scholarly journals Enterococcus hirae Enteropathy with Ascending Cholangitis and Pancreatitis in a Kitten

2000 ◽  
Vol 37 (3) ◽  
pp. 282-284 ◽  
Author(s):  
J. M. Lapointe ◽  
R. Higgins ◽  
N. Barrette ◽  
S. Milette
Keyword(s):  
Pancreatic Ducts ◽  
Enterococcus Hirae ◽  
Inflammatory Infiltration ◽  
First Report ◽  
Large Numbers ◽  
Enterococcus Spp ◽  
Ascending Cholangitis ◽  
Asymptomatic Adult ◽  
Suppurative Inflammation ◽  
Gram Positive Cocci

A 2-month-old female Persian cat that had been showing episodes of anorexia and diarrhea for the previous 4 weeks was presented in shock and died 2 days later. Numerous Gram-positive cocci were located along the brush border of small intestinal villi, without significant inflammatory infiltration. Similar bacteria were present within hepatic bile ducts and pancreatic ducts and were associated with suppurative inflammation and exfoliation of epithelial cells. Culture of the liver and lung yielded bacteria identified as Enterococcus hirae. Fecal culture from an asymptomatic adult female from the same cattery also yielded large numbers of E. hirae. To our knowledge, this is the first report of E. hirae enteropathy in a cat and the first report of ascending cholangitis and ductal pancreatitis caused by an Enterococcus spp.

Bacterial Osteomyelitis in a 3-Week-Old Broiler Chicken Associated With Enterococcus hirae

2011 ◽  
Vol 48 (6) ◽  
pp. 1134-1137 ◽  
Author(s):  
Ø. Kolbjørnsen ◽  
B. David ◽  
M. Gilhuus
Keyword(s):  
Proximal Femur ◽  
Broiler Chicken ◽  
Bone Lesions ◽  
Liver Necrosis ◽  
Enterococcus Hirae ◽  
Gram Positive ◽  
Growth Depression ◽  
First Report ◽  
Large Numbers ◽  
Enterococcus Spp

Enterococcus hirae infections are reported to cause growth depression, encephalomalacia, endocarditis, and septicemia in chickens. This report describes osteomyelitis in the proximal femur of a 3-week-old broiler chicken that also suffered from valvular endocarditis and liver necrosis. Histologically, clusters of gram-positive coccoid bacteria were found in many organs, including bone lesions. In tissues from 5 of 6 examined chickens from the same flock, E hirae was isolated in large numbers. To the authors' knowledge, this is the first report of spontaneous bacterial osteomyelitis where E hirae was cultured from bone and where coccoid bacteria consistent with Enterococcus spp were simultaneously demonstrated within bone lesions.

scholarly journals Bacteremia Caused by Kocuria kristinae from Egypt: Are There More? A Case Report and Review of the Literature

2016 ◽  
Vol 2016 ◽  
pp. 1-4 ◽  
Author(s):  
Reem M. Hassan ◽  
Dina M. Bassiouny ◽  
Yomna Matar
Keyword(s):  
Case Report ◽  
Review Of The Literature ◽  
Normal Flora ◽  
Gram Positive ◽  
Clinical Specimens ◽  
First Report ◽  
The Family ◽  
Gram Positive Cocci ◽  
Kocuria Kristinae

Kocuria kristinae is opportunistic Gram-positive cocci from the family Micrococcaceae. It is usually considered part of the normal flora that rarely is isolated from clinical specimens. Here, we report a case of Kocuria kristinae bacteremia; to the best of our knowledge, this is the first report from Egypt.

Gastric Dilatation Associated with Gastric Colonization with Sarcina-Like Bacteria in a Cat with Chronic Enteritis

2017 ◽  
Vol 53 (6) ◽  
pp. 321-325
Author(s):  
Joon Young Im ◽  
Set Sokol ◽  
Gerald E. Duhamel
Keyword(s):  
Histological Examination ◽  
Duodenal Mucosa ◽  
Acute Onset ◽  
Gastric Dilatation ◽  
Outflow Obstruction ◽  
First Report ◽  
Gastric Colonization ◽  
Large Numbers ◽  
Abdominal Radiographs ◽  
Duodenal Biopsies

