scholarly journals SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOLIK BUAH BUNI (Antidesma bunius (L.) SPRENG)

2016 ◽  
Vol 3 (2) ◽  
pp. 159-163
Author(s):  
Arif Rahman ◽  
Abd Malik ◽  
Aktsar Roskiana Ahmad
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Free Radical Scavengers ◽  
Bioactive Components ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Dried Fruit ◽  
Ethanol Extracts

Free radicals play a role in the occurrence of various degenerative diseases that require free-radical scavengers or antioxidants. Buni fruit (Antidesma bunius (L.) Spreng) has the bioactive components are Anthocyanins (flavonoids) that serves to the free radicals. This study aimed to measure the antioxidant activity of theethanol extract contained 70% fruit Buni obtained by using the method of nitric oxide. Simplicia buni macerated dried fruit with 70% ethanol. Extracts were obtained in the test antioxidant activity against nitric oxide radicals. The antioxidant activity against free radical absorbance measured by means of UV-Vis spectrophotometry at a wavelength of 546 nm and calcul ated IC50 values. The results shows that the fruit buni has potential as a free radical with IC50 value of 2.28 µg/mL and a comparison of quercetin with IC50 value of 5.88 µg/mL.

scholarly journals Aktivitas Antioksidan Ekstrak Metanol Daun Alpukat Menggunakan Metode DPPH

2021 ◽  
Vol 11 (2) ◽  
pp. 104-110
Author(s):  
Alfin Surya ◽  
Zaiyar Nazir ◽  
Anggun Syazulfa
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radicals ◽  
Methanol Extract ◽  
Natural Antioxidants ◽  
Positive Control ◽  
Phytochemical Screening ◽  
Bioactive Components ◽  
Ic50 Value ◽  
Ic50 Values

Free radicals are molecules that lose electrons in their outer orbitals so that the number of electrons becomes odd and unstable. Free radicals can damage various macromolecule cells including proteins, carbohydrates, fats. Antioxidants are compounds that can donate one or more electrons to free radicals. Avocado leaves contain high bioactive components that can be used as a source of natural antioxidants. Avocado leaves that have been dried, mashed and then shifted with methanol. The extracts were tested by phytochemical screening, including flavonpoid, phenolic, tannin and antioxidant activity using the DPPH (2,2-diphenyl-1-picrylhidrazyl) method. This method is based on the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) against free radicals which causes a change from purple to yellow 2,2-diphenyl-1-picrylhydrazyl) (DPPH). From the research, the IC50 values ​​in avocado leaves were 118.8056 µg / mL and 7,276 µg / mL on ascorbic acid as a positive control. From the IC50 value obtained, it is known that the methanol extract of avocado leaves has strong antioxidant activity to fight free radicals.

scholarly journals FORMULASI DAN UJI AKTIVITAS ANTIOKSIDAN KRIM EKSTRAK ETANOL DAUN MURBEI (Morus alba L.) MENGGUNAKAN METODE DPPH (1,1-Diphenyl-2-Picrylhydrazyl)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 349
Author(s):  
Eklesia Pogaga ◽  
Paulina V. Y. Yamlean ◽  
Julianri S. Lebang
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Vitamin C ◽  
Traditional Medicine ◽  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Mulberry Leaves ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Ethanol Extracts

