scholarly journals Effects of Fis on Escherichia coli gene expression during different growth stages

Microbiology ◽  
2007 ◽  
Vol 153 (9) ◽  
pp. 2922-2940 ◽  
Author(s):  
Meranda D. Bradley ◽  
Michael B. Beach ◽  
A. P. Jason de Koning ◽  
Timothy S. Pratt ◽  
Robert Osuna
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Growth Stages ◽  
Different Growth Stages

Analysis of the Crushing Effect about Ultrasound on E. coli and B. subtilis

2012 ◽  
Vol 260-261 ◽  
pp. 1017-1021
Author(s):  
Xin Ying Wang ◽  
Yong Tao Liu ◽  
Min Hui ◽  
Ji Fei Xu
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Bacillus Subtilis ◽  
Bacterial Cell ◽  
Logarithmic Phase ◽  
Growth Stages ◽  
Bacterial Cells ◽  
E Coli ◽  
Different Growth Stages ◽  
Main Factor

Escherichia coli and Bacillus subtilis as objects of the study, ultrasonic fragmentation acted on the bacterial cells in different growth stages, results showed that, it’s similar to the crushing effect of ultrasound on E. coli and B. subtilis cells of different growth stages, the highest crushing rate in the logarithmic phase, reached to 95.8% and 94.3% respectively, the crushing rate of adjustment phase is lowest, maintained at around 60%, the crushing rate stability cell was centered, which can be achieved 90%. The structure of the bacterial cell wall didn’t the main factor to decide the ultrasonic fragmentation effect, but different growth periods of bacterial cells did the determinant.

Gene expression analysis of peach fruit at different growth stages and with different susceptibility to Monilinia laxa

2014 ◽  
Vol 140 (3) ◽  
pp. 503-513 ◽  
Author(s):  
Michela Guidarelli ◽  
Paola Zubini ◽  
Valentina Nanni ◽  
Claudio Bonghi ◽  
Angela Rasori ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Growth Stages ◽  
Monilinia Laxa ◽  
Peach Fruit ◽  
Different Growth Stages

scholarly journals Tumor Vascular Morphology Undergoes Dramatic Changes during Outgrowth of B16 Melanoma While Proangiogenic Gene Expression Remains Unchanged

ISRN Oncology ◽  
2011 ◽  
Vol 2011 ◽  
pp. 1-14 ◽  
Author(s):  
Elise Langenkamp ◽  
Franziska M. vom Hagen ◽  
Peter J. Zwiers ◽  
Henk E. Moorlag ◽  
Jan P. Schouten ◽  
...  
Keyword(s):  
Gene Expression ◽  
Major Change ◽  
Drug Regimen ◽  
Growth Stages ◽  
Separate Analysis ◽  
Small Vessels ◽  
Vascular Morphology ◽  
Vessel Morphology ◽  
Significant Difference ◽  
Different Growth Stages

In established tumors, angiogenic endothelial cells (ECs) coexist next to “quiescent” EC in matured vessels. We hypothesized that angio-gene expression of B16.F10 melanoma would differ depending on the growth stage. Unraveling the spatiotemporal nature thereof is essential for drug regimen design aimed to affect multiple neovascularization stages. We determined the angiogenic phenotype—represented by 52 angio-genes—and vascular morphology of small, intermediate, and large s.c. growing mouse B16.F10 tumors and demonstrated that expression of these genes did not differ between the different growth stages. Yet vascular morphology changed dramatically from small vessels without lumen in small to larger vessels with increased lumen size in intermediate/large tumors. Separate analysis of these vascular morphologies revealed a significant difference in αSMA expression in relation to vessel morphology, while no relation with VEGF, HIF-1α, nor Dll4 expression levels was observed. We conclude that the tumor vasculature remains actively engaged in angiogenesis during B16.F10 melanoma outgrowth and that the major change in tumor vascular morphology does not follow molecular concepts generated in other angiogenesis models.

scholarly journals Lifestyle Characteristics and Gene Expression Analysis of Colletotrichum camelliae Isolated from Tea Plant [Camellia sinensis (L.) O. Kuntze] Based on Transcriptome

