Effect of Ozonated Water on Ochratoxin A levels in Locally Broiler Meat in Baghdad Province

The main objectives of the present research were to determination of Ochratoxin A in locally broiler meat sold in markets of Baghdad province by ELISA, effect of ozonated water treatment (0.5ppm/15 min.) on the level of Ochratoxin A in positive samples and finally determination of Ochratoxin A after ozonated water treatment. A total of 50 samples were collected randomly from various markets located in different locations of Baghdad province from each sector Al-Kirkh and Al-Rusafa during November 2017 to March 2018. All meat samples were positive for Ochratoxin A and the result showed that there were a significant differences (P ≤ 0.05) in the residual levels (ppm) of Ochratoxin A between Al-Kirkh and Al-Rusafa before and after ozonated water treatment. The highest Mean ± SE values were recorded in Al-Rusafa (0.648 ± 0.0020), followed by Al-Kirkh (0.636 ± 0.0025) before ozonated water treatment, while the highest Mean ± SE values after ozonated water treatment were recorded in Al-Rusafa (0.346 ± 0.0025), followed by Al-Kirkh (0.332 ± 0.0049). The lowest Mean ± SE values of Ochratoxin A before ozonated water treatment were recorded in Al-Rusafa (0.502 ± 0.0040), followed by Al-Kirkh (0.504 ± 0.0058), while the lowest Mean ± SE values of Ochratoxin A after ozonated water treatment were recorded in Al-Rusafa, followed by Al-Kirkh also at (0.264 ± 0.0040), and (0.266 ± 0.0025) respectively. This research indicated that the poultry meat treated with ozonated water has the advantages methods that it did not affect the colour and texture characteristics of the meat, can be used to eliminate or reduce Ochratoxin A residues at the same time, and can be used in any slaughter house without the need to modify the design of the buildings.

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RESEARCH OF SENSORY CHARACTERISTICS OF POULTRY MEAT OBTAINED USING VARIOUS METHODS OF STUN

Известия вузов. Пищевая технология ◽

10.26297/0579-3009.2020.4.21 ◽

2020 ◽

Author(s):

Д.Б. ШАЛГИНБАЕВ ◽

Р.У. УАЖАНОВА ◽

Л.В. АНТИПОВА

Keyword(s):

Optical Resolution ◽

Sensory Characteristics ◽

Poultry Meat ◽

Test Material ◽

Quantitative Composition ◽

Meat Sample ◽

Broiler Meat ◽

Aroma Characteristics ◽

Manometric Method ◽

Meat Samples

Исследованы цветовые и ароматические характеристики мяса бройлеров, полученного оглушением птицы углекислым газом и электрическим способом. Объектом исследования были образцы мяса бройлеров, произведенного в Казахстане электрооглушением – образец 1; и в РФ: газовым оглушением – образец 2; электрооглушением – образец 3. Каждый из образцов был представлен мясом из 2 частей тушки: бедра и грудки. Образцы мяса сканировали в цветовом режиме TrueCоlоr, оптическое разрешение 300 dpi, размер не менее 500 ґ 500 pix. Цифровое изображение обрабатывали с помощью программы ImаgeJ 1.46. Численные характеристики окраски исследованных образцов мяса птицы определяли сканерометрическим методом с использованием планшетного сканера. Изображения обрабатывали на компьютере в режиме RGB. Исследование запаха мяса проводили на анализаторе запахов МАГ-8 по методике «электронный нос» в режиме фронтальной подачи пробы и инжекторного ввода равновесной газовой фазы над исследуемым материалом. В качестве измерительного массива применяли набор из 8 сенсоров с наибольшей чувствительностью к различным классам легколетучих органических соединений. Установлено, что цветометрические характеристики образцов мяса, полученного разными способами оглушения птицы, идентичны. Различия в качественном и количественном составе аромата образца мяса 2, полученного оглушением птицы в газовой среде, и образца мяса 3, полученного электрооглушением птицы, составляют от 2 до 10%, что не позволяет говорить о существенном изменении в составе легколетучих соединений, выделяющихся из мяса. Сенсорные характеристики исследованных образцов мяса, полученного разными способами оглушения птицы, практически идентичны. Это доказывает возможность применения более эффективной технологии газового оглушения. The color and aroma characteristics of broiler meat obtained by stunning poultry with carbon dioxide and electric method were studied. Samples of broiler meat produced in Kazakhstan by electric deafening – sample 1; in the Russian Federation: gas deafening – sample 2; electric deafening – sample 3 were the object of research. Each of the samples was represented by meat from 2 parts of the carcass: thighs and breasts. Meat samples were scanned in TrueColor color mode, with an optical resolution of 300 dpi and a size of at least 500 ґ 500 pix. The digital image was processed using ImageJ 1.46. Numerical characteristics of the color of the studied samples of poultry meat were determined by the manometric method using a flatbed scanner. Images were processed on a computer in RGB mode. The study of the smell of meat was carried out on the MAG-8 smell analyzer using the "electronic nose" method in the mode of frontal sample delivery and injection of the equilibrium gas phase over the test material. A set of 8 sensors. the most sensitive to various classes of volatile organic compounds were used as a measuring array. It was found that the colorometric characteristics of meat samples obtained by different methods of stun poultry are identical. Differences in the qualitative and quantitative composition of the flavor of meat sample 2, obtained by stun poultry in a gas environment, and meat sample 3, obtained by electric stun poultry, are from 2 to 10%, which does not allow us to talk about a significant change in the composition of low-volatile compounds released from the meat, The sensory characteristics of the studied meat samples obtained by different methods of stun poultry are almost identical. This proves the possibility of using more effective gas stun technology.