ABSTRACT An 11 yr old spayed female domestic longhair cat was presented for an acute onset of vomiting. Abdominal radiographs and ultrasound revealed severe gastric dilatation (GD) without evidence of gastric outflow obstruction. On esophagogastroduodenoscopy, the duodenal mucosa was mildly erythematous, and a moderate, diffuse, chronic enteritis was found by histological examination of duodenal biopsies. Large numbers of Sarcina-like bacteria without associated inflammation were present in gastric mucosal biopsies. To the authors’ knowledge, this is the first report of GD associated with colonization by Sarcina-like bacteria in a cat. Gastric colonization by Sarcina-like bacteria should be suspected when cats are presented with acute onset of GD and vomiting.

scholarly journals Sialolipoma of the sublingual salivary gland in a dog – first report

2019 ◽  
Vol 56 (1) ◽  
pp. e149060
Author(s):  
Andressa Gianotti Campos ◽  
Geni Patricio ◽  
Patrícia Ferreira de Castro ◽  
Luciane Kanayama ◽  
Alessandra Loureiro Morales dos Santos ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Soft Tissue ◽  
Fibrous Capsule ◽  
Resected Specimen ◽  
Histopathological Analysis ◽  
First Report ◽  
First Case ◽  
Large Numbers ◽  
Soft Tissue Swelling ◽  
The Right

This report describes a 14-year-old Pit Bull dog presenting with a soft tissue swelling of 3-month progression in the right sublingual region. Histopathological analysis of the surgically resected specimen revealed large numbers of mature adipocytes and islets consisting of mucin-containing atrophic acini and dilated ducts surrounded by a thin fibrous capsule. Findings were consistent with sialolipoma of the sublingual salivary gland. To the authors’ knowledge, this is the first case of sialolipoma affecting the sublingual salivary gland in dogs.

scholarly journals First Report of Leaf and Stem Downy Blight of Longan Seedlings Caused by Peronophythora litchii in Taiwan

Plant Disease ◽  
2012 ◽  
Vol 96 (8) ◽  
pp. 1224-1224 ◽  
Author(s):  
P.-J. Ann ◽  
J.-N. Tsai ◽  
H.-R. Yang
Keyword(s):  
Internal Transcribed Spacer ◽  
Leaf Blight ◽  
Natural Host ◽  
Artificial Inoculation ◽  
First Report ◽  
Mature Leaves ◽  
Potential Source ◽  
Large Numbers ◽  
Young Leaves ◽  
Important Disease

Fruit downy blight caused by Peronophythora litchii Chen ex Ko et al. is an important disease of litchi (Litchi chinensis Sonn.) in Taiwan, especially in rainy seasons. Previous records indicate litchi as the only natural host of P. litchii, but this pathogen was found on seedlings of longan (Euphoria longana Lamarck) in 2000. Young seedlings of longan that had emerged in a litchi orchard near Caotun, Nantou County, showed symptoms of droopy leaves and leaf blight. Water-soaked lesions appeared on young leaves, which turned into brown, round or irregular lesions (about 3 to 5 cm long). Diseased leaves withered and collapsed eventually. Dark brown lesions were found on stems of some infected seedlings but none of the infected seedlings were killed. Also, no symptoms were found on mature leaves. The pathogen produced numerous sporangia on sporangiophores on diseased leaves under humid conditions. The disease on young seedlings was observed again in another litchi orchard at Caotun, Nantou County, in 2003. P. litchii was consistently isolated from diseased leaves. Two isolates from colonized longan seedlings, Tari 20250 collected in 2000 and Tari 23301 collected in 2003, were used for further studies. Both isolates produced large numbers of sporangia on long sporangiophores when cultured on 5% V8 agar (5% V8 juice, 0.02% CaCO3, and 1.5% agar). Sporangia produced on the same sporangiophores matured almost simultaneously. Sporangiophores 240 to 1,600 μm (mean 623 μm) branched dichotomously two to eight times. Sporangia were oval or lemon-shaped with semispherical papilla and deciduous with very short pedicels (2 to 5 μm). The dimension was 25 to 55 (35.25) × 15 to 27.5 (21.2) μm for sporangia and 0.5 to 1 (0.55) μm for pedicels. The length/breadth (L/B) ratio of sporangia was 1.3 to 2.14 (1.67). Both isolates produced numerous oospores on 5% V8 agar cultures in darkness. Artificial inoculation tests were done by spraying 5 mL of sporangial suspension (1,000 sporangia/mL) on each longan seedling without wounding. Results showed that both longan isolates of P. litchii were pathogenic on young longan seedlings, causing symptoms similar to those observed on leaves and stems of naturally infected longan seedlings in litchi orchards. Also, both longan isolates of P. litchii caused downy blight on fruits of litchi (L. chinensis var black leaf) by artificial inoculation tests. Moreover, a P. litchi isolate from litchi caused symptoms of leaf blight on young longan seedlings. P. litchii was reisolated from the infected longan tissues. The ribosomal internal transcribed spacer (ITS) sequence confirmed that the longan isolate Tari 20250 (GenBank Accession No. JQ814693) was 100% identical to other P. litchii isolates (GenBank Accession Nos. Gu111613 to Gu111615). To our knowledge, this is the first report of longan as a natural host of P. litchii. The study also suggests that P. litchii on volunteer longan seedlings in litchi orchards may be a potential source of inoculum for fruit downy blight of litchi. References: (1) C. C. Chen. Special Publ. Coll. Agric., Natl. Taiwan Univ. 10:1, 1961. (2) W. H. Ko et al. Mycologia 70:380, 1978.

