Characterization of CTX-M-15-Klebsiella pneumoniae from inpatients and outpatients of a teaching hospital

Background The presence of Extended-spectrum β-lactamase (ESBL) positive bacteria in hospital setting is an aggravating influential factor for hospitalized patients, and its consequences may be hazardous. Therefore, there is a need for rapid detection methods for newly emerging drug-resistant bacteria. This study was aimed at the molecular characterization of ESBL-positive Klebsiella pneumoniae isolates recovered from clinical samples. Methods A total of 513 K. pneumoniae isolates were obtained from various clinical samples during June 2019 to May 2020. The collected isolates were investigated for antimicrobial susceptibility (antibiogram), and PCR and DNA sequencing were performed to analyse the ESBL genes. Results Among the 513 isolates, as many as 359 (69.9%) were ESBL producers and 87.5% were multi-drug resistant, while none had resistance to imipenem. PCR scored 3% blaTEM, 3% blaSHV, and 60% blaCTX-M-15 genes for the tested isolates. Conclusion The study showed that CTX-M-15 was the major prevalent ESBL type among the isolates. Additionally, all the isolates were susceptible to carbapenems. Screening and detection of ESBL tests are necessary among all isolates from the enterobacteriaceae family in routine microbiology laboratory to prevent associated nosocomial infections. A larger study is essential to understand molecular epidemiology of ESBL producing organisms to minimize morbidities due to these multidrug resistant organisms.

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Molecular Epidemiological Surveillance of CTX-M-15-producing Klebsiella pneumoniae from the patients of a teaching hospital in Sindh, Pakistan.

F1000Research ◽

10.12688/f1000research.53221.3 ◽

2021 ◽

Vol 10 ◽

pp. 444

Author(s):

Muzaheed Muzaheed ◽

Naveed Sattar Shaikh ◽

Saeed Sattar Shaikh ◽

Sadananda Acharya ◽

Shajiya Sarwar Moosa ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Teaching Hospital ◽

Hospital Setting ◽

Influential Factor ◽

Detection Methods ◽

Epidemiological Surveillance ◽

Clinical Samples ◽

Resistant Bacteria ◽

Drug Resistant ◽

Extended Spectrum

Background The presence of Extended-spectrum β-lactamase positive bacteria in hospital setting is an aggravating influential factor for hospitalized patients, and its consequences may be hazardous. Therefore, there is a need for rapid detection methods for newly emerging drug-resistant bacteria. This study was aimed at the molecular characterization of Extended-spectrum β-lactamase -positive Klebsiella pneumoniae isolates recovered from the patients of a teaching hospital in Sindh, Pakistan. Methods A total of 513 K. pneumoniae isolates were obtained from various clinical samples during June 2019 to May 2020. The collected isolates were investigated for antimicrobial susceptibility (antibiogram), and PCR and DNA sequencing were performed to analyse the ESBL genes. Results Among the 513 isolates, as many as 359 (69.9%) were Extended-spectrum β-lactamase producers and 87.5% were multi-drug resistant, while none had resistance to imipenem. PCR scored 3% blaTEM, 3% blaSHV, and 60% blaCTX-M-15 genes for the tested isolates. Conclusion The study showed that CTX-M-15 was the major prevalent Extended-spectrum β-lactamase type among the isolates. Additionally, all the isolates were susceptible to carbapenems. Screening and detection of Extended-spectrum β-lactamase tests are necessary among all isolates from the enterobacteriaceae family in routine microbiology laboratory to prevent associated nosocomial infections. A larger study is essential to understand molecular epidemiology of Extended-spectrum β-lactamase producing organisms to minimize morbidities due to these multidrug resistant organisms.

