scholarly journals Bacteria Associated with Petroleum Hydrocarbon Contaminated Soil from NNPC Depot

2020 ◽  
pp. 77-82
Author(s):  
S. O. Bankole ◽  
M. B. Oyedeji ◽  
F. O. Akanni ◽  
E. P. Chukwudebe ◽  
A. O. Olatunji ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Local Government ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Research Work ◽  
Soil Samples ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Plate Method ◽  
Pseudomonas Species

The microorganisms associated with soil polluted with petroleum hydrocarbon were isolated at NNPC depot Ibadan, in Ido Local Government Areas of Oyo State. Soil samples were taken from three different points along the point of discharge. The spread plate method was used to isolate the microorganisms found in these rivers and were later identified. From the research, seven different species of microorganism were isolated, which are Pseudomonas sp, Bacillus sp., Micrococcus sp. Staphylococcus aureaus sp., Enterobacter sp., Klebsiella sp. and Escherichia coli. Pseudomonas species were found to be more prominent in the samples collected in a research work. This suggested that the isolates are resistant to the petroleum hydrocarbon and can be recommended as candidates for the clean-up of petroleum hydrocarbon contaminated soil.

scholarly journals Studies on decolorization of textile dye by using Pseudomonas and bacillus sp from the contaminated effluent soil samples of Kovilpatti, Thoothukudi district of Tamil Nadu

2019 ◽  
Vol 11 (1) ◽  
pp. 134-137 ◽  
Author(s):  
Kannan D ◽  
Renuga Devi ◽  
A. G. Murugesan ◽  
S. Rajan
Keyword(s):  
Contaminated Soil ◽  
Tamil Nadu ◽  
Soil Samples ◽  
Toxic Chemicals ◽  
Textile Dye ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Textile Industries ◽  
Source Of Pollution

Textile industries releasing large amount of effluent which contains textile dyes and toxic chemicals and it is one of the major source of pollution also contaminating water bodies. To remove that, bacteria have been of great attention because of their ability to treat effluent. The present study was undertaken to exploit the ability of Pseudomonassp and Bacillus sp from dye contaminated soil samples for bioremediation for dye effluent. Among the bacterial strains used in the study. Pseudomonas sp emerged out to be most potent decolorizer in comparison to Bacillus sp with the degree of decolorization of 90.0 %. Thus, it was concluded that the Pseudomonas sp had highest color removing capacity from contaminated effluent soil samples. 

scholarly journals Isolasi dan Aktivitas Antibakteri Aktinomisetes Asal Tanah Gambut Riau

2018 ◽  
Vol 10 (1) ◽  
pp. 18
Author(s):  
Tetty Marta Linda ◽  
Rodesia Mustika Roza ◽  
Rola Yuliati ◽  
Wahyuliyanti Wahyuliyanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Peat Soil ◽  
Soil Samples ◽  
Pseudomonas Sp ◽  
Plate Method ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
And Staphylococcus Aureus

The aims of this study are to isolate actinomycetes from peat soil samples, to determine the ability of actinomycetes to inhibit the growth of Gram positive bacteria (Bacillus subtilis and Staphylococcus aureus) and Gram negativebacteria (Escherichia coli and Pseudomonas sp.). A total of 14 actinomycetes strains were recovered from peat soil samples using pour plate method with Starch Casein Agar. The results showed that 11 isolates were active against B. subtilis, 8 isolates against S. aureus, 8 isolates were active against E. coli and 8 isolates againstPseudomonas sp. Two isolates (SM 1.3 and SM 1.6) were active against all bacterial targets.

scholarly journals Screening the Milieu of an abattoir for Bacteria of Public health importance in Makurdi, Benue State, Nigeria

2017 ◽  
Vol 7 (2) ◽  
pp. 1
Author(s):  
Nseabasi Maina N ◽  
E. G Vinkings ◽  
I U Bassey ◽  
A. A Unimke ◽  
L.O Abulawor
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Antibiotic Sensitivity ◽  
Soil Samples ◽  
Bacillus Species ◽  
Bacterial Cells ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Public Health Importance