Influence of Temperature and Gas Permeability of Packaging Film on Development and Composition of Microbial Flora in Vacuum-Packed Bologna-Type Sausage

1983 ◽  
Vol 46 (8) ◽  
pp. 693-698 ◽  
Author(s):  
H.-J. S. NIELSEN
Keyword(s):  
Gas Permeability ◽  
Plate Count ◽  
Gram Negative Bacteria ◽  
Packaging Film ◽  
Influence Of Temperature ◽  
Large Numbers ◽  
Brochothrix Thermosphacta ◽  
Aerobic Plate Count ◽  
Storage Temperatures ◽  
Gram Positive Cocci

Studies were done on the influence of gas permeability of packaging film on microflora of vacuum-packed Bologna-type sausage at three storage temperatures, i.e., 2, 5 and 10°C. Greatest development of total aerobic plate count and Brochothrix thermosphacta was observed in film having the highest permeability. The packaging film influenced the maximum counts and growth rates of organisms. Growth of both gram-positive cocci and yeast were stimulated in packages having the greatest permeabilities, whereas the opposite occurred for the lactic acid bacteria. Large numbers of gram-negative bacteria were found in all series. These consisted of Enterobacteriaceae, Moraxella/Moraxella-like bacteria and atypical Vibrio. Only Enterobacteriaceae were highly influenced by the nature of the packaging film. Organoleptic analyses showed a clear association among odor, the development of microflora and the gas permeability of packaging film.

scholarly journals In vitro resistance to daptomycin of staphylococcus aureus and enterococcus spp. strains

2014 ◽  
Vol 20 (4) ◽  
pp. 195-199
Author(s):  
Irina Stan ◽  
Mihaela Botnarciuc ◽  
Liliana Tuţă ◽  
Emma Gheorghe
Keyword(s):  
Staphylococcus Aureus ◽  
Therapeutic Option ◽  
Diffusion Method ◽  
Gram Positive ◽  
Dna And Rna ◽  
Bacterial Cell Death ◽  
Enterococcus Spp ◽  
Dna And Rna Synthesis ◽  
Gram Positive Cocci

Abstract Daptomycin is the first antibiotic in a new class of cyclic lipopeptides, active in vitro against Gram - positive cocci. The mechanism of action is a rapid depolarization of the bacterial membrane potential, loss of cytoplasmic contents, mainly K+ ions and inhibition of protein, DNA and RNA synthesis, followed by the bacterial cell death. There were investigated 112 strains of Gram-positive cocci recommended for daptomycin susceptibility testing. Since the main clinical indications of daptomycin are the severe skin and soft tissue infections caused by methicillin-resistant Staphylococcus aureus, vancomicin-resistant enterococci and penicillinresistant Streptococcus spp, we tested daptomycin activity only in these species. The susceptibility tests was performed by Kirby- Bauer disc-diffusion method on Muller-Hinton agar supplemented with Ca 50 mg / ml. (CAMH - Ca 2 + ) as recommended by CLSI. During the period in which we conducted the study we isolated 112 strains of Gram-positive cocci, which we tested sensitivity to daptomycin. Resistance to Daptomycin of Staphylococcus aureus (4.11%) was lower than resistance to vancomycin (6.76%), but higher than the resistance to teicoplanin (2.71%) and linezolid (1.38%). In our study we did not identify daptomycin resistant strains of Enterococcus spp. In severe infections that require more aggressive treatment using active antibiotics on MRSA, daptomycin is a valid therapeutic option, but a susceptibility test is required to ensure effective indication. There were no daptomycin resistant Enterococcus spp. strains

scholarly journals First Report of Tomato infectious chlorosis virus in Tomato in Indonesia

