scholarly journals INVESTIGATION OF ANTIOXIDANT POTENTIAL OF QUERCETIN AND HESPERIDIN: AN IN VITRO APPROACH

2017 ◽  
Vol 10 (11) ◽  
pp. 83 ◽  
Author(s):  
Srimathi Priyanga K ◽  
Vijayalakshmi K
Keyword(s):  
Nitric Oxide ◽  
Free Radicals ◽  
High Efficiency ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Peroxide Radical ◽  
Ic50 Value ◽  
Reducing Capacity

  Objective: The study was carried out to evaluate the antioxidant potential of quercetin and hesperidin.Methods: The antioxidant potential of quercetin and hesperidin was estimated using free radicals in in vitro condition. Antioxidant activity of quercetin and hesperidin was estimated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide, nitric oxide, hydroxyl radical, hydrogen peroxide radical, and reducing power assay.Results: Quercetin and hesperidin have high efficiency in scavenging free radicals. Both the compounds are more efficient in scavenging DPPH (IC50 value 36.15±0.30 and 41.55±0.49 mg/ml) and superoxide radicals (IC 50 value 19.3±0.26 and 28.08 ±0.18 mg/ml) have high reducing capacity when compared to the standards ascorbic acid (IC50 value 31.45±1.31 and 23.2±0.20 mg/ml).Conclusion: The present study suggests that quercetin and hesperidin have significant antioxidant activities and could be easily accessible to the immune system.

scholarly journals In vitro Evaluation of Antioxidant Potential of Leaves of Opuntia dillenii Haw.

1970 ◽  
Vol 2 (1) ◽  
pp. 22-26 ◽  
Author(s):  
Raushanara Akter ◽  
SM Raquibul Hasan ◽  
Md Mokarram Hossain ◽  
Taksim Ahmed ◽  
Muntasir M Majumder ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Dependent Manner ◽  
Ic50 Value ◽  
Nitric Oxide Radical ◽  
Opuntia Dillenii

Antioxidant potential of the 80% methanol extract of the leaves of Opuntia dillenii was evaluated using 1, 1-diphenyl, 2-picryl hydrazyl (DPPH) and nitric oxide radical scavenging, reducing power, total phenol and total flavonoid content determination assays. Preliminary phytochemical screening of the extract was also carried out, which revealed that the extract possesses flavonoids, steroids, alkaloids and tannins. The extract showed significant antioxidant activities in all antioxidant assays compared to the reference antioxidant in a dose dependent manner. In DPPH radical scavenging activity, the IC50 value of the crude extract was found to be 15.71μg/mL while the IC50 value for the reference ascorbic acid was 10.84 μg/mL. Again, the extract showed remarkable nitric oxide scavenging potential and good reducing power. Moreover, the methanol extract was found to contain high amount of phenols and flavonoids, expressed as gallic acid and rutin equivalents respectively. Based on the findings of the present study, we conclude that the methanol extract of the leaves of O. dillenii possesses remarkable antioxidant potential which may be attributed to the high amount of phenols and flavonoids present in the extract. Key Words: Opuntia dillenii; Antioxidant; Reactive oxygen species; DPPH; Nitric oxide radical DOI: 10.3329/sjps.v2i1.5811Stamford Journal of Pharmaceutical Sciences Vol.2(1) 2009: 22-26

scholarly journals Screening for potential antidiabetes and antioxidant activities of selected plants from East Kalimantan, Indonesia

2019 ◽  
Vol 20 (7) ◽  
Author(s):  
RICO RAMADHAN ◽  
ALFINDA NOVI KRISTANTI ◽  
RUDIANTO AMIRTA ◽  
IRAWAN WIJAYA KUSUMA ◽  
PREECHA PHUWAPRAISIRISAN ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Medicinal Plants ◽  
Inhibitory Activity ◽  
Antioxidant Activities ◽  
Antidiabetic Activity ◽  
East Kalimantan ◽  
Ic50 Value

