Effect of Reducing Inflammation of Coptis chinensis Extract -Ceramide Complex through ECS Control in Atopic Dermatitis

Objectives: The purpose of this study was to confirm effect of reducing inflammation of Coptis chinensis extract -ceramide complex through the endocannabinoid system (ECS) control in atopic dermatitis.Methods: 8-week-old ICR mice were divided into normal group (Ctrl), lipid barrier elimination group (ADE), palmitoylethanolamide treated group after lipid barrier elimination (PEAT), and Coptis chinensis extract-ceramide complex applied group after lipid barrier elimination (CRA). After inducing atopic dermatitis, cannabinoid receptor (CB) 1, CB2, CD68, p-IκB, iNOS, substance P and serotonin were observed to confirm the regulation of the ECS, macrophage activity and mast cell activity.Results: CB1 and CB2 showed higher positive reactions in the CRA than in the ADE and PEAT. CD68, p-IκB and iNOS showed higher positive reaction in the ADE, PEAT and CRA than in the Ctrl, but the increase in the positive reaction was lower in the CEA compared to the ADE and PEAT. Substance P and serotonin showed higher positive reaction in the ADE, PEAT and CRA than in the Ctrl, but the increase in the positive reaction was lower in the CEA compared to the ADE and PEAT.Conclusions: The effects of Coptis chinensis extract -ceramide complex were confirmed on the regulation of the ECS, macrophage activity and mast cell activity.

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Selective Cannabinoid Receptor-1 Agonists Regulate Mast Cell Activation in an Oxazolone-Induced Atopic Dermatitis Model

Annals of Dermatology ◽

10.5021/ad.2016.28.1.22 ◽

2016 ◽

Vol 28 (1) ◽

pp. 22 ◽

Cited By ~ 15

Author(s):

Gaewon Nam ◽

Se Kyoo Jeong ◽

Bu Man Park ◽

Sin Hee Lee ◽

Hyun Jong Kim ◽

...

Keyword(s):

Atopic Dermatitis ◽

Mast Cell ◽

Cell Activation ◽

Cannabinoid Receptor ◽

Mast Cell Activation ◽

Cannabinoid Receptor 1

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The Mast Cell–SCF–CB1 Interaction Is a Key Player in Seborrheic Keratosis

Journal of Histochemistry & Cytochemistry ◽

10.1369/0022155420938031 ◽

2020 ◽

Vol 68 (7) ◽

pp. 461-471

Author(s):

Mika Yamanaka-Takaichi ◽

Koji Sugawara ◽

Rieko Sumitomo ◽

Daisuke Tsuruta

Keyword(s):

Atopic Dermatitis ◽

Mast Cell ◽

Stem Cell ◽

Stem Cell Factor ◽

Skin Diseases ◽

Cannabinoid Receptor ◽

Seborrheic Keratosis ◽

Novel Treatment ◽

Important Player

Mast cell (MC) is an important player in the development of skin diseases, including atopic dermatitis, psoriasis, and urticaria. It is reported that MC infiltration and activation are observed around various types of tumors and speculated that MCs play key roles in their pathogenesis. As MCs in human seborrheic keratosis (SK) have not been well investigated, here we focused on the MCs in SK. The number of c-Kit and tryptase-positive MCs was significantly increased around the SK compared with the marginal lesion. Degranulated MCs were also increased around the tumors. Furthermore, MC growth factor, stem cell factor (SCF), expression within the SK was significantly upregulated compared with the marginal lesion. Interestingly, one of the cognitive regulators of SCF expression, cannabinoid receptor type 1 (CB1) immunoreactivity was downregulated within the SK. Our results suggest that MCs play important roles in the pathogenesis of SK and that SCF can be also deeply involved in the development of SKs. Our current results highlight the CB1–SCF–MC interaction as a novel mechanism of SK development and this also will be utilized for developing a novel treatment.

