scholarly journals DEVELOPMENT OF OPTICAL TRANSDUCER ON FABRICS FOR THE APPLICATION OF LIQUID CLUSTERING USING REFLECTANCE SPECTROSCOPY

2021 ◽  
Vol 5 (2) ◽  
Author(s):  
Viona Hazar Briliana ◽  
Totok Mujiono
Keyword(s):  
Light Source ◽  
Monochromatic Light ◽  
Reflectance Spectroscopy ◽  
Wearable Device ◽  
Light Scatter ◽  
Optic System ◽  
Pca Method ◽  
The Difference ◽  
Scatter Intensity ◽  
Optical Transducer

Recently, usage of fabrics as wearable device, along with their applications are increasing, one example being the detection of bio-analyzes such as blood or sweat. One method used to observe the properties of the material of a fabric is to use the Refcletance Spectroscopy, in which excitation of monochromatic light with a specific wavelength is given to a fabrics. Intensity value is then processed using the PCA method in order to obtain the pattern of the difference between each substrate. The proposed transducer optic system consists of 405nm blueviolet laser as the light source, biconvex lens, Adafruit AS7262 light detector, and Arduino. This system can only detect the difference in substrate content from the occurring light scatter. This system can be applied to various kinds of fabric wearable material with differing scatter intensity values depending on the kind of fabrics. Softer kind of fabric is proposed as material for the wearable device because it gives a high scatter intensity value and constant values in every repetation which results in better data reading.Keywords: clustering, optical, reflectance, spectroscopy, transducer, wearable.

scholarly journals Contamination-resistant, rapid emulsion-based isothermal nucleic acid amplification with Mie-scatter inspired light scatter analysis for bacterial identification

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alexander S. Day ◽  
Tiffany-Heather Ulep ◽  
Elizabeth Budiman ◽  
Laurel Dieckhaus ◽  
Babak Safavinia ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Detection ◽  
Light Scatter ◽  
Emulsion Droplets ◽  
Assay Performance ◽  
Bacterial Adsorption ◽  
Scatter Intensity ◽  
The Impact ◽  
Theory Light

AbstractAn emulsion loop-mediated isothermal amplification (eLAMP) platform was developed to reduce the impact that contamination has on assay performance. Ongoing LAMP reactions within the emulsion droplets cause a decrease in interfacial tension, causing a decrease in droplet size, which results in decreased light scatter intensity due to Mie theory. Light scatter intensity was monitored via spectrophotometers and fiber optic cables placed at 30° and 60°. Light scatter intensities collected at 3 min, 30° were able to statistically differentiate 103 and 106 CFU/µL initial Escherichia coli O157:H7 concentrations compared to NTC (0 CFU/µL), while the intensity at 60° were able to statistically differentiate 106 CFU/µL initial concentrations and NTC. Control experiments were conducted to validate nucleic acid detection versus bacterial adsorption, finding that the light scatter intensities change is due specifically to ongoing LAMP amplification. After inducing contamination of bulk LAMP reagents, specificity lowered to 0% with conventional LAMP, while the eLAMP platform showed 87.5% specificity. We have demonstrated the use of angle-dependent light scatter intensity as a means of real-time monitoring of an emulsion LAMP platform and fabricated a smartphone-based monitoring system that showed similar trends as spectrophotometer light scatter data, validating the technology for a field deployable platform.

scholarly journals Deteksi Formalin Pada Buah Tomat (Lycopersicum esculentum Mill) Dengan Teknologi Hidung Elektronik (Electronic Nose)

2019 ◽  
Vol 4 (2) ◽  
pp. 359-366
Author(s):  
Irfan Maibriadi ◽  
Ratna Ratna ◽  
Agus Arip Munawar
Keyword(s):  
Principal Component Analysis ◽  
Electronic Nose ◽  
Principal Component ◽  
Component Analysis ◽  
Lycopersicum Esculentum ◽  
Olfactory Ability ◽  
Pca Method ◽  
The Difference ◽  
Electronic Nose Technology

