Identification and Evaluation of Preservative Plants on Traditionally Fermented Cow Milk

2021 ◽  
Vol 9 (1) ◽  
pp. 329-337
Author(s):  
Zerihun Shambel ◽  
Ewonetu Kebede ◽  
Melese Mengistu ◽  
Tesfahun Lamboro

The majority of communities in different regions of Ethiopia are relying on a variety of plants to improve the quality of their dairy products. However, this cultural perception was scientifically not well strengthened. Therefore, the objectives of the study were to identify milk preservative plants and evaluate the effects of preservative plants on fermented milk redox potential, Potential hydrogen (pH), and sensory analysis. The survey study was conducted on purposefully selected 80 households in the Haramaya district. However, the laboratory study was conducted on four top-ranked plants for the preparation of fermented milk samples at ambient temperature following similar techniques and procedures observed at households. All the collected data were analysed by Statitsical Analysis software (SAS). In the study area, five plants in the families of Oleaceae, Celastraceae, Lamiaceae, and Rutaceae were identified and used by the majority of respondents with perceptions of enhancing the flavour of their products. The Analysis of variance (ANOVA) results of redox potential were proved the cultural perception of the majority of respondents that they were used both Olea Africana and Catha edulis in substitute to each other for the same purpose. The pH of all treatments was continuously decreased and the milk samples treated by Olea Africana and Catha edulis were recorded the lowest values at the end. The observed pH results have disproved the communities cultural perceptions that they believed smoking increased the shelf life and extended the fermentation time whereas the Hedonic scores of panelists proved the local perceptions of respondents that they were mainly intended to make their products much more acceptable and preferable by its flavour to the consumers.

Author(s):  
I. Korniienko

The use of modern biotechnological approaches in the field of baking allows to improve the quality of finished products, reduce the duration of the technological cycle and increase the functional properties of bread. The aim of the study was to study the effect of ascorbic acid on the intensity of fermentation processes during dough maturation. The task of the work is to establish the optimal amount of improver — ascorbic acid, taking into account organoleptic, microbiological and structural-mechanical quality indicators. The intensity of microbiological and biochemical processes during dough maturation (fermentation) directly depends on the concentration of hydrogen ions compared to the total content of acid-reactive products, as well as redox potential, titrated acidity and titer of fermentation microflora.The introduction of ascorbic acid in the amount of 0.1—0.12 g/kg of flour, the fermentation time of the dough was reduced from 270 to 225 minutes, the speed and completeness of redox reactions (redox potential  -50 mV) were increased. redox potential reactions catalyzed by the enzymes peroxidases and catalases are associated with the oxidation of unsaturated fatty acids, which promote the action of lipoxygenase by disrupting its inhibitor - hydrogen peroxide. Experiments have confirmed the activation of the fermenting microflora of the dough and the increase of its titer. Indicators of active (pH 4.66—4.51) and titrated acidity (6.0—6.5 degrees Turner) are within the limits that fully meet the optimal conditions for the cultivation of lactic acid bacteria, which indicates the correct selection of the amount of ascorbic acid and the composition of the nutritious flour mixture.


2013 ◽  
Vol 29 (2) ◽  
pp. 90-95
Author(s):  
MM Rahman ◽  
MN Islam ◽  
NU Siddiquee ◽  
MA Habib

The keeping quality of raw cow milk at room temperatures was evaluated in different containers with or without sodium bicarbonate. Milk samples stored in containers of glass, tinned iron, plastic, aluminium, earthen and bronze at 12 to 20oC were analyzed every two hours for acidity and clot-on-boiling (COB) tests. Milk from the organised dairy farm kept well in aluminium containers up to ten hours, but milk from villages deteriorated after six hours. The keeping qualities of milk were poorer in other containers, and worst in earthen container. In COB test, milk from BAU dairy farm showed no changes for eight hours in all containers. Milk in aluminium container kept 14 hours, but only eight hours in earthen pot. Milk collected from villages kept for ten hours in aluminium container and six hours in earthen container. After mixing sodium bicarbonate 0.05 and 0.1%, milk kept well up to 16 hours, and up to 20 hours with 0.15% based on acidity test. Addition of Na2Co3 improved the keeping quality till 24 hours of storage at 10 – 19oC in aluminium container. DOI: http://dx.doi.org/10.3329/bvet.v29i2.14348 Bangl. vet. 2012. Vol. 29, No. 2, 90-95


Author(s):  
Tian Lv ◽  
Xueting Huang ◽  
Chenchen Zhang ◽  
Dawei Chen ◽  
Ruixia Gu ◽  
...  

