scholarly journals Effects of Gamma Irradiation on Phenotypic Changes in Vanda Hybrid

2021 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Sri Hartati ◽  
Prasetyo Prasetyo ◽  
Endang Setia Muliawati

<p>Vanda Orchid is one of the most popular ornamental plants. Orchids have slow growth. One way to overcome this weakness is by using gamma-ray irradiation. This research aimed to study the morphological characters of various radiation doses resulting from the crossing of the Vanda Orchid. This study was carried out by observing the characters of vegetative growth for each plant on 0 Gy, 10 Gy, 20 Gy, 30 Gy, 40 Gy, and 50 Gy doses of gamma irradiation treatments. The observed variables were the increase in plant height, leaf length, root length, number of roots, number of leaves, leaf width, and leaf color. The results showed that the dose of gamma-ray irradiation were able to increase plant height. 10 Gy and 20 Gy, gamma-ray irradiation doses, resulted in discolored yellowing of orchid leaves. The discoloration of orchid leaves to light green occurs at irradiation doses of 30 Gy, 40 Gy, and 50 Gy. The morphological characteristics of <em>Vanda</em> sp. hybrid results gamma irradiation are high at dose 10-40 Gy.</p><p> </p><p>Keywords:<strong> </strong>characteristics; morphological; orchid.</p>

2021 ◽  
Vol 905 (1) ◽  
pp. 012024
Author(s):  
N Salsabila ◽  
Nandariyah ◽  
E Yuniastuti ◽  
B Pujiasmanto ◽  
Sutarno

Abstract Gamma-ray irradiation on Cempo Ireng can lead to the variation of morphological characteristics; hence, morphological characterization is necessary to determine changes in potential lines. This research aimed to characterize the potential lines as one of the requirements for proposing plant variety release and add black rice genetic diversity information. The research was carried out in the rice fields of Pakahan Village, Jogonalan, Klaten in January-June 2020 with the arrangement of potential lines 8,13 and 44 of M6 in a row. In total 30 plants were selected randomly for each potential line and observed 19 morphological characters. Data analysis was carried out descriptively and qualitatively with the Chi-Square test. The results showed that gamma-ray irradiation affects the characters of leaf angle, auricle color, ligule color, leaf-blade color, leaf surface, panicle type, grain color, apiculus color, and rice length. Potential line 8 had different characteristics compared to non-irradiated Cempo Ireng in the characters of auricle color, leaf-blade color, leaf width, panicle length, grain width, rice length, and rice width, while on potential lines 13 and 44 occur in the characters of the leaf surface and panicle length.


2015 ◽  
Vol 2 (1) ◽  
pp. 26-32
Author(s):  
Syarifah Iis Aisyah ◽  
Yodi Marthin ◽  
M. Rizal M. Damanik

The objective of this study is to study the effect of gamma ray irradiation (15, 30, 45, and 60 gray (Gy) to determine Lethal Dose 50 (LD50) values, and to obtain new Coleus variances in a relatively short time. The study was conducted in a greenhouse at Cikabayan experimental field, Bogor Agricultural University, Darmaga, Bogor in May to July 2013. Gamma irradiation treatment significantly affected height, number of leaves, and number of nodes. Increasing the dose level of gamma irradiation tend to inhibit plant growth. LD50 for yellow/green, green/brown, variegated green/brown of Coleus blumei, and Coleus amboinicus Lour were 48.66, 65.2, 52.81, and 37.62 Gy respectively. C. amboinicus  irradiated at a dose level of 45 Gy had different leaf shapes compared to control. Keywords:  Coleus, gamma ray irradiation, LD50 values, ornamental plant, torbangun


2021 ◽  
Vol 250 ◽  
pp. 121-129
Author(s):  
M.I. Ivanova ◽  
◽  
A.F. Bukharov ◽  
A.I. Kashleva ◽  