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 782
Author(s):  
Fei Xiong ◽  
Yuchun Wang ◽  
Qinhua Lu ◽  
Xinyuan Hao ◽  
Wanping Fang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tea Plant ◽  
Growth Stages ◽  
Melanin Biosynthesis ◽  
Go Enrichment ◽  
Genetic Information Processing ◽  
Interactive Relationship ◽  
Tea Plants ◽  
Biological Process Category ◽  
Different Growth Stages

Colletotrichum camelliae is one of the most serious pathogens causing anthracnose in tea plants, but the interactive relationship between C. camelliae and tea plants has not been fully elucidated. This study investigated the gene expression changes in five different growth stages of C. camelliae based on transcriptome analysis to explain the lifestyle characteristics during the infection. On the basis of gene ontology (GO) enrichment analyses of differentially expressed genes (DEGs) in comparisons of germ tube (GT)/conidium (Con), appressoria (App)/Con, and cellophane infectious hyphae (CIH)/Con groups, the cellular process in the biological process category and intracellular, intracellular part, cell, and cell part in the cellular component category were significantly enriched. Hydrolase activity, catalytic activity, and molecular_function in the molecular function category were particularly enriched in the infection leaves (IL)/Con group. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that the DEGs were enriched in the genetic information processing pathway (ribosome) at the GT stage and the metabolism pathway (metabolic pathways and biosynthesis of secondary metabolism) in the rest of the stages. Interestingly, the genes associated with melanin biosynthesis and carbohydrate-active enzymes (CAZys), which are vital for penetration and cell wall degradation, were significantly upregulated at the App, CIH and IL stages. Subcellular localization results further showed that the selected non-annotated secreted proteins based on transcriptome data were majorly located in the cytoplasm and nucleus, predicted as new candidate effectors. The results of this study may establish a foundation and provide innovative ideas for subsequent research on C. camelliae.

scholarly journals Dynamic changes of genomic methylation profiles at different growth stages in Chinese Tan sheep

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yufang Liu ◽  
Qiao Xu ◽  
Xiaolong Kang ◽  
Kejun Wang ◽  
Jve Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Bisulfite Sequencing ◽  
Economic Value ◽  
Growth Stages ◽  
Dynamic Changes ◽  
Genome Wide ◽  
Tan Sheep ◽  
Genomic Methylation ◽  
Different Growth Stages

Abstract Background Tan sheep, an important local sheep breed in China, is famous for their fur quality. One-month-old Tan sheep have white, curly hair with beautiful flower spikes, commonly known as “nine bends”, which has high economic value. However, the “nine bends” characteristic gradually disappears with age; consequently, the economic value of the Tan sheep decreases. Age-related changes in DNA methylation have been reported and may be responsible for age-induced changes in gene expression. Until now, no genome-wide surveys have been conducted to identify potential DNA methylation sites involved in different sheep growth stages. In this study we investigated the dynamic changes of genome-wide DNA methylation profiles in Tan sheep using DNA from skin and deep whole-genome bisulfite sequencing, and compared the DNA methylation levels at three different growth stages: 1, 24, and 48 months old (mon1, mon24, and mon48, respectively). Results In this study, 11 skin samples from three growth stages (four for mon1, four for mon24, and three for mon48) were used for DNA methylation analysis and gene expression profiling. There were 52, 288 and 236 differentially methylated genes (DMGs) identified between mon1 and mon24, mon1 and mon48, and mon24 and mon48, respectively. Of the differentially methylated regions, 1.11%, 7.61%, and 7.65% were in the promoter in mon1 vs. mon24, mon24 vs. mon48, and mon1 vs. mon48, respectively. DMGs were enriched in the MAPK and WNT signaling pathways, which are related to age growth and hair follicle morphogenesis processes. There were 51 DMGs associated with age growth and curly fleece formation. Four DMGs between mon1 and mon48 (KRT71, CD44, ROR2 and ZDHHC13) were further validated by bisulfite sequencing. Conclusions This study revealed dynamic changes in the genomic methylation profiles of mon1, mon24, and mon48 sheep, and the percentages of methylated cytosines were 3.38%, 2.85% and 4.17%, respectively. Of the DMGs, KRT71 and CD44 were highly methylated in mon1, and ROR2 and ZDHHC13 were highly methylated in mon48. These findings provide foundational information that may be used to develop strategies for potentially retaining the lamb fur and thus improving the economic value of Tan sheep.

