scholarly journals Effects of Labrador Tea, Peppermint, and Winter Savory Essential Oils on Fusobacterium nucleatum

Antibiotics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 794
Author(s):  
Amel Ben Lagha ◽  
Katy Vaillancourt ◽  
Patricia Maquera Huacho ◽  
Daniel Grenier
Keyword(s):  
Essential Oils ◽  
Fusobacterium Nucleatum ◽  
Significant Loss ◽  
Oral Keratinocytes ◽  
Mentha X Piperita ◽  
Luminescence Assay ◽  
Labrador Tea ◽  
Bad Breath ◽  
Bacterial Cell Membrane ◽  
Adenosine Triphosphate Production

Bad breath or halitosis is an oral condition caused by volatile sulfur compounds (VSC) produced by bacteria found in the dental and tongue biofilms. Fusobacterium nucleatum is a Gram-negative anaerobic bacterium that has been strongly associated with halitosis. In this study, essential oils (EO) from three plants, Labrador tea (Rhododendron groenlandicum [Oeder] Kron & Judd), peppermint (Mentha x piperita L.), and winter savory (Satureja montana L.), were investigated for their effects on growth, biofilm formation and killing, and VSC production by F. nucleatum. Moreover, their biocompatibility with oral keratinocytes was investigated. Using a broth microdilution assay, winter savory EO and to a lesser extent Labrador tea and peppermint EO showed antibacterial activity against F. nucleatum. A treatment of pre-formed biofilms of F. nucleatum with EO also significantly decreased bacterial viability as determined by a luminescence assay monitoring adenosine triphosphate production. The EO were found to permeabilize the bacterial cell membrane, suggesting that it represents the target of the tested EO. The three EO under investigation were able to dose-dependently reduce VSC production by F. nucleatum. Lastly, no significant loss of cell viability was observed when oral keratinocytes were treated with the EO at concentrations effective against F. nucleatum. This study supports the potential of Labrador tea, peppermint, and winter savory EO as promising agents to control halitosis and promote oral health.

scholarly journals Preventive Effects ofHouttuynia cordataExtract for Oral Infectious Diseases

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Yasuko Sekita ◽  
Keiji Murakami ◽  
Hiromichi Yumoto ◽  
Takashi Amoh ◽  
Natsumi Fujiwara ◽  
...  
Keyword(s):  
Infectious Diseases ◽  
Skin Diseases ◽  
Water Solution ◽  
Oral Care ◽  
Ethanol Extract ◽  
Fusobacterium Nucleatum ◽  
Oral Keratinocytes ◽  
Oral Epithelial Cells ◽  
Anti Inflammatory ◽  
Oral Epithelial

Houttuynia cordata(HC) (Saururaceae) has been used internally and externally as a traditional medicine and as an herbal tea for healthcare in Japan. Our recent survey showed that HC poultice (HCP) prepared from smothering fresh leaves of HC had been frequently used for the treatment of purulent skin diseases with high effectiveness. Our experimental study also demonstrated that ethanol extract of HCP (eHCP) has antibacterial, antibiofilm, and anti-inflammatory effects againstS. aureuswhich caused purulent skin diseases. In this study, we focused on novel effects of HCP against oral infectious diseases, such as periodontal disease and dental caries. We determined the antimicrobial and antibiofilm effects of water solution of HCP ethanol extract (wHCP) against important oral pathogens and investigated its cytotoxicity and anti-inflammatory effects on human oral epithelial cells. wHCP had moderate antimicrobial effects against some oral microorganisms and profound antibiofilm effects againstFusobacterium nucleatum,Streptococcus mutans, andCandida albicans. In addition, wHCP had no cytotoxic effects and could inhibit interleukin-8 and CCL20 productions byPorphyromonas gingivalislipopolysaccharide-stimulated human oral keratinocytes. Our findings suggested that wHCP may be clinically useful for preventing oral infectious diseases as a mouthwash for oral care.

scholarly journals Synergistic Activity of New Diclofenac and Essential Oils Combinations against Different Candida spp.