Abstract. Ramadhan R, Kristanti AN, Amirta R, Kusuma IW, Phuwapraisirisan P, Haqiqi MT, Saparwadi. 2019. Screening for potential antidiabetes and antioxidant activities of selected plants from East Kalimantan, Indonesia. Biodiversitas 20: 1820-1826. Ten selected plants in this study have been used traditionally in West Kutai, East Kalimantan as medicinal plants. This study aimed to screen and evaluate the in vitro antidiabetic and antioxidant activity of methanol extracts of selected plants from East Kalimantan. The antidiabetic activity was carried out by α-glucosidase inhibitory activity with maltose and sucrose as substrates while antioxidant activity was determined by free radicals scavenging activity against DPPH, ABTS and Nitric Oxide (NO). The results showed that Garcinia riedeliana extract had strong inhibitory activity with an IC50 value of 0.04 mg/mL followed by  Shorea laevis, Pternandra azurea and Bridelia tomentosa with IC50 values of 0.12; 0.15 and 0.16 mg/mL in maltose respectively, while quercetin as positive control had an IC50 value of 0.08 mg/mL. G.riedeliana extract also showed good α-glucosidase inhibitory activity with an IC50 value of 0.23 mg/mL in sucrose as substrate. The results on antioxidant activity showed that all extracts have IC50 value against free radicals DPPH, ABTS, and nitric oxide. The most active extract as α-glucosidase inhibitory activity and antioxidant activity was G. riedeliana extract. These findings provide the scientific evidence that one species of medicinal plants from East Kalimantan might be potential as an antioxidant and α-glucosidase inhibitor.                                 

Physiochemical properties and antioxidant potential of Persea Americana seed oil

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Seed Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Acid Value ◽  
Persea Americana ◽  
Antioxidant Potential ◽  
Physiochemical Properties ◽  
Physiochemical Parameters ◽  
Ic50 Value

Oil extracted from Persea Americana seed was assayed for its physiochemical properties and antioxidant potential using various standard methods. The oil content of the seed was found to be < 10%. Brownish-red color oil was liquid at room temperature, with specific gravity of 0.91±0.02 g/mL. Other physiochemical parameters determined were; acid value (4.51±0.08 mgKOH/g), %FFA (2.26±0.08), peroxide value (2.40±0.57 mgO2/Kg), ester value (31.26±0.03 mgKOH/g), saponification value (35.76±0.07 mgKOH/g) and iodine value (23.5±0.07). The results of the antioxidant activities of the seed oil showed that the flavonoid content (80.00±1.41 mgQE/g) was ~10 folds higher than the phenolic content (8.27±0.06 mgGAE/g). The DPPH radical scavenging value was found to be 51.54±0.25% with an IC50 value of 4.68±0.02 mg/mL and reducing power with an average absorbance of 0.85±0.01 and an IC50 value of 0.001±0.02 mg/mL. Gallic acid showed better antioxidant activities than the oil studied. The results obtained in this study showed that Persea Americana seed oil has nutritional, industrial as well as medicinal potentials.

Zawartość związków fenolowych w ekstrakcie z propolisu oraz ocena jego aktywności przeciwutleniającej i cytoochronnej względem erytrocytów ludzkich w warunkach stresu oksydacyjnego in vitro

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Agnieszka Waśkiewicz ◽  
Marta Babicka ◽  
Elżbieta Hołderna-Kędzia ◽  
...  
Keyword(s):  
Free Radicals ◽  
Phenolic Acids ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Human Erythrocytes ◽  
Antioxidant Potential ◽  
Natural Material ◽  
Propolis Extract