scholarly journals Stationary-Phase Quorum-Sensing Signals Affect Autoinducer-2 and Gene Expression in Escherichia coli

2004 ◽  
Vol 70 (4) ◽  
pp. 2038-2043 ◽  
Author(s):  
Dacheng Ren ◽  
Laura A. Bedzyk ◽  
Rick W. Ye ◽  
Stuart M. Thomas ◽  
Thomas K. Wood
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Quorum Sensing ◽  
Stationary Phase ◽  
Growth Stages ◽  
E Coli ◽  
Stationary Culture ◽  
Autoinducer 2 ◽  
K 12 ◽  
Quorum Sensing Signals

ABSTRACT Quorum sensing via autoinducer-2 (AI-2) has been identified in different strains, including those from Escherichia, Vibrio, Streptococcus, and Bacillus species, and previous studies have suggested the existence of additional quorum-sensing signals working in the stationary phase of Escherichia coli cultures. To investigate the presence and global effect of these possible quorum-sensing signals other than AI-2, DNA microarrays were used to study the effect of stationary-phase signals on the gene expression of early exponential-phase cells of the AI-2-deficient strain E. coli DH5α. For statistically significant differential gene expression (P < 0.05), 14 genes were induced by supernatants from a stationary culture and 6 genes were repressed, suggesting the involvement of indole (induction of tnaA and tnaL) and phosphate (repression of phoA, phoB, and phoU). To study the stability of the signals, the stationary-phase supernatant was autoclaved and was used to study its effect on E. coli gene expression. Three genes were induced by autoclaved stationary-phase supernatant, and 34 genes were repressed. In total, three genes (ompC, ptsA, and btuB) were induced and five genes (nupC, phoB, phoU, argT, and ompF) were repressed by both fresh and autoclaved stationary-phase supernatants. Furthermore, supernatant from E. coli DH5α stationary culture was found to repress E. coli K-12 AI-2 concentrations by 4.8-fold ± 0.4-fold, suggesting that an additional quorum-sensing system in E. coli exists and that gene expression is controlled as a network with different signals working at different growth stages.

scholarly journals Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae)

2017 ◽  
Vol 18 (4) ◽  
pp. 1430-1437
Author(s):  
ELHAM MOALLEM ◽  
ABDOLLAH GHASEMIPIRBALOUTI ◽  
TAHER NEJADSATTARI ◽  
ALIREZA IRANBAKHSH ◽  
IRAJ MEHREGAN
Keyword(s):  
Gene Expression ◽  
Medicinal Plant ◽  
Expression Analysis ◽  
Rna Extraction ◽  
Tropane Alkaloids ◽  
Expression Level ◽  
Growth Stages ◽  
Stages Of Growth ◽  
Methyltransferase Gene ◽  
Different Growth Stages

Moallem E, Ghasemipirbalouti A, Nejadsattari T, Iranbakhsh A, Mehregan I. 2017. Evaluation of expression analysis of putrescine n-methyltransferase gene during different stages of growth in the medicinal plant Physalis divaricata (Solanaceae). Biodiversitas 18: 1430-1437. Physalis divaricata (Solanaceae) is one of the most prevalent weeds in summer crops. Putrescine Nmethyltransferase (PMT) is a key enzyme in the biosynthesis of nicotine, tropane alkaloids atropine, scopolamine, cocaine, and calystegines. The present study set to compare PMT gene expression during different growth stages of Ph. divaricata using RT-qPCR assay. RNA extraction was performed from root and leaf samples of a total number of 40 individuals Ph. divaricata at different growth stages (late vegetative and fruiting stages) collected from southwestern Iran. RT-qPCR of cDNA reversely synthesized from RNA was carried out using SYBR®Premix Ex TaqTM II kit. PMT gene expression levels were analyzed using ΔΔCT method. The results showed that expression level of PMT in late vegetative stage samples was significantly higher compared to fruiting stage samples. The expression level of PMT similarly changed in root and leaf samples. Direct visualization of alkaloids in different tissues using Wagner histochemical tests showed more concentrations of alkaloids in leaf idioblasts and root stele.

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