Eight samples of representatives of Allium L. genus from Rhizirideum subgenus, Rhizirideum G. Don ex Koch section were studied in the conditions of the Moscow region. The presented study is planned to determine the correlation between various traits that contribute to yield increase and accumulation of biologically active compounds in leaves. Morphological characteristics (plant height, number of leaves per plant, number of shoots per plant, leaf length, leaf width and total leaf yield) and biochemical parameters (dry matter, nitrates, ascorbic acid, mono sugars, chlorophyll, carotene, flavanoids, hydroxycinnamic acids) were analyzed. Research on introduction and mobilization of genetic resources, preservation and sustention of genetic collection of representatives of Allium L. genus were carried out within the framework of the State Assignment. Dispersion analysis showed significant differences among the tested genotypes for all the studied parameters, namely phenotypic, biochemical and yield traits. The relation between the parameters was assessed using Pearson coefficient. A positive close connection was revealed between the plant height and leaf length (0.792), plant height and flavonoids (0.744), the number of shoots and the number of leaves (0.818), dry matter and nitrates (0.903), mono sugars and ascorbic acid (0.739), mono sugars and hydroxycinnamic acids (0.700), mono sugars and flavonoids (0.704), ascorbic acid and hydroxycinnamic acids (0.964), ascorbic acid and flavonoids (0.937), hydroxycinnamic acids and flavonoids (0.987). A negative close connection was noted between the height and number of shoots (-0.757), plant height and chlorophyll (-0.814), number of shoots and leaf length (-0.951), number of leaves and leaf length (-0.717), dry matter and mono sugars (-0.804 ), nitrates and mono sugars (-0.887). The revealed patterns are of interest for constructing a model of varieties and selection usage.


Pastura ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 63
Author(s):  
Prihantoro I ◽  
Anandia A. ◽  
Aryanto A. T. ◽  
Karti P. D. M. H.

Lamtoro, a legume tree plant with high protein content that has a wide range of environmental adaptation to dry stress, but relatively limited to acid stress. Mutation of lamtoro through 40 Gy gamma ray irradiation on callus culture produces lamtoro callus candidates adapted with pH 3.4. Aim of the study was to measure the morphological characteristics of adapted lamtoro cv Tarramba pH 3.4 that produced by 40Gy gamma ray irradiation based on differences of cytokinins in tissue culture. The study used complete randomized design with three treatments of different sources of cytokines i.e. kinetin, BAP and TDZ with 10 replications. The results showed that 0.5 ppm TDZ was the best treatment in callus diameter, and produced crumbly callus texture, but showed callus color response that tended to be light green.


2017 ◽  
Vol 2 (2) ◽  
pp. 21-26
Author(s):  
Arya Widura Ritonga ◽  
Dewi Sukma

Increased phenotypic diversity is needed to increase the economic value of Aglaonema. However, information on increasing phenotypic diversity of Aglaonema using gamma-ray irradiation has not been widely known. This study aimed to investigate the effect of gamma ray irradiation treatment to the performances of two varieties of Aglaonema. This research was arranged factorially using randomized group design (RKLT) of two factors consisting of 8 combinations of treatments that are 4 level of irradiation dose and 2 Aglaonema varieties. The results showed that the induction of gamma ray irradiation decreased the % viable of the plants, the number of leaves, leaf length, leaf width, and the % green color as well as increased the % blue on the leaves of Aglaonema Butterfly and Aglaonema Siam Aurora. The interaction between dose of irradiation and aglaonema varieties was obtained in the % red of leaf color. Both of Aglaonema varieties had a high radiosensitivity with LD50 values ranged of 16.70 - 17.14 Gy


2019 ◽  
Vol 20 (2) ◽  
pp. 463-467 ◽  
Author(s):  
EMI SUSILA ◽  
ARI SUSILOWATI ◽  
AHMAD YUNUS

Abstract. Susila E, Susilowati A, Yunus A. 2019. The morphological diversity of Chrysanthemum resulted from gamma ray irradiation. Biodiversitas 20: 463-467. Chrysanthemum is a popular ornamental plant. The high market demand makes breeders develop new cultivars to meet market preferences. One way to get superior varieties of Chrysanthemum is by using gamma ray mutation induction which will increase the morphological variations. The purpose of this study is to determine the Chrysanthemum’s morphological diversity resulted from gamma irradiation through morphological markers. This experimental research used a completely randomized design (CRD) with one factor, i.e. radiation doses: 0 Gy (control), 10 Gy, 15 Gy, and 20 Gy. The observations were carried out qualitatively and quantitatively. The data obtained were analyzed using the SPSS 15.0 and the NTSYS (Numerical Taxonomy and Multivariate Analysis System) 2.02 program. The results showed that increasing the dose of gamma irradiation inhibited plant growth. Irradiation at 10 Gy and 20 Gy produced the most color changes in flowers compared to other doses. The basic color of 0 Gy irradiation is purple. Irradiation at 10 Gy and 20 Gy produced dark purple and deep red. Quantitative data showed that the treatment of gamma ray irradiation significantly affected the leaf length, leaf width, stem diameter, stem length, and diameter of flowers. The irradiation also caused changes in shape and texture of the leaves. The dendrogram showed that 20 Gy irradiation resulted the most diverse morphology compared to the control, 10 Gy and 15 Gy.


