Network analysis of inflammatory responses to sepsis by neutrophils and peripheral blood mononuclear cells

ABSTRACT Staphylococcal exotoxins (SE) and lipopolysaccharide (LPS) stimulate cells of the immune system to produce proinflammatory cytokines and chemokines which mediate septic shock and acute lung inflammation. A coculture of human peripheral blood mononuclear cells (PBMC) and pulmonary A549 epithelial cells was used to investigate inflammatory responses triggered by staphylococcal enterotoxin B (SEB), toxic shock syndrome toxin 1, and LPS. The levels of interleukin 1β (IL-1β), IL-6, gamma interferon-inducible protein 10, monocyte chemotactic protein 1 (MCP-1), macrophage inflammatory protein 1α, and RANTES were enhanced by 3.8-, 4.2-, 3.1-, 8.9-, 2-, and 2.9-fold, respectively, in cocultures of SEB-stimulated cells compared to in SEB-stimulated PBMC. In LPS-stimulated cocultures, only MCP-1 and RANTES levels were increased. These data suggest that the modulation of specific cytokines and chemokines is dependent on the stimulus and that there is bidirectional interaction between PBMC and lung epithelial cells to influence the immune response to these different stimuli.

Download Full-text

Identification of hub genes in peripheral blood mononuclear cells for the diagnosis of hepatocellular carcinoma using a weighted gene co‑expression network analysis

Experimental and Therapeutic Medicine ◽

10.3892/etm.2020.8736 ◽

2020 ◽

Vol 20 (2) ◽

pp. 890-900

Author(s):

Zi Ye ◽

Zhirui Zeng ◽

Yiyi Shen ◽

Zubing Chen

Keyword(s):

Hepatocellular Carcinoma ◽

Network Analysis ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Hub Genes ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

Download Full-text

Up-regulated lncRNA-PVT1 Expression in Peripheral Blood Mononuclear Cells of Patients with Coronary Artery Disease is Correlated with Decreased Interleukin-10 Production

10.21203/rs.3.rs-50028/v1 ◽

2020 ◽

Author(s):

Behnoosh Miladpour ◽

Atefeh Seghatoleslam ◽

mehdi kalani ◽

Mehran Erfani ◽

peyman Nowrouzi-Sohrabi

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Inflammatory Responses ◽

Peripheral Blood Mononuclear ◽

Cross Sectional ◽

Blood Mononuclear Cells ◽

Artery Disease

Abstract Background: Plasmacytoma variant translocation 1 (PVT1) is a newly discovered long non-coding RNA (lncRNA), and it has not been previously studied in the inflammatory responses of peripheral blood mononuclear cells (PBMCs) of patients with coronary artery disease (CAD). Methods: This cross-sectional study was conducted in 15 CAD patients and 15 non-CAD (NCAD) individuals. PVT1 expression in PBMCs of the participants was measured, using real-time PCR. Interleukin (IL)-10, IL-22 and MMP-9 in the plasma and supernatant of the cultured PBMCs in the presence or absence of lipopolysaccharide (LPS) was assessed, using flowcytometry and ELISA.Results: An increased expression of PVT1 was observed in untreated PBMCs of CAD patients compared to the NCAD group. There was a significant up-regulation of PVT1 after LPS treatment in PBMCs of both groups. Plasma matrix metalloproteinase-9 (MMP-9) levels were found to be higher in CAD patients compared to the controls. The level of IL-10 and IL-22 production from the non-treated PBMCs of CAD was significantly lower compared to the NCAD group. In the total examined population, PVT1 expression was negatively correlated with IL-10 secretion. The results also showed a significant negative correlation between PVT1 expression and IL-10 produced by untreated cells. Conclusions: PVT1 expression is increased in PBMCs of CAD patients and this increased expression could be associated with decreased IL-10 production from PBMCs of these patients.

