scholarly journals The morphological diversity of Chrysanthemum resulted from gamma ray irradiation

2019 ◽  
Vol 20 (2) ◽  
pp. 463-467 ◽  
Author(s):  
EMI SUSILA ◽  
ARI SUSILOWATI ◽  
AHMAD YUNUS
Keyword(s):  
Gamma Irradiation ◽  
Gamma Ray ◽  
Morphological Diversity ◽  
Leaf Length ◽  
Stem Length ◽  
Mutation Induction ◽  
Market Demand ◽  
Morphological Variations ◽  
Color Changes ◽  
Gamma Ray Irradiation

Abstract. Susila E, Susilowati A, Yunus A. 2019. The morphological diversity of Chrysanthemum resulted from gamma ray irradiation. Biodiversitas 20: 463-467. Chrysanthemum is a popular ornamental plant. The high market demand makes breeders develop new cultivars to meet market preferences. One way to get superior varieties of Chrysanthemum is by using gamma ray mutation induction which will increase the morphological variations. The purpose of this study is to determine the Chrysanthemum’s morphological diversity resulted from gamma irradiation through morphological markers. This experimental research used a completely randomized design (CRD) with one factor, i.e. radiation doses: 0 Gy (control), 10 Gy, 15 Gy, and 20 Gy. The observations were carried out qualitatively and quantitatively. The data obtained were analyzed using the SPSS 15.0 and the NTSYS (Numerical Taxonomy and Multivariate Analysis System) 2.02 program. The results showed that increasing the dose of gamma irradiation inhibited plant growth. Irradiation at 10 Gy and 20 Gy produced the most color changes in flowers compared to other doses. The basic color of 0 Gy irradiation is purple. Irradiation at 10 Gy and 20 Gy produced dark purple and deep red. Quantitative data showed that the treatment of gamma ray irradiation significantly affected the leaf length, leaf width, stem diameter, stem length, and diameter of flowers. The irradiation also caused changes in shape and texture of the leaves. The dendrogram showed that 20 Gy irradiation resulted the most diverse morphology compared to the control, 10 Gy and 15 Gy.

scholarly journals Evaluasi Keragaman Genetik Mutan Harapan Generasi MV1 Jeruk Keprok SoE (Citrus reticulata Blanco) Berdasarkan Penanda Morfologi dan ISSR

2016 ◽  
Vol 7 (2) ◽  
pp. 102
Author(s):  
Indriati Husain ◽  
Agus Purwito ◽  
Ali Husni ◽  
Kikin H. Mutaqin ◽  
Slamet Susanto
Keyword(s):  
Genetic Diversity ◽  
Gamma Irradiation ◽  
Genetic Distance ◽  
Embryogenic Callus ◽  
Irradiation Dose ◽  
Gamma Ray ◽  
Morphological Diversity ◽  
Issr Markers ◽  
Citrus Reticulata ◽  
Gamma Ray Irradiation