The study investigated the bacteriological content in a major abattoir located approximately 300 meters from a densely populated and cultivated area along the Benue River. Enumeration of bacterial cells from samples in the study yielded relatively high mean count of 9.4 x 105 and 7.3 x 105 from effluent and soil samples respectively. Bacteria isolated from both samples included; Escherichia coli, Streptococcus sp, Salmonella sp, Pseudomonas sp, Shigellasp, Enterobacter sp, Staphylococcus sp, Bacillus sp, Brucella sp, Proteus sp, Micrococcus sp etc. Escherichia coli recorded an occurrence of 18.53% in effluents and 16.16% in soil while Proteus species and Brucella sp had an occurrence of 9.59% and 1.39% respectively in soil samples. Antibiotic sensitivity screening using seveenteen (17) antibiotics disc (Optun Nig.) viz: Tarivid (10 µg), peflacine (10 µg), Agumentin (30 µg), Gentamycin (10 µg), Streptomycin (30 µg), Ceporex (10 µg), Nalidixic acid (30 µg), Ciprofloxacin (10 µg), Norfloxacin (10 µg), Rifampicin (µg), Erythromycin (µg), Chloramphenicol (µg), Ampiclox (30 µg), Levofloxacin (10µg). A marked level of resistance was observed among the isolates. However, Escherichia coli indicated sensitivity to peflacine, Shigellasp indicated sensitivity to augmentin, Enterococcus and Bacillus species indicated sensitivity to ciprofloxacin and streptomycin respectively.

scholarly journals Pola Kuman Penyebab Ventilator Associated Pneumonia(VAP) dan Sensitivitas Terhadap Antibiotik di RSAB Harapan Kita

Sari Pediatri ◽  
2016 ◽  
Vol 13 (6) ◽  
pp. 384
Author(s):  
Retno Widyaningsih ◽  
Latre Buntaran
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Stenotrophomonas Maltophilia ◽  
Clinical Laboratory ◽  
Enterobacter Aerogenes ◽  
National Committee ◽  
Klebsiella Pneumonia ◽  
Ventilator Associated Pneumonia ◽  
Pseudomonas Sp ◽  
Bacillus Sp

Latar belakang. Peningkatan resistensi antibiotik di antara bakteri penyebab pneumonia nosokomial yang didapat di rumah sakit telah banyak dilaporkan. Jika kita tidak mengenal pola kepekaan kuman di suatu rumah sakit akan menyulitkan pemberian terapi empiris awal. Tujuan. Mengetahui profil kuman penyebab pneumonia yang didapat di rumah sakit pada anak serta uji sensitivitas terhadap beberapa antibiotik.Metode. Studi deskriptif retrospektif dengan sumber data yang berasal dari rekam medis Laboratorium Mikrobiologi RSAB Harapan Kita periode Januari hingga Juni 2010. Spesimen adalah semua spesimen saluran respiratorik dari pasien dengan diagnosis pneumonia yang dirawat. Biakan dan uji resistensi dilakukan menurut standar National Committee for Clinical Laboratory Standards(NCCLS).Hasil. Didapatkan 116 spesimen biakan dan di antaranya 4 (3,4%) steril. Dari 112 biakan positif, 79.5% di antaranya adalah bakteri gram negatif berturut-turut dari yang paling dominan adalah Pseudomonas sp.(22,4%), Pseudomonas aeruginosa(18,1%), Stenotrophomonas maltophilia(9.5%), Serratia marcescens(8,6%),Enterobacter aerogenes(7,8%), Klebsiella pneumonia, Bacillus sp., dan Escherichia coli(masing-masing 5,2%). Golongan Pseudomonasmemiliki sensitivitas terhadap ceftazidime, amikacin serta netilmicin.Kesimpulan. Basil gram negatif aerob (79,5%) merupakan mikroorganisme penyebab yang paling dominan. Ceftazidime, diikuti terhadap amikacin serta netilmicin masih mempunyai sensitivitas yang tinggi sehingga dapat dipakai sebagai terapi awal VAP.

scholarly journals Penghilangan Senyawa Fenol Oleh Bakteri Yang Diisolasi dari Area Pertambangan Minyak Bumi

2016 ◽  
Vol 17 (2) ◽  
pp. 126 ◽  
Author(s):  
Joko Prayitno
Keyword(s):  
Removal Efficiency ◽  
Contaminated Soil ◽  
Phenol Removal ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Bacterial Strains

The aim of this research was to study the effectiveness of local bacterial strains from oil-contaminated soil to degrade phenol. The study consisted of two experiments, using six individual strains and using mix of strains. Bacterial strains used in the first experiment were 1.3, 3.3 dan 8.2.1 (Bacillus sp.), strain 3.2 (Propionibacterium), strain 3.4 (Pseudomonas sp.), and strain 8.1.2 (Enterobacter sp.).Bacterial strains used in the second experiment were mix of all six strains (K6) and mix of three strains (K3) consisted of  strain 3.4, 8.1.2 and 8.2.1 with the same ratio. The experiments were conducted in 100 mL Bushnell and Haas medium containing 300-400 ppm phenol for three days.Three strains (strain 3.4, 8.1.2, dan 8.2.1) had the highest phenol removal efficiency at day 3, i.e. 99-100%. COD values were decreased to 345-393 mg/L or 56-61.3% by those three strains. Mix culture K6 effectively removed phenol form the medium, but COD value decreased to only 56.7%. The fate of COD decrease was not the same as phenol removal by these strains (either in idividual or mix cultures), because phenol was degraded into intermediate compounds. 