ABSTRACTMulberry is one of the plants growing in Indonesian and widely used as traditional medicine. The active compounds found in Mulberry leaves are known have an antioxidant activity. The aim of this study is to formulate cream preparations from ethanol extracts of Mulberry leaves and to determine its antioxidant activities. Mulberry leaves was macerated using ethanol 96%. Antioxidant activity of mulberry leaves cream then evaluated against DPPH as free radical and measured at 517 nm wavelength using a UV-Vis spectrophotometer. The results showed that the cream of ethanol extract from Mulberry leaves had a very strong antioxidant activity with IC50 values in a row that is 1.7831 ppm, 0.8215 ppm and 0.7668 ppm. This can be compared with a Vitamin C solution, which has a very strong antioxidant activity with IC50 value was 1.1113 ppm. This study concludes that the cream from Mulberry leaves ethanol extract has a very strong antioxidant activity.Keywords: Mulberry leaves, Cream, Antioxidant, DPPHABSTRAK Murbei merupakan salah satu tanaman yang tumbuh di Indonesia dan  banyak digunakan dalam pengobatan secara tradisional. Kandungan senyawa aktif yang terdapat pada daun Murbei dikenal mempunyai peranan sebagai antioksidan. Tujuan dari penelitian ini ialah untuk membuat sediaan krim dari ekstrak etanol daun Murbei dan untuk menguji apakah adanya aktivitas antioksidan. Daun murbei diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Sediaan krim diuji terhadap DPPH sebagai radikal bebas dan diukur pada panjang gelombang 517 nm  dengan menggunakan spektrofotometer UV-Vis. Hasil penelitian menunjukkan bahwa krim ekstrak etanol daun Murbei memiliki aktivitas antioksidan yang sangat kuat  dengan  nilai IC50 berturut-turut yaitu 1,7831 ppm, 0,8215 ppm dan 0,7668 ppm. Hal ini dapat dibandingkan dengan larutan Vitamin C yang mempunyai aktivitas antioksidan yang sangat kuat, yaitu 1.1113 ppm. Berdasarkan hasil penelitian dapat disimpulkan bahwa krim dari ekstrak etanol daun Murbei memiliki aktivitas antioksidan yang sangat kuat.Kata Kunci: Daun Murbei, Krim, Antioksidan, DPPH

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN BUAS-BUAS (Premna serratifolia L.) ASAL KABUPATEN MELAWI PROVINSI KALIMANTAN BARAT DENGAN METODE DPPH

2020 ◽  
Vol 3 (2) ◽  
pp. 405-412
Author(s):  
Weni Puspita ◽  
◽  
Dina Yuspita Sari ◽  
Ika Ristia Rahman ◽  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Cell Damage ◽  
Antioxidant Properties ◽  
Free Radical Oxidation ◽  
The Body ◽  
Radical Oxidation ◽  
Ic50 Values ◽  
Ethanol Extracts

Buas (Premna serratifolia L.) contains secondary metabolites of flavonoids, saponins, tannins and triterpenoids/steroids, where flavonoids are known to have antioxidant properties and counteract free radicals. Antioxidants are compounds that can fight toxic hazards and reduce cell damage to the body caused by free radical oxidation. The purpose of this study was to determine the antioxidant activity of ethanol extracts of buas-buas leaves was carried out by DPPH (2,2-diphenyl-1-picrylhydrazyl), using UV-Vis spectrophotometry at wavelength 518.40 nm with various concentrations of 10 ppm, 20 ppm, 30 ppm, 40 ppm and 50 ppm. The results showed that Buas Buas leaves (Premna serratifolia L.) had an antioxidant activity with IC50 values ??of 20.66 ug / mL.

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Buah, Daun, Batang Dan Rimpang Pada Tanaman Wualae (Etlingera Elatior (Jack) R.M Smith)

2019 ◽  
Vol 5 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Asriullah Jabbar ◽  
Wahyuni Wahyuni ◽  
Muh Hajrul Malaka ◽  
Apriliani Apriliani
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Typhoid Fever ◽  
Skin Diseases ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Ethanol Extracts ◽  
Etlingera Elatior

Wualae (Etlingera elatior (Jack) R.M Smith) is one of the plants in the Zingiberaceae family, widely used in medicine ranging from rhizomes, fruits, and flowers. Empirically Wualae are usually used by people for treating skin diseases, flavoring food, a natural soap, and in Southeast Sulawesi, it is used to treat typhoid fever. This study aims to examine the antioxidant activity of Wualae (Etlingera elatior) plant. The plant extracts were extracted by maceration method, antioxidant activity test was performed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that ethanol extracts of fruits, leaves, stems and rhizomes of Wualae (Etlingera elatior) has the potential antioxidant activity with the IC50 values of 72.518 mg/L, 99.890 mg/L, 52.345 mg/L and 58.638 mg/L, respectively. and Vitamin C has IC50 value of 3,787 mg/L.

scholarly journals Antioxidant and Anti-Hyaluronidase Activities of Dragon Fruit Peel Extract and Kaempferol-3-O-Rutinoside