<p class="Abstrak"><em>ABSTRACT</em><em></em></p><p><em>Mandarin’s SoE is national <del cite="mailto:Windows%207" datetime="2016-10-31T15:54"></del>variety originated <del cite="mailto:Windows%207" datetime="2016-10-31T15:55"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins>from Mount of Mutis, Sub District of SoE, of Timur Tengah Selatan (TTS) District, East Nusa Tenggara (NTT). The genetic diversity of citrus can be induced by gamma ray irradiation on embryogenic callus cells thus producing new mutants. <del cite="mailto:Windows%207" datetime="2016-10-31T15:55"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins><del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del>Genetic diversity detection can be <ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>based on morphological and ISSR markers. The aim of this research was <del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>to obtain information on the genetic diversity <del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>on putative mutants<ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins> mandarin SoE induced by <ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>gamma ray irradiation <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del>based on morphology and markers ISSR. ISSR markers used are ISSR 1, 4, 6 and 8. Analysis of morphological diversity produced <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>a dendrogram with the level of similarity between individuals each irradiation dose 83-95% with <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>5-17% genetic distance. Dendrogram analysis based on the genetic diversity ISSR markers showed high levels of 51-100% similarity and genetic distance 0-49%. Individuals<del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del> samples obtained from<del cite="mailto:Windows%207" datetime="2016-10-31T15:58"></del><del cite="mailto:Windows%207" datetime="2016-10-31T15:58"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:58"></ins> gamma irradiation, based <ins cite="mailto:Windows%207" datetime="2016-10-31T15:58"></ins>both morphological and ISSR markers, was different from individual's genetic make up before irradiation.</em></p><p class="Abstrak"><em>Keywords</em><em>:</em><em> cluster, </em><em>gamma ray</em><em>, genetic distance, genetic diversity</em><em>s, </em><em>similarity</em></p><p class="Abstrak"> </p><p class="Abstrak">ABSTRAK</p><p>Jeruk keprok SoE adalah jeruk varietas unggul nasional yang berasal dari Pegunungan Mutis, Kecamatan SoE, Kabupaten Timur Tengah Selatan (TTS), Provinsi Nusa Tenggara Timur (NTT). Keragaman genetik jeruk ini dapat diinduksi dengan iradiasi sinar gamma pada sel-sel kalus embriogenik untuk menghasilkan mutan yang solid. Deteksi keragaman genetik yang terbentuk dapat dilakukan secara morfologi maupun molekuler dengan marka ISSR. Tujuan Penelitian ini adalah untuk mendapatkan informasi mengenai keragaman genetik yang terjadi pada mutan harapan jeruk keprok SoE hasil iradiasi sinar gamma berdasarkan morfologi dan marka ISSR. Marka ISSR yang digunakan adalah ISSR 1, 4, 6 dan 8 pada beberapa mutan harapan jeruk keprok SoE. Analisis keragaman secara morfologi menghasilkan dendrogram dengan tingkat kemiripan antar individu masing-masing dosis iradiasi 83-95% dengan jarak genetik 5-17%. Dendrogram analisis keragaman genetik berdasar marka ISSR memperlihatkan tingkat kemiripan 51-100% dan jarak genetik 0-49%. Individu-individu sampel yang diuji hasil iradiasi gamma, baik secara morfologi dan marka ISSR, telah memiliki susunan genetik yang berbeda dari susunan genetik individu sebelum diiradiasi.</p><p>Kata kunci: grup, jarak genetik, kemiripan, keragaman, sinar gamma</p>

scholarly journals Improving Performance of Coleus through Mutation Induction by Gamma Ray Irradiation

2015 ◽  
Vol 2 (1) ◽  
pp. 26-32
Author(s):  
Syarifah Iis Aisyah ◽  
Yodi Marthin ◽  
M. Rizal M. Damanik
Keyword(s):  
Gamma Irradiation ◽  
Dose Level ◽  
Gamma Ray ◽  
Lethal Dose ◽  
Mutation Induction ◽  
Coleus Blumei ◽  
Irradiation Treatment ◽  
Gamma Ray Irradiation ◽  
Number Of Leaves ◽  
Short Time

The objective of this study is to study the effect of gamma ray irradiation (15, 30, 45, and 60 gray (Gy) to determine Lethal Dose 50 (LD50) values, and to obtain new Coleus variances in a relatively short time. The study was conducted in a greenhouse at Cikabayan experimental field, Bogor Agricultural University, Darmaga, Bogor in May to July 2013. Gamma irradiation treatment significantly affected height, number of leaves, and number of nodes. Increasing the dose level of gamma irradiation tend to inhibit plant growth. LD50 for yellow/green, green/brown, variegated green/brown of Coleus blumei, and Coleus amboinicus Lour were 48.66, 65.2, 52.81, and 37.62 Gy respectively. C. amboinicus  irradiated at a dose level of 45 Gy had different leaf shapes compared to control. Keywords:  Coleus, gamma ray irradiation, LD50 values, ornamental plant, torbangun

scholarly journals Keragaan Anggrek Persilangan ♀ Vanda Celebica X ♂ Vanda Dearei Hasil Iradiasi Sinar Gamma

2017 ◽  
Vol 1 (1) ◽  
pp. 7
Author(s):  
Sri Hartati ◽  
Ahmad Yunus ◽  
Fajar Nugroho
Keyword(s):  
Genetic Diversity ◽  
Gamma Ray ◽  
Color Change ◽  
Leaf Length ◽  
Leaf Color ◽  
Color Changes ◽  
Dose Irradiation ◽  
Randomized Design ◽  
Irradiation Treatment ◽  
Gamma Ray Irradiation