Kefir is an acidic-alcoholic fermented milk that can provide probiotic benefits such as intestinal microecological balance regulation, antibacterial activity, and anti-inflammatory activity. In this study, Lactobacillus fermentum grx08 isolated from longevous people was used to further improve the health properties of kefir. L. fermentum grx08 and kefir grains obtained from Xinjiang, China were mixed at ratios of 1:1, 5:1 and 25:1 as starters. The 6 Gram-positive and Gram-negative foodborne pathogens were able to grow in the supernatant of kefir, but not in the supernatant of kefir with L. fermentum grx08. With the increasing of amount of inoculated L. fermentum grx08, the antibacterial activity of the mixed fermented kefir gradually increased. The contents of lactic acid, fumaric acid and malic acid in the mixed fermented milk were significantly increased by adding L. fermentum grx08 (P < 0.05), while the content of acetic acid decreased with the increase of L. fermentum grx08, and the content of citric acid was unaffected. This study suggest that the addition of  L. fermentum grx08 shortened the fermentation time, improved the acidity and retained the quality of fermented milk. Moreover, the antibacterial properties of kefir is enhanced by increasing the production of certain acid.


2011 ◽  
Vol 24 (No. 4) ◽  
pp. 164-171 ◽  
Author(s):  
H. Pavlović ◽  
J. Hardi ◽  
V. Slačanac ◽  
M. Halt ◽  
D. Kocevski

This study was performed to determine the influence of fermented goat and cow milk produced by the use of Bifidobacterium longum Bb-46 on pathogenic Serratia marcescens and Campylobacter jejuni strains. The correlation between the inhibitory effect and some fermentation parameters (the number of viable probiotic cells and pH of fermented milk) was also determined. Bifidobacterium longum counts and pH values were also measured in milk samples during fermentation. The results showed that the inhibitory effect of Bifidobacterium longum Bb-46 fermented goat milk on Serratia marcescens increased with the fermentation time. The highest inhibitory effect of fermented cow milk occurred in the middle course of fermentation. Statistically significant correlation between the inhibition degree of Serratia marcescens growth and pH values of fermented goat milk was noted as opposed to the correlation between the inhibition degree of Serratia marcescens growth and pH values of fermented cow milk which was not statistically significant. All samples of goat and cow fermented milk exhibited inhibitory effects on the growth of Campylobacter jejuni.  


Jurnal Biota ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 85-93
Author(s):  
Pratika Viogenta ◽  
Nani Kartinah ◽  
Amalia Khairunnisa ◽  
Fathur Rahman

One of the fermented milk products, namely kefir, is increasingly popular because it has many health benefits. Peanut juice has a high enough protein content that it can be used as a substitute for animal milk. The purpose of this study was to determine the quality of peanut kefir with variations in the concentration of ragi tape inoculum and fermentation time. This research method used 3 variations in the concentration of tape yeast (1, 2 and 4%) and long fermentation time (24, 48 and 72 hours). The results showed that the highest total LAB was in 4% ragi tape inoculum with a fermentation time of 24 hours (4.4x108 cells/mL) and the lowest was in 4% tape yeast inoculum with a fermentation time of 72 hours (9.8x107 cells/mL) and total yeast between 1x104 cells/mL - 3x105 cells/mL) and alcohol produced <1%. Total acid obtained between 6% - 17.6%. The increase in total acid is proportional to the decrease in pH. The pH of the peanut kefir medium was between 3.44 - 4.12. Peanut kefir with tape yeast inoculum meets the standard requirements for fermented milk and can replace milk kefir.


2018 ◽  
Vol 5 (1) ◽  
pp. 75-85
Author(s):  
Mohammad Mizanur Rahman ◽  
Md Nurul Islam ◽  
Md Harun Ur Rashid ◽  
AKM Masum

The present investigation was carried out to assess the effect of sodium bicarbonate (NaHCO3) on the keeping quality of raw cow milk. Milk samples were collected from Bangladesh Agricultural University (BAU) Dairy Farm and initial quality were evaluated using physical and chemical tests at Dairy Technology Laboratory of the Department of Dairy Science, BAU. Then, collected samples were preserved at room temperature (28- 30°C) with 0.0 (control), 0.3, 0.4 and 0.5 per cent NaCHO3. The quality of milk samples were measured at every two hours interval up to 6 hours, one hour interval upto 16 hours and thereafter every half an hour interval until spoilage. Initially, colour, flavour and texture of all milk samples were normal (100%), but with progressive storage time colour, flavour and texture of all samples deteriorated gradually, which was more rapid for control samples than that of the NaHCO3 treated samples. Acidity per cent of all samples increased gradually during storage period and the differences in acidity of milk samples in different treatments were significant (p<0.01). Control samples spoiled after 11¼th hours but that of 0.3, 0.4 and 0.5 percent NaHCO3 treated samples spoiled after 16¾th, 17¾th and 18¾th hours,, respectively. It may be concluded that NaHCO3 is the effective chemical neutralizing the acids produced by acid producing bacteria and can be used as a short term preservation of milk under rural condition of Bangladesh where scientific cooling or pasteurization facilities are not available.Res. Agric., Livest. Fish.5(1): 75-85, April 2018