2019 ◽  
Vol 30 (4) ◽  
pp. 247
Author(s):  
Liana Liana ◽  
Rizal Rizal ◽  
Wahyu Widowati ◽  
Fioni Fioni ◽  
Khainir Akbar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Human Life ◽  
Fruit Peel ◽  
Paired Samples ◽  
Dragon Fruit ◽  
Ic50 Value ◽  
Significant Difference ◽  
Ic50 Values ◽  
Peel Extract

Aging is a natural process in human life and is triggered by the presence of free radicals (ROS). The use of antioxidants from natural ingredients is one of the breakthroughs to overcome aging and counteract the harmful effects caused by the free radicals. This study aimed to determine and compare the antioxidant activity of H2O2 scavenging and hyaluronidase inhibition of red dragon fruit peel extract (DFPE) and kaempferol-3-o-rutinoside (KOR) compounds. Dragon fruit peel extract (DFPE) is obtained through extraction by maceration method using 70% ethanol solvent. The design of this study included antioxidant and anti-aging activity assay of EKBN and KOR at the series concentration of 15.63; 31.25; 62.50; 125; 250; 500 µg/mL through H2O2 scavenging, as well as the DFPE and KOR hyaluronidase inhibition assay at the series concentration of 5.21; 10.42; 20.83; 41.7; 83.33; 166.67 µg/mL. EKBN shows that the average activity of H2O2 scavenging is lower than KOR. In addition, the IC50 values of KOR for H2O2 scavenging is lower (351.46±2.30ug/mL) than DFPE (409.64±23.17ug/mL). While, KOR also has higher values of inhibitory activity than of the DFPE. However, the IC50 value of KOR for hyaluronidase inhibition activity was 84.07±10.46µg/mL, equivalent to the IC50 value of DFPE (85.32±10.24µg/mL). The presence of antioxidant and anti-aging activity in the EKBN is probably caused by betalain and the KOR compound itself contained in red dragon fruit. The results of the paired-samples T-test on antioxidant activity and anti-aging of DFPE and KOR showed non-significant difference. Thus, DFPE has an equivalent antioxidant and anti-aging through H2O2 scavenging and hyaluronidase activity as possessed by the KOR compound.

Discovery of New N-hydrazinecarbothioamide Indazole Hybrids: As Potential Radical (ABTS and DPPH) Scavengers

2020 ◽  
Vol 17 (9) ◽  
pp. 1177-1185
Author(s):  
Rafaila Rafique ◽  
Arshia ◽  
Kanwal ◽  
Khalid Mohammed Khan ◽  
Sridevi Chigurupati ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radicals ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavengers ◽  
Radical Scavengers ◽  
Synthetic Compounds ◽  
Free Radical Generation ◽  
Radical Generation

Background: Free radicals are the main cause of numerous diseases. Their overproduction needs to be controlled in order to combat several ailments. The current study deals with the discovery of new free radical scavengers. Methods: Substituted N-hydrazinecarbothioamide indazoles 1-18 were evaluated for DPPH and ABTS radical scavenging activities. Results: All synthetic compounds possess good radical DPPH and ABTS scavenging potential in the ranges of IC50 = 2.11 ± 0.17 - 5.3 ± 0.11 μM and IC50 = 2.31 ± 0.06 - 5.5 ± 0.07 μM, respectively, as compared to standard ascorbic acid having IC50 = 2.02 ± 0.11 μM for DPPH and IC50 = 2.1 ± 0.07 μM for ABTS. Conclusion: These compounds could serve as leads for antioxidant activity that have the ability to control free radical generation and ward off free radical-induced disorders.

Assesment Of Antioxidant Activity Test Of KERSEN Leaf (Muntingia calabura L.) And Epiphyte with DPPH (2.2-Diphenyl-1-Picrylhidrazyl)

2020 ◽  
Vol 1 (2) ◽  
pp. 60-66
Author(s):  
Nurlutfiyyah Aini ◽  
Fatmawati ◽  
Nita Parisa
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Linear Regression Analysis ◽  
Ethanolic Extract ◽  
Morinda Citrifolia ◽  
Human Needs ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Free Radical Activity ◽  
Muntingia Calabura