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Synergistic antibacterial effects of colistin in combination with aminoglycoside, carbapenems, cephalosporins, fluoroquinolones, tetracyclines, fosfomycin, and piperacillin on multidrug resistant Klebsiella pneumoniae isolates

PLoS ONE ◽

10.1371/journal.pone.0244673 ◽

2021 ◽

Vol 16 (1) ◽

pp. e0244673

Author(s):

Julalak C. Ontong ◽

Nwabor F. Ozioma ◽

Supayang P. Voravuthikunchai ◽

Sarunyou Chusri

Keyword(s):

Antibacterial Activity ◽

Klebsiella Pneumoniae ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Drug Resistant ◽

Antibacterial Effects ◽

Drug Resistant Bacteria ◽

Multidrug Resistant Pathogens ◽

Conventional Antibiotics ◽

Global Health Problem

Multidrug resistant Enterobacterales have become a serious global health problem, with extended hospital stay and increased mortality. Antibiotic monotherapy has been reported ineffective against most drug resistant bacteria including Klebsiella pneumoniae, thus encouraging the use of multidrug therapies as an alternative antibacterial strategy. The present works assessed the antibacterial activity of colistin against K. pneumoniae isolates. Resistant isolates were tested against 16 conventional antibiotics alone and in combination with colistin. The results revealed that all colistin resistant isolates demonstrated multidrug resistance against the tested antibiotics except amikacin. At sub-inhibitory concentrations, combinations of colistin with amikacin, or fosfomycin showed synergism against 72.72% (8 of 11 isolates). Colistin with either of gentamicin, meropenem, cefoperazone, cefotaxime, ceftazidime, moxifloxacin, minocycline, or piperacillin exhibited synergism against 81.82% (9 of 11 isolates). Combinations of colistin with either of tobramycin or ciprofloxacin showed synergism against 45.45% (5 in 11 isolates), while combinations of colistin with imipenem or ceftolozane and tazobactam displayed 36.36% (4 of 11 isolates) and 63.64% (7 of 11 isolates) synergism. In addition, combinations of colistin with levofloxacin was synergistic against 90.91% (10 of 11 isolates). The results revealed that combinations of colistin with other antibiotics could effectively inhibit colistin resistant isolates of K. pneumoniae, and thus could be further explore for the treatment of multidrug resistant pathogens.

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Complete Genome Sequence of a Novel Multidrug-Resistant Klebsiella pneumoniae Phage, vB_Kpn_IME260

Genome Announcements ◽

10.1128/genomea.00055-17 ◽

2017 ◽

Vol 5 (19) ◽

Author(s):

Shaozhen Xing ◽

Xiangchun Pan ◽

Qiang Sun ◽

Guangqian Pei ◽

Xiaoping An ◽

...

Keyword(s):

Public Health ◽

Klebsiella Pneumoniae ◽

Phage Therapy ◽

Bacterial Pathogen ◽

Genomic Data ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Drug Resistant ◽

Content Type ◽

Drug Resistant Bacteria

ABSTRACT Klebsiella pneumoniae is the most common clinically important opportunistic bacterial pathogen and its infection is often iatrogenic. Its drug resistance poses a grave threat to public health. The genomic data reported here comprise an important resource for research on phage therapy in the control of drug-resistant bacteria.

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High frequency of Gram-negative drug-resistant bacteria both in community- and hospital-acquired bloodstream infections (BSI) in a hospital setting from northern Italy

10.26226/morressier.56d6be74d462b80296c974b5 ◽

2016 ◽

Author(s):

Ottavia Viganò

Keyword(s):

High Frequency ◽

Hospital Setting ◽

Bloodstream Infections ◽

Northern Italy ◽

Resistant Bacteria ◽

Drug Resistant ◽

Gram Negative ◽

Drug Resistant Bacteria ◽

Hospital Acquired

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Proposed amendments regarding the definitions of multidrug-resistant and extensively drug-resistant bacteria

Expert Review of Anti-infective Therapy ◽

10.1080/14787210.2021.1945922 ◽

2021 ◽

Author(s):

Petros I. Rafailidis ◽

Diamantis Kofteridis

Keyword(s):

Multidrug Resistant ◽

Resistant Bacteria ◽

Drug Resistant ◽

Extensively Drug Resistant ◽

Drug Resistant Bacteria

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Isolation and Characterization of Two Klebsiella pneumoniae Phages Encoding Divergent Depolymerases

