Bioremediation of Petroleum hydrocarbon by using Pseudomonas species isolated from Petroleum contaminated soil

According to the previous experience on remediation experiment in laboratory and field, the study on microgial ecological remediation of petroleum contaminated soil in Puyang oilfield was carried out under 3 different conditions. Here the results from remediation show that the technology of microbe cooperated with alfalfa, single microbe technology and single alfalfa technology all possess remedial effect on petroleum contaminated soil through 99-day period remediation, and the technology of microbe cooperated with alfalfa creates the best remedial mission with petroleum hydrocarbon degradation rate of 65.27%, while the rest single technologies exerts degradation rate of about 40% on petroleum hydrocarbon, Moreover, the experiment results indicate that few nutriment such as soluble salt, NO3-, Cl-,etc can infiltrate into the lower soil layer(50cm). However, the distinct increase of NH4+ in the second and third remediation area may attribute to abundance fertilizer transportation into depth soil layer due to the nitrogen fixation of alfalfa’s roots. In addition, we also find that the remediation effect can be impacted by the factors containing tempertation, water, oxygen, nutriment and mini-geo-enviroment.

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Effects of surfactants on the remediation of petroleum contaminated soil and surface hydrophobicity of petroleum hydrocarbon degrading flora

Environmental Engineering Research ◽

10.4491/eer.2020.384 ◽

2020 ◽

Vol 26 (5) ◽

pp. 200384-0

Author(s):

Jianbo Liu ◽

Liming Xu ◽

Feifei Zhu ◽

Shouhao Jia

Keyword(s):

Contaminated Soil ◽

Petroleum Hydrocarbons ◽

Petroleum Hydrocarbon ◽

Initial Conditions ◽

Removal Rate ◽

Surface Hydrophobicity ◽

Orthogonal Experiments ◽

Petroleum Contaminated Soil ◽

Application Potential ◽

High Removal Rate

It has been proven that surfactants used in the remediation of petroleum hydrocarbon contaminated soil have great application potential. In this study, the effects of five surfactants (SDBS, Tween80, Tween60, rhamnolipid and TRS-1) on leaching of petroleum hydrocarbons from soil were investigated through orthogonal experiments, and petroleum hydrocarbon components were analyzed by GC/MS. The effects of surfactants on the degradation of petroleum hydrocarbon were analyzed by the changes of microbial growth curve and surface hydrophobicity. The results showed that surfactant type, temperature and surfactant concentration had significant effects on the removal rate of petroleum hydrocarbon. Tween80, rhamnolipid and TRS-1 have good bio-friendliness and a high removal rate of petroleum hydrocarbons (up to 65%), suitable for the restoration of the soil used in the experiment And Surfactants exhibited a higher removal rate for small molecules and petroleum hydrocarbons with odd carbon atoms. Surfactants have a certain modification effect on the surface of relatively hydrophilic bacteria under the initial conditions, making their surface properties develop in the direction of enhanced hydrophobicity, and the hydrophobicity has increased from less than 20% to about 40%.

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Bioaugmentation and Biostimulation of Total Petroleum Hydrocarbon Degradation in a Petroleum-contaminated Soil with Fungi Isolated from Olive Oil Effluent

Water Air & Soil Pollution ◽

10.1007/s11270-019-4127-8 ◽

2019 ◽

Vol 230 (3) ◽

Cited By ~ 7

Author(s):

Abduelbaset M. A. Essabri ◽

Nur Pasaoglulari Aydinlik ◽

Ndifreke Etuk Williams

Keyword(s):

Olive Oil ◽

Contaminated Soil ◽

Petroleum Hydrocarbon ◽

Total Petroleum Hydrocarbon ◽

Hydrocarbon Degradation ◽

Petroleum Contaminated Soil

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Characterization on the rhizoremediation of petroleum contaminated soil as affected by different influencing factors

Biogeosciences Discussions ◽

10.5194/bgd-7-4665-2010 ◽

2010 ◽

Vol 7 (3) ◽

pp. 4665-4688 ◽

Cited By ~ 14

Author(s):

J. Tang ◽

R. Wang ◽

X. Niu ◽

M. Wang ◽

Q. Zhou

Keyword(s):

