Production of antioxidant compounds from tissue culture of Artemisia annua

Prihantini AI, Sukito A, Tachibana S. 2018. Production of antioxidant compound from tissue culture of Artemisia annua. Nusantara Bioscience 10: 251-255. Phenolic compounds have attracted much attention because of their role in some oxidative diseases. Therefore, the study aimed to investigate the production of antioxidant compound from tissue culture of A. annua. The callus were developed on solid MS medium treated with four combination of plant growth regulators: NAA 0.5 mg/L + BA 0.5 mg/L; NAA 0.5 mg/L + kinetin 0.5 mg/L; 2,4-D 0.5 mg/L + BA 0.5 mg/L; and 2,4-D 0.5 mg/L+ kinetin 0.5 mg/L. The callus extracts were evaluated their antioxidant activity and were analyzed the content of phenolic content. Furthermore, detection and quantification of phenolic compound were investigated. Combination of NAA 0.5 mg/L + BA 0.5 mg/L showed the highest DPPH radical scavenging activity and total phenolic content. GCMS analysis also revealed that the combination also showed the highest content of caffeic acid, a phenolic compound known as an antioxidant agent. Furthermore, the callus extract treated with NAA 0.5 mg/L + BA 0.5 mg/L increased both the antioxidant activity and caffeic acid content compared with A. annua leaf extract. In conclusion, plant tissue culture enhanced pharmaceutical properties of A. annua. Moreover, the study suggested that the treated callus extract of A. annua is potential to be further developed for production of antioxidant compound.

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Investigation on Antioxidant Compounds from Marine Algae Extracts Collected from Binuangeun Coast, Banten, Indonesia

SQUALEN Bulletin of Marine and Fisheries Postharvest and Biotechnology ◽

10.15578/squalen.v11i2.243 ◽

2017 ◽

Vol 11 (2) ◽

pp. 59 ◽

Cited By ~ 1

Author(s):

Muhammad Nursid ◽

Endar Marasskuranto ◽

Karren Budi Atmojo ◽

Mulyo Pandu Hartono ◽

Maria Diyah Nur Meinita ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Compound ◽

Total Phenolic Content ◽

Marine Algae ◽

Phenolic Content ◽

Antioxidant Properties ◽

Total Phenolic ◽

Antioxidant Compounds ◽

Antioxidant Power ◽

Antioxidant Compound

Marine algae contains bioactive secondary metabolites that have potential to be developed as antioxidant. The aims of this research were to investigate antioxidant activity and total phenolic compound of marine algae collected from Binuangeun Beach, Banten and to characterize antioxidant compounds from selected algae species. Antioxidant activity was determined by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) assay and Ferric Ion Reducing Antioxidant Power (FRAP) Assay, while total phenolic content was estimated by Folin-Ciocalteau method. Isolation of bioactive antioxidant compound was performed using SiO2 column chromatography and preparative high performance liquid chromatography (HPLC). The antioxidan activity evaluation was conducted to methanol extracts from 20 species (7 Chlorophyta, 9 Phaeophyta and 4 Rhodophyta). Results showed that Padina australis extract was found to be the most active. The major bioactive antioxidant compound was identified as fucoxanthin and a polar compound that was suspected as phenolic compound. The extracts of P. australis presented the highest phenolic content (58.59 mg GAE/g). A significant correlation between antioxidant capacity and total phenolic content was found, indicating that phenolic compounds are the major contributors to the antioxidant properties of P. australis.

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Total Phenolic Content and DPPH Free Radical Scavenging Activity of Young Turmeric Grown in Southern Thailand

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.886.61 ◽

2019 ◽

Vol 886 ◽

pp. 61-69

Author(s):