Introduction. Propolis (bee glue) is a natural product collected by honeybees from buds of various trees, shrubs and other plant species. Extracts of propolis possess numerous biological activities, including antioxidant, antibacterial, antifungal and anticancer. For this reason, propolis is currently used in many applications, such as preparations for cold syndrome, dermatological preparations or as a constituent of nutritional supplements and health food. The chemical composition of this natural material is very complex and depending on many factors, including method of extraction and selection of the solvent for the extraction process. Aim. The aim of the study was to determine concentration of selected phenolic compounds (flavonoids and phenolic acids) in extract of Polish propolis and estimate its antioxidant activity and effect on human red blood cells. Material and methods. In the propolis extract was determined concentration of 14 flavonoids and 9 phenolic acids using ultra-performance liquid chromatography equipped with a photodiode detector and a triple quadrupole mass spectrometer. The antioxidant potential of propolis extract was evaluated applying DPPH˙ free radical scavenging activity assay and Fe3+ reducing power assay. Moreover, the cytotoxicity and cytoprotective potential of propolis extract was estimated using human erythrocytes in vitro. Results. The propolis extract contained high concentration of pinocembrin, galangin, chrysin, apigenin, kaempferol, coumaric acid and cinnamic acid. It exhibited also high antioxidant potential. The antiradical activity of examined propolis extract was equal to 75% approx. activity of both standard antioxidants used in the study, namely Trolox and BHT. The reducing power of extract was equal to 65% approx. of Trolox and 80% of BHT, respectively. The propolis extract had no hemolytic activity, moreover, effectively protected human erythrocytes against free radicals-induced damage in vitro. Conclusions. The results of this study indicate that the propolis extract of national origin is a rich source of flavonoids and phenolic acids. Therefore, the propolis extract possesses a high antioxidant potential and can protect erythrocytes against free radicals-induced oxidative hemolysis.

scholarly journals Phytochemical analysis using GC-FID, FPLC fingerprinting, antioxidant, antimicrobial, anti- inflammatory activities analysis of traditionally used Eucalyptus globulus essential oil

2021 ◽  
Vol 5 (1) ◽  
pp. 26-38
Author(s):  
Arun Dev Sharma ◽  
Mohit Farmaha ◽  
Inderjeet Kaur ◽  
Narveer Singh
Keyword(s):  
Nitric Oxide ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Fluorescent Spectroscopy ◽  
Ft Ir ◽  
Pharmaceutical Industries ◽  
Anti Inflammatory ◽  
Eucalyptus Globules

Eucalyptus globules is an widely distributed in tropical and subtropical regions. It has been widely used as folk medicine, and folk cosmetic owing to its antioxidant values. Despite its importance, phytochemical and pharmacological studies remain infancy. This study was aimed at extraction of essential oil by steam-distillation and evaluation of bioactive components, antioxidant, antimicrobial, anti-inflammatory activities along with analysis by UV-VIS, FT-IR and Fluorescent techniques. Fast protein liquid chromatography (FPLC) was used to confirm the presence of polyphenols. Different antioxidant activities like DPPH., ABTS.+, .OH, superoxide, nitric oxide and reducing power of the essential oil. Essential oil was analyzed by UV-VIS, FT-IR and Fluorescent techniques. In vitro antimicrobial activity was also monitored. FT-IR fingerprint qualitative analysis was performed using commercial standards. Considerable amount of flavonoids were detected in essential oil.  Oil exhibited considerable scavenging activities of ABTS.+, .OH, superoxide, nitric oxide and reducing power. UV-VIS, FT-IR analysis revealed the presence of polyphenolics in essential oil. Fluorescent spectroscopy revealed the presence of fluorophores in essential oil. FPLC and FT-IR fingerprint analysis revealed the presence of bioactive constituents like rutin, tannic acid, vanillic acid and ascorbic acid in the essential oil. A strong anti-inflammatory activity of oil was observed using fluorescent spectroscopy. An appreciable in vitro antibacterial activity against gram-negative bacteria like Acetobacter aceti and Pseudomonas aeruginosa was detected. The data provides the scientific support to the use of essential oil from Eucalyptus globules as a potent herbal source of bioactive compounds possessing natural antioxidant activities in food and pharmaceutical industries.

scholarly journals Evaluation of the antioxidant activity of various extracts of aerial parts of Cassia absus: An in-vitro techniques

2020 ◽  
Vol 11 (2) ◽  
pp. 1545-1550
Author(s):  
Mythri M ◽  
Sanal Dev K T ◽  
Kottai Muthu A
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Potential ◽  
In Vitro Techniques ◽  
Aerial Parts ◽  
Ic50 Value ◽  
Total Antioxidant ◽  
The Difference

