scholarly journals Chemical composition diversity of fucoidans isolated from three tropical brown seaweeds (Phaeophyceae) species

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
FADILAH NOR LAILI LUTFIA ◽  
Alim Isnansetyo ◽  
Ratna Asmah Susidarti ◽  
Muhammad Nursid
Keyword(s):  
Chemical Composition ◽  
Uronic Acid ◽  
Dry Matter ◽  
Deae Cellulose ◽  
Brown Seaweed ◽  
Exchange Chromatography ◽  
Chemical Components ◽  
Brown Seaweeds ◽  
Total Carbohydrates ◽  
High Sulfate

Abstract. Lutfia FN, Isnansetyo A, Susudarti RA, Nursid M. 2020. Chemical composition diversity of fucoidans isolated from three tropical brown seaweeds (Phaeophyceae) species. Biodiversitas 21: 3170-3177. Fucoidan is a polysaccharide with high sulfate content, found in the cell walls matrix of brown seaweed. Its bioactivities vary depending on the algae species and the chemical structure. The aims of this research were to extract, purify, and characterize fucoidan from three Indonesian brown seaweed, Sargassum sp., Turbinaria sp., and Padina sp. The extraction was carried out using acid method followed by precipitation with ethanol and CaCl2, while its purification using DEAE-cellulose ion-exchange chromatography. Characterization was performed by FTIR and 1H-NMR spectroscopic analyses. Chemical components of fucoidan determined were total carbohydrates, sulfate residue, uronic acid residue, and monosaccharide components. The results revealed that the Turbinaria sp. produced highest yield of fucoidan (4.8% dry matter), followed by Sargassum sp. (2.7% dry matter) and Padina sp. (2.6% dry matter). The carbohydrate contents of Sargassum sp., Turbinaria sp. and Padina sp. fucoidan were 64.55±0.12%, 67.42 ± 0.03% and 62.90 ± 0.04% with sulfate residues of 0.013% ± 4.71; 19.47±0.002% and 0.016%±8.81; and uronic acid residues of 25.19±0.03%; 12.69±0.03% and 12.91±0.01%, respectively. Sargassum sp., Turbinaria sp., and Padina sp. fucoidans consist of fucose and some other minor monosaccharides.

Separation of Heparin into Subtractions by DEAE-Cellulose Chromatography

1979 ◽  
Vol 42 (05) ◽  
pp. 1452-1459 ◽  
Author(s):  
Robert H Yue ◽  
Toby Starr ◽  
Menard M Gertler
Keyword(s):  
High Activity ◽  
Uronic Acid ◽  
Deae Cellulose ◽  
Exchange Chromatography ◽  
Net Charge ◽  
Uronic Acid Content ◽  
Successful Separation ◽  
Salt Gradient ◽  
Structure And Activity ◽  
Low Activity

SummaryCommercial porcine heparin can be separated into three distinct subtractions by using DEAE-cellulose chromatography and a stepped salt gradient. Gram quantities of heparin can be fractionated by this technique. All three heparin subtractions can accelerate the inhibition of thrombin by antithrombin III with different efficiency. The specific activities of the high activity heparin, intermediate activity heparin and low activity heparin are 228 units/mg, 142 units/mg and 95 units/mg, respectively. Both the uronic acid content and the quantity of N-SO4 for all three heparin subfractions have been evaluated. The high activity heparin has the lowest uronic acid and N-SO4 content. The successful separation of commercial heparin into three distinct subfractions by means of ion-exchange chromatography suggests that the net charge on these three heparin components will serve as a model system in the elucidation of the structure and activity relationship to the biological function of heparin.

Digestibility and chemical composition of fractions of lucerne during spring and summer

1970 ◽  
Vol 75 (2) ◽  
pp. 213-222 ◽  
Author(s):  
K. R. Christian ◽  
D. B. Jones ◽  
M. Freer
Keyword(s):  
Chemical Composition ◽  
Dry Matter ◽  
Dry Weight ◽  
Chemical Components ◽  
Shoot Height ◽  
Time Of Year ◽  
Highly Correlated ◽  
Leaf Composition

SUMMARYBulk harvests of primary growth were made at intervals during the spring and summer of 2 consecutive years from an ungrazed area of lucerne (Medicago sativa) and the digestibility by sheep was measured. At the same times, shoots of known length representative of the sward were collected, divided into 7·5 cm lengths, and separated into leaf and stem for the determination of ash, nitrogen, cellulose, and in vitro organic matter digestibility.Digestibility and leaf: stem ratio of the bulk harvests decreased and dry matter increased as the season advanced. Leaf composition changed little, but stem increased in lignin and decreased in ash and in vitro digestibility. In vitro digestibility and leaf: stem ratio were highly correlated with sheep digestibility.Chemical composition of shoots of known heights was similar to that of the bulk harvests. Leaf weights/shoot tended to increase linearly with height of shoot, whereas stem increased nearly as the square of the height. Dry matter of leaf and stem were inversely related to leaf:stem ratio. Stem digestibility decreased with leaf:stem ratio and with increase in shoot height.Chemical components of shoot fractions were associated with each other and were largely dependent on the distance from the top of the shoot at which they had been taken, irrespective of shoot height or time of year. Bottom stems had lower ash, nitrogen and digestibility but higher cellulose, dry weight and dry matter than top stems, whose composition approached that of leaves. Leaf fractions showed little consistent trend with position on the shoot.Some of the difficulties in studying changes in plant composition under field conditions are discussed.