Suriyan Sukati ◽

Warachate Khobjai

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Total Phenolic ◽

Antioxidant Compounds ◽

Scavenging Activity ◽

Methanolic Extracts ◽

Southern Thailand

Turmeric (Curcuma longa L.), a member of Zingiberacaeae, is used for Thai traditional medicine, flavoring, preservative, and coloring agent. In Southern Thailand, turmeric is a famous ingredient for food recipes. Not only is the ripened rhizome used for cuisine, but also the young rhizomes. Previous studies have indicated that a variety of turmeric products from the ripened rhizome are valuable source of antioxidant compounds. However, there are ambiguous data regarding the level of antioxidant activity of the young rhizome. The aim of this study was to investigate the level of total phenolic content (TPC) and antioxidant activity of the young rhizomes by comparing to the ripened rhizome. TPC and antioxidant activity of aqueous and 80% methanolic extracts from the young turmeric grown in Southern Thailand were estimated using the Folin-Ciocalteu method and DPPH free radical scavenging activity (DPPH-RSA) assay, respectively. Results showed that the concentration of TPC determined in methanolic extract (19.80±0.25 mg GAE/ g extract) from the young rhizome was significantly higher than the corresponding aqueous extract (18.38±0.41 mg GAE/ g extract). The aqueous and methanolic extracts exhibited significant inhibition in DPPH-RSA with the half-maximal inhibitory concentration (IC50) value of 5.88±0.70 mg/mL and 3.00±0.31 mg/mL, respectively. However, in comparison with the ripened rhizome, TPC and DPPH-RSA of the young rhizome were lower. These results indicate that the young rhizome grown in Southern Thailand could be a source of antioxidant compounds, but the ripened rhizome provides better antioxidant properties than the young rhizome.

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Antifungal and Antioxidant Potential of Methanolic Extracts from Acorus calamus L., Chlorella vulgaris Beijerinck, Lemna minuta Kunth and Scenedesmus dimorphus (Turpin) Kützing

Applied Sciences ◽

10.3390/app11114745 ◽

2021 ◽

Vol 11 (11) ◽

pp. 4745

Author(s):

Toncho Dinev ◽

Milena Tzanova ◽

Katya Velichkova ◽

Diyana Dermendzhieva ◽

Georgi Beev

Keyword(s):

Antioxidant Activity ◽

Chlorella Vulgaris ◽

Phenolic Content ◽

Radical Scavenging ◽

Total Phenolic ◽

Acorus Calamus ◽

Total Flavonoid Content ◽

Flavonoid Content ◽

Methanolic Extracts ◽

Scenedesmus Dimorphus

Plant extracts are an important alternative to antibiotics, which are ever more restricted because of their developing microbial resistance and some adverse effects that have been observed following frequent application. The aim of the present study was to determine the antifungal and antioxidant activity of the methanolic extracts of Acorus calamus, Chlorella vulgaris, Lemna minuta and Scenedesmus dimorphus. The antifungal activity of the extracts against strains of Aspergillus flavus, Aspergillus parasiticus, Aspergillus ochraceus, Aspergillus niger, Aspergillus carbonarius, Fusarium graminearum, Fusarium oxysporum, Penicillium chrysogenum and Alternaria alternata was evaluated via the agar well diffusion method. The antioxidant activity of the extracts was measured through the determination of three parameters—total phenolic content, total flavonoid content and radical scavenging potential (determined through UV/Vis analysis). A. calamus extracts had the highest antimicrobial activity against eight fungal strains, followed by the C. vulgaris, L. minuta and S. dimorphus extracts, which were inhibitory against two to three strains. Among the extracts from the species studied, the extract from S. dimorphus showed the highest antioxidant potential, as determined via the DPPH (1,1’-diphenyl-2-picrylhydrazil-radical) method. This correlated to its high total phenolic and flavonoid content. From A. calamus and L. minuta, methanolic extracts were obtained that exhibited similar values of the aforementioned parameters, followed by C. vulgaris extracts, which showed the lowest antioxidant activity. Based on the Pearson correlation coefficients, the impacts of the total phenolic content and the total flavonoid content on radical scavenging capacity are similar, and flavonoids were a significant part of the total phenolic compounds extracted from the plant materials studied.

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New insights of phenolic compounds from optimized fruit extract of Ficus auriculata

Scientific Reports ◽

10.1038/s41598-021-91913-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

M. Shahinuzzaman ◽

Parul Akhtar ◽

N. Amin ◽

Yunus Ahmed ◽

Farah Hannan Anuar ◽

...