2019 ◽  
Vol 20 (3) ◽  
pp. 862-866
Author(s):  
IZZATUL MUHALLILIN ◽  
SYARIFAH IIS AISYAH ◽  
DEWI SUKMA

Abstract. Muhallilin I, Aisyah SI, Dewi Sukma D. 2019. The diversity of morphological characteristics and chemical content of Celosia cristata plantlets due to gamma ray irradiation. Biodiversitas 20: 862-866. Celosia cristata L. or better known as Jengger Ayam is an ornamental plant that has a unique flower shape with attractive colors. Besides, this plant also contains antioxidants and chemical compounds that are used for traditional medicine to treat high blood pressure, and diabetes or as eye ailments. Increased diversity of C. cristata through hybridization techniques is constrained by flowers forming small grains that makes it difficult for castration and isolation of reproductive organs. This study aimed to increase C. cristata diversity of morphology and chemical content using mutation techniques induced by gamma irradiation. Plantlets were irradiated at 0, 25, 50 and 75 Gy doses. Scoring was conducted for 5 weeks until MV6 generation. Planlets were acclimatized for 3 weeks. The surviving plants were moved onto experimental land until seeded and tested for phytochemicals. Gamma irradiation with 25, 50 and 75 Gy doses produced 4 clones that have morphological changes in color and shape of the stem, leaves, and flowers. Changes in chemical content were observed with the appearance of triterpenoid compounds in 1 clone irradiated with 25 Gy.


Author(s):  
Canan Nilay Duran ◽  
Gizem Demirkaplan ◽  
Sevinç Şener

Passionflower is a member of Passifloraceae family, it can be used as medicinal and ornamental plants in addition to its consumption as fruit in the world. Guava (Psidium guajava L.) fruits, which has a rich nutrient content, are consumed both fresh and processed. Passionfruit and guava plants, which can only be cultivated in temperate southern coasts in our country, have gained commercial importance because of the fruits' export potential, high medical importance and nutrient. This study was carried out to determine the effects of some bioactivator applications on the criteria of sapling growth and development of passionflower and guava plants. The experiment was conducted between 2018-2019 under greenhouse conditions. Saplings obtained from seed germination in Akdeniz University Faculty of Agriculture Research and Experiment Area were used as plant material. 3 different commercial preparations called Messenger, Crop-set and ISR-2000 were used as bioactivators. Plant height (cm), stem diameter (mm) and number of leaves (number / plant) were measured weekly, in order to determine the effect of the applications on the growth and development of saplings. At the end of the study, it was determined that the highest average plant height (10.17 cm), stem diameter (13.53 mm) were obtained in ISR-2000 application. The highest average plant height (11.93 cm), stem diameter (16.44 mm) and number of leaves (9.07 units / plant) were obtained from Messenger application in guava plant. When the results obtained are evaluated, it is recommended that ISR-2000 bioactivator can be applied in passionflower sapling cultivation and Messenger bioactivator can be applied for guava sapling cultivation.


EUGENIA ◽  
2013 ◽  
Vol 19 (2) ◽  
Author(s):  
Ventje Pangemanan ◽  
D. S. Runtunuwu ◽  
J. Pongoh