Download Full-text

Protective Effect of Silibinin on Lipopolysaccharide-Induced Inflammatory Responses in Equine Peripheral Blood Mononuclear Cells, an In Vitro Study

Animals ◽

10.3390/ani10112022 ◽

2020 ◽

Vol 10 (11) ◽

pp. 2022

Author(s):

Enrico Gugliandolo ◽

Rosalia Crupi ◽

Vito Biondi ◽

Patrizia Licata ◽

Salvatore Cuzzocrea ◽

...

Keyword(s):

Protective Effect ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Inflammatory Responses ◽

Mammalian Species ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells ◽

Anti Inflammatory

Although inflammation is an important physiological response, it plays a prominent role in several diseases across the mammalian species. In horses, in particular, inflammation secondary to bacterial infection or translocation is one of the most frequent causes of morbidity and mortality. Research in new molecules with anti-inflammatory and immunomodulatory proprieties and safe use profile is constantly an active field; natural compounds are an important source of molecules with peculiar properties such as antioxidants, anti-inflammatory and immune modulating. Silibinin, a natural polyphenolic flavonoid, extracted from plant milk thistle, Silybum marianum, has been reported to have actions such as antioxidant immunomodulatory and anti-inflammatory. The aim of this study was to test the effect of silibinin on lipopolysaccharide (LPS)-induced inflammatory response in equine peripheral blood mononuclear cells (PBMCs). Our results showed the protective effect of silibinin 10 μM and 50 μM in equine PBMCs stimulated with LPS. Silibilinin was able to prevent the LPS induced increased levels of TNF-α, IL-1β, IL-6 and IL-8. The results from this study on LPS-stimulated equine PBMCs showed that silibinin could be a useful pharmacological approach in treatment or prevention of several inflammatory conditions in horse.

Download Full-text

Associations between dietary patterns and gene expression pattern in peripheral blood mononuclear cells: a cross-sectional study

10.1101/2020.01.25.20018465 ◽

2020 ◽

Author(s):

Jacob J. Christensen ◽

Stine M. Ulven ◽

Magne Thoresen ◽

Kenneth Westerman ◽

Kirsten B. Holven ◽

...

Keyword(s):

Gene Expression ◽

Network Analysis ◽

Peripheral Blood Mononuclear Cells ◽

Dietary Patterns ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Expression Profiles ◽

Gene Clusters ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

AbstractBackgroundDiet may alter gene expression in immune cells involved in cardio-metabolic disease susceptibility. However, we still lack a robust understanding of the association between diet and immune cell-related gene expression in humans.ObjectiveOur objective was to examine the associations between dietary patterns (DPs) and gene expression profiles in peripheral blood mononuclear cells (PBMCs) in a population of healthy, Norwegian adults.MethodsWe used factor analysis to define a posteriori DPs from food frequency questionnaire-based dietary assessment data. In addition, we derived interpretable features from microarray-based gene expression data (13 967 transcripts) using two algorithms: CIBERSORT for estimation of cell subtype proportions, and weighted gene co-expression network analysis (WGCNA) for cluster discovery. Finally, we associated DPs with either CIBERSORT-predicted PBMC leukocyte distribution or WGCNA gene clusters using linear regression models. All analyses were gender-stratified (n = 130 women and 105 men).ResultsWe detected three DPs that broadly reflected Western, Vegetarian, and Low carbohydrate diets. CIBERSORT-predicted percentage of monocytes associated strongly and negatively with the Vegetarian DP in both women and men. For women, the Vegetarian DP associated most strongly with a large gene cluster consisting of 600 genes mainly involved in regulation of DNA transcription. For men, the Western DP inversely associated most strongly with a smaller cluster of 36 genes mainly involved in regulation of metabolic and inflammatory processes. In subsequent protein-protein interaction network analysis, the most important driver genes within these WGCNA gene clusters seemed to physically interact in biological networks.ConclusionsDPs may affect percentage monocytes and regulation of key biological processes within the PBMC pool. Although the present findings are exploratory, our analysis pipeline serves a useful framework for studying the association between diet and gene expression.

Download Full-text