<p>Variation on orchid is an advantage that allows plant as breeding material. Orchid in plant breeding has a target to increase plant genetic diversity character who favored by the consumer. Efforts to increase genetic diversity among others by artificial mutation using gamma ray irradiation. This research aims to study the influence of gamma ray irradiation dose against the performance of morphology and growth of crossbred orchids ♀Vanda celebica x ♂Vanda dearei. Research using completely randomized design single factor that is dose gamma ray irradiation 0 Gray, 10 Gray, 20 Gray, 30 Gray, 40 Gray and 50 Gray. Data were analyzed descriptively, compared plant irradiation treatments with control plants to determine the effect of irradiation on morphology and growth plant. The results showed dose irradiation treatment increased the diversity of plant height, leaf amount, leaf length, leaf color changes, the amount and length of roots. 10 Gray dose irradiation increases as height and length plant leave. 30 Gray dose irradiation increase in the number of leaves. Leaf color change occurs at 20 irradiation treatments Gray and 50 Gray.</p>

scholarly journals Effects of Gamma Irradiation on Phenotypic Changes in Vanda Hybrid

2021 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Sri Hartati ◽  
Prasetyo Prasetyo ◽  
Endang Setia Muliawati
Keyword(s):  
Gamma Irradiation ◽  
Plant Height ◽  
Gamma Ray ◽  
Ornamental Plants ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Leaf Length ◽  
Gamma Ray Irradiation ◽  
Number Of Leaves ◽  
Light Green

<p>Vanda Orchid is one of the most popular ornamental plants. Orchids have slow growth. One way to overcome this weakness is by using gamma-ray irradiation. This research aimed to study the morphological characters of various radiation doses resulting from the crossing of the Vanda Orchid. This study was carried out by observing the characters of vegetative growth for each plant on 0 Gy, 10 Gy, 20 Gy, 30 Gy, 40 Gy, and 50 Gy doses of gamma irradiation treatments. The observed variables were the increase in plant height, leaf length, root length, number of roots, number of leaves, leaf width, and leaf color. The results showed that the dose of gamma-ray irradiation were able to increase plant height. 10 Gy and 20 Gy, gamma-ray irradiation doses, resulted in discolored yellowing of orchid leaves. The discoloration of orchid leaves to light green occurs at irradiation doses of 30 Gy, 40 Gy, and 50 Gy. The morphological characteristics of <em>Vanda</em> sp. hybrid results gamma irradiation are high at dose 10-40 Gy.</p><p> </p><p>Keywords:<strong> </strong>characteristics; morphological; orchid.</p>

scholarly journals Morphological Variations in Buddleia Induced by Gamma Ray Irradiation

HortScience ◽  
2012 ◽  
Vol 47 (1) ◽  
pp. 81-83 ◽  
Author(s):  
Wenhao Dai ◽  
Victoria Magnusson
Keyword(s):  
Gamma Rays ◽  
Gamma Ray ◽  
Flower Color ◽  
Shoot Tips ◽  
Mutation Induction ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Morphological Variations ◽  
Gamma Ray Irradiation

Buddleia species is commonly used as a perennial for landscaping as a result of its heavy flowers and long bloom period. However, this species has a few concerns such as lack of flower color, excessive growth, and an invasive nature. Attempts to improve Buddleia using conventional breeding methods have resulted in limited success. In this study, mutagenesis by gamma ray irradiation was used to induce genetic variations. In vitro shoot tips of two Buddleia cultivars, B. davidii ‘Potters Purple’ and Buddleia ‘Lochinch’, were exposed to 0 to 150 Gy gamma rays and then recovered in Murashige and Skoog (MS) medium supplemented with 2.5 μM benzyladenine (BA). Shoots that recovered from the gamma ray treatment were rooted in half-strength MS medium with 0.5 μM naphthalene acetic acid (NAA) and grown in the greenhouse. The growth of shoot tips was inhibited after they were exposed to gamma rays. An average of 50.8% of shoots treated with 50 Gy gamma rays were recovered, whereas only 9.7% and 6.5% of shoots recovered when exposed to 100 and 150 Gy gamma rays, respectively. After transfer to the greenhouse, a few plants showed reduced growth with some dying before they reached the flowering stage. Various variations including characteristics of leaves (shape, size, hairs), stems (shape, internode length, branching), flowers (color, size, and structure), and plant stature were observed. This research demonstrates that in vitro mutation induction using gamma ray irradiation could be a useful protocol to develop new cultivars or genetic materials for further breeding of Buddleia or other related species.

scholarly journals ANALISIS KERAGAMAN DAN KEKERABATAN KACANG TUNGGAK (Vigna unguiculata L) GENERASI M2

2020 ◽  
Vol 5 (1) ◽  
pp. 46
Author(s):  
Yukarie Ayu Wulandari ◽  
Sobir Sobir ◽  
Syarifah Iis Aisyah
Keyword(s):  
Gamma Ray ◽  
Stem Length ◽  
Mutation Induction ◽  
High Genetic Diversity ◽  
Kinship Analysis ◽  
Group Design ◽  
Cowpea Seeds ◽  
Gamma Ray Irradiation ◽  
Putative Mutant ◽  
Number Of Seeds

