Genetic Variants in microRNA Target Sites of 37 Selected Cancer-Related Genes and the Risk of Cervical Cancer

Influenza A viruses display a broad cellular tropism within the respiratory tracts of mammalian hosts. Uncovering the relationship between tropism and virus immunity, pathogenesis, and transmission will be critical for the development of therapeutic interventions. Here we discuss recent developments of several recombinant strains of influenza A virus. These viruses have inserted reporters to track tropism, microRNA target sites to restrict tropism, or barcodes to assess transmission dynamics, expanding our understanding of pathogen-host interactions.

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Muscle-specific microRNA miR-206 promotes muscle differentiation

The Journal of Cell Biology ◽

10.1083/jcb.200603008 ◽

2006 ◽

Vol 174 (5) ◽

pp. 677-687 ◽

Cited By ~ 523

Author(s):

Hak Kyun Kim ◽

Yong Sun Lee ◽

Umasundari Sivaprasad ◽

Ankit Malhotra ◽

Anindya Dutta

Keyword(s):

Antisense Oligonucleotide ◽

Small Interfering Rna ◽

Degradation Process ◽

Microrna Target ◽

C2c12 Myoblasts ◽

Target Sites ◽

The Many ◽

Interfering Rna ◽

In Vitro Transfection

Three muscle-specific microRNAs, miR-206, -1, and -133, are induced during differentiation of C2C12 myoblasts in vitro. Transfection of miR-206 promotes differentiation despite the presence of serum, whereas inhibition of the microRNA by antisense oligonucleotide inhibits cell cycle withdrawal and differentiation, which are normally induced by serum deprivation. Among the many mRNAs that are down-regulated by miR-206, the p180 subunit of DNA polymerase α and three other genes are shown to be direct targets. Down-regulation of the polymerase inhibits DNA synthesis, an important component of the differentiation program. The direct targets are decreased by mRNA cleavage that is dependent on predicted microRNA target sites. Unlike small interfering RNA–directed cleavage, however, the 5′ ends of the cleavage fragments are distributed and not confined to the target sites, suggesting involvement of exonucleases in the degradation process. In addition, inhibitors of myogenic transcription factors, Id1-3 and MyoR, are decreased upon miR-206 introduction, suggesting the presence of additional mechanisms by which microRNAs enforce the differentiation program.

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The impact of genetic variants inIL1R2on cervical cancer risk among Uygur females from China: A case-control study

Molecular Genetics & Genomic Medicine ◽

10.1002/mgg3.516 ◽

2018 ◽

Vol 7 (1) ◽

pp. e00516 ◽

Cited By ~ 4

Author(s):

Fanglin Niu ◽

Tianchang Wang ◽

Jing Li ◽

Mengdan Yan ◽

Dianzhen Li ◽

...

Keyword(s):

Cervical Cancer ◽

Cancer Risk ◽

Genetic Variants ◽

Case Control Study ◽

Case Control ◽

Cervical Cancer Risk ◽

The Impact ◽

Control Study

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Identification of Genetic Variants of Human Papillomavirus in a Group of Mexican HIV/AIDS Patients and Their Possible Association with Cervical Cancer

Polish Journal of Microbiology ◽

10.33073/pjm-2021-047 ◽

2021 ◽

Vol 70 (4) ◽

pp. 501-509

Author(s):

FELIPE ORTIZ-GUTIÉRREZ ◽

LILIA SÁNCHEZ-MINUTTI ◽

JOSÉ F. MARTÍNEZ-HERRERA ◽

INDIANA D. TORRES-ESCOBAR ◽

ELIAS B. PEZZAT-SAID ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Genetic Variants ◽

Direct Relationship ◽

Low Risk ◽

Hiv Positive ◽

Hiv Positive Women ◽

Immunodeficiency Virus ◽

Regional Population ◽

Polymerase Chain

Infections caused by the human immunodeficiency virus (HIV) and human papillomavirus (HPV) cause thousands of deaths worldwide each year. So far, there has been no consensus on whether there is a direct relationship between the incidence of neoplasms and the immunosuppression caused by HIV that could help understand if coinfection increases the likelihood of cervical cancer. The objective of the study was to identify the presence of genetic variants of HPV in a group of HIV-positive women and their possible association with cervical cancer. Cervical samples were taken from HIV-positive patients for cytological analysis to identify the HPV genotype by polymerase chain reaction (PCR) and sequencing. The most preva¬lent L1 capsid protein mutations in the HPV genotype were ana¬lyzed in silico. Various types of HPV were identified, both high-risk (HR) and low-risk (LR). The most prevalent genotype was HPV51. Analysis of the L1 gene sequences of HPV51 isolates showed nucleo¬tide variations. Of the samples analyzed in Puebla, Mexico, HPV51 had the highest incidence (17.5%, 7/40). Different mutations, which could be used as population markers, were detected in this area, and they have not been reported in the L1 databases for HPV51 in Mexico. Genotypes 6, 14, 86, 87, 89, and 91, not detected or reported in samples from patients with HPV in Mexico, were also identified. Data from the population analyzed suggest no direct relationship between HIV immunosuppression and cervical cancer, regardless of the high- or low-risk HPV genotype. Furthermore, it is possible to develop regional population markers for the detection of HPV based on the mutations that occur in the sequence of nucleotides analyzed.

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Bioinformatic and Genetic Association Analysis of MicroRNA Target Sites in One-Carbon Metabolism Genes

PLoS ONE ◽

10.1371/journal.pone.0021851 ◽

2011 ◽

Vol 6 (7) ◽

pp. e21851 ◽

Cited By ~ 52

Author(s):

Nicole Stone ◽

Faith Pangilinan ◽

Anne M. Molloy ◽

Barry Shane ◽

John M. Scott ◽

...

Keyword(s):

Genetic Association ◽

Association Analysis ◽

Carbon Metabolism ◽

Microrna Target ◽

Genetic Association Analysis ◽

Target Sites ◽

One Carbon Metabolism

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PopTargs: a database for studying population evolutionary genetics of human microRNA target sites

Database ◽

10.1093/database/baz102 ◽

2019 ◽

Vol 2019 ◽

Cited By ~ 2

Author(s):

Andrea Hatlen ◽

Mohab Helmy ◽

Antonio Marco

Keyword(s):

Evolutionary Genetics ◽

Human Populations ◽

Microrna Target ◽

Multiple Sources ◽

Web Based ◽

Regulatory Motifs ◽

Multiple Datasets ◽

Custom Made ◽

Target Sites ◽

Selective Forces

Abstract There is an increasing interest in the study of polymorphic variants at gene regulatory motifs, including microRNA target sites. Understanding the effects of selective forces at specific microRNA target sites, together with other factors like expression levels or evolutionary conservation, requires the joint study of multiple datasets. We have compiled information from multiple sources and compared it with predicted microRNA target sites to build a comprehensive database for the study of microRNA targets in human populations. PopTargs is a web-based tool that allows the easy extraction of multiple datasets and the joint analyses of them, including allele frequencies, ancestral status, population differentiation statistics and site conservation. The user can also compare the allele frequency spectrum between two groups of target sites and conveniently produce plots. The database can be easily expanded as new data becomes available and the raw database as well as code for creating new custom-made databases is available for downloading. We also describe a few illustrative examples.

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