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Prevalence and antibiotic susceptibility pattern of Campylobacter species isolated from broiler chicken meat samples in district Bannu, Pakistan

Journal of Food Safety and Hygiene ◽

10.18502/jfsh.v5i4.5704 ◽

2021 ◽

Author(s):

Inam Ullah ◽

Hajra Khurshid ◽

Nadeem Ullah ◽

Israr Aziz ◽

Muhammad Junaid Khan ◽

...

Keyword(s):

Public Health ◽

Developed Countries ◽

Cloacal Swab ◽

Poultry Meat ◽

Diffusion Method ◽

Broiler Meat ◽

Cross Sectional ◽

Khyber Pakhtunkhwa ◽

Campylobacter Species ◽

Meat Samples

The campylobacter genus of bacteria is important in public health as it comprises many species causing diarrhea in humans. Poultry and their products are recognized as vital causes of campylobacteriosis in humans. For bacterial food-borne diseases, Campylobacter is considered as the leading cause. Higher prevalence has been reported in developed countries. Our study was a cross-sectional study directed to determine the prevalence of Campylobacter species in retail broiler meat in the Bannu district of Khyber Pakhtunkhwa, Pakistan, from January to June 2018. A total of 200 poultry meat samples were collected from four different areas of district Bannu Khyber Pakhtunkhwa province of Pakistan that includes Lakki gate, Tanchi bazar, Bannu Township and Mangal milla. Mueller-Hinton medium was used for disc diffusion method to determine antibiotic resistance of Campylobacter species. Amongst 200 broiler meat samples, 60 (30%) samples were found positive for Campylobacter species. The highest prevalence was observed in samples from Bannu Township (50%) while lowest prevalence (12%) was observed in samples from Mangal milla broiler meat samples. Amongst different types of meat samples, highest prevalence was found in thigh meat (46%), while lowest prevalence was observed in cloacal swab (20%). Highest resistance was observed against Amoxicillin (AMX) 80% while the resistance observed against other antibiotics were Ampicillin (AMP) 70%, Tetracycline (TET) 65%, Sulphamethoxazole + Trimethoprim (SXT) 60%, Chloramphenicol (CHL) 56.66%, Clarithromycin (CLR) 50%, Streptomycin (STR) 40%, Gentamycin (GEN) 36.66%, Ofloxacin (OFX) 20%, Ciprofloxacin (CIP) 15%, Levofloxacin (LEV) 15% and Azithromycin (AZM) 10%. The lowest resistance was observed against Ceftriaxone (CRO) 5%. Our Study concludes that Campylobacter species is prevalent highly in district Bannu, and it might be a hazard to public health.

