scholarly journals Acridine orange for diagnosis of malaria – Our experience

2016 ◽  
Vol 6 (1) ◽  
pp. 49-51
Author(s):  
Neeta Jangale ◽  
Ashwini Waghmare
Keyword(s):  
Acridine Orange ◽  
High Performance ◽  
Fluorescent Microscopy ◽  
Peripheral Blood Smear ◽  
Laboratory Diagnosis ◽  
Buffy Coat ◽  
Acridine Orange Staining ◽  
Qualified Personnel ◽  
Blood Smear Examination ◽  
Set Up

Light microscopy of Romanowsky stained peripheral blood smear examination is the age old and commonest method for laboratory diagnosis of malaria. However it is labor intensive, time consuming and requires qualified personnel. So fluorescent microscopy variation that is Quantitative Buffy Coat method( QBC) came into vogue. But QBC imposes cost limitation despite its high performance. Therefore we decided to evaluate fluorescent method using acridine orange in our set up. In this study we have compared Acridine Orange staining with Leishman’s staining as gold standard. The sensitivity and specificity of acridine orange was 99.28% and 97.19% respectively, while positive predictive value (PPV) and negative predicitive value was 89.93% and 99.82% respectively.South East Asia Journal of Public Health Vol.6(1) 2016: 49-51

STUDIES ON THE BIOLOGICAL PROPERTIES AND CLASSIFICATION OF SV4 VIRUS

1965 ◽  
Vol 11 (2) ◽  
pp. 325-335 ◽  
Author(s):  
S. A. Sattar ◽  
K. R. Rozee
Keyword(s):  
Electron Microscope ◽  
Rhesus Monkey ◽  
Red Blood Cells ◽  
Acridine Orange ◽  
Blood Cells ◽  
Magnesium Chloride ◽  
Fluorescent Microscopy ◽  
Biological Properties ◽  
Acridine Orange Staining

Cytopathic changes in LLC-MK2 cells infected with SV4 virus, observed with the electron microscope and using acridine orange staining and fluorescent microscopy, have been shown to be similar to that caused by picornaviruses and members of the Columbia-SK virus group. The virus was found to be stabilized against heat in the presence of molar magnesium chloride, and to be stable at pH 3.5. The virus was non-pathogenic for suckling mice, failed to agglutinate sheep and human "O" red blood cells, but agglutinated rhesus monkey erythrocytes at 4 °C. On the basis of these properties and those already known, it was suggested that SV4 virus be placed in the group Enteroviruses of lower animals.

Stress and in vitro lymphocyte stimulation by phytohemagglutinin in Rocky Mountain bighorn sheep

1973 ◽  
Vol 51 (5) ◽  
pp. 479-482 ◽  
Author(s):  
R. J. Hudson
Keyword(s):  
Acridine Orange ◽  
Fluorescent Microscopy ◽  
Rocky Mountain ◽  
Bighorn Sheep ◽  
Lymphocyte Stimulation ◽  
Serum Samples ◽  
Acridine Orange Staining ◽  
Cellular Changes ◽  
In Captivity

The blastogenic effect of phytohemagglutinin (PHA) on peripheral lymphocytes from six Rocky Mountain bighorn sheep was examined for 3 months following their establishment in captivity. Lymphocytes survived well in culture for 6 days and responded to PHA with morphologic transformation to blastoid cells which were readily identifiable by fluorescent microscopy following acridine orange staining. Transformation of lymphocytes from animals at the time of initial establishment was low but as the animals adjusted to captivity, transformation increased markedly. These observations appeared to reflect predominantly cellular changes since serum samples collected throughout the study did not differ in their ability to support transformation.

scholarly journals Optimization of Dynamic Task Location within a Manipulator’s Workspace for the Utilization of the Minimum Required Joint Torques

Electronics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 288
Author(s):  
Adam Wolniakowski ◽  
Charalampos Valsamos ◽  
Kanstantsin Miatliuk ◽  
Vassilis Moulianitis ◽  
Nikos Aspragathos
Keyword(s):  
High Performance ◽  
Human Robot Interaction ◽  
Optimal Position ◽  
End Effector ◽  
Joint Torques ◽  
Dynamic Task ◽  
Arbitrary Position ◽  
Simulation Results ◽  
Set Up ◽  
Task Placement

The determination of the optimal position of a robotic task within a manipulator’s workspace is crucial for the manipulator to achieve high performance regarding selected aspects of its operation. In this paper, a method for determining the optimal task placement for a serial manipulator is presented, so that the required joint torques are minimized. The task considered comprises the exercise of a given force in a given direction along a 3D path followed by the end effector. Given that many such tasks are usually conducted by human workers and as such the utilized trajectories are quite complex to model, a Human Robot Interaction (HRI) approach was chosen to define the task, where the robot is taught the task trajectory by a human operator. Furthermore, the presented method considers the singular free paths of the manipulator’s end-effector motion in the configuration space. Simulation results are utilized to set up a physical execution of the task in the optimal derived position within a UR-3 manipulator’s workspace. For reference the task is also placed at an arbitrary “bad” location in order to validate the simulation results. Experimental results verify that the positioning of the task at the optimal location derived by the presented method allows for the task execution with minimum joint torques as opposed to the arbitrary position.