scholarly journals Chemical composition of fruits of some important chestnut cultivars

2006 ◽  
Vol 49 (2) ◽  
pp. 183-188 ◽  
Author(s):  
Ümran Ertürk ◽  
Cevriye Mert ◽  
Arif Soylu
Keyword(s):  
Chemical Composition ◽  
Total Protein ◽  
Dry Matter ◽  
Invert Sugar ◽  
Total Sugar ◽  
Chemical Compositions ◽  
Total Carbohydrates ◽  
Total Fat ◽  
Matter Basis

In this study, chemical compositions of the fruits of some important domestic chestnut types and cultivars were investigated. They contained (g/100g dry matter basis) total carbohydrates 75.32 - 86.31, total sugar 10.32 - 22.79, invert sugar 0.08 - 1.25, starch 54.45 - 69.70, sucrose 8.86 - 21.28, ash 1.02 - 3.22, crude cellulose 3.58 - 5.96, total fat 0.49 - 2.01, total protein 4.88 - 10.87. Ca, Mg, Fe, Mn, Cu, Zn, P, Na and K contents were (mg/100g) 43 - 230, 70 - 160, 0.4 - 5.7, 0.7 - 5.5, 0.6 - 3.8, 1.8 - 9.1, 107 - 191, 6 - 41, 761 - 1271, respectively.

scholarly journals Composición química de la morera (Morus alba), para uso en la alimentación animal: densidades y frecuencias de poda.

2006 ◽  
Vol 11 (1) ◽  
pp. 41 ◽  
Author(s):  
Carlos Boschini ◽  
Herbert Dormond-H ◽  
Álvaro Castro-H
Keyword(s):  
Chemical Composition ◽  
Crude Protein ◽  
Dry Matter ◽  
Annual Rainfall ◽  
Chemical Components ◽  
Experimental Station ◽  
Average Annual Rainfall ◽  
Cutting Frequency ◽  
Cellular Wall ◽  
Planting Distance

This experiment was carried out at the Alfredo Volio Mata Dairy Cattle Experimental Station in Costa Rica, at an altitude of 1542 masl, where there is an average annual rainfall of 2050 mm, temperature of 19.5 C and relative humidity of 84%. A mulberry bush plantation was divided into three plots: with a distance of 60, 90 and 120 cm between rows and plants. Each plot was uniformly pruned at two heights: 30 and 60 cm from the ground. During a 336-day period, these subplots were pruned consecutively every 56th, 84th and 112th day. The planting distance and the pruning height produced small variations in the chemical composition of the mulberry leaves and stems. The cutting frequency produced changes in the composition of the leaves and stems. Nevertheless, the differences in dry matter, crude protein, structural carbohydrates and total ashes in the leaves were not higher then 3% in experimental frequencies. In the stems the greatest changes were 9% and they were produced in the content of the dry matter, cellular wall and in some of the structural chemical components, such as cellulose. The cutting number within each frequency significantly influenced the chemical composition of the  leaves and the stems.

scholarly journals The Effect of Ensiling on the Nutritional Composition and Fermentation Characteristics of Brown Seaweeds as a Ruminant Feed Ingredient

Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1019 ◽  
Author(s):  
Mairead Campbell ◽  
Jordi Ortuño ◽  
Lauren Ford ◽  
David R. Davies ◽  
Anastasios Koidis ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Dry Matter ◽  
Antimicrobial Agents ◽  
Animal Feed ◽  
Brown Seaweed ◽  
Nutritional Composition ◽  
Feed Ingredient ◽  
Brown Seaweeds ◽  
Preservation Method ◽  
Ruminant Feed

