scholarly journals Human Intestinal Cells Modulate Conjugational Transfer of Multidrug Resistance Plasmids between Clinical Escherichia coli Isolates

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e100739 ◽  
Author(s):  
Ana Manuel Dantas Machado ◽  
Morten O. A. Sommer
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Intestinal Cells ◽  
Conjugational Transfer ◽  
Resistance Plasmids ◽  
Human Intestinal Cells

scholarly journals Human milk glycosaminoglycans inhibit in vitro the adhesion of Escherichia coli and Salmonella fyris to human intestinal cells

2015 ◽  
Vol 79 (4) ◽  
pp. 603-607 ◽  
Author(s):  
Giovanni V. Coppa ◽  
Bruna Facinelli ◽  
Gloria Magi ◽  
Emanuela Marini ◽  
Lucia Zampini ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Milk ◽  
Intestinal Cells ◽  
Human Intestinal Cells

Glycopeptide derived from soybean β-conglycinin inhibits the adhesion of Escherichia coli and Salmonella to human intestinal cells

2008 ◽  
Vol 41 (6) ◽  
pp. 594-599 ◽  
Author(s):  
Baichong Yang ◽  
Xuemei Zhang ◽  
Xiaolan Bao ◽  
Ying Lv ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Intestinal Cells ◽  
Human Intestinal Cells

scholarly journals Human Diffusely Adhering Escherichia coli Expressing Afa/Dr Adhesins That Use Human CD55 (Decay-Accelerating Factor) as a Receptor Does Not Bind the Rodent and Pig Analogues of CD55

2004 ◽  
Vol 72 (8) ◽  
pp. 4859-4863 ◽  
Author(s):  
Sylvie Hudault ◽  
O. Brad Spiller ◽  
B. Paul Morgan ◽  
Alain L. Servin
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Cho Cells ◽  
Chinese Hamster ◽  
Intestinal Cells ◽  
Gastrointestinal Infections ◽  
Decay Accelerating Factor ◽  
E Coli ◽  
Empty Vector ◽  
Human Intestinal Cells

ABSTRACT Afa/Dr diffusely adhering Escherichia coli (DAEC) bacteria that are responsible for recurrent urinary tract and gastrointestinal infections recognized as a receptor the glycosylphosphatidylinositol (GPI)-anchored protein decay-accelerating factor (DAF; CD55) at the brush border of cultured human intestinal cells. Results show that Afa/Dr DAEC C1845 bacteria were poorly associated with the mucosa of the gastrointestinal tract of infected mice. We conducted experiments with Chinese hamster ovary (CHO) cells stably transfected with mouse (GPI or transmembrane forms), pig, or human CD55 or mouse Crry cDNAs or transfected with empty vector pDR2EF1α. Recombinant E. coli AAEC185 bacteria expressing Dr or F1845 adhesins bound strongly to CHO cells expressing human CD55 but not to the CHO cells expressing mouse (transmembrane and GPI anchored), rat, or pig CD55 or mouse Crry. Positive clustering of CD55 around Dr-positive bacteria was observed in human CD55-expressing CHO cells but not around the rarely adhering Dr-positive bacteria randomly distributed at the cell surface of CHO cells expressing mouse, rat, or pig CD55.

scholarly journals Host CDK-1 and formin mediate microvillar effacement induced by enterohemorrhagic Escherichia coli

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Cheng-Rung Huang ◽  
Cheng-Ju Kuo ◽  
Chih-Wen Huang ◽  
Yu-Ting Chen ◽  
Bang-Yu Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterohemorrhagic Escherichia Coli ◽  
Intestinal Cell ◽  
Intestinal Cells ◽  
Cyclin Dependent Kinase ◽  
C Elegans ◽  
Mitotic Cyclin ◽  
Human Intestinal Cells