International Journal of Molecular Sciences ◽

10.3390/ijms21093160 ◽

2020 ◽

Vol 21 (9) ◽

pp. 3160 ◽

Cited By ~ 1

Author(s):

Pilar Domingo-Calap ◽

Beatriz Beamud ◽

Lucas Mora-Quilis ◽

Fernando González-Candelas ◽

Rafael Sanjuán

Keyword(s):

Klebsiella Pneumoniae ◽

Multidrug Resistant ◽

Health Concern ◽

Resistant Bacteria ◽

Isolation And Characterization ◽

The Family ◽

Important Challenge ◽

Tail Spike ◽

Reference Strains

The emergence of multidrug-resistant bacteria is a major global health concern. The search for new therapies has brought bacteriophages into the spotlight, and new phages are being described as possible therapeutic agents. Among the bacteria that are most extensively resistant to current antibiotics is Klebsiella pneumoniae, whose hypervariable extracellular capsule makes treatment particularly difficult. Here, we describe two new K. pneumoniae phages, πVLC5 and πVLC6, isolated from environmental samples. These phages belong to the genus Drulisvirus within the family Podoviridae. Both phages encode a similar tail spike protein with putative depolymerase activity, which is shared among other related phages and probably determines their ability to specifically infect K. pneumoniae capsular types K22 and K37. In addition, we found that phage πVLC6 also infects capsular type K13 and is capable of striping the capsules of K. pneumoniae KL2 and KL3, although the phage was not infectious in these two strains. Genome sequence analysis suggested that the extended tropism of phage πVLC6 is conferred by a second, divergent depolymerase. Phage πVLC5 encodes yet another putative depolymerase, but we found no activity of this phage against capsular types other than K22 and K37, after testing a panel of 77 reference strains. Overall, our results confirm that most phages productively infected one or few Klebsiella capsular types. This constitutes an important challenge for clinical applications.

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Isolation of Multidrug Resistant Bacteria from Aspirates of Cancer Patients

Bangladesh Journal of Microbiology ◽

10.3329/bjm.v35i1.39805 ◽

2019 ◽

Vol 35 (1) ◽

pp. 61-66

Author(s):

Sunjukta Ahsan ◽

Rayhan Mahmud ◽

Kajal Ahsan ◽

Shamima Begum

Keyword(s):

Cancer Patients ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Gram Negative Bacteria ◽

Drug Resistant ◽

Multidrug Resistant Bacteria ◽

Esbl Producer ◽

Drug Resistant Bacteria ◽

Treatment Facilities ◽

Isolated Species

Infections due to Gram-negative bacteria are common affairs in cancer patients during aggressive therapy. The present study characterizedmulti-drug resistant bacteria (MDR) isolated from cancer aspirates collected from patients admitted to the National Cancer Hospital in Dhaka, Bangladesh. A total of 210 aspirate samples were collected from cancer patients. Out of 210 samples Acinetobacter spp.led the list of isolates (8.89%, n=45). Of these species, 50% exhibited resistance to Amoxycillin and Nitrofurantoin, each, 25% exhibited resistant to Cefotaxime, Azithromycin, Ciprofloxacin, Clindamycin, and Sulfamethoxazole. A total of 33.33% of the Bordetella spp.which accounted 6.67%of the total isolates exhibited resistance to Cefotaxime. All oftheLegionellapneumophila,comprising 4.4%of the isolated species, wereresistant to Cefotaxime, Azithromycin, and Clindamycin.In contrast, 50% were resistant to Cefotaxime, Azithromycin, and Ceftriaxone. Of the Escherichia coli(4.4%, n=45) isolated,50% exhibited resistance to Cefotaxime, Clindamycin, Ceftriaxone, Amoxycillinand Sulfamethoxazole.The only isolate of Klebsiella sp. was demonstrated to be an ESBL producer. The isolation of multidrug resistant bacteria from cancer patients is of particular concern in Bangladesh where cancer and drug resistance are both common phenomena but treatment facilities are poor. To our knowledge this is the first report of the isolation of drug resistant bacteria from cancer patients from Dhaka city. Bangladesh J Microbiol, Volume 35 Number 1 June 2018, pp 61-66

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