scholarly journals Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae)

2017 ◽  
Vol 18 (4) ◽  
pp. 1430-1437
Author(s):  
ELHAM MOALLEM ◽  
ABDOLLAH GHASEMIPIRBALOUTI ◽  
TAHER NEJADSATTARI ◽  
ALIREZA IRANBAKHSH ◽  
IRAJ MEHREGAN
Keyword(s):  
Gene Expression ◽  
Medicinal Plant ◽  
Expression Analysis ◽  
Rna Extraction ◽  
Tropane Alkaloids ◽  
Expression Level ◽  
Growth Stages ◽  
Stages Of Growth ◽  
Methyltransferase Gene ◽  
Different Growth Stages

Moallem E, Ghasemipirbalouti A, Nejadsattari T, Iranbakhsh A, Mehregan I. 2017. Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae). Biodiversitas 18: 1430-1437. Physalis divaricata (Solanaceae) is one of the most prevalent weeds in summer crops. Putrescine Nmethyltransferase (PMT) is a key enzyme in the biosynthesis of nicotine, tropane alkaloids atropine, scopolamine, cocaine, and calystegines. The present study set to compare PMT gene expression during different growth stages of Ph. divaricata using RT-qPCR assay. RNA extraction was performed from root and leaf samples of a total number of 40 individuals Ph. divaricata at different growth stages (late vegetative and fruiting stages) collected from southwestern Iran. RT-qPCR of cDNA reversely synthesized from RNA was carried out using SYBR®Premix Ex TaqTM II kit. PMT gene expression levels were analyzed using ΔΔCT method. The results showed that expression level of PMT in late vegetative stage samples was significantly higher compared to fruiting stage samples. The expression level of PMT similarly changed in root and leaf samples. Direct visualization of alkaloids in different tissues using Wagner histochemical tests showed more concentrations of alkaloids in leaf idioblasts and root stele.

Gene expression analysis of peach fruit at different growth stages and with different susceptibility to Monilinia laxa

2014 ◽  
Vol 140 (3) ◽  
pp. 503-513 ◽  
Author(s):  
Michela Guidarelli ◽  
Paola Zubini ◽  
Valentina Nanni ◽  
Claudio Bonghi ◽  
Angela Rasori ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Growth Stages ◽  
Monilinia Laxa ◽  
Peach Fruit ◽  
Different Growth Stages

A new method for gene expression analysis of pure lymphocytes recovered from heterogeneous fixed mouse tissue.

2017 ◽  
Vol 35 (15_suppl) ◽  
pp. e23089-e23089
Author(s):  
Jennifer Chow ◽  
Ana Paula Galvão Da Silva ◽  
Gianni Medoro ◽  
Nicolò Manaresi ◽  
Paul David Lira ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Tissue Sample ◽  
Critical Role ◽  
Rna Extraction ◽  
Response To Treatment ◽  
Mouse Tissue ◽  
Fixed Tissue ◽  
Mouse Splenocytes

e23089 Background: Tumor infiltrating lymphocytes (TILs) are biomarkers that play a critical role in cancer diseases, including differential diagnosis, determination of prognosis, prediction of response to treatment, and evaluation of disease progression. Gene expression analysis in TILs derived from fresh tissue may not accurately depict the gene profile of the tissue microenvironment as it can change aggressively during lymphocyte isolation and RNA extraction. In addition, tissue sample size can limit the isolation of TILs with current technologies. In this study, we demonstrate the use of the DEPArray™platform to isolate pure populations of lymphocytes from a fixed mouse tissue for RNA analysi. Methods: Mouse splenocytes were activated in vitro with anti-CD3 and -CD28 for 72hs. Cells were harvested, fixed with 2% paraformaldehyde (PFA) for 20 min at RT, and stained for either CD4 or CD8 expression. Gene expression analysis of CD45, ADORA2A, GLS and GAPDH was performed in CD4+ and CD8+ DEPArray™sorted cells using the TaqMan PreAmp Cells-to-Ct kit. Results: The table below summarizes the Ct values for CD45, ADORA2A, GLS and GAPDH expression in 300 fixed unsorted control and DEPArray™sorted lymphocytes. Conclusions: We have demonstrated the feasibility of gene expression analysis on pure populations of CD4+ and CD8+ cells isolated from a fixed tissue using the DEPArray™ platform. The advantage of this approach is the DEPArray’s ability to identify and isolate subpopulations of cells from complex heterogeneous samples and/or specimens that are limited by size or content. This methodology will be applied for isolation of TILs in syngeneic and xenograft models of cancers for downstream RNA applications. [Table: see text]

scholarly journals Effects of Fis on Escherichia coli gene expression during different growth stages

Microbiology ◽  
2007 ◽  
Vol 153 (9) ◽  
pp. 2922-2940 ◽  
Author(s):  
Meranda D. Bradley ◽  
Michael B. Beach ◽  
A. P. Jason de Koning ◽  
Timothy S. Pratt ◽  
Robert Osuna
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Growth Stages ◽  
Different Growth Stages

scholarly journals The Effect of Simulated Microgravity On The Antioxidant Capacity And Alkaloid Formation In The Hyoscyamus Niger