AbstractEnterohemorrhagic Escherichia coli (EHEC) induces changes to the intestinal cell cytoskeleton and formation of attaching and effacing lesions, characterized by the effacement of microvilli and then formation of actin pedestals to which the bacteria are tightly attached. Here, we use a Caenorhabditis elegans model of EHEC infection to show that microvillar effacement is mediated by a signalling pathway including mitotic cyclin-dependent kinase 1 (CDK1) and diaphanous-related formin 1 (CYK1). Similar observations are also made using EHEC-infected human intestinal cells in vitro. Our results support the use of C. elegans as a host model for studying attaching and effacing lesions in vivo, and reveal that the CDK1-formin signal axis is necessary for EHEC-induced microvillar effacement.

scholarly journals A Clinical Extensively-Drug Resistant (XDR) Escherichia coli and Role of Its β-Lactamase Genes

2020 ◽  
Vol 11 ◽  
Author(s):  
Mingyu Wang ◽  
Wenjia Wang ◽  
Yu Niu ◽  
Ting Liu ◽  
Ling Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
High Resistance ◽  
Multidrug Resistant ◽  
Drug Resistant ◽  
Coding Region ◽  
E Coli ◽  
Extensively Drug Resistant ◽  
Resistance Plasmids ◽  
And Function

An extensively-drug resistant (XDR) Escherichia coli W60 was isolated from the urine sample of a patient. The genetic basis for its XDR phenotype was investigated, particularly the basis for its resistance toward β-lactam/BLI (β-Lactamase Inhibitor) combinations. Following determination of the XDR phenotype, third generation genomic sequencing was performed to identify genetic structures in E. coli W60. Further cloning analysis was performed to identify determinants of β-lactam/BLI combination resistance. It was found that E. coli W60 is resistant to nearly all of the tested antibiotics including all commonly used β-lactam/BLI combinations. Analysis of the genomic structures in E. coli W60 showed two novel transferable plasmids are responsible for the resistance phenotypes. Further genetic analysis showed blaNDM–5 leads to high resistance to β-lactam/BLI combinations, which was enhanced by co-expressing bleMBL. pECW602 harbors a truncated blaTEM that is not functional due to the loss of the N-terminal signal peptide coding region. Research performed in this work leads to several significant conclusions: the XDR phenotype of E. coli W60 can be attributed to the presence of transferable multidrug resistance plasmids; NDM-5 confers high resistance to β-lactam/BLI combinations; co-expression of bleMBL enhances resistance caused by NDM-5; the signal peptides of TEM type β-lactamases are essential for their secretion and function. Findings of this work show the danger of transferable multidrug resistance plasmids and metallo-β-lactamases, both of which should be given more attention in the analysis and treatment of multidrug resistant pathogens.

scholarly journals Attachment of enterotoxigenic Escherichia coli to human intestinal cells.

1983 ◽  
Vol 39 (3) ◽  
pp. 1102-1106 ◽  
Author(s):  
C F Deneke ◽  
K McGowan ◽  
G M Thorne ◽  
S L Gorbach
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Intestinal Cells ◽  
Human Intestinal Cells

scholarly journals Plasmid Sequences of Four Large Plasmids Carrying Antimicrobial Resistance Genes in Escherichia coli Strains Isolated from Beef Cattle in Japan

2020 ◽  
Vol 9 (20) ◽  
Author(s):  
Shiori Yamamoto ◽  
Wataru Kitagawa ◽  
Motoki Nakano ◽  
Hiroshi Asakura ◽  
Eriko Iwabuchi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Antimicrobial Resistance ◽  
Beef Cattle ◽  
Resistance Genes ◽  
Content Type ◽  
E Coli ◽  
Antimicrobial Resistance Genes ◽  
Resistance Plasmids ◽  
Large Plasmids

Escherichia coli is a common reservoir for antimicrobial resistance genes that can be easily transformed to possess multidrug resistance through plasmid transfer. To understand multidrug resistance plasmids, we report the plasmid sequences of four large plasmids carrying a number of genes related to antimicrobial resistance that were found in E. coli strains isolated from beef cattle.

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