ABSTRACT This study aimed to determine the genetic variability and heritability of morphological characters of some genotypes of potato at the Seed Station in Linelean Village, Modoinding of South Minahasa Regency. The results showed that, the relatively narrow range of genotypes variance for all the characters observed. The coefficient of variance genotypes for plant height, at 14, 42 and 56 DAP (Days After Planting) was relatively narrow, whereas at 7, 21, 28, 35 and 42 DAP relatively wide. For the character of the number of leaves at 7 and 14 DAP are wide and at 21, 28, 35, 42 and 49 DAP relatively narrow. For the character of leaf area at 7 and 14 DAP were wide and at 21, 28, 35, 42 and 49 DAP relatively narrow. For the character of the number of harvest tubers per plant and weight per tuber harvest were wide. Wide sense heritability were high for all the characters observed, except for plant height at 7 and 56 DAP were classified as moderate. Keywords: genotype variance, coefficient variance of genotype and heritability ABSTRAK Penelitian ini bertujuan untuk mengetahui variabilitas genetik dan heritabilitas karakter   morfologis beberapa genotipe kentang di Balai Benih Desa Linelean Modoinding Kabupaten Minahasa Selatan. Hasil penelitian menunjukkan bahwa ragam genotipe tergolong sempit untuk semua karakter yang diamati. Koefisien ragam genotipe untuk karakter tinggi tanaman, pada 14, 42 dan 56 HST (Hari Setelah Tanam)  tergolong sempit, sedangkan pada 7, 21, 28, 35 dan 42 HST tergolong luas. Untuk karakter jumlah daun pada 7 dan 14 HST tergolong luas dan pada 21, 28, 35, 42 dan 49 HST tergolong sempit. Untuk karakter luas daun pada 7 dan 14 HST tergolong luas dan pada 21, 28, 35, 42 dan 49 HST tergolong sempit. Untuk karakter jumlah umbi panen per tanaman dan berat per umbi panen tergolong luas. Heritabilitas arti luas semua karakter tergolong tinggi, kecuali untuk karakter tinggi tanaman pada 7 dan 56 HST yang tergolong sedang. Eugenia Volume 19  No. 2  Agustus 2013 Kata kunci: ragam genotipe, koefisien ragam genotipe dan heritabilitas


2016 ◽  
Vol 17 (1) ◽  
pp. 12 ◽  
Author(s):  
Tintin Suhartini ◽  
NFN Hadiatmi

<p>Morphological Characteristics Variability of Arrowroot (Marantha arundinaceae L.). The arrowroot has been recognized by most society member of Indonesia as a source of potential foodstuf. The arrowroot has low glicemic index, and high carbohydrate content, high quality of flour and can replace position of wheat flour as food material and industry. Evaluation and characterization are needed to get informations of superior characteristic of arrowroot as source of genetic variability to develop promising new arrowroot varieties. The result showed that the morphological characteristic of 20 arrowroot accecions were not different on the qualitative characteristics. The characteristics of leaf colour, stem and stalk leaf colour, and white colour of tuber were not different among arrowroot accecions. The quantitative characteristics of tuber or rhizomes type (tuber length and tuber circle), plant height, number of tiller/hill, total leaf/main stem, leaf length and leaf width among accecions had low variability. The tuber weight per hill had positive correlation with plant height, number of leaf, tuber length and tuber circle and negative correlation with leaf length, leaf width and stalk length leaf.</p><p> </p><p><strong>Abstrak</strong></p><p>Garut (Marantha arundinaceae L.) merupakan sumber pangan yang potensial bagi sebagian masyarakat di Indonesia. Garut memiliki indeks glikemik rendah dan kandungan karbohidrat tinggi. Tepung garut dapat menggantikan terigu sebagai bahan makanan dan industri. Evaluasi dan karakterisasi garut perlu dilakukan untuk memperoleh informasi sifat-sifat unggul untuk dapat digunakan dalam perakitan varietas unggul. Hasil evaluasi 20 aksesi garut yang dikarakterisasi menunjukan tidak ada perbedaan morfologis sifat kualitatif. Warna daun, pelepah dan tangkai daun, bentuk daun, bentuk dan warna umbi memiliki kesamaan antaraksesi. Karakter kuantitatif pada bentuk umbi (panjang dan lingkar umbi), tinggi tanaman, jumlah anakan/rumpun, jumlah daun pada batang utama, panjang dan lebar daun antar aksesi plasma nutfah garut memiliki keragaman yang sempit. Hasil analisis menunjukkan bahwa bobot umbi per rumpun berkorelasi positif dengan tinggi tanaman, jumlah daun, panjang, lingkar umbi, dan berkorelasi negatif dengan panjang daun, lebar daun, dan panjang tangkai daun.</p>


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