Abstrak,  Tujuan dari penelitian ini adalah mendeteksi kandungan dan kadar formalin pada buah tomat dengan menggunakan instrument berbasis teknologi Electronic nose. Penelitian ini menggunakan buah tomat yang telah direndam dengan formalin dengan kadar 0.5%, 1%, 2%, 3%, 4%, dan buah tomat tanpa perendaman dengan formalin (0%). Jumlah sampel yang digunakan pada penelitian ini adalah sebanyak 18 sampel. Pengukuran spektrum beras menggunakan sensor Piezoelectric Tranducer. Klasifikasi data spektrum buah tomat menggunakan metode Principal Component Analysis (PCA) dengan pretreatment nya adalah Gap Reduction. Hasil penelitian ini diperoleh yaitu: Hidung elektronik mulai merespon aroma formalin pada buah tomat pada detik ke-8.14, dan dapat mengklasifikasikan kandungan dan kadar formalin pada buah tomat pada detik ke 25.77. Hidung elektronik yang dikombinasikan dengan metode principal component analysis (PCA) telah berhasil mendeteksikandungan dan kadar formalin pada buah tomat dengan tingkat keberhasilan sebesar 99% (PC-1 sebesar 93% dan PC-2 sebesar 6%). Perbedaan kadar formalin menjadi faktor utama yang menyebabkan Elektronik nose mampu membedakan sampel buah tomat yang diuji, karena semakin tinggi kadar formalin pada buah tomat maka aroma khas dari buah tomat pun semakin menghilang, sehingga Electronic nose yang berbasis kemampuan penciuman dapat membedakannya.Detect Formaldehyde on Tomato (Lycopersicum esculentum Mill) With Electronic Nose TechnologyAbstract, The purpose of this study is to detect the contents and levels of formalin in tomatoes by using instruments based on Electronic nose technology. This study used tomatoes that have been soaked in formalin with a concentration of 0.5%, 1%, 2%, 3%, 4%, 5% and tomatoes without soaking with formalin (0%). The samples in this study were 18 samples. The measurements of the intensity on tomatoes aroma were using Piezoelectric Transducer sensors. The classification of tomato spectrum data was using the Principal Component Analysis (PCA) method with Gap Reduction pretreatment. The results of this study were obtained: the Electronic nose began to respond the smell of formalin on tomatoes at 8.14 seconds, and it could classify the content and formalin levels in tomatoes at 25.77 seconds. Electronic nose combined with the principal component analysis (PCA) method have successfully detected the content and levels of formalin in tomatoes with a success rate at 99% (PC-1 of 93% and PC-2 of 6%). The difference of grade formalin levels is the main factor that causes Electronic nose to be able to distinguish the tomato samples tested, because the higher of formalin content in tomatoes, the distinctive of tomatoes aroma is increasingly disappearing. Thereby, the Electronic nose based on  the olfactory ability can distinguish them. 

Introduction to light absorption: visible and ultraviolet spectra

2000 ◽  
Author(s):  
Robert K. Poole ◽  
Uldis Kalnenieks
Keyword(s):  
Electromagnetic Radiation ◽  
Light Absorption ◽  
Energy State ◽  
Monochromatic Light ◽  
Heat Radiation ◽  
Infrared Light ◽  
Visible Radiation ◽  
Absorption Of Light ◽  
The Difference

Light is a form of electromagnetic radiation, usually a mixture of waves having different wavelengths. The wavelength of light, expressed by the symbol λ, is defined as the distance between two crests (or troughs) of a wave, measured in the direction of its progression. The unit used is the nanometre (nm, 10-9 m). Light that the human eye can sense is called visible light. Each colour that we perceive corresponds to a certain wavelength band in the 400-700 nm region. Spectrophotometry in its biochemical applications is generally concerned with the ultraviolet (UV, 185-400 nm), visible (400-700 nm) and infrared (700-15 000 nm) regions of the electromagnetic radiation spectrum, the former two being most common in laboratory practice. The wavelength of light is inversely related to its energy (E), according to the equation: . . . E = ch/ λ . . . where c denotes the speed of light, and h is Planck’s constant. UV radiation, therefore, has greater energy than the visible, and visible radiation has greater energy than the infrared. Light of certain wavelengths can be selectively absorbed by a substance according to its molecular structure. Absorption of light energy occurs when the incident photon carries energy equal to the difference in energy between two allowed states of the valency electrons, the photon promoting the transition of an electron from the lower to the higher energy state. Thus biochemical spectrophotometry may be referred to as electronic absorption spectroscopy. The excited electrons afterwards lose energy by the process of heat radiation, and return to the initial ground state. An absorption spectrum is obtained by successively changing the wavelength of monochromatic light falling on the substance, and recording the change of light absorption. Spectra are presented by plotting the wavelengths (generally nm or μm) on the abscissa and the degree of absorption (transmittance or absorbance) on the ordinate. For more information on the theory of light absorption, see Brown (1) and Chapters 2, 3 and 4. The most widespread use of UV and visible spectroscopy in biochemistry is in the quantitative determination of absorbing species (chromophores), known as spectrophotometry.