Keyword(s):

Antioxidant Activity ◽

Bioactive Compounds ◽

Total Phenolic Content ◽

Phenolic Content ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Fruit Extract ◽

Optimum Extraction

AbstractIn this study, the extraction conditions extracted maximize amounts of phenolic and bioactive compounds from the fruit extract of Ficus auriculata by using optimized response surface methodology. The antioxidant capacity was evaluated through the assay of radical scavenging ability on DPPH and ABTS as well as reducing power assays on total phenolic content (TPC). For the extraction purpose, the ultrasonic assisted extraction technique was employed. A second-order polynomial model satisfactorily fitted to the experimental findings concerning antioxidant activity (R2 = 0.968, P < 0.0001) and total phenolic content (R2 = 0.961, P < 0.0001), indicating a significant correlation between the experimental and expected value. The highest DPPH radical scavenging activity was achieved 85.20 ± 0.96% at the optimum extraction parameters of 52.5% ethanol (v/v), 40.0 °C temperature, and 22 min extraction time. Alternatively, the highest yield of total phenolic content was found 31.65 ± 0.94 mg GAE/g DF at the optimum extraction conditions. From the LC–ESI–MS profiling of the optimized extract, 18 bioactive compounds were tentatively identified, which may regulate the antioxidant activity of fruits of F. auriculata.

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Effects of solvent extraction system on antioxidant activity of Lamium purpureum L.

Hemijska industrija ◽

10.2298/hemind160518047g ◽

2017 ◽

Vol 71 (5) ◽

pp. 361-370

Author(s):

Slavica Grujic ◽

Ana Dzamic ◽

Violeta Mitic ◽

Vesna Stankov-Jovanovic ◽

P.D. Marin ◽

...

Keyword(s):

Antioxidant Activity ◽

Methanol Extract ◽

Phenolic Content ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Radical Scavenger ◽

Scavenging Activity

Antioxidant and free radical scavenging activity of methanol, ethanol, ethyl acetate and chloroform extracts of aerial parts of Lamium purpureum L. was determined by DPPH, ABTS, FRAP and TRP assays. Contents of flavonoids and phenols were also investigated. The total phenolic content in the extracts, determined using Folin?Ciocalteu assay, ranged between 8.57 to 128.00 mg GAE/g d.e. while concentrations of flavonoids in the extracts varied from 24.20 to 39.80 mg QuE/g d.e. The highest phenolic content was found in methanol extract (128.00 mg GAE/g d.e.). The highest content of total flavonoids was identified in the methanol extract (39.80 mg QuE/g d.e.) and the lowest was in the chloroform (24.30 mg QuE/g d.e.). DPPH scavenging of the extracts was determined and obtained IC50 values ranged from 0.12 to 3.12 mg/mL of solution. The values of ABTS radical scavenging activity ranged from 0.35 to 1.80 mg AA/g. The highest ABTS antiradical activity was registered for methanol extract. The FRAP value was found within the range 0.08 to 1.04 ?mol Fe/mg. The best radical scavenger was methanol (1.04 ?mol Fe/mg). In reducing power assay different extracts of L. purpureum showed increasing of activity with increased concentration, and all extracts possessed substantial dose dependent antioxidant activity. The best reducing capacity was obtained with methanol extract of L. purpureum (0.0132 mg AA/mL). The results in this study confirmed that L. purpureum possesses moderate antioxidant properties.

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Antioxidant and Total Phenolic Content of Breadfruit (Artocarpus altilis) Leaves

MATEC Web of Conferences ◽

10.1051/matecconf/201815006007 ◽

2018 ◽

Vol 150 ◽

pp. 06007 ◽

Cited By ~ 1

Author(s):

Lee Yit Leng ◽

Nuramira binti Nadzri ◽

Khor Chu Yee ◽

Norawanis binti Abdul Razak ◽

Abdul Razak Shaari

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Copper Ions ◽

Radical Scavenging ◽

Chemical Components ◽

Total Phenolic ◽

Ftir Analysis ◽

Artocarpus Altilis ◽

Methanolic Extracts

This work aims to determine antioxidant, total phenolic content and Fourier transform infrared spectroscopy (FTIR) analysis of breadfruit leaves which are essential in management of diabetes. The methanolic extracts of breadfruit leaves was used to analyze for total phenolic content and antioxidant. Total phenolic content of the extracts was measured using the Folin–Ciocalteu assay while the antioxidant activity of plant extracts was measured by the 1, 1-diphenyl-2-picryhyradrazyl (DPPH) radical scavenging assay. FTIR analysis was used to determine the chemical components in the leaves. Total phenolic content in fresh breadfruit leaves (144.16 mg/g ± 17.98) was comparable to those of green tea. The results showed the breadfruit leaves extracts exhibited potent antioxidant activity. The presence of OH group also suggests antioxidant capacity of breadfruit leaves to deactivate free radicals as glucose itself could react with hydrogen peroxide in the presence of iron and copper ions to form hydroxyl radical.