Ensiling could be an effective method to preserve seaweeds for animal feed applications, however, there is limited scientific knowledge in this area. Seaweeds are a promising ruminant feed ingredient, in part due to the content of phenolic compounds, which are receiving considerable interest as alternative antimicrobial agents in feed. The aim of the study was to compare the effect of ensiling on the nutritional composition and fermentation characteristics of two brown seaweed species, Fucus vesiculosus (FV) and Saccharina latissimi (SL) with or without the use of a Lactobacillus plantarum (LAB) inoculant. The effect of ensiling on the stability of phlorotannin was also investigated using nuclear magnetic resonance (NMR). After harvesting, the seaweeds were wilted for 24 h and subsequently ensiled in laboratory-scaled silos for 90 days. SL silage showed a stronger fermentation pattern (pH < 4), dominated by lactic acid (50–60 g/kg Dry Matter (DM)), and a slightly higher acetic acid content compared to FV silages (p < 0.05). The fermentability of FV was limited (pH > 4.8) with low lactic acid production (<5 g/kg DM). The addition of the LAB inoculant showed no effect on the fermentation process but a modest effect on the chemical composition of both species was observed after the 90-day ensiling period. The results showed no losses in the nutrient content of FV after ensiling, however losses in the Crude Protein (CP, −32%), ash (−36%), Neutral Detergent Fibre (NDF, −77%) and Acid Detergent Fibre (ADF, −58%) content of SL were observed. The ensiling process had a limited effect on the in vitro true dry matter digestibility and phenolic content of either species. Therefore, ensilage may be a suitable preservation method for the use of brown seaweeds as a ruminant feed; however, species-specific differences were observed.

scholarly journals A Novel Antinociceptive Sulphated Polysaccharide of the Brown Marine Alga Spatoglossum Schroederi

2011 ◽  
Vol 6 (6) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Wladimir R. L. Farias ◽  
Paula Cristina W. C. Lima ◽  
Natália Velloso F. C. Rodrigues ◽  
Rômmulo Celly L. Siqueira ◽  
Renata M. F. Amorim ◽  
...  
Keyword(s):  
Marine Alga ◽  
Sugar Content ◽  
Antinociceptive Effect ◽  
Deae Cellulose ◽  
Brown Seaweed ◽  
Exchange Chromatography ◽  
Sulfated Polysaccharides ◽  
Second Phase ◽  
Hot Plate ◽  
Hot Plate Test

Sulfated polysaccharides (SP) of brown algae (Phaeophyta) are composed mainly of α- L-fucose, being classified as fucans, with recognized role in inflammation but not in nociception, which was already described for SP obtained from red algae. Here the SP of the brown marine alga S. schroederi (named Ss-SP) was isolated and assayed for the antinociceptive effect. Ss-SP was isolated by DEAE-cellulose, analyzed by agarose gel electrophoresis and evaluated in nociception models (Formalin, Hot plate, Von Frey) using Swiss mice (20-25g). Anion exchange chromatography provided four major fractions being F1 (Ss-SP) that of highest metachromatic activity and sugar content. Ss-SP inhibited both phases of the formalin test. In the first phase the paw licking (55.2±8.07s) was reduced by 45% (30.5±6.51s) and 40% (32.85±8.66s) at 0.1 and 1 μg/kg, respectively. In the second phase, Ss-SP was also inhibitory about 39%, but only at 1 mg/kg (83.0±15.70s) compared to formalin (136.8±10.27s). This inhibitory effect suggests a mixed mechanism similar to morphine, which was not confirmed in the hot plate test, a model of pain associated with central neurotransmission. However, Ss-SP reduced the animal reaction in response to stimulation withVon Frey filament at the 2nd and 3rd h (20.8±6.86% versus carrageenan: 47.9±5.83%; 33.3±7.71% versus carrageenan: 62.5±9.83%). Accordingly, the paw edema induced by carrageenan (0.08±0.01g) was potently reduced in 45.35% by Ss-SP pre-treatment (0.02±0.003g), corroborating the anti-inflammatory activity demonstrated for brown seaweed polysaccharides. In conclusion our data revealed for the first time the antinociceptive effect of Ss-SP which could be used as a new source of analgesic substances.

scholarly journals Influence of the period of year on the chemical composition and digestibility of pasture and fodder selected by goats in caatinga

2017 ◽  
Vol 18 (3) ◽  
pp. 402-416 ◽  
Author(s):  
Messias José dos Santos SILVA ◽  
Dulciene Karla de Andrade SILVA ◽  
André Luiz Rodrigues MAGALHÃES ◽  
Kedes Paulo PEREIRA ◽  
Érica Carla Lopes da SILVA ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Dry Matter ◽  
Neutral Detergent Fiber ◽  
Semiarid Region ◽  
Mineral Matter ◽  
In Vitro Digestibility ◽  
Randomized Design ◽  
Total Carbohydrates ◽  
Completely Randomized Design