2021 ◽  
Author(s):  
Roghayeh Pourhabibian ◽  
Alireza Iranbakhsh ◽  
Mostafa Ebadi ◽  
Halimeh Hassanpour ◽  
Azadeh Hekmat
Keyword(s):  
Gene Expression ◽  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Simulated Microgravity ◽  
Tropane Alkaloids ◽  
Mitogen Activated Protein Kinase ◽  
Hyoscyamus Niger ◽  
Pal Activity

Abstract Microgravity is one of the most important abiotic stresses in space. In the case of plant exposure to short term microgravity, plants establish strategies to response to these stresses and promote growth and survival. We hypothesized that the simulated microgravity can promote the antioxidant capacity and the formation of secondary metabolites such as tropane alkaloids in the Hyoscyamus niger. Callus induction was conducted by putting hypocotyl segments of H. niger seedlings in solid MS medium supplemented with 1 mg L−1 2,4-D and 1 mg L−1 BAP growth regulators. Then, the sub-cultured calli were placed on a clinostat for 3, 7 and 10 days. We performed Atropine and Scopolamine determination through HPLC. PAL (Phenyle alanine amonalyase) and antioxidant activity were also determined. Gene expression analysis of jasmonic acid (JA), Hyoscyamine 6-beta Hydroxylase (H6H), Putrescine N-methyltransferase (PMT), mitogen-activated protein kinase (MAPK) and ethylene responsive element binding (EREB) was performed using quantitative real time PCR. Findings showed that microgravity had a positive effect on the antioxidant capacity, Atropine and Scopolamine production in the H. niger calli. However, microgravity had a negative effect on the PAL activity. Furthermore, gene expression analysis indicated that microgravity significantly induced gene expression of the H6H, PMT and JA. It was also revealed that callus growth, carbohydrate and protein content increased in response to microgravity treatment. We conclude that microgravity can be considered as a potent factor to induce plant antioxidant activity and tropane alkaloids formation to be applicable in the pharmaceutical and medicinal industries.

scholarly journals Tumor Vascular Morphology Undergoes Dramatic Changes during Outgrowth of B16 Melanoma While Proangiogenic Gene Expression Remains Unchanged

ISRN Oncology ◽  
2011 ◽  
Vol 2011 ◽  
pp. 1-14 ◽  
Author(s):  
Elise Langenkamp ◽  
Franziska M. vom Hagen ◽  
Peter J. Zwiers ◽  
Henk E. Moorlag ◽  
Jan P. Schouten ◽  
...  
Keyword(s):  
Gene Expression ◽  
Major Change ◽  
Drug Regimen ◽  
Growth Stages ◽  
Separate Analysis ◽  
Small Vessels ◽  
Vascular Morphology ◽  
Vessel Morphology ◽  
Significant Difference ◽  
Different Growth Stages

In established tumors, angiogenic endothelial cells (ECs) coexist next to “quiescent” EC in matured vessels. We hypothesized that angio-gene expression of B16.F10 melanoma would differ depending on the growth stage. Unraveling the spatiotemporal nature thereof is essential for drug regimen design aimed to affect multiple neovascularization stages. We determined the angiogenic phenotype—represented by 52 angio-genes—and vascular morphology of small, intermediate, and large s.c. growing mouse B16.F10 tumors and demonstrated that expression of these genes did not differ between the different growth stages. Yet vascular morphology changed dramatically from small vessels without lumen in small to larger vessels with increased lumen size in intermediate/large tumors. Separate analysis of these vascular morphologies revealed a significant difference in αSMA expression in relation to vessel morphology, while no relation with VEGF, HIF-1α, nor Dll4 expression levels was observed. We conclude that the tumor vasculature remains actively engaged in angiogenesis during B16.F10 melanoma outgrowth and that the major change in tumor vascular morphology does not follow molecular concepts generated in other angiogenesis models.

scholarly journals Lifestyle Characteristics and Gene Expression Analysis of Colletotrichum camelliae Isolated from Tea Plant [Camellia sinensis (L.) O. Kuntze] Based on Transcriptome

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 782
Author(s):  
Fei Xiong ◽  
Yuchun Wang ◽  
Qinhua Lu ◽  
Xinyuan Hao ◽  
Wanping Fang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tea Plant ◽  
Growth Stages ◽  
Melanin Biosynthesis ◽  
Go Enrichment ◽  
Genetic Information Processing ◽  
Interactive Relationship ◽  
Tea Plants ◽  
Biological Process Category ◽  
Different Growth Stages