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ANTIOXIDANT ACTIVITY AND ANTIPROLIFERATIVE ACTION OF METHANOLIC EXTRACT OF LIQUORICE (GLYCYRRHIZA GLABRA) IN HEPG2 CELL LINE

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i9.11954 ◽

2016 ◽

Vol 8 (9) ◽

pp. 293 ◽

Cited By ~ 3

Author(s):

Dasharath B. Shinde ◽

Santosh S. Koratkar ◽

Neeti Sharma ◽

Ajinkya A. Shitole

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Hepg2 Cells ◽

Phenolic Content ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Glycyrrhiza Glabra ◽

Total Phenolic ◽

Hepg2 Cell Line

Objective: To evaluate the in vitro antioxidant activity of liquorice (Glycyrrhiza glabra) against H2O2 induced oxidative stress in HepG2 cell line.Methods: Antioxidant activity of methanolic extracts of Glycyrrhiza glabra was investigated by measuring total phenolic content using folin-ciocalteu reagent (FCR), free radical scavenging activity by DPPH and ferric reducing antioxidant power (FRAP). The presence of phenolic compounds and flavonoids in the extract was confirmed by Liquid Chromatography-Mass Spectrometry (LC-MS) analysis. Furthermore, the protective effect of methanolic extract of Glycyrrhiza glabra against oxidative stress induced by H2O2 in HepG2 cells was investigated by MTT assay. HepG2 cells were exposed with five different treatments viz. liquorice, H2O2, ascorbic acid, H2O2+liquorice and H2O2+ascorbic acid, to explore the effect of the extract on malondialdehyde (MDA) production, catalase activity, and glutathione reductase levels.Results: The total phenolic content estimated in Glycyrrhiza glabra extract was found to be 241.47 µg per 1000 µg/ml of methanolic extract. It was found that as the concentration of the extract was increased both the free radical scavenging activity and ferric ion reducing power was also found to increase. LC-MS analysis confirmed the presence of eight different phenolic compounds in the methanolic extract which are possibly contributing to the antioxidant activity exhibited by the extract. It was also observed that liquorice treated HepG2 cells showed lower MDA and higher glutathione and catalase levels as compared to only H2O2 treated HepG2 cells where increased MDA production, decreased glutathione reductase and catalase production was observed.Conclusion: Our results thus conclude that, the methanolic extract of Glycyrrhiza glabra can be used as natural supplements in various disease conditions where oxidative stress has been reported.

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Total Phenolic Content and Antioxidant Activity of Salvia spp. Exudates

Natural Product Communications ◽

10.1177/1934578x1200700221 ◽

2012 ◽

Vol 7 (2) ◽

pp. 1934578X1200700 ◽

Cited By ~ 1

Author(s):

Laura Giamperi ◽

Anahi Bucchini ◽

Angela Bisio ◽

Emanuela Giacomelli ◽

Giovanni Romussi ◽

...

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Radical Scavenging ◽

Natural Antioxidants ◽

Total Phenolic ◽

Dpph Radical ◽

Radical Scavenging Capacity ◽

Scavenging Capacity ◽

Dpph Radical Scavenging

The total phenolic content and antioxidant activity of 6 Salvia spp. exudates were measured to find new potential sources of natural antioxidants. Total phenolic content was assessed by a modified Prussian blue method, and the antioxidant activity by two methods: 1,1- diphenyl-2-picryl- hydrazyl (DPPH) radical scavenging capacity assay and lipoxygenase inhibitory assay. The total phenolic content ranged between 1.3 μg/mg DW ( S. fallax) and 74.0 μg/mg DW ( S. cacaliaefolia). In the DPPH test, S. cacaliaefolia was more effective than BHT, while in the inhibition of lipid peroxidation all the extracts presented good antioxidant capacity.