Antioxidant is very important to give protection against free radical activity and highlyreactive molecules that could lead in slowing the progression of degenerative disease.In case of degenerative disease, internal antioxidant cannot neutralize the increasingconcentration of free radical. Because of that, human needs external antioxidant.Kersen (Muntingia calabura L.) is a plant that is known for its antioxidant content.Plants containing antioxidant experience is kersen (Muntingia calabura L.). Researchstudy to determine the antioxidant activity of Kersen plant and knows the differenceof antioxidant activity, based on the process of extract and infusion. Research wasdone by experimental study which was oriented in testing antioxidant activity in(Morinda citrifolia L.) extract and infusion. Extraction was done by using 96% ethanolas solvent, meanwhile infusion was made by using aquadest. Extract and infusionwas divided into group of concentration and antioxidant activity was tested byDPPH(2,2-Diphenyl-1-Picrylhidrazyl) method by measuring the absorbance usingspectrophotometer at 520 nm wavelength. Percentage of DPPH inhibition and IC50then analyzed using linear regression analysis. Ethanolic extract of kersen leaf andepiphyte had IC50 value of 113,801 ppm and 98,7802 ppm, respectively. Kersen leafinfusion showed 191,7624 ppm IC50 values, besides its epiphyte had 131,6750 ppm.Antioxidant activity of Muntingia calabura L. in the order from kersen leaf anepiphyte, and epiphyte extract has a higher antioxidant content than others.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK BAWANG HITAM (BLACK GARLIC) DENGAN VARIASI LAMA PEMANASAN

2020 ◽  
Vol 13 (1) ◽  
pp. 39-50
Author(s):  
Eva Agustina ◽  
Funsu Andiarna ◽  
Irul Hidayati
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Heating Time ◽  
The Body ◽  
Garlic Extract ◽  
Active Compounds ◽  
Rotary Evaporator ◽  
Ic50 Value ◽  
Ic50 Values ◽  
The Difference

AbstrakSenyawa radikal dalam tubuh sangat reaktif terhadap sel-sel, sehingga dapat menyebabkan berbagai penyakit. Senyawa yang mampu menangkal radikal bebas disebut antioksidan. Bawang hitam diketahui mengandung senyawa aktif fenolik dan turunannya sehingga dapat digunakan sebagai antioksidan. Penelitian tentang penggunaan bawang hitam sebagai antioksidan ini bertujuan untuk mengetahui perbedaan waktu pemanasan (15, 25, dan 35 hari) terhadap aktivitas antioksidan pada ekstrak bawang hitam. Bawang hitam diperoleh dengan cara memanaskan bawang putih selama 15, 25, dan 35 hari. Bawang hitam selanjutnya diekstraksi dengan pelarut metanol dengan metode maserasi. Filtrat hasil ekstraksi dipekatkan dengan rotary evaporator untuk mendapatkan ekstrak bawang hitam. Ekstrak bawang hitam diuji fitokimia untuk mengetahui kandungan senyawa aktif. Aktivitas antioksidan ekstrak bawang hitam ditentukan dengan metode penangkalan radikal 2,2-diphenyl-1-picrylhydrazyl (DPPH). Hasilnya menunjukkan ekstrak bawang hitam memiliki aktivitas antioksidan yang sangat kuat dengan nilai IC50 masing-masing 15 hari yaitu 2,41 µg/mL; 25 hari yaitu 2,93 µg/mL; 35 hari yaitu 2,27 µg/mL. Nilai IC50 <10 μg/mL menunjukkan bahwa ekstrak bawang hitam dengan waktu pemanasan 15, 25, dan 35 hari memiliki potensi antioksidan yang sangat kuat. Waktu pemanasan yang paling optimum dalam penangkalan radikal bebas adalah ekstrak bawang hitam dalam waktu pemanasan 35 hari, karena banyak terdapat kandungan senyawa flavonoid, tanin, sterol, dan saponin.Abstract Radical compounds in the body are very reactive to cells that can cause various diseases. Compounds that are able to ward off free radicals are called antioxidants. Black garlic are known to contain phenolic active compounds and their derivatives so that they can be used as antioxidants. Research on the use of black garlic as an antioxidant aims to determine the difference in heating time (15, 25, and 35 days) on the antioxidant activity of black garlic extract. Black garlic are obtained by heating the garlic for 15, 25, and 35 days. The black garlic is then extracted with methanol solvent by maceration method. The extracted filtrate was concentrated with a rotary evaporator to obtain the black garlic extract. Black garlic extract was tested for phytochemistry to determine the content of active compounds. The antioxidant activity of black garlic extract was determined by the radical deterrence method 2,2-diphenyl-1-picrylhydrazyl (DPPH). The results showed that the onion extract had a very strong antioxidant activity with an IC50 value of 15 days each of 2.41 µg/mL; 25 days which is 2.93 μg/mL; 35 days which is 2.27 µg/mL. IC50 values <10 μg/mL indicate that the black garlic extract with a heating time of 15, 25, and 35 days has a very strong antioxidant potential. The most optimum heating time in deterring free radicals is black garlic extract within 35 days of heating because there are many compounds containing flavonoids, tannins, sterols, and saponins.