Colletotrichum camelliae is one of the most serious pathogens causing anthracnose in tea plants, but the interactive relationship between C. camelliae and tea plants has not been fully elucidated. This study investigated the gene expression changes in five different growth stages of C. camelliae based on transcriptome analysis to explain the lifestyle characteristics during the infection. On the basis of gene ontology (GO) enrichment analyses of differentially expressed genes (DEGs) in comparisons of germ tube (GT)/conidium (Con), appressoria (App)/Con, and cellophane infectious hyphae (CIH)/Con groups, the cellular process in the biological process category and intracellular, intracellular part, cell, and cell part in the cellular component category were significantly enriched. Hydrolase activity, catalytic activity, and molecular_function in the molecular function category were particularly enriched in the infection leaves (IL)/Con group. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that the DEGs were enriched in the genetic information processing pathway (ribosome) at the GT stage and the metabolism pathway (metabolic pathways and biosynthesis of secondary metabolism) in the rest of the stages. Interestingly, the genes associated with melanin biosynthesis and carbohydrate-active enzymes (CAZys), which are vital for penetration and cell wall degradation, were significantly upregulated at the App, CIH and IL stages. Subcellular localization results further showed that the selected non-annotated secreted proteins based on transcriptome data were majorly located in the cytoplasm and nucleus, predicted as new candidate effectors. The results of this study may establish a foundation and provide innovative ideas for subsequent research on C. camelliae.

The Relationship between Mutation in HOXB1 Gene and Acute Myeloid Leukemia

2019 ◽  
Author(s):  
Mohammad Yahya Vahidi Mehrjardi ◽  
Seyed Mohsen Aghaei Zarch ◽  
Mohammadreza Dehghani
Keyword(s):  
Gene Expression ◽  
Experimental Study ◽  
Acute Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Rna Extraction ◽  
Expression Level ◽  
Wild Type ◽  
Significant Difference ◽  
Heterozygous Carriers ◽  
Acute Myeloid

Background: HOX genes are an exceedingly preserved family of homeodomain-involving transcription factors. They are related to a number of malignancies, comprising acute myeloid leukemia (AML). This study aimed to evaluate the effect of HOXB1 7bp deletion mutation on HOXB1gene expression in 36 individuals. Materials and Methods: The present cross-sectional study was done on a large Iranian family. In this experimental study, 5 homozygous 7bp deletion individuals along with their unaffected siblings and their parents were investigated. The candidate gene, HOXB1 was screened and analyzed in blood samples of these participants. After RNA extraction, cDNA was synthesized according to manufacturer’s protocol. HOXB1 expression level was analyzed by 2ΔΔCT method. All laboratory procedures used in this experimental study were carried out in genetic laboratory of Shahid Sadoughi University of Medical Sciences. Results: Sequence analysis of HOXB1 gene by ABI Prism 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) revealed a family with 5 homozygous (22±17 years) and 22 healthy heterozygous carriers (42±19 years) for 7bp deletion in HOXB1 gene along with 9  healthy wild type (55±41 years). Gene expression analysis by RT-qPCR demonstrated that expression level of HOXB1 gene in wild type and heterozygous carriers specimens had similar levels (p=0.05). Conclusion: Although HOXB1 mutations has been reported in AML, but association between HOXB1 mutation and AML was not found in our study. Additionally, HOXB1 expression levels showed no significant difference between wild type and heterozygous carriers. So, HOXB1 gene expression cannot provide a powerful tool to differentiate wild type from heterozygous carries.

Sensitivity of Fieldbeans (Phaseolus vulgaris) to Reduced Rates of 2,4-D and Dicamba

Weed Science ◽  
1986 ◽  
Vol 34 (6) ◽  
pp. 953-956 ◽  
Author(s):  
Drew J. Lyon ◽  
Robert G. Wilson
Keyword(s):  
Acetic Acid ◽  
Phaseolus Vulgaris ◽  
Seed Yield ◽  
Germination Percentage ◽  
Growth Stages ◽  
Leaf Stage ◽  
Stages Of Growth ◽  
Dichlorophenoxy Acetic Acid ◽  
Different Growth Stages ◽  
Methoxybenzoic Acid

The effects of the dimethylamine salt of dicamba (3,6-dichloro-2-methoxybenzoic acid) and the dimethylamine salt of 2,4-D [(2,4-dichlorophenoxy)acetic acid] on fieldbeans (Phaseolus vulgarisL. ‘Great Northern Valley’) were studied in order to assess the potential hazards of using these herbicides in areas adjoining fieldbean production. Dicamba and 2,4-D were applied to fieldbeans at three different rates (1.1, 11.2, and 112.5 g ai/ha) and four different growth stages (preemergence, second trifoliolate leaf, early bloom, and early pod). Application of 2,4-D preemergence or in the second trifoliolate leaf stage of growth did not reduce seed yield, delay maturity, or reduce germination of seed obtained from treated plants. Dicamba or 2,4-D applied at 112.5 g/ha to fieldbeans in the early bloom or early pod stages of growth consistently reduced seed yield, delayed maturity, and reduced germination percentage. Fieldbeans exhibited a greater overall sensitivity to dicamba than to 2,4-D.

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