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Total Phenolic Content and Antioxidant Activity of Yacon (Smallanthus Sonchifolius Poepp. and Endl.) Chips: Effect of Cultivar, Pre-Treatment and Drying

Agriculture ◽

10.3390/agriculture8120183 ◽

2018 ◽

Vol 8 (12) ◽

pp. 183 ◽

Cited By ~ 1

Author(s):

Forough Khajehei ◽

Jens Hartung ◽

Simone Graeff-Hönninger

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Total Phenolic ◽

Scavenging Activity ◽

Lime Juice ◽

Smallanthus Sonchifolius ◽

Hot Air ◽

Pre Treatment

Recent studies have associated the consumption of yacon root as a functional plant food with reduced glycemic index and, due to its considerable phenolic acid levels, a protection of cell membranes against free radical damage. This study examined the effect of four different treatments including: (1) storage duration after harvest (one and three weeks after harvest); (2) pre-treatment before drying (untreated, pre-treatment with diluted lime juice); (3) drying method (freeze drying (FD) and convective hot air drying (CHAD)); and (4) cultivar (white and red), on the quality of yacon (Smallanthus sonchifolius Poepp. and Endl.) chips in terms of their total phenolic content (TPC) and antioxidant activity (AA) (ABTS (2,2′-Azino-Bis (3-Ethylbenzothiazoline-6-Sulfonic Acid) Diammonium Salt) radical scavenging activity, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity and ferric reducing antioxidant power (FRAP)). Overall, the chips that were produced using pre-treatment with diluted lime juice and FD had the highest amounts of TPC and AA. Regarding the chips produced by means of CHAD, retention of higher TPC and AA was possible with lime-juice pre-treatment and use of higher hot air temperatures. Moreover, chips produced from the white cultivar had higher TPC and AA than chips produced from the red cultivar.

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IN VITRO ANTIOXIDANT ACTIVITY AND GC-MS ANALYSIS OF THE ETHANOLIC EXTRACTS OF TERMINALIA BELLERICA ROXB (BAHEDA)

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i11.14175 ◽

2016 ◽

Vol 8 (11) ◽

pp. 275

Author(s):

Rasna Gupta ◽

Ram Lakhan Singh ◽

Neeraja Dwivedi

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Total Phenolic ◽

Scavenging Activity ◽

Terminalia Bellerica ◽

Ms Analysis ◽

Phytochemical Constituents

Objective: To investigate the antioxidant activity of ethanolic extract of Terminalia bellerica fruit pulp (TBFP), seed (TBS) and bark (TBB), and identification of phytochemical constituents of said extracts.Methods: The antioxidant potential of T. bellerica (TB) parts was evaluated by free radical scavenging activity (FRSA), superoxide anion radical scavenging activity (SARSA), hydroxyl radical scavenging activity (HRSA) and compared with reference standard quercetin. Lipid peroxidation (LPO), ferric thiocyanate activity (FTC) and reducing power (RP) of the plant extracts were also examined. The phytochemical constituents of said extracts have been quantified by gas chromatography-mass spectrometer (GC-MS) method.Results: Ethanolic extract of TBFP exhibited high phenolic content (254.33) followed by TBS (227.33) and TBB (185) mg/g of GAE. TBFP showed lowest IC50 for FRSA (62 µg/ml), SARSA (39 µg/ml) and HRSA (27 µg/ml) and highest RP (3.39 ascorbic acid equivalents/ml). TBB showed lowest IC50 for LPO (48 µg/ml) whereas TBS showed lowest IC50 for FTC (109 µg/ml). Predominant phyto-compounds present in TB extracts were quinic acid, gallic acid, ethyl galate, 9, 12 octadecadienoic acid and glucopyranose in varying concentrations as analyzed by GC-MS.Conclusion: On the basis of correlation of antioxidant studies with total phenolic content (TPC) and GC-MS analysis of different part of TB, it may be concluded that high concentration of total phenolics and other bioactive phytochemicals of TB extracts make it a potential source of nutraceutical antioxidants.

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