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The influence of electrogalvanic device on scaling

Open Chemistry ◽

10.2478/s11532-013-0206-7 ◽

2013 ◽

Vol 11 (5) ◽

pp. 698-705 ◽

Cited By ~ 1

Author(s):

Marjana Simonič ◽

Irena Ban

Keyword(s):

Drinking Water ◽

Water Treatment ◽

Water Samples ◽

Treatment Method ◽

Aragonite Crystal ◽

Physico Chemical ◽

Before And After ◽

Drinking Water Samples

AbstractThe use of an electrogalvanic device for scale neutralisation is descibed in this paper. Physico-chemical analyses were performed before and after the treatment. The results were compared with those obtained by using magnetic water treatment device. By measuring some individual parameters and the implementation of chemical analysis, the satisfactory functioning of the electrogalvanic device was demonstrated. The quality of drinking water did not change much after the water treatment method. The results of determination of calcium carbonate saturating index showed that the raw drinking water is in carbonate equlibrium as well as both treated water samples. The calcite/aragonite ratio was studied by means of microscopy and X-ray powder diffraction. Inspection of crystals formed during the experiments with microscopy indicated that aragonite crystal structure of the precipitates prevailed over the calcite stucture. The diffractograms showed that the share of aragonite increased after using the electrogalvanic device compared with raw drinking water samples where the share of calcite was higher.

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Effect of Fermented Cereals, Probiotics, and Phytase on the Sensory Quality of Poultry Meat

Scientia Agriculturae Bohemica ◽

10.2478/sab-2018-0029 ◽

2018 ◽

Vol 49 (3) ◽

pp. 225-235 ◽

Cited By ~ 1

Author(s):

S. Yasar ◽

E. Boselli ◽

F. Rossetti ◽

M.S. Gok

Keyword(s):

Broiler Chickens ◽

Sensory Quality ◽

Control Diet ◽

Coturnix Japonica ◽

Poultry Meat ◽

Broiler Meat ◽

Japanese Quails ◽

Fermented Grains ◽

Meat Samples

Abstract The sensory properties of poultry meat obtained from meat-type broiler chickens fed with fermented cereals and two different fermented supplements, and Japanese quails (Coturnix japonica) fed diets including fermented cereals (wheat, barley or oats), three probiotics (from E. faecium, B. subtilis, S. cerevisiae) and two different phytase preparations were evaluated. Fermented grains (particularly wheat and barley) decreased the sensory quality of meat. The use of three probiotic products and phytase did not negatively influence the sensory quality of the quail meats. The diets containing S. cerevisiae (probiotic) produced a highly appreciated meat. The less preferred meat samples were obtained from the quails fed diets containing fermented wheat, fermented barley, and E. faecium, due to the off-flavour and odour as well as tanginess. The supplementation of naturally or yeast-fermented liquid whey and lemon pomace was very effective to overcome the bad sensory quality (fish and metallic off-flavour and taste) of the broiler meat obtained by administering the control diet (rich in vegetable oil and fermented wheat).

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Prevalence and Characterization of ESBL/AmpC Producing Escherichia coli from Fresh Meat in Portugal

Antibiotics ◽

10.3390/antibiotics10111333 ◽

2021 ◽

Vol 10 (11) ◽

pp. 1333

Author(s):

Lurdes Clemente ◽

Célia Leão ◽

Laura Moura ◽

Teresa Albuquerque ◽

Ana Amaro

Keyword(s):

Escherichia Coli ◽

Acquired Resistance ◽

Multidrug Resistant ◽

Resistance Mechanisms ◽

Selective Medium ◽

Poultry Meat ◽

Broiler Meat ◽

E Coli ◽

Antimicrobial Resistance Genes ◽

Meat Samples

The present study aimed to characterize the extended-spectrum β-lactamases and plasmid-mediated AmpC β-lactamases (ESBL/PMAβ) among Escherichia coli producers isolated from beef, pork, and poultry meat collected at retail, in Portugal. A total of 638 meat samples were collected and inoculated on selective medium for the search of E. coli resistant to 3rd generation cephalosporins. Isolates were characterized by antimicrobial susceptibility testing, molecular assays targeting ESBL/AmpC, plasmid-mediated quinolone resistance (PMQR), and plasmid-mediated colistin resistance (PMCR) encoding genes. The highest frequency of E. coli non-wild type to 3rd generation cephalosporins and fluoroquinolones was observed in broiler meat (30.3% and 93.3%, respectively). Overall, a diversity of acquired resistance mechanisms, were detected: blaESBL [blaCTX-M-1 (n = 19), blaCTX-M-15 (n = 4), blaCTX-M-32 (n = 12), blaCTX-M-55 (n = 8), blaCTX-M-65 (n = 4), blaCTX-M-27 (n = 2), blaCTX-M-9 (n = 1), blaCTX-M-14 (n = 11), blaSHV-12 (n = 27), blaTEM-52 (n = 1)], blaPMAβ [blaCMY-2 (n = 8)], PMQR [qnrB (n = 27), qnrS (n = 21) and aac(6’)-Ib-type (n = 4)] and PMCR [mcr-1 (n = 8)]. Our study highlights that consumers may be exposed through the food chain to multidrug-resistant E. coli carrying diverse plasmid-mediated antimicrobial resistance genes, posing a great hazard to food safety and a public health risk.