scholarly journals CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS

1962 ◽  
Vol 15 (3) ◽  
pp. 535-540 ◽  
Author(s):  
M. Rabinovitch ◽  
W. Plaut
Keyword(s):  
Nucleic Acid ◽  
Dna Synthesis ◽  
Acridine Orange ◽  
Particle Number ◽  
Amoeba Proteus ◽  
Acridine Orange Staining ◽  
Particle Population ◽  
Cell Age ◽  
Cytoplasmic Dna ◽  
Number Of Particles

Nucleic acid-containing particles in the cytoplasm of Amoeba proteus (cf. reference 1) were counted after acridine orange staining. The number of particles per ameba was found to be correlated with cell age and size. Fresh daughters had a mean particle number of 5400, whereas predivision amebae contained around 11,000 particles. Amebae from two other strains contained similar particles. The particles were found to be clustered in fasted cells and redispersed after feeding. A marked increase in the particle population was noted in anucleate fragments. These results, together with those previously presented, suggest that the particles multiply intracellularly. Their nature and their relationship to previous work on nucleic acid labeling in Amoeba are discussed.

Differential-Geometric Methods in Multibody Dynamics and Control

2005 ◽  
Author(s):  
P. Maißer
Keyword(s):  
Configuration Space ◽  
High Performance ◽  
Multibody System ◽  
Equations Of Motion ◽  
Geometric Approach ◽  
Motion Equations ◽  
Constrained Mechanical Systems ◽  
Dynamics And Control ◽  
Set Up ◽  
Lagrangian Motion

This paper presents a differential-geometric approach to the multibody system dynamics regarded as a point dynamics in a n-dimensional configuration space Rn. This configuration space becomes a Riemannian space Vn the metric of which is defined by the kinetic energy of the multibody system (MBS). Hence, all concepts and statements of the Riemannian geometry can be used to study the dynamics of MBS. One of the key points is to set up the non-linear Lagrangian motion equations of tree-like MBS as well as of constrained mechanical systems, the perturbed equations of motion, and the motion equations of hybrid MBS in a derivative-free manner. Based on this approach transformation properties can be investigated for application in real-time simulation, control theory, Hamilton mechanics, the construction of first integrals, stability etc. Finally, a general Lyapunov-stable force control law for underactuated systems is given that demonstrates the power of the approach in high-performance sports applications.

The fluorescent staining of a mycobacteriophage (C2) nucleic acid

1971 ◽  
Vol 17 (2) ◽  
pp. 171-174 ◽  
Author(s):  
Jose Menezes
Keyword(s):  
Nucleic Acid ◽  
Acridine Orange ◽  
Staining Technique ◽  
Fluorescent Staining ◽  
Green Fluorescence ◽  
Acridine Orange Staining ◽  
Fluorescence Characteristic ◽  
Phage Dna ◽  
Acid Dnase

By using the acridine orange staining technique a green fluorescence, characteristic of double-stranded nucleic acid, can be observed with purified preparations of mycobacteriophage C2 and its extracted nucleic acid. DNAse-treated samples do not show this fluorescence, which leads to the conclusion that this fluorescence is associated with phage DNA. Examination of preparations of phage grown in the presence of acridine orange supported these results.

LPC Blade and Non-Axisymmetric Hub Profiling Optimization Using Multi-Fidelity Non-Intrusive POD Surrogates

2017 ◽  
Author(s):  
Tariq Benamara ◽  
Piotr Breitkopf ◽  
Ingrid Lepot ◽  
Caroline Sainvitu
Keyword(s):  
High Performance ◽  
High Efficiency ◽  
High Fidelity ◽  
Present Contribution ◽  
Compressor Blades ◽  
High Fidelity Simulations ◽  
Optimization Methodology ◽  
Set Up ◽  
Automated Optimization ◽  
Proper Orthogonal

The present contribution proposes a Reduced Order Model based multi-fidelity optimization methodology for the design of highly loaded blades in low pressure compressors. Environmental, as well as, economical limitations applied to engine manufacturers make the design of modern turbofans an extremely complex task. A smart compromise has to be found to guarantee both a high efficiency and a high average stage loading imposed for mass reduction constraints, while satisfying stability requirements. The design of compressor blades, usually involves at the same time a dedicated parametrization set-up in highdimensional space and high-fidelity simulations capturing, at least, efficiency and stability as most impacting phenomena. Despite recent advances in the high-performance computing area, introducing high-fidelity simulations into automated optimization, or even surrogate assisted optimization, loops still stands as a endeavor for engineers. In this framework, the proposed methodology is based on multi-fidelity surrogate models capable of representing the physics at hand in reduced spaces inferred from both precise, albeit costly, high-fidelity simulations and abundant, yet less accurate lower-fidelity data. Finally, we investigate the coupling of the proposed hierarchised multi-fidelity non-intrusive Proper Orthogonal Decomposition based surrogates with an evolutionary algorithm to reduce the number of high-fidelity simulation calls towards the targeted optimum.

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