2021 ◽  
Vol 9 (1) ◽  
pp. 111-121
Author(s):  
Isaac M. Maitha ◽  
Dasel W. M. Kaindi ◽  
Cherotich Cheruiyot

Fermentation serves a key role in inhibiting spoilage microorganism through acidification and production of antimicrobial compounds. The technological information on properties of Streptococcus infantarius sub sp. infantarius which is predominant in most African fermented is dairy products very little. This study was therefore carried out to determine the functionality of selected African Streptococci strains in fermented dairy products. Pasteurized milk samples from camels and cows were inoculated with different strains and a selected combination at a rate of 3% v/v and incubated at different temperatures of 25 oC, 30 oC, 37 oC, and 45 oC for 9 hours. Analysis was done after every 3 hours for pH and titratable acidity while viscosity was done after incubation and cooling of the product. The different fermented milk samples were subsequently evaluated for consumer acceptability. Milk inoculated with both African type Streptococcus thermophillus (146A8.2) and Streptococcus infantarius sub sp. infantarius CJ 18 (9377), and incubated for nine hours recorded the highest amount of titratable acidity of 0.97 for the camel milk and had the least pH value of 4.12 for cow milk compared to the other strains. The cow milk had the highest viscosity level of 59.64 cPs compared to camel milk which was 29.44 cPs. The levels of titratable acidity and viscosity depended on the strain and incubation temperature. The African type Streptococcus thermophillus (146A8.2) and Streptococcus infantarius sub sp. infantarius CJ 18 (9377), isolated from fermented camel milk had good technological properties that are useful as starter culture for development of fermented milk products.


2021 ◽  
Vol 2 (3) ◽  
pp. 64-72
Author(s):  
Asged, A. A. Mohammed ◽  
I. E. M. El Zubeir

In the present investigation, the chemical composition of milk was determined and the detection of the presence of some additives and preservatives in raw cow milk obtained from rural area of Omdurman city Sudan was done. The milk samples (n= 120) were obtained from rural areas of North and South Omdurman. Significantly, (P≤0.05) higher values for the chemical constituents of the examined raw milk samples were reported in the samples from South Omdurman compared to those from North Omdurman. In addition, the results showed that the added water was found in 16.7% of the examined milk samples. Also, formaldehyde was used as a preservative in 19.2% of the samples, while boric acid was used as preservative in 25% of the milk samples in South Omdurman. However, sodium bicarbonate, hydrogen peroxide and starch were not detected. It was concluded that milk distributed in North and South Omdurman contains some adulterants. Hence control and monitoring programs should be directed towards the hygienic quality of milk during production and marketing.


2018 ◽  
Vol 1 (32) ◽  
pp. 68-76
Author(s):  
Nguyen Bao Pham ◽  
Hoa Thai Ma ◽  
Duyen Thi Cam Pham

Yogurt is a food product fermented from milk by Streptococcus thermophilus (S. thermophilus) and Lactobacillus bulgaricus (L. bulgaricus), which is very good for health. This study focuses on the evaluation of the ratio of condensed milk / Non-sugar fresh milk from 1/1.5 - 1/2 - 1/2.5, the ratio of the puree of soursop pulp added (5-25%), the fermentation temperature (from 41 to 47 oC) and fermentation time (from 6 to 8 hours) on the fermentation ability of yogurt in order toidentify the suitable fermentation condition for producing yogurt with the best sensory quality and the suitable sour taste. Additionally, this study also  investigates the effect of temperature and time of the fermentation process on the quality of yogurt. The results showed that, with the ratio of condensed milk / Non-sugar fresh milk = 1/2, the sensory properties of yogurt were very good. Besides, with the added puree of 5% and fermentation temperature of 44 oC within 7 hours, the sensory quality of yogurt was the best (with the point of 4.7/5 by the quality description scale) and the Donic degree of the product reached 86.3 that was suitable for yogurt (70 to 90 oD)


2019 ◽  
Vol 7 (9) ◽  
pp. 274 ◽  
Author(s):  
Dimitra Dimitrellou ◽  
Chrysoula Salamoura ◽  
Artemis Kontogianni ◽  
Despoina Katsipi ◽  
Panagiotis Kandylis ◽  
...  

The production of fermented milk using cow milk, goat milk and a mixture of them (1:1) was evaluated. The traditional yogurt starter culture was used—Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus, along with Lactobacillus casei ATCC 393. The objective was to monitor the viability of these cultures during the storage of fermented milk at 4 °C for up to 28 days. Storage significantly affected the viability of all microorganisms and especially L. bulgaricus. All microorganisms retained viable numbers higher than 7.5 log CFU g−1, even after 4 weeks of storage, which is important to confirm the probiotic character of the product. The use of goat milk significantly affected the pH and acidity of fermented milk. More specifically, the use of goat milk led to fermented milk with lower pH values and higher acidities than fermented milk with cow milk. The sensory evaluation ascertained the overall quality of fermented milk with a mixture of cow and goat milk, which scored similar values to the commercial sample.


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