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The Plasmin Inhibitors of Plasma

Thrombosis and Haemostasis ◽

10.1055/s-0038-1655579 ◽

1964 ◽

Vol 12 (01) ◽

pp. 119-125 ◽

Cited By ~ 9

Author(s):

Y Shamash ◽

A Rimon

Keyword(s):

Human Plasma ◽

New Method ◽

Normal Amount ◽

Caseinolytic Activity ◽

Before And After ◽

Plasmin Inhibitors

SummaryA new method for the assay of plasmin inhibitors in human plasma is described. The method consists of determination of the caseinolytic activity of a standard plasmin solution before and after incubation with the inhibitor, with lysine added to the mixture as a stabilizer of plasmin. Using this method, it was found that plasma contains enough inhibitors to inactivate 30 caseinolytic units of plasmin, or 10 times the normal amount of plasminogen in human plasma.

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FRACTIONAL DETERMINATIONS OF URINARY 17-KETOSTEROIDS IN HYPEREMESIS GRAVIDARUM

Acta Endocrinologica ◽

10.1530/acta.0.0410123 ◽

1962 ◽

Vol 41 (1) ◽

pp. 123-128 ◽

Cited By ~ 6

Author(s):

Pentti A. Järvinen ◽

Sykkö Pesonen ◽

Pirkko Väänänen

Keyword(s):

Hyperemesis Gravidarum ◽

First Trimester ◽

Control Group ◽

Before And After ◽

Daily Urine ◽

First Trimester Of Pregnancy ◽

The Difference

ABSTRACT The fractional determination of 17-ketosteroids in the daily urine was performed in nine cases of hyperemesis gravidarum and in four control cases, in the first trimester of pregnancy both before and after corticotrophin administration. The excretion of total 17-KS is similar in the two groups. Only in the hyperemesis group does the excretion of total 17-KS increase significantly after corticotrophin administration. The fractional determination reveals no difference between the two groups of patients with regard to the values of the fractions U (unidentified 17-KS), A (androsterone) and Rest (11-oxygenated 17-KS). The excretion of dehydroepiandrosterone is significantly higher in the hyperemesis group than in the control group. The excretion of androstanolone seems to be lower in the hyperemesis group than in the control group, but the difference is not statistically significant. The differences in the correlation between dehydroepiandrosterone and androstanolone in the two groups is significant. The high excretion of dehydroepiandrosterone and low excretion of androstanolone in cases of hyperemesis gravidarum is a sign of adrenal dysfunction.

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DETERMINATION OF BLOOD CORTICOIDS USING THE IN VITRO RESIN UPTAKE OF 3H-PREDNISOLONE

Acta Endocrinologica ◽

10.1530/acta.0.0570023 ◽

1968 ◽

Vol 57 (1) ◽

pp. 23-32 ◽

Cited By ~ 2

Author(s):

Hironori Nakajima ◽

Mitsunori Murala ◽

Masumitsu Nakata ◽

Takeshi Naruse ◽

Seiji Kubo

Keyword(s):

Thyroid Function Test ◽

Normal Subjects ◽

Adrenal Function ◽

Function Test ◽

Before And After ◽

Adrenal Response ◽

Suppression Test

ABSTRACT The in vitro resin uptake of 3H-prednisolone was used for the determination of blood cortisol after addition of radioactive prednisolone followed by Amberlite CG 400 Type 1 to the test serum, and incubation of the mixture. The radioactivity of the supernatant was compared before and after the addition of the resin. The principle of this method is similar to that of the 131I-triiodothyronine resin uptake for the thyroid function test. The tests for the specificity, reproducibility and sensitivity gave satisfactory results. The mean basal value ± SD of the 3H-prednisolone resin uptake was 35.3 ± 9.2% in normal subjects, and 27.1 ± 4.8% in pregnant women. This method was valid in various adrenal function tests, i. e. the adrenal circadian rhythm, corticotrophin (ACTH) test, dexamethasone suppression test and the adrenal response to lysine-8-vasopressin. It proved to be a sensitive indicator of the adrenal function. These results suggest that this method should be useful for a routine adrenal function test.

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