Analysis of chromosome movement in crane fly spermatocytes by ultraviolet microbeam irradiation of individual chromosomal spindle fibres. I. General results

1981 ◽  
Vol 59 (9) ◽  
pp. 770-776 ◽  
Author(s):  
Peggy J. Sillers ◽  
Arthur Forer
Keyword(s):  
Ultraviolet Light ◽  
Monochromatic Light ◽  
The Other ◽  
Spindle Fibre ◽  
Chromosome Movement ◽  
Opposite Pole ◽  
Other Hand ◽  
Ultraviolet Microbeam ◽  
The Difference ◽  
Spindle Fibres

Single chromosomal spindle fibres in anaphase Nephrotoma ferruginea (crane fly) spermatocytes were irradiated with monochromatic ultraviolet light focussed to a 4-μm spot by means of an ultraviolet microbeam apparatus. The movement of the half-bivalent associated with the irradiated spindle fibre was either unaffected or the half-bivalent stopped moving; i.e., the effect was all-or-none. When the half-bivalent associated with the irradiated spindle fibre did stop moving, the partner half-bivalent moving towards the opposite pole (i.e., the half-bivalent with which the first half-bivalent was previously paired) also stopped moving: all other half-bivalents moved normally. In over 90% of the 69 cases the movements of the two half-bivalents were only temporarily blocked; when movement resumed both half-bivalents resumed movement at the same time, after stoppage times ranging from 2 min to more than 15 min. In a few cases the half-bivalents never resumed poleward motion.When half-bivalents that had stopped movement finally resumed movement they often did not reach the poles; i.e., they "lagged" and remained separate from the other chromosomes. This result occurred only in spermatocytes of N. ferruginea. In spermatocytes of N. suturalis or N. abbreviata, on the other hand, the stopped half-bivalents did not lag but always reached the poles.Half-bivalent pairs that stopped moving in N. ferruginea spermatocytes did so for shorter times than did those previously reported (after irradiation of chromosomal spindle fibres) in N. suturalis spermatocytes. We suggest that the difference is due to our use of monochromatic ultraviolet light as opposed to the previous use of heterochromatic ultraviolet light. We assume that different wavelengths of monochromatic light produce different effects, that any given monochromatic irradiation produces only one effect (albeit different effects at different wavelengths), but that heterochromatic irradiations can produce multiple effects.Irradiation of the interzone (between separating half-bivalents) had no effect on the chromosome-to-pole movements of the half-bivalents. Therefore the stoppage of movement of half-bivalent pairs is specific for irradiation of chromosomal spindle fibres. On the other hand, irradiation of the interzone often blocked pole-to-pole elongation.

Comparison of luminous intensity distributions

2016 ◽  
Vol 49 (1) ◽  
pp. 62-83 ◽  
Author(s):  
F Gassmann ◽  
U Krueger ◽  
T Bergen ◽  
F Schmidt
Keyword(s):  
Structural Properties ◽  
Light Source ◽  
Radiation Characteristic ◽  
Luminous Flux ◽  
Luminous Intensity ◽  
Measuring System ◽  
Operating Parameters ◽  
Data Noise ◽  
Flux Data ◽  
The Difference

Luminous intensity distributions enable an evaluation of the spatial radiation characteristic of a light source. This radiation characteristic is determined by the structural properties of the light source, its operating parameters and the properties of the measuring system. This paper describes some possible methods and rules for comparing luminous intensity distributions. The focus is on the development of calculation rules for quantifying the differences between two luminous intensity distributions. The difference measures developed allow the user to establish an objective comparison between luminous intensity distributions, this comparison being completely independent of the measuring system, the properties of the luminous intensity distributions and the users themselves. Further, the dependence of the properties of luminous intensity distributions resulting from measurement practice, such as adjustment uncertainties, regions that cannot be covered or measured, deviations of the total luminous flux, data noise and resolution differences, are discussed, and appropriate pre-processing and correction steps proposed. In addition, various visualisations of the differences between two luminous intensity distributions are demonstrated and the functionality of the difference measures developed is documented.

scholarly journals Environment and Sporulation in Phytopathogenic Fungi IV. The Effect of Light on the- Formation of Conidia of Peronospora Tabacina Adam

1963 ◽  
Vol 16 (1) ◽  
pp. 88 ◽  
Author(s):  
IAM Cruickshank
Keyword(s):  
Light Source ◽  
Monochromatic Light ◽  
Continuous Light ◽  
Visible Spectrum ◽  
Phytopathogenic Fungi ◽  
Time Of Day ◽  
Light Conditions ◽  
Low Light ◽  
Maximal Inhibition ◽  
Effect Of Light

Using a leaf�disk technique, an analysis of the effect of light on the sporulation intensity of P. tabacina was carried out. The following points were demonstrated: (I) Under conditions of continuous light, sporulation of P. tabacina is sensitive to very low light intensities. ED5!1 for inhibition of sporulation was 16 ftvV cm- 2 for incandescent light (4 f,c.), and 0�58 p.W cm-2 for a monochromatic light source (469 mpo) in the region of maximum effectiveness. (2) Dark treatments induced sporulation under otherwise continuous light conditions. The response was directly proportional to the length of the exposure to darkness over the period 1�5-7 hr. (3) The time of day at which sporulation occurred could be modified by adjustment of the time of day at which darkness was initiated. (4) Within the visible spectrum, the region exerting maximal inhibition on sporulation occurred at 450-525 mJL.

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