Cassia absus (Linn)Cassia absus(Linn) (family Fabaceae ) is generally known as “chaksu ” inan ayurvedic traditional system.The current study,aerial parts of different concentrates(Pet.ether, ethyl acetate and methanol) of Cassia absus, was evaluated for its in-vitro antioxidant potential byDiphenylpicrylhydrazyl radical,nitric oxide activity andtotal antioxidant activitytaking ascorbate as the standardfor all the three methods. The IC50 value was originated that methanolic concentrates of Cassia absusmore efficient inDiphenylpicrylhydrazyl radical,nitric oxide activity, total antioxidant activitycompared EA&PEconcentrates.The methanolic concentrates of Cassia absus& ascorbic acid exhibited antioxidant potential possessing IC50230µg/ml &130µg/ml (Nitric oxide). 205µg/ml &57µg/ml (total antioxidant),195µg/ml & 66µg/ml (Diphenylpicrylhydrazyl radical)respectively. The difference in the scavenging potential of the extracts can be due to variation in the percentage of bioactive compounds present in different solvents. Invitroantioxidant studiesobviouslyshow the methanolic concentrates of Cassia absushave better antioxidant activity. This result indicates that aerial parts of methanolic concentratesCassia absuscould serve as a natural antioxidant, which may be useful in preventfree radical-induced diseases.

scholarly journals Comparative Antioxidant Potential of Different Extracts of Celastrus paniculatus Willd. Seed

1970 ◽  
Vol 3 (1) ◽  
pp. 68-74 ◽  
Author(s):  
Fatema Tuz Zohera ◽  
Md Razibul Habib ◽  
Mohammad Zafar Imam ◽  
Md Ehsanul Hoque Mazumder ◽  
Md Sohel Rana
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Dependent Manner ◽  
Ic50 Value ◽  
Celastrus Paniculatus

The objective of the present study was to evaluate the comparative antioxidant potential of methanol, ethyl acetate, pet ether and water extracts of Celastrus paniculatus seed. Antioxidant activity was evaluated by using total phenol and flavonoid content determination assays, total antioxidant capacity, 1,1-diphenyl-2- picryl-hydrazil (DPPH) free radical assay, Reducing power assessment, Nitric oxide (NO) scavenging assay and Cupric ion reducing capacity assay (CUPRAC method). The extracts showed moderate antioxidant activity in a dose dependent manner. The extracts were found to contain phenolics and flavonoid compounds. In DPPH radical scavenging assay, ethyl acetate extract had the lowest IC50 value (585.58μg/ml) compared to ascorbic acid. In nitric oxide scavenging assay IC50 value was found to be 122.99μg/ml, 320.54μg/ml, 601.81μg/ml and 206.37μg/ml respectively for the Water, Methanol, Ethyl Acetate and Pet Ether extracts compared to 6.83μg/ml which was the IC50 value for the reference ascorbic acid. The extracts also showed good reducing power. The results of the present study indicate that the extracts possesses significant antioxidant potential of which ethyl acetate extract is the most promising one and possess highest antioxidant potential. Key Words: Celastrus paniculatus; antioxidant; DPPH; NO scavenging; CUPRAC; ROS. DOI: 10.3329/sjps.v3i1.6802S. J. Pharm. Sci. 3(1): 68-74

In vitro propagation of Caralluma tuberculata and evaluation of antioxidant potential

Biologia ◽  
2014 ◽  
Vol 69 (3) ◽  
Author(s):  
Riaz Rehman ◽  
Muhammad Chaudhary ◽  
Khalid Khawar ◽  
Gang Lu ◽  
Abdul Mannan ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Large Scale ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Ms Medium ◽  
Scavenging Activity ◽  
Induction Rate

AbstractPresent study describes rapid in vitro propagation of Caralluma tuberculata, a traditional medicinal plant, and antioxidant potential of calli and plants extracts. The highest callus induction rate (93.3%) with maximum weight of calli 5.2 g was achieved from shoot tip explants on MS medium supplemented with 9.04 μM 2,4-D and 4.44 μM BA. The maximum shoot induction rate (71.1%) with mean number of shoots 3.66 ± 1.53 and 4.6 cm average shoot length was observed on 13.32 μM BA, 4.52 μM 2,4-D and 2.89 μM GA3 appended in MS medium. The developed shoots were best rooted in the presence of 5.07 μM IAA with 3.0 ± 0.15 roots per plantlet. The plants were successfully acclimatized under in vivo conditions. The plants and calli extracts exhibited good antioxidant activities, however, plant extract activities were more pronounced. The phenolic compounds in plant and calli extracts were 0.16% and 0.057%, respectively. While the flavonoids were 0.092% in plant and 0.039% in calli extract. Total Phenolics, flavonoids; DPPH radical scavenging activity and reducing power potential distributed among different fractions depending upon polarity of the solvent. The highest DPPH scavenging activity and reducing power was exhibited by water fractions; 4.95 mg/mL and 0.729 OD at 10 mg/mL, respectively. The micropropagation protocol can be successfully used for large-scale multiplication and conservation of germplasm of this threatened plant. Furthermore, antioxidant value describes importance of this valuable plant as food and medicine.

scholarly journals In vitro Antioxidant Potential of Abrus precatorius L. and Asystasia gangetica (L.) T. Anderson

2021 ◽  
Vol 11 (4) ◽  
pp. 4071-4079
Keyword(s):  
Antioxidant Activities ◽  
Human Life ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Peroxide Radical ◽  
Medicinal Uses ◽  
Abrus Precatorius ◽  
Asystasia Gangetica

Antioxidant substances are an important part of human life as it plays a key role in nutraceuticals and also help to defend free radicals present in our body. The present study highlights the need to find potent natural antioxidants from medicinal plants. Different extracts of Abrus precatorius L. (Fabaceae) and Asystasia gangetica (L.) T. Anderson (Acanthaceae) were successively prepared using petroleum ether, benzene, chloroform, and ethanol. It was evaluated for antioxidant activities using various assays. Maximum extractable total phenolics and flavonoids were recorded in varied extracts of A. precatorius and A. gangetica. The extracts also showed efficient phosphomolybdenum reduction, reducing power activity, nitric oxide, and hydrogen peroxide radical scavenging properties. It is very clear from the results that the studied plants A. precatorius and A. gangetica have remarkable medicinal uses with extraordinary potential for pharmaceuticals. Further detailed studies will pave the way to promote natural drugs for health benefits.

In vitro Antioxidant potential, Total Phenolic and Flavonoid contents in Justicia gendarussa leaf extracts

2021 ◽  
pp. 2707-2713
Author(s):  
Ramya Kuber B
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Free Radicals ◽  
Defense Mechanism ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Neutralizing Capacity

Free radicals are triggered in human body by routine metabolic pathways which are highly reactive substance entangled in various physiological functions but excess production may impart with multitude diseases such as cancer, arthritis, inflammation and various neurodegenerative disorders. As a result, previous findings supported that various photochemical constituents present in natural plants exerts prominent role in defense mechanism against rate of excess free radicals’ production. The present study was carried out to investigate phytochemical composition, quantification studies and in vitro antioxidant potential of Justicia gendarussa (JG) various solvent extracts. JG belongs to the family Acanthaceace and used as traditional healers in various ailments. JG plant material was collected, dried, powdered and subjected for soxhlation to prepare ethanolic, ethyl acetate, aqueous and n-hexane extracts. Screening of Active constituents by various qualitative tests, estimation of total phenolic (TPC) and Total flavonoid content (TFC) and in vitro antioxidant potential of plant extracts were performed by DPPH, Lipid peroxidation, Nitric oxide and Superoxide radical scavenging methods. Present investigation reveals that, presence of flavonoids, phenols, amino acids, glycosides, cardiac glycosides, proteins, carbohydrates, alkaloids, steroids, terpenoids and tannins. TPC and TFC of ethanol extract were found to be 9.47 ± 0.0216 mg Gallic acid equivalent (GAE/g) and 97.6 ± 0.0342 mg Rutin equivalent/g (RUE/g). Among all the analyzed extracts ethanolic extract of JG possessed high radical neutralizing capacity and for DPPH (IC50 32µg/ml), Lipid Peroxidation (28 µg/ml), Nitric oxide (30 µg/ml) and superoxide anion radicals (39 µg/ml) respectively and found to be more effective as ascorbic acid (26 µg/ml) which was used as standard. Overall results of the study concluded that JG leaf extracts possess beneficial Phytoconstituents, high phenol and flavonoid contents and potential antioxidant activity, which could be a viable source of natural antioxidants for treating various degenerative disorders.

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