SUMMARY This study aimed to verify the influence of months on chemical composition and in vitro digestibility of pasture and forage selected by goats in the semiarid region of Brazil’s Pernambuco state. Six male goats fistulated in the rumen were used in a completely randomized design. Forage collection was conducted in herbaceous and shrub/tree layers, in order to simulate goat grazing, and materials were separated into leaves and branches. Extrusa and pasture collections were held in November, January and February/March. There was collection month (P <0.05) effect for pasture on dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), with average values of 458.27g/kg NM and 155.46, 521.30 and 390.13g/kg DM, respectively. For the stem (P <0.05) for DM, Organic matter (OM), mineral matter (MM), ethereal stratum (EE), ADF, acid detergent unavailable protein (AIP), total carbohydrates (TCHO) and dry matter in vitro digestibility (DMIVD), with average values of 487.47g/kg NM and 930.80, 69.07, 20.67, 440.30 and 63.78g/kg DM, respectively. About the extrusa, there was collection time effect (P <0.05) on the DM, OM, MM, CP, EE, AIP, TCHO, Non-fibrous carbohydrates (NFC) and DMIVD, with average values of 84.53g/kg DM, 863.03, 136.97, 152.90, 33.60, 14.07, 676.37 and 55.35g/kg DM, respectively. Rainfall changes in the rainy season in the caatinga altered pasture quality, causing changes in the diet selected by goats, mainly in DM, CP, CNF and DMIVD.

Studies of the effects of manuring and season on the yield and chemical composition of the cattle cabbage

1960 ◽  
Vol 55 (2) ◽  
pp. 165-173 ◽  
Author(s):  
D. J. C. Jones
Keyword(s):  
Chemical Composition ◽  
Crude Protein ◽  
Dry Matter ◽  
Weather Conditions ◽  
Growing Season ◽  
Chemical Components ◽  
Dry Matter Yield ◽  
Ether Extract ◽  
Crude Fibre ◽  
Phosphorus And Potassium

1. Ten manurial treatments were applied annually to cattle cabbage grown in a six-course rotation for 7 years. Samples were taken in the early winter each year, and from five harvests taken at fortnightly intervals in the last year.2. The dry-matter yields each year closely followed the weather conditions during the growing season. There was no significant fall in the dry-matter yield during the last season. The treatments supplying phosphorus or phosphorus and potassium substantially increased yield, but the application of nitrogen did not.3. The application of nitrogen, potassium or phosphorus increased the levels of these constituents in the crop in practically all cases. The application of phosphorus produced hearted plants which contained significantly less dry matter than the openleavod plants produced in its absence. The levels of ether extract, crude fibre, ash, silica and magnesium wore not significantly affected by the manorial treatments.4. There was little variation in composition as the season advanced except for significant increases in the crude-fibre levels and decreases in the nitrogen-free extractives levels.5. The distribution of the chemical components was investigated in the outer leaves, heart leaves and stem of the plant. This was compared with that in the marrow-stem kales.6. The chemical composition of the cattle cabbage compares fairly closely with that found for the marrow-stem kales grown under similar conditions. It is, however, lower in dry matter, crude fibre, magnesium and chlorine. It may be considered as a replacement for marrow-stem kale as a source of crude protein and all the major minerals except chlorine and possibly magnesium.

Fucoxanthin Content and Chemical Composition in Brown Seaweed, Nizamuddinia zanardinii (Phaeophyta) Collected from Mirbat, Southern Oman (The Arabian Sea)

2017 ◽  
Vol 19 (3) ◽  
pp. 259-270
Author(s):  
M. Chesalin ◽  
S. Al-Ghassani ◽  
V. I. Ryabushko ◽  
N. I. Bobko ◽  
E. Gureeva ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Arabian Sea ◽  
Brown Seaweed ◽  
Nizamuddinia Zanardinii

Extracellular polysaccharides from suspension cultures of Nicotiana plumbaginifolia

2020 ◽  
Author(s):  
Ian Sims ◽  
A Bacic
Keyword(s):  
Uronic Acid ◽  
Cell Suspension Cultures ◽  
Anion Exchange Chromatography ◽  
Nicotiana Plumbaginifolia ◽  
Suspension Cultures ◽  
Exchange Chromatography ◽  
Arabinogalactan Protein ◽  
Extracellular Polysaccharides ◽  
Selective Precipitation ◽  
The Individual

The soluble polymers secreted by cell-suspension cultures of Nicotiana plumbaginifolia contained 78% carbohydrate, 6% protein and 4% inorganic material. The extracellular polysaccharides were separated into three fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7 and the individual polysaccharides in each fraction were then isolated by selective precipitation and enzymic treatment. Monosaccharide and linkage compositions were determined for each polysaccharide after reduction of uronic acid residues and the degree of esterification of the various uronic acid residues in each polysaccharide was determined concurrently with the linkage types. Six components were identified: an arabinoxyloglucan (comprising 34% of the total polysaccharide) and a galactoglucomannan (15%) in the unbound neutral fraction, a type II arabinogalactan (an arabinogalactan-protein, 11%) and an acidic xylan (3%) in the first bound fraction, and an arabinoglucuronomannan (11%) and a galacturonan (26%) in the second bound fraction. © 1995.

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