Plant Growth ◽

Influencing Factors ◽

Tall Fescue ◽

Contaminated Soil ◽

Petroleum Hydrocarbon ◽

Initial Period ◽

Plant Growth Promoting Rhizobacteria ◽

Slow Increase ◽

Continuous Increase ◽

Petroleum Contaminated Soil

Abstract. In this paper, pilot experiments were conducted to analyze the effect of different environmental factors on the rhizoremediation of petroleum contaminated soil. Different plant species (cotton, ryegrass, tall fescue, and alfalfa), addition of fertilizer, different concentration of TPH in soil, bioaugmentation with effective microbial agent (EMA) and PGPR, and remediation time were tested as influencing factors during bioremediation process of Total Petroleum Hydrocarbon (TPH). The result shows that the remediation process can be enhanced by different plants species with the following order: tall fescue > ryegrass > alfalfa > cotton. The degradation rate of TPH increased with increased fertilizer addition and moderate level of 20 g/m2 urea is best for both plant growth and TPH remediation. High TPH content is toxic to plant growth and inhibits the degradation of petroleum hydrocarbon with 5% TPH content showing the best degradation result in soil planted with ryegrass. Bioaugmentation with different bacteria and plant growth promoting rhizobacteria (PGPR) showed the following results for TPH degradation: cotton + EMA + PGPR > cotton + EMA > cotton + PGPR > cotton > control. Rapid degradation of TPH was found at the initial period of remediation caused by the activity of microorganisms, continuous increase was found from 30–90 d period and slow increase was found from 90 to 150 d. The result suggests that rhizoremediation can be enhanced with the proper control of different influencing factors that affect both plant growth and microbial activity in the rhizosphere environment.

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Hydrocarbon treatability study of Antarctica soil with Fenton’s reagent

10.21079/11681/41260 ◽

2021 ◽

Author(s):

Samuel Beal ◽

Ashley Mossell ◽

Jay Clausen

Keyword(s):

Environmental Health ◽

Contaminated Soil ◽

Contaminated Soils ◽

Petroleum Hydrocarbon ◽

Total Petroleum Hydrocarbon ◽

Fenton’S Reagent ◽

Fenton's Reagent ◽

Mcmurdo Station ◽

Petroleum Contaminated Soil ◽

Annual International Conference

The study objectives were to determine the effectiveness of Fenton’s Reagent and Modified Fenton’s Reagent in reducing Total Petroleum Hydrocarbon (TPH) concentrations in petroleum-contaminated soil from McMurdo Station, Antarctica. Comparisons of the contaminated soils were made, and a treatability study was completed and documented. This material was presented at the Association for Environmental Health and Sciences Foundation (AEHS) 30th Annual International Conference on Soil, Water, Energy, and Air (Virtual) on March 25, 2021.

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Distribution of Bacterial Communities in Petroleum-Contaminated Soils from the Dagang Oilfield, China

Transactions of Tianjin University ◽

10.1007/s12209-019-00226-7 ◽

2019 ◽

Vol 26 (1) ◽

pp. 22-32 ◽

Cited By ~ 2

Author(s):

Xueke Feng ◽

Zhen Liu ◽

Xiaoqiang Jia ◽

Wenyu Lu

Keyword(s):

Bacterial Community ◽

Contaminated Soil ◽

Contaminated Soils ◽

Bacterial Communities ◽

Petroleum Hydrocarbon ◽

High Throughput Sequencing ◽

Environmental Parameters ◽

Significant Difference ◽

Petroleum Contaminated Soil ◽

Different Depths

AbstractDiversity in bacterial communities was investigated along a petroleum hydrocarbon content gradient (0–0.4043 g/g) in surface (5–10 cm) and subsurface (35–40 cm) petroleum-contaminated soil samples from the Dagang Oilfield, China. Using 16S rRNA Illumina high-throughput sequencing technology and several statistical methods, the bacterial diversity of the soil was studied. Subsequently, the environmental parameters were measured to analyze its relationship with the community variation. Nonmetric multidimensional scaling and analysis of similarities indicated a significant difference in the structure of the bacterial community between the nonpetroleum-contaminated surface and subsurface soils, but no differences were observed in different depths of petroleum-contaminated soil. Meanwhile, many significant correlations were obtained between diversity in soil bacterial community and physicochemical properties. Total petroleum hydrocarbon, total organic carbon, and total nitrogen were the three important factors that had the greatest impacts on the bacterial community distribution in the long-term petroleum-contaminated soils. Our research has provided references for the bacterial community distribution along a petroleum gradient in both surface and subsurface petroleum-contaminated soils of oilfield areas.