Plant Disease ◽  
2003 ◽  
Vol 87 (7) ◽  
pp. 872-872 ◽  
Author(s):  
J. Th. J. Verhoeven ◽  
T. M. Willemen ◽  
J. W. Roenhorst ◽  
R. A. A. van der Vlugt
Keyword(s):  
The United States ◽  
Rt Pcr ◽  
First Report ◽  
Tomato Seedlings ◽  
Large Numbers ◽  
Lateral Shoots ◽  
Young Leaves ◽  
Polymerase Chain ◽  
Tomato Chlorosis Virus ◽  
Tomato Infectious Chlorosis Virus

In 2002, a breeding company submitted several samples of tomato (Lycopersicon esculentum) for diagnosis. Samples originated in Indonesia and were taken from protected and nonprotected crops. Plants exhibited severe chlorosis on fully expanded leaves, while young leaves were symptomless. Symptoms resembled those of the criniviruses Tomato chlorosis virus (ToCV) and Tomato infectious chlorosis virus (TICV). Moreover, large numbers of whiteflies, potential vectors of these viruses, had been observed at the plots with symptomatic plants. A reverse transcription-polymerase chain reaction (RT-PCR) with specific primers for TICV (1) yielded amplicons of the expected size of approximately 500 bp for all samples. One of the amplicons was sequenced (Genbank Accession No. AY221097) and revealed more than 98.9% identity to six isolates of TICV in NCBI Genbank. cDNA synthesis using the universal crinivirus primer HSP_M2-DW (5′ -TCRAARGTWCCKCCNCCRAA-3′) followed by PCR with a ToCV specific primerset (ToCV-UP 5′-TCATTAAAACTCAATGGGACCGAG-3′ and ToCV-DW 5′-GCGACGT AAATTGAAACCC-3′) was negative in all cases. Grafting of symptomatic shoots onto healthy tomato seedlings of cv. Money-maker showed transmission of the virus, as chlorosis appeared on fully expanded leaves of lateral shoots after 6 weeks. The presence of TICV in the graft-inoculated plants was confirmed by RT-PCR. Furthermore, mechanical inoculation to a range of herbaceous test plants did not evoke any virus symptoms, indicating the absence of mechanically transmissible viruses. Although other nonmechanically transmissible viruses cannot be fully excluded, the results together with the symptoms observed, indicate that TICV is the cause of the disease. TICV has been reported from Greece, Italy, Japan, Spain, and the United States, but to our knowledge, this is the first report of TICV in Indonesia. Reference: (1) A. M. Vaira et al. Phytoparasitica 30:290, 2002.

scholarly journals Thyronectria balsamea on Abies fraseri in Pennsylvania and North Carolina

Plant Disease ◽  
1997 ◽  
Vol 81 (7) ◽  
pp. 830-830 ◽  
Author(s):  
N. G. Wenner ◽  
W. Merrill ◽  
J. T. Moody
Keyword(s):  
New York ◽  
Tree Plantations ◽  
State University ◽  
Eastern United States ◽  
Pennsylvania State University ◽  
Arnold Arbor ◽  
Abies Fraseri ◽  
First Report ◽  
Large Numbers ◽  
Voucher Specimens

In August 1996, several 4- to 6-m-tall Abies fraseri (Pursh) Poir. in Adams County, PA, were found bearing numerous dead branches and/or dead tops. The trees had been severely stressed by being ball-and-burlapped and replanted in 1993. Distinct cankers occurred between the living and dead portions of stems and branches. Associated with these cankers were abundant, reddish-orange, erumpent stroma, each bearing three to 10 similarly colored cupulate ascomata. The latter contained asci bearing two to four large, muriform ascospores that, as they matured, formed large numbers of small ascoconidia, indicating the pathogen was Thyronectria balsamea (Cooke & Peck) Seeler (= Nectria balsamea Cooke & Peck). In September 1996, cankered dead stems and branches from affected A. frasrei Christmas tree plantations in Avery County, NC, were found bearing the same pathogen. This fungus is known on A. bal-samea (L.) Mill. from northern Minnesota east through Canada to northern New York and Newfoundland (2). Funk (1) reported it from A. lasio-carpa (Hook.) Nutt. in (presumably) British Columbia, but gave no details. This is the first report of it in the eastern United States south of northern New York, a considerable extension of its known range, and the first report of it from A. fraseri. Voucher specimens are in PACMA (Pennsylvania State University Mycologica Herbarium, Mont Alto Campus). References: (1) A. Funk. Can. For. Serv. BC-X-222:142, 1981. (2) E. V. Seeler, Jr. J. Arnold Arbor. 21:442, 1940.

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