scholarly journals Formulasi Whey Fermentasi dengan Penambahan Buah Merah (Pandanus conoideus Lam) sebagai Tabir Surya

2022 ◽  
Vol 10 (1) ◽  
pp. 28-33
Author(s):  
M. Ranggawati ◽  
I. I. Arief ◽  
Z. Wulandari
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Streptococcus Thermophilus ◽  
Lactobacillus Bulgaricus ◽  
Coarse Particles ◽  
Active Ingredients ◽  
Ic50 Value ◽  
Ic50 Values ◽  
Fruit Oil ◽  
Sunscreen Formulation

Whey produced from cheese processing can be used as non-food products such as sunscreen by fermenting using Streptococcus thermophilus and Lactobacillus bulgaricus. Adding active ingredients such as red fruit oil which contains carotenoids can increase the effectiveness of sunscreen. This study aims to determine the formulation of fermented whey and red fruit oil usage in sunscreen. The study began with the production of fermented whey and sunscreen formulations with ratios of 0, 1:1, 1:2, and 2:1 (fermented whey: red fruit oil). Furthermore, observe the organoleptic, antioxidant, SPF, pH, and homogeneity value. Adding fermented whey and red fruit oil showed no significant effect (P>0.05) onthe organoleptic test of aroma, but significantly (P<0.05) on color and texture. The antioxidant activity value test showed significantly different results (P<0.05) with IC50 values classified as moderate to high.Determination of the SPF value of sunscreen showed significantly different results  (P<0.05), but this value was classified as low protection. Although there was no significant effect on pH testing (P>0.05), the pH of sunscreen cream indicated that it was suitable for topical application. All samples showed homogeneous results and there were no coarse particles or separation between the oil and water phases. The best sunscreen formulation is 1:1 cream with the highest SPF value of 6.86 and strong antioxidant activity with an IC50 value of 56.15 g/mL. Fermented whey and red fruit oil usage as active ingredients are more effective in increasing the protection of the cream against UV rays and free radicals.

scholarly journals Uji Aktivitas Antioksidan Ekstrak Etanol Batang Daun dan Bunga Jumpai (Glinus oppositifolius (L.) Aug. DC.)

2021 ◽  
Vol 6 (1) ◽  
pp. 14
Author(s):  
Astuti Amin ◽  
Andi Paluseri ◽  
Rahmat Priyandi Linggotu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Antioxidant Potential ◽  
Activity Test ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ic50 Values ◽  
Stem Leaf

 Jumpai (Glinus Oppoitifolius (L.) Aug. DC) contains flavonoids compounds that can act as antioxidants by donating hydrogen so as to stabilize the lack of electrons in free radicals. This study aims to determine the antioxidant potential of stem, leaf, and flower extracts by looking at the IC50 value. The stems, leaves, and flowers were extracted by maceration using 70% l ethanol solvent. The results of the antioxidant activity test using the DPPH method (2,2-diphenyl-1-picrilhydrazil) showed very strong antioxidant activity with IC50 values of 9.523 µg / ml stem, 32.89 µg / ml leaves, and 23.07 µg / ml flowers with vitamin comparisons. C obtained IC50 value of 1,698 µg / ml. Based on these results, it can be rejected that the stems, leaves, and flowers have antioxidant activity with a very strong category against DPPH free radicals (2,2-diphenyl-1-picrilhydrazil). 

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