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 688
Author(s):  
Antonio Rosato ◽  
Elisabetta Altini ◽  
Sabina Sblano ◽  
Lara Salvagno ◽  
Filippo Maggi ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oils ◽  
Diclofenac Sodium ◽  
Synergistic Activity ◽  
Candida Spp ◽  
Pelargonium Graveolens ◽  
Mentha X Piperita ◽  
Inflammatory Drugs ◽  
Antimicrobial And Antifungal Activity

According to recent studies, Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) have shown a good antimicrobial and antifungal activity. Their association with essential oils (EOs) could be useful for the treatment of infections caused by Candida spp. The aim of this studyis to evaluate the synergistic antifungal activity of new combinations between Diclofenac Sodium Salt (DSS), a widely used NSAID, with EOs of Mentha x piperita, Pelargonium graveolens and Melaleuca alternifolia. The in-vitroantifungal activity was determined on different Candida strains. The determination of the chemical composition of EOs was carried out by gaschromatography-massspectrometry (GC-MS). Susceptibility testing of planktonic cells was performed by using the broth microdilution assay and checkerboard methods. Minimum Inhibitory Concentrations (MIC) of DSS was in a range from 1.02 to 2.05 mg/mL reaching a MIC value of 0.05 mg/mL when combined with Pelargonium graveolens (FICI= 0.23–0.35) or Menthapiperita (FICI= 0.22–0.30) EOs. These preliminary results show thatthe combination of the EOs with DSS improves the antifungal activity on all the tested Candida strains.

scholarly journals Essential Oils and Supercritical CO2 Extracts of Arctic Angelica (Angelica archangelica L.), Marsh Labrador Tea (Rhododendron tomentosum) and Common Tansy (Tanacetum vulgare)—Chemical Compositions and Antimicrobial Activities

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7121
Author(s):  
Risto I. Korpinen ◽  
Anna-Liisa Välimaa ◽  
Jaana Liimatainen ◽  
Susan Kunnas
Keyword(s):  
Essential Oils ◽  
Antimicrobial Properties ◽  
Extraction Methods ◽  
Antimicrobial Activities ◽  
Chemical Compositions ◽  
Tanacetum Vulgare ◽  
Ledum Palustre ◽  
Angelica Archangelica ◽  
Labrador Tea ◽  
Rhododendron Tomentosum

Traditionally, arctic Finnish Angelica (Angelica archangelica L.), marsh Labrador tea (Rhododendron tomentosum, syn. Ledum palustre) and common tansy (Tanacetum vulgare) have been used as medicinal herbs in folklore medicine. However, these underutilised plants are a source of, e.g., oil-based compounds, which could benefit many modern applications implemented by the green chemistry extraction methods, as well. We extracted Angelica, marsh Labrador tea and common tansy by non-toxic and recyclable extraction methods, i.e., hydrodistillation and supercritical carbon dioxide (scCO2) extraction; characterised the essential oils (EOs) and scCO2 extracts by combination of gas chromatography and mass spectrometry (GC-MS), and in addition, analysed the antimicrobial properties. As expected for Angelica root and common tansy inflorescence, the scCO2 extraction method produced less amount of volatile compounds compared to hydrodistillation. On the other hand, more coumarins, alkanes, fatty alcohols and fatty acids were obtained. Additionally, sesquiterpenoids palustrol and ledol were predominant compounds in both marsh Labrador tea EO and scCO2 extract. According to our results, however, all the EOs and scCO2 extracts showed broad spectrum of antimicrobial activities against the selected microbes, but the effects were extract-specific. The strongest and broadest antimicrobial activities were performed by marsh Labrador tea scCO2 extract, which showed extremely strong effect on Staphylococcusaureus subsp. aureus and strong effect on Candida albicans.

scholarly journals The application of tea tree essential oil in dentistry

2007 ◽  
Vol 54 (2) ◽  
pp. 106-114
Author(s):  
D. Markovic ◽  
B. Mirkovic ◽  
T. Jovanovic ◽  
A. Knezevic ◽  
T. Nastovski
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Fusobacterium Nucleatum ◽  
Viral Diseases ◽  
Prevotella Intermedia ◽  
Tea Tree Oil ◽  
Melaleuca Alternifolia ◽  
Tea Tree ◽  
Plant Substances

Essential oils are widely used in medicine, dentistry and cosmetology as flavour and odour corrigents in various substances for oral hygiene. The aim of this study was to present comprehensively the possibilities for application of Melaleuca Alternifolia essential oil in dentistry based on the analysis of contemporary scientific and professional publications. The application of Tea tree essential oil in the treatment of periodontal, fungal and viral diseases is very efficient. The study of antimicrobial potential of ten different essential oils confirmed the efficiency of Tea tree oil against numerous Gram+ and Gram- bacteria. In vitro studies of bacteriostatic, bactericidal and fungicidal effect of Tea tree oil solution against ten different microorganisms confirmed sensitivity of the following microorganisms: Actinobacillus actinomycetemcomitans, Fusobacterium nucleatum and Porphyromonas gingivalis, and slightly weaker effect against Streptococcus Mutans and Prevotella intermedia. Tea tree is very effective in the treatment of various diseases and is an introduction and momentum for the application of plant substances in the treatment of numerous diseases in dentistry. .

scholarly journals Effect of Heat Stress on Yield, Monoterpene Content and Antibacterial Activity of Essential Oils of Mentha x piperita var. Mitcham and Mentha arvensis var. piperascens

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1903 ◽  
Author(s):  
Milad Heydari ◽  
Anna Zanfardino ◽  
Alireza Taleei ◽  
Ali Akbar Shahnejat Bushehri ◽  
Javad Hadian ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Heat Stress ◽  
Essential Oils ◽  
Mentha Arvensis ◽  
Electron Transmission ◽  
Mentha X Piperita ◽  
Induced Stress ◽  
Japanese Mint ◽  
Growth Chambers ◽  
Shed Light

Heat stress affects the yield of medicinal plants and can reduce biomass and/or metabolite production. In order to evaluate the effect of heat-induced stress on the essential oil production in Mentha x piperita L. var. Mitcham (Mitcham mint) and Mentha arvensis var. piperascens Malinv. ex L. H. Bailey (Japanese mint), we studied the chemical composition of the oils of the two mint species under different heat shock stresses in growth chambers. The antibacterial activity of the essential oils was also evaluated; microscopic observation (fluorescence and electron transmission) was used to assess the effect of the tested samples on bacterial growth. The results obtained shed light on the mint essential oils composition and biological activity in relation to heat stress.

scholarly journals B-Cell Deficiency Predisposes Mice to Disseminating Anaerobic Infections: Protection by Passive Antibody Transfer

2000 ◽  
Vol 68 (10) ◽  
pp. 5645-5651 ◽  
Author(s):  
Linda Hou ◽  
Hajime Sasakj ◽  
Philip Stashenko
Keyword(s):  
T Cell ◽  
B Cell ◽  
Adaptive Immune Response ◽  
Fusobacterium Nucleatum ◽  
Significant Loss ◽  
Genetically Engineered ◽  
Immunodeficient Mice ◽  
Systemic Spread ◽  
Anaerobic Infections ◽  
Deficient Mice

ABSTRACT We have previously demonstrated that a high proportion of RAG-2 SCID knockout mice, which lack T and B cells, develop orofacial abscesses and disseminated infections following pulpal infection, whereas immunocompetent control mice do not. In the present study, we sought to identify the components of the adaptive immune response which contribute to protection against disseminating anaerobic infections and sepsis. For this purpose, various genetically engineered immunodeficient mice were employed, including RAG-2 SCID, Igh-6 (B-cell deficient), Tcrb Tcrd (T-cell deficient) and Hc0 (C5 deficient). For abscess induction, the mandibular first molars were subjected to pulp exposure on day 0. Teeth were infected with a mixture of four anaerobic pathogens, including Prevotella intermedia, Streptococcus intermedius,Fusobacterium nucleatum, and Peptostreptococcus micros, and teeth were sealed to prevent communication with the oral cavity. The findings demonstrate that both RAG-2 SCID and B-cell-deficient mice, but not T-cell- or C5-deficient mice, have increased susceptibility to the development of disseminating anaerobic infections. Abscess-susceptible RAG-2 SCID and B-cell-deficient mice also showed a significant loss of body weight, splenomegaly, and absent antibacterial antibody production. Furthermore, dissemination was significantly reduced, from 74 to 25%, in susceptible RAG-2 mice by passively transferred antibody, predominantly immunoglobulin G2b (IgG2b) and IgM, against the infecting bacterial innoculum. Fractionated IgG-enriched preparations were more efficient in transferring protection than IgM preparations. We conclude that an antibody-mediated mechanism(s), most likely bacterial opsonization, is of importance in localizing anaerobic root canal infections and in preventing their systemic spread.

scholarly journals Antioxidant properties of essential oils from Mentha species evidenced by electrochemical methods

2009 ◽  
Vol 11 (4) ◽  
pp. 372-382 ◽  
Author(s):  
R.S. Gonçalves ◽  
A. Battistin ◽  
G. Pauletti ◽  
L. Rota ◽  
L.A. Serafini
Keyword(s):  
Essential Oils ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Differential Pulse ◽  
Limiting Current ◽  
Mentha Piperita ◽  
Mentha Spicata ◽  
Active Compounds ◽  
K Value ◽  
Mentha X Piperita

The antioxidant capacity of essential oils of Mentha spicata L., Mentha x gentilis L., Mentha crispa L., Mentha piperita L. and Mentha x piperita L. was determined by using differential pulse voltammetry. This assay was based on the reduction in the limiting current value of the oxygen electroreduction, which was used to calculate the antioxidant capacity (K) of these species. The species M. x gentilis L. had the highest K value. Cyclic voltammetry experiments confirmed the interaction between the electrode surface and the active compounds present in the essential oils. A simple electrochemical method for determining the ability of active compounds to chelate Fe (II) is proposed.

scholarly journals Composition and Antimicrobial Activities of Lippia multiflora Moldenke, Mentha x piperita L. and Ocimum basilicum L. Essential Oils and Their Major Monoterpene Alcohols Alone and in Combination

Molecules ◽  
2010 ◽  
Vol 15 (11) ◽  
pp. 7825-7839 ◽  
Author(s):  
Imaël Henri Nestor Bassolé ◽  
Aline Lamien-Meda ◽  
Balé Bayala ◽  
Souleymane Tirogo ◽  
Chlodwig Franz ◽  
...  
Keyword(s):  
Essential Oils ◽  
Antimicrobial Activities ◽  
Ocimum Basilicum ◽  
Lippia Multiflora ◽  
Mentha X Piperita

scholarly journals Effect of Silver Nanoparticles on Blue Light Phototoxicity against Fusobacterium nucleatum

Biophysica ◽  
2021 ◽  
Vol 1 (4) ◽  
pp. 405-412
Author(s):  
Uziel Jeffet ◽  
Shiri Livne ◽  
Arkadi Rahmanov ◽  
Nir Sterer
Keyword(s):  
Silver Nanoparticles ◽  
Cell Membrane ◽  
Blue Light ◽  
Bacterial Cell ◽  
Light Exposure ◽  
Membrane Damage ◽  
Fusobacterium Nucleatum ◽  
Oxygen Species ◽  
Cell Membrane Damage ◽  
Bacterial Cell Membrane

A previous study showed that sub-lethal exposure of blue light caused cell membrane damage in Fusobacterium nucleatum (Fn). The aim of the present study was to test the combined effect of blue light and silver nanoparticles against Fn. Bacterial suspensions were exposed to blue light (400–500 nm) with or without silver nanoparticles (10 nm). Exposed and non-exposed samples were studied for malodor production (Odor judge scores), VSC levels (Halimeter), reactive oxygen species (ROS) production (fluorimeter), and bacterial cell membrane damage (fluorescence microscopy). The results showed that combining blue light exposure and silver nanoparticles significantly reduced malodor and VSC production by Fn concomitant with increased ROS levels and bacterial cell membrane damage. These results suggest that silver nanoparticles may increase blue light phototoxicity against Fn.

Sign in / Sign up

Export Citation Format

Share Document