scholarly journals INDUCED DEGRADATION OF CRUDE OIL POLLUTED SOIL BY MICROBIAL AUGMENTATION

2017 ◽  
Vol 23 (4) ◽  
pp. 37-44
Author(s):  
SYLVESTER UWADIAE ◽  
EMIKE OMOAYENA
Keyword(s):  
Crude Oil ◽  
Soil Sample ◽  
Contaminated Soil ◽  
Polluted Soil ◽  
Soil Samples ◽  
The Other ◽  
Pseudomonas Sp ◽  
Hydrocarbon Content ◽  
Percentage Removal

This study was aimed at assessing the effectiveness of indigenous microbes for remediation of hydrocarbon contaminated soil by first increasing the population of the indigenous microbes via bioaugmentation. Soil samples were treated using a consortium of bacteria: Bacillus substilis and Pseudomonas sp. which were isolated and cultured from the contaminated soil. The non-bacteria injected soil sample had the highest hydrocarbon content (THC) of 271.021 in comparison with the other soil samples. The THC percentage removal of B1 (96.885 %), B2 (97.562 %), B3 (98.835 %), B4 (99.594 %) and B5 (99.540 %) were higher than that of the control; indicating that biodegradation actually took place.

scholarly journals Enhanced Biodegradation of Degreaser Using Pseudomonas and Bacillus Species in Fresh Water Ecosystem

2019 ◽  
pp. 1-10
Author(s):  
Renner Renner Nrior ◽  
Indutimi Mathias Otuogha
Keyword(s):  
Fresh Water ◽  
Heterotrophic Bacteria ◽  
Bacillus Species ◽  
Freshwater Ecosystem ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Microbiological Methods ◽  
Pseudomonas Species ◽  
Set Up ◽  
Hydrocarbon Utilizing Bacteria

Aim: The aim of this study is to enhance the biodegradation of degreasers used in upstream sectors of Nigeria Petroleum Industry using bio-augmenting organisms such as: Pseudomonas and Bacillus species in freshwater Ecosystem. Study Design: This study employs experimental designs, Randomized Block Design treatment set up, statistical analysis of data and interpretation. Place and Duration of Study: Freshwater sample for this research was collected from Asarama Andoni, in Rivers State, Nigeria. The study lasted for six months. Methodology: The experimental set-up was carried in 500 ml conical flask with two species of bacteria, two types of degreaser and fresh water sample giving a total of 8 set-up including controls. The Pseudomonas and Bacillus species used in this study were isolated from the freshwater ecosystem and identified using standard microbiological methods. The bioremediation potential of the respective test organisms were monitored at ambient temperature 28±0.2°C for 28 days at a constant interval of 7 days using the following Physiochemical parameter; Total dissolved Solid, Hydrogen concentrations ions and Total Hydrocarbon Content. While the following Microbiological parameters; Total heterotrophic Bacteria, Total Heterotrophic Fungi, Hydrocarbon Utilizing Bacteria, and Hydrocarbon Utilizing Fungi were monitored. Results: The percentage of degradability of the respective set-ups ranged from Control (Rigwash) (3.29%) < Pseudomonas sp. + Rigwash (27.56%) < Pseudomonas + Bacillus + Rigwash< (31.57%), Bacillus sp.+ Rigwash (37.57%) Control 2 (Aquabreak) (9.45%) < Pseudomonas sp.+ Aquabreak (26.77%) < Pseudomonas + Bacillus + Aquabreak (31.32%)< Bacillus sp.+ Aquabreak (32.46%). Overall evaluation revealed that Bacillus sp. had a higher biodegradation potential on both degreaser (Rigwash and Aquabreak) in freshwater than Pseudomonas sp. Five species of bacteria: Escherichia coli, Micrococcus, Citrobacter, Bacillus, and Pseudomonas species and four fungal species: Penicillium, Mucor, Aspergillus and Rhizopus species were isolated and identified as hydrocarbon utilizing bacteria and fungi organisms respectively. Conclusion: The results revealed that Bacillus species have more degradability potential than Pseudomonas species for both Aquabreak and Rigwash. These results also indicated the low biodegradation potential of Rigwash in fresh Ecosystem.

scholarly journals Factorial design study of total petroleum contaminated soil treatment using land farming technique

