Characterization of Worldwide Olive Germplasm Banks of Marrakech (Morocco) and Córdoba (Spain): Towards management and use of olive germplasm in breeding programs

Cacao breeding programs focus in obtaining cacao varieties with important traits to both producers and consumers. A vital step for plant breeders is the characterization of the genetic material kept in germplasm banks in order to describe its level of diversity, and find interesting traits both morphologically and molecularly. In this way, the investigator will monitor how useful the accessions could be, determine duplications, and manage the bank appropriately. In this study, we aimed to test: the potential of ISSR (Interspread Sequence Repeats) markers to differentiate 46 cacao accessions maintained in Tingo Maria - Perