Characterization and expression of Cm-AAT1 gene encoding alcohol acyl-transferase in melon fruit (Cucumis melo L.) ‘Hikapel’

Abstract. Wibowo WA, Fatkhurohman MI, Daryono BS. 2020. Characterization and expression of Cm-AAT1 gene encoding alcohol acyl-transferase in melon fruit (Cucumis melo L.) ‘Hikapel’. Biodiversitas 21: 3041-3046. Melon (Cucumis melo L.) is one of the horticulture commodities that have high economic value and its needs increase continuously. Many new melon cultivars have been assembled to produce a higher quality melon. Melon 'Hikapel' developed by the Laboratory of Genetics and Breeding, Faculty of Biology UGM has distinctive character in the form of a strong aroma. This aroma is a complex mixture of various kinds of volatile compound. One of the main determinant compounds is a volatile ester, synthesized by the alcohol acyl-transferase enzyme encoded by the Cm-AAT1 gene. Characterization of Cm-AAT1 began with isolation of melon rinds to get total RNAs. Synthesis cDNA was conducted with oligo-dT primer, followed by detection of Cm-AAT1 using specific primers. A specific band was sequenced to perform phylogenetic tree. Gene expression from 4 melon cultivars, ‘Hikapel’, ‘Hikadi’, ‘Sun Lady’, and ‘Luna’ analysis was performed using relative quantitative Real-Time PCR. The results of this study showed that Cm-AAT1 owned not only by aromatic cultivars ‘Hikapel’ and ‘Hikadi’, but also owned by non-aromatic cultivars ‘Sun Lady’ and ‘Luna’. Phylogenetic analysis shows a high similarity between Cm-AAT1 on 'Hikapel' and 'Hikadi'. Gene expression analysis on 'Hikapel' increases as the process of fruit ripening during the storage period and it is in contrast to 'Hikadi' at decrease when the fruit began to enter the decay process on day 7th. Expression of Cm-AAT1 on ‘Hikapel’ was higher than ‘Hikadi’ at the peak of fruit maturity.

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Cloning and characterization of the gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase in melon (Cucumis melo L. reticulatus)

Molecular Genetics and Genomics ◽

10.1007/s004380000401 ◽

2000 ◽

Vol 265 (1) ◽

pp. 135-142 ◽

Cited By ~ 31

Keyword(s):

Cucumis Melo ◽

Coenzyme A ◽

Gene Encoding ◽

Cucumis Melo L ◽

Coenzyme A Reductase

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The use of postharvest treatments to extend storage life and to control postharvest wastage of honeydew melons (Cucumis melo L. var. inodorus Naud.) in cool storage

Australian Journal of Experimental Agriculture ◽

10.1071/ea9900693 ◽

1990 ◽

Vol 30 (5) ◽

pp. 693 ◽

Cited By ~ 7

Author(s):

ME Edwards ◽

RM Blennerhassett

Keyword(s):

Water Loss ◽

Cucumis Melo ◽

Storage Temperature ◽

Chilling Injury ◽

Storage Period ◽

Storage Conditions ◽

Storage Life ◽

Storage Rots ◽

Wax Coating ◽

Cucumis Melo L

Three trials were undertaken to study storage conditions and handling procedures required to maximise the postharvest storage life of honeydew melons (Cucumis melo L. var. inodorus Naud.).Honeydew melons treated with chlorine (1000 mg/L), benomyl (250 mg/L) + guazatine (500 mg/L), shrink wrap (17 ym Cryovac XDR film), Semperfresh, wax, or combinations of these treatments were stored at 4 or 8�C, for 4 or 6 weeks. Benomyl plus guazatine reduced the development of storage rots associated with Alternaria and Fusarium spp. The use of shrink wrap and wax reduced water loss by melons but increased fungal infection in some cases. Shrink wrapping combined with the fungicide treatment effectively reduced the incidence of fungal breakdown in the storage period for up to 4 weeks. Wax coating with full strength Citruseal wax caused anaerobic tissue breakdown. Melons were affected by chilling injury at 4�C. Control of bacterial rots with benomyl + guazatine or with chlorine was variable. Semperfresh did not reduce the incidence of fungal breakdown or water loss from the melons. The results indicate that storage of honeydew melons for 4 weeks at 8�C by pretreating with fungicide is possible but the melons soften and rot after 6 weeks, making them unsaleable. Four weeks should be adequate to allow for sea freighting of honeydew melons to markets in South East Asia. Further research is required to determine the optimum storage temperature for honeydew melons.

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STIMULASI HASIL MELON ( Cucumis melo, L) DENGAN MENGGUNAKAN BIOTO GROW GOLD (BGG)

Jurnal Ilmiah Pertanian ◽

10.31849/jip.v15i1.1481 ◽

2018 ◽

Vol 15 (1) ◽

pp. 35-41

Author(s):

SAHINDAH ARITONANG ◽

SURTINAH SURTINAH

Keyword(s):

Data Analysis ◽

Cucumis Melo ◽

Sugar Content ◽

Fruit Weight ◽

Leaf Length ◽

Stem Diameter ◽

Leaf Width ◽

Randomized Design ◽

Cucumis Melo L ◽

Melon Fruit

The experiment was conducted experimentally using non-factorial Randomized Design (RAL) with 5 (five) treatment levels without Bioto Grow Gold, Bioto Grow Gold 1 ml liter-1 water, Bioto Grow Gold 2 ml liter -1 water, giving Bioto Grow Gold 3 ml liter-1 water and giving Bioto Grow Gold 4 ml liter-1 water. The data analysis used variance and continued with a different test of Duncan treatment average at p 0.05. The results showed that BGG treatment had the significant effect on leaf length, leaf width, stem diameter, flowering age, fruit circumference, fruit weight, a thickness of flesh and sugar content of melon fruit. The best treatment is giving Bioto Grow Gold 3 ml liter-1 water.

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Influence of storage period and temperature on the postharvest characteristics of six melon (Cucumis melo L., Inodorus Group) cultivars

Postharvest Biology and Technology ◽

10.1016/0925-5214(94)00024-m ◽

1995 ◽

Vol 5 (3) ◽

pp. 211-219 ◽

Cited By ~ 29

Author(s):

Vito Miccolis ◽

Mikal E Saltveit

Keyword(s):

Cucumis Melo ◽

Storage Period ◽

Cucumis Melo L

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CmBGI Gene Expression encoding β-glucosidase in melon (Cucumis melo L.) under stress condition

BIOTROPIC The Journal of Tropical Biology ◽

10.29080/biotropic.2017.1.2.1-8 ◽

2017 ◽

Vol 1 (2) ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Yuanita Rachmawati ◽

Ganies Rizaa Aristya ◽

Budi Setiadi Daryono

Keyword(s):

Gene Expression ◽

Normal Condition ◽

Cucumis Melo ◽

Stress Condition ◽

Leaf Tissue ◽

Adaptive Responses ◽

Degraded Land ◽

Biotic Stresses ◽

Aba Metabolism ◽

Cucumis Melo L

CmBGI is the enzymatic genes encoding β-glucosidase that involved in Abscisic Acid (ABA) metabolism of Cucumis melo L. β-glucosidase promotes the accumulation of glucose, fructose, and sucrose, and it might act as a regulator that mediates melon fruit ripening both climacteric and nonclimacteric. ABA mediates adaptive responses to abiotic and biotic stresses. Agricultural Balitbang in 1997 showed that there were approximately 158.600 ha of degraded land scattered in three zones of agroecosystems in Yogyakarta (DIY). One of them is Dlingo Bantul area which has a karst type critical land area. Karst provides stress to the certain plant growth. One way to conserve critical land is making this area for agriculture. Cultivar TACAPA and TA were superior melons that have been developed by Genetic Laboratory of Biology Faculty UGM. This preliminary research was conducted to examine molecular characterization of CmBGI gene expression in cultivar TACAPA and TA which are planted in normal condition medium and in critical land medium treatment. Total RNA was extracted from leaf tissue then Reversed Transcriptase (RT-PCR) to collect cDNA library. cDNA was amplified using specific primer. Spectrophotometry was conducted in λ260 nm and electrophoresis run in 1.5% agarose gel. Control of band chosen was Cm-Actin. CmBGI gene concentration of TACAPA and TA in normal condition medium are in succession 578.5 and 579.4 μg/ml then for critical land medium treatment 743.4 and 773.5 μg/ml. CmBGI band was showed both of TACAPA and TA as ± 1258 bp. Cm-actin was showed band of DNA as ± 445 bp. CmBGI gene concentration in critical land medium treatment which is given greater stress on melons are higher than normal condition. This suggests that the CmBGI gene is expressed more in cultivar TACAPA and TA melons when they are grown under stress condition.

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