing, and VSC production by F. nucleatum. Moreover, their biocompatibility with oral keratinocytes was investigated. Using a broth microdilution assay, winter savory EO and to a lesser extent Labrador tea and peppermint EO showed antibacterial activity against F. nucleatum. A treatment of pre-formed biofilms of F. nucleatum with EO also significantly decreased bacterial viability as determined by a luminescence assay monitoring adenosine triphosphate production. The EO were found to permeabilize the bacterial cell membrane, suggesting that it represents the target of the tested EO. The three EO under investigation were able to dose-dependently reduce VSC production by F. nucleatum. Lastly, no significant loss of cell viability was observed when oral keratinocytes were treated with the EO at concentrations effective against F. nucleatum. This study supports the potential of Labrador tea, peppermint, and winter savory EO as promising agents to control halitosis and promote oral health.

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Cellular delivery of enzyme-loaded DNA origami

Chemical Communications ◽

10.1039/c6cc08197e ◽

2016 ◽

Vol 52 (98) ◽

pp. 14161-14164 ◽

Cited By ~ 44

Author(s):

Ari Ora ◽

Erika Järvihaavisto ◽

Hongbo Zhang ◽

Henni Auvinen ◽

Hélder A. Santos ◽

...

Keyword(s):

Dna Origami ◽

Cellular Delivery ◽

Luminescence Assay

Enzyme-loaded DNA origamis are transported into cells, and the activity of the delivered enzymes is determined via luminescence assay.

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A convenient luminescence assay of ferroportin internalization to study its interaction with hepcidin

FEBS Journal ◽

10.1111/febs.12192 ◽

2013 ◽

Vol 280 (8) ◽

pp. 1773-1781 ◽

Cited By ~ 17

Author(s):

Ge Song ◽

Qian Jiang ◽

Ting Xu ◽

Ya-Li Liu ◽

Zeng-Guang Xu ◽

...

Keyword(s):

Luminescence Assay

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Porphyromonas gingivalis infection promotes mitochondrial dysfunction through Drp1-dependent mitochondrial fission in endothelial cells

International Journal of Oral Science ◽

10.1038/s41368-021-00134-4 ◽

2021 ◽

Vol 13 (1) ◽

Author(s):

Tong Xu ◽

Qin Dong ◽

Yuxiao Luo ◽

Yanqing Liu ◽

Liang Gao ◽

...

Keyword(s):

Endothelial Cells ◽

Mitochondrial Dysfunction ◽

Porphyromonas Gingivalis ◽

Vascular Endothelial Cells ◽

Mitochondrial Fission ◽

Mitochondrial Morphology ◽

Mitochondrial Fragmentation ◽

Atp Production ◽

Luminescence Assay ◽

The Impact

AbstractPorphyromonas gingivalis (P. gingivalis), a key pathogen in periodontitis, has been shown to accelerate the progression of atherosclerosis (AS). However, the definite mechanisms remain elusive. Emerging evidence supports an association between mitochondrial dysfunction and AS. In our study, the impact of P. gingivalis on mitochondrial dysfunction and the potential mechanism were investigated. The mitochondrial morphology of EA.hy926 cells infected with P. gingivalis was assessed by transmission electron microscopy, mitochondrial staining, and quantitative analysis of the mitochondrial network. Fluorescence staining and flow cytometry analysis were performed to determine mitochondrial reactive oxygen species (mtROS) and mitochondrial membrane potential (MMP) levels. Cellular ATP production was examined by a luminescence assay kit. The expression of key fusion and fission proteins was evaluated by western blot and immunofluorescence. Mdivi-1, a specific Drp1 inhibitor, was used to elucidate the role of Drp1 in mitochondrial dysfunction. Our findings showed that P. gingivalis infection induced mitochondrial fragmentation, increased the mtROS levels, and decreased the MMP and ATP concentration in vascular endothelial cells. We observed upregulation of Drp1 (Ser616) phosphorylation and translocation of Drp1 to mitochondria. Mdivi-1 blocked the mitochondrial fragmentation and dysfunction induced by P. gingivalis. Collectively, these results revealed that P. gingivalis infection promoted mitochondrial fragmentation and dysfunction, which was dependent on Drp1. Mitochondrial dysfunction may represent the mechanism by which P. gingivalis exacerbates atherosclerotic lesions.

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