Cowpea (V. unguiculata L) has great potential as a nutritious food as a substitute for soybeans because it contains sufficient protein and low fat content. The diversity of cowpea is low so that need to increase diversity through the mutation induction of gamma ray irradiation. The study was carried out in the experimental garden of Pasir Kuda PKHT of IPB in February - May 2018 using a design of augmented in the Complete Group Design in a Randomized. The study was carried out using 90 putative mutant genotypes of M2 generation as the test genotype and KM4 genotype as a comparison which was repeated 10 times. The results showed that the M2 generation putative mutants showed diversity in the qualitative and quantitative characters of cowpea. High genetic diversity is shown in the character of plant height, harvest period, number of seeds / pods and weight of cowpea seeds / plants and high broad mean heritability values obtained on the character of stem length, flowering age, number of seeds / pods and weight of beans / plant nuts arrears. The result of kinship analysis showed thirteen different putative mutant genotypes with KM4 genotypes, namely T6599P, T8028P, T7525P, T7551P, T7520P, T6574P, T6533P, T7058P, T6577P, T6591P, T7062P, T7069P and T6561.

scholarly journals Seedless fruit pummelo induced by Gamma Ray irradiation: Fruit morphological characters and stability evaluation

2018 ◽  
Vol 19 (2) ◽  
Author(s):  
BAIQ DINA MARIANA ◽  
HIDAYATUL ARISAH ◽  
YENNI YENNI ◽  
MARRY SELVAWAJAYANTI
Keyword(s):  
Gamma Ray ◽  
Pollen Viability ◽  
Embryo Sac ◽  
Morphological Characters ◽  
Market Demand ◽  
Stability Evaluation ◽  
Plant Materials ◽  
Customer Preferences ◽  
Seedless Fruit ◽  
Gamma Ray Irradiation

Mariana BD, Arisah H, Yenni, Selvawajayanti M. 2018. Seedless fruit pummelo induced by Gamma Ray irradiation: Fruit morphological characters and stability evaluation. Biodiversitas 19: 656-661. Pummelo is one of native citrus crops found in South East Asia including Indonesia. It is well known due to its big size and distinct taste compared to other citrus fruits. However, it contains many seeds, which may reduce the market demand and customer preferences. Therefore, the objective of this study was to generate seedless citrus by mutation techniques using gamma-ray radiation. The mutant induction of Pamelo Nambangan using gamma-ray radiation was conducted in 2003 using bud wood as plant materials. The bud wood was then irradiated with the dosage of 20, 40, and 60 Gy. The buds then grafted to Japansche Citroen rootstocks and maintained properly until the fruits could be observed. After three years of selection based on performance and fruit evaluation on MV2 generation, a mutant plant derived from 20 Gy irradiation treatments were obtained with improved character on the number of seeds. Pamelo Nambangan has more than 40 seeds/fruit and the mutant has less than 10 seeds/fruit on average. In the seedless mutant, it was observed that the mutant has embryo sac abortion leading to seedless fruit and low pollen viability (7.7%). The mutant has been released in 2016 as a new seedless pummelo variety under the name of ‘Pamindo Agrihorti’.

scholarly journals Effects of dose and dose rate of gamma ray irradiation on mutation induction and nuclear DNA content in chrysanthemum

2008 ◽  
Vol 58 (3) ◽  
pp. 331-335 ◽  
Author(s):  
Hiroyasu Yamaguchi ◽  
Akemi Shimizu ◽  
Konosuke Degi ◽  
Toshikazu Morishita
Keyword(s):  
Dose Rate ◽  
Dna Content ◽  
Gamma Ray ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Mutation Induction ◽  
Gamma Ray Irradiation

Destruction of 1,1-dichloroethylene/air mixture under gamma-ray irradiation

2003 ◽  
Vol 91 (5) ◽  
Author(s):  
Y.-X. Sun ◽  
T. Hakoda ◽  
A. G. Chmielewski ◽  
S. Hashimoto
Keyword(s):  
Gamma Irradiation ◽  
Gamma Ray ◽  
Batch Reactors ◽  
Radiation Induced ◽  
Gamma Ray Irradiation ◽  
Induced Decomposition

SummaryRadiation-induced decomposition of 1,1-dichloroethylene(1,1-DCE)) in air was investigated in the experiment. The model gas prepared by mixing 1,1-dichloroethylene vapor with air was sealed in batch reactors and subjected to gamma irradiation. Inorganic products (CO+CO

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