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Mast cell-nerve fiber interaction: Ultrastructural studies

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100159680 ◽

1990 ◽

Vol 48 (3) ◽

pp. 428-429

Author(s):

E.Y. Chi ◽

M.L. Su ◽

Y.T. Tien ◽

W.R. Henderson

Keyword(s):

Mast Cell ◽

Mast Cells ◽

Substance P ◽

Nerve Fiber ◽

Sensory Nerves ◽

Ultrastructural Studies ◽

Morphological Studies ◽

Granulocyte Infiltration ◽

Intracutaneous Injection ◽

Recent Attention

Recent attention has been directed to the interaction of the nerve and immune systems. The neuropeptide substance P, a tachykinnin which is a neurotransmitter in the central and peripheral nervous systems produces tissue swelling, augemntation of intersitial fibrin deposition and leukocyte infiltration after intracutaneous injection. There is a direct correlation reported between the extent of mast cell degranulation at the sites of injection and the tissue swelling or granulocyte infiltration. It has previously been demonstrated that antidromic electrical stimulation of sensory nerves induces degranulation of cutaneous mast cells, cutaneous vasodilation and augmented vascular permeability. Morphological studies have documented a close anatiomical association between mast cells and nonmyelinated nerves, that contain substance P and other neuropeptides. However, the presence of mast cells within nerve fasicles has not been previously examined ultrastructurally. In this study, we examined ultrastructurally the distribution of mast cells in the nerve fiber bundles located in the muscular connective tissue of rat tongues (n=20).

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Therapeutic Effects of Olopatadine Hydrochloride on Blood Substance P Levels and Circulating Th17 Cell Numbers in Patients with Atopic Dermatitis

Nishi Nihon Hifuka ◽

10.2336/nishinihonhifu.72.159 ◽

2010 ◽

Vol 72 (2) ◽

pp. 159-162

Author(s):

Rieko KABASHIMA ◽

Toshinori BITO ◽

Makiko TAJIRI ◽

Chika KAWAKAMI ◽

Shoko FUKAMACHI ◽

...

Keyword(s):

Atopic Dermatitis ◽

Substance P ◽

Th17 Cell ◽

Therapeutic Effects ◽

Cell Numbers ◽

Olopatadine Hydrochloride

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Faculty Opinions recommendation of Early weaning stress induces chronic functional diarrhea, intestinal barrier defects, and increased mast cell activity in a porcine model of early life adversity.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.727683488.793540384 ◽

2017 ◽

Author(s):

Paul Enck

Keyword(s):

Mast Cell ◽

Early Life ◽

Porcine Model ◽

Cell Activity ◽

Intestinal Barrier ◽

Early Weaning ◽

Early Life Adversity ◽

Functional Diarrhea ◽

Weaning Stress

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CB1 antagonism increases excitatory synaptogenesis in a cortical spheroid model of fetal brain development

Scientific Reports ◽

10.1038/s41598-021-88750-2 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Alexis Papariello ◽

David Taylor ◽

Ken Soderstrom ◽

Karen Litwa

Keyword(s):

Brain Development ◽

Spontaneous Activity ◽

Microelectrode Array ◽

Cannabinoid Receptor ◽

Fetal Brain ◽

Endocannabinoid System ◽

Autism Spectrum ◽

Nervous System Development ◽

Inhibitory Synapses ◽

Fetal Brain Development

AbstractThe endocannabinoid system (ECS) plays a complex role in the development of neural circuitry during fetal brain development. The cannabinoid receptor type 1 (CB1) controls synaptic strength at both excitatory and inhibitory synapses and thus contributes to the balance of excitatory and inhibitory signaling. Imbalances in the ratio of excitatory to inhibitory synapses have been implicated in various neuropsychiatric disorders associated with dysregulated central nervous system development including autism spectrum disorder, epilepsy, and schizophrenia. The role of CB1 in human brain development has been difficult to study but advances in induced pluripotent stem cell technology have allowed us to model the fetal brain environment. Cortical spheroids resemble the cortex of the dorsal telencephalon during mid-fetal gestation and possess functional synapses, spontaneous activity, an astrocyte population, and pseudo-laminar organization. We first characterized the ECS using STORM microscopy and observed synaptic localization of components similar to that which is observed in the fetal brain. Next, using the CB1-selective antagonist SR141716A, we observed an increase in excitatory, and to a lesser extent, inhibitory synaptogenesis as measured by confocal image analysis. Further, CB1 antagonism increased the variability of spontaneous activity within developing neural networks, as measured by microelectrode array. Overall, we have established that cortical spheroids express ECS components and are thus a useful model for exploring endocannabinoid mediation of childhood neuropsychiatric disease.