2020 ◽  
Author(s):  
Ehizonomhen Solomon Okonofua ◽  
KAYODE HASSAN LASISI ◽  
Eguakhide Atikpo
Keyword(s):  
Moisture Content ◽  
Factorial Design ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Soil Samples ◽  
Total Petroleum Hydrocarbon ◽  
Optimum Point ◽  
Input Parameters ◽  
Substrate Moisture ◽  
Land Farming

Abstract Land farming technique was used to treat hydrocarbon contaminated soil collected from a crude oil spill sites in Edo State, Nigeria. Calibrated standard auger was used to collect soil samples from the site at depth below 30 cm. The samples were characterized and classified. Cow dung and NPK fertilizer were added as additives to complement the nutriments of the soil samples before total petroleum hydrocarbon quantification and remediation procedures. Factorial design was applied to vary the input parameters such as pH, mass of substrate, moisture content and turning times of land farming so to ascertain the optimal conditions for the procedure. The result revealed that the in-situ total petroleum hydrocarbon (TPH) value was 5,000 mg kg-1 on the average and after 90 days of treatment, TPH reduced to 645.907 mg kg-1. The turning rate, pH, moisture content and mass of substrate had 82.79%, 4.36%, 0.48% and 0.046% contributions respectively to the degradation process using land farming treatment. Numerical optimization techniques applied in the optimum point for land farming input parameters to achieve predicted maximum removal of 98.60% were evaluated as pH, mass of substrate, moisture content and turning rate to be 6.01, 1 kg, 10% and 5 times in a week respectively. TPH removed at this optimum point was 97.83% reducing from 5,000 to 635.907 mg kg-1. The high coefficient of determination (r2 = 0.9865) as observed in the closeness of predicted and experimental values reflects the reliability of the model and hence, land farming practice with close attention on turning rate as revealed by this study, is recommended for TPH contaminated soil remediation.

scholarly journals Bioremediation of Petroleum hydrocarbon by using Pseudomonas species isolated from Petroleum contaminated soil

2014 ◽  
Vol 30 (4) ◽  
pp. 1771-1776 ◽  
Author(s):  
Vijay Kumar ◽  
Simranjeet Singh ◽  
Anu Manhas ◽  
Joginder Singh ◽  
Sourav Singla ◽  
...  
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Pseudomonas Species ◽  
Petroleum Contaminated Soil

scholarly journals Comunidades planctónicas y bacterianas asociadas al cultivo de bocachico Prochilodus magdalenae con tecnología biofloc

2019 ◽  
pp. 7209-7217
Author(s):  
Julia Eva Ayazo Genes ◽  
Vicente Manuel Pertuz Buelvas ◽  
César Augusto Jiménez Velásquez ◽  
José Alonso Espinosa Araujo ◽  
Víctor Julio Atencio-García ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Pseudomonas Sp ◽  
Bacillus Sp

Objetivo. Describir las comunidades planctónicas y bacterianas asociadas al cultivo de bocachico Prochilodus magdalenae con tecnología biofloc (BFT). Materiales y métodos. En nueve tanques rectangulares de concreto con volumen útil de 6.0 m3, se sembraron alevinos de bocachico con peso promedio de 1.6±0.2 g, a tres densidades 5 (T1), 10 (T2) y 20 (T3) peces/m3 con BFT, durante 120 días de cultivo. La identificación y cuantificación de los microorganismos se realizó cada ocho días, en una muestra de 250 ml de agua por tanque, mediante análisis de alícuotas en cámaras Sedgwick-Rafter y/o Neubauer bajo microscopio a 10x y 40x. Los días 15, 45 y 90 del cultivo se caracterizaron las comunidades bacterianas tomando una muestra de 2 g de floc en 90 ml de solución salina estéril y sometidas a pruebas microbiológicas convencionales. Resultados. Se identificaron cinco grupos planctónicos (microalgas, rotíferos, cladóceros, copépodos y protistas con predominancia de ciliados) con mayor cantidad de rotíferos y protistas en los cultivos con menor densidad (T1 y T2); y la mayor afluencia de microorganismos osciló entre 174.9±21.4 ind/ml (T1) y 125.6±16.1 ind/ml (T2). En el grupo de bacterias fue posible identificar 10 cepas: Escherichia coli, Enterobacter sp., Klebsiella sp., Salmonella sp. (Enterobacteriaceae) Bacillus subtilis, Bacillus sp, Lactobacillus sp, Pseudomonas sp (Vibrionaceae), Micrococcus sp, Staphylococcus sp (Cocos gram+). Conclusiones. La composición del plancton fue similar en todos los tratamientos, con rotífero y protistas como los más abundantes; la mayor proporción de bacterias fueron Enterobacterias y Heterotróficas.

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