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Impact of Super Absorbent Polymer and Plants on Microbial Community and Petroleum Hydrocarbon Degradation in Contaminated Soil

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.807-809.353 ◽

2013 ◽

Vol 807-809 ◽

pp. 353-360 ◽

Cited By ~ 1

Author(s):

Hai Hua Jiao ◽

Zhi Hui Bai ◽

Ying Liu ◽

Kai Wang ◽

Zhan Bin Huang

Keyword(s):

Microbial Community ◽

Contaminated Soil ◽

Soil Microbial Community ◽

Petroleum Hydrocarbon ◽

Principal Component ◽

Crop Species ◽

Super Absorbent Polymer ◽

Soil Microbial ◽

Petroleum Contaminated Soil ◽

Absorbent Polymer

A greenhouse pot test, in which wheat, cabbage, spinach were cultivated separately in petroleum contaminated soil with and without super absorbent polymer (SAP), was conducted to evaluated the effect of plants and SAP on soil microbiological properties. phospholipid fatty acids (PLFAs) profiles were analyzed to reveal the microbial communities. As a measure of the functional activity of soil microbial community, the ratio of degraded to total petroleum hydrocarbon (TPH) in soil was estimated. The results indicated that SAP had an important effect on the soil microbial community and its degrading TPH activities. First, the principal component analysis (PCA) of the PLFA signatures revealed marked changes between soil with SAP and without SAP. In addition, the total amount and the profile of PLFA were significantly different between the untreated and SAP-amended soils. Using PLFA patterns as a biomarker, it was found that gram-positive bacteria (G+) were more sensitive to SAP than gram-negative bacteria (G-), and the biomass of G+ was higher in soil with SAP than in that without SAP. Second, the crop could stimulate the growth of soil microorganisms; however, the differences depended clearly on the crop species. The G+ and G- biomass was increased in cabbage, spinach soil containing SAP, but was decreased in wheat soils. The population of fungi was increased in cabbage and spinach soils containing SAP, but was decreased in wheat soil with SAP. The population of actinomycetes was decreased in all soils with SAP. Third, the ratio of degraded to TPH was slightly increased in soil with SAP treatment, but a significant change depended on the crop species. In total, 12 different PLFAs were identified, including saturated, monounsaturated, branched, and polyunsaturated species. There was a clear difference in the PLFAs composition between soils with and without SAP.

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Bacteria Associated with Petroleum Hydrocarbon Contaminated Soil from NNPC Depot

Journal of Advances in Microbiology ◽

10.9734/jamb/2020/v20i630257 ◽

2020 ◽

pp. 77-82

Author(s):

S. O. Bankole ◽

M. B. Oyedeji ◽

F. O. Akanni ◽

E. P. Chukwudebe ◽

A. O. Olatunji ◽

...

Keyword(s):

Escherichia Coli ◽

Local Government ◽

Contaminated Soil ◽

Petroleum Hydrocarbon ◽

Research Work ◽

Soil Samples ◽

Pseudomonas Sp ◽

Bacillus Sp ◽

Plate Method ◽

Pseudomonas Species

The microorganisms associated with soil polluted with petroleum hydrocarbon were isolated at NNPC depot Ibadan, in Ido Local Government Areas of Oyo State. Soil samples were taken from three different points along the point of discharge. The spread plate method was used to isolate the microorganisms found in these rivers and were later identified. From the research, seven different species of microorganism were isolated, which are Pseudomonas sp, Bacillus sp., Micrococcus sp. Staphylococcus aureaus sp., Enterobacter sp., Klebsiella sp. and Escherichia coli. Pseudomonas species were found to be more prominent in the samples collected in a research work. This suggested that the isolates are resistant to the petroleum hydrocarbon and can be recommended as candidates for the clean-up of petroleum hydrocarbon contaminated soil.

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