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Therapeutic Attributes of Endocannabinoid System against Neuro-Inflammatory Autoimmune Disorders

Molecules ◽

10.3390/molecules26113389 ◽

2021 ◽

Vol 26 (11) ◽

pp. 3389

Author(s):

Ishtiaq Ahmed ◽

Saif Ur Rehman ◽

Shiva Shahmohamadnejad ◽

Muhammad Anjum Zia ◽

Muhammad Ahmad ◽

...

Keyword(s):

Cannabinoid Receptors ◽

Cannabinoid Receptor ◽

Therapeutic Potential ◽

Endocannabinoid System ◽

Cell Types ◽

Autoimmune Disorders ◽

Specific Receptors ◽

Different Cell Types

In humans, various sites like cannabinoid receptors (CBR) having a binding affinity with cannabinoids are distributed on the surface of different cell types, where endocannabinoids (ECs) and derivatives of fatty acid can bind. The binding of these substance(s) triggers the activation of specific receptors required for various physiological functions, including pain sensation, memory, and appetite. The ECs and CBR perform multiple functions via the cannabinoid receptor 1 (CB1); cannabinoid receptor 2 (CB2), having a key effect in restraining neurotransmitters and the arrangement of cytokines. The role of cannabinoids in the immune system is illustrated because of their immunosuppressive characteristics. These characteristics include inhibition of leucocyte proliferation, T cells apoptosis, and induction of macrophages along with reduced pro-inflammatory cytokines secretion. The review seeks to discuss the functional relationship between the endocannabinoid system (ECS) and anti-tumor characteristics of cannabinoids in various cancers. The therapeutic potential of cannabinoids for cancer—both in vivo and in vitro clinical trials—has also been highlighted and reported to be effective in mice models in arthritis for the inflammation reduction, neuropathic pain, positive effect in multiple sclerosis and type-1 diabetes mellitus, and found beneficial for treating in various cancers. In human models, such studies are limited; thereby, further research is indispensable in this field to get a conclusive outcome. Therefore, in autoimmune disorders, therapeutic cannabinoids can serve as promising immunosuppressive and anti-fibrotic agents.

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Treatment of atopic dermatitis using non-thermal atmospheric plasma in an animal model

Scientific Reports ◽

10.1038/s41598-021-95471-z ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Ik Jun Moon ◽

Mi Ra Yun ◽

Hae Kyeong Yoon ◽

Keon Hee Lee ◽

Sun Young Choi ◽

...

Keyword(s):

Animal Model ◽

Atopic Dermatitis ◽

Skin Barrier ◽

Treated Group ◽

Transepidermal Water Loss ◽

Atmospheric Plasma ◽

Therapeutic Modality ◽

Skin Barrier Function ◽

Control Group ◽

Epidermal Thickness

AbstractCold atmospheric plasma (CAP) has been incorporated into various fields, including promotion of cutaneous wound healing. Atopic dermatitis (AD) is a chronic cutaneous condition characterized by inflammation-induced skin wounds and impaired skin barrier function. To investigate whether CAP may improve AD using an animal model. Dermatophagoides farinae extracts (DFE)-induced murine models of AD were used in this study. The plasma-treated group received a total of 6 CAP treatments during 2 weeks, while the control group did not receive any treatment. Differences in dermatitis severity, transepidermal water loss (TEWL), serum level of immunoglobulin (Ig) E and epidermal thickness were evaluated in both groups. The dermatitis severity was significantly improved by CAP treatment. TEWL was lower in the plasma-treated group compared with the non-treated control group. Serum Ig E dropped significantly after treatment with CAP. Difference in epidermal thickness of the ear skin was not significant between the plasma-treated and non-treated groups. Localized treatment of AD with CAP decreases dermatitis severity, TEWL, and serum Ig E level. These results show CAP’s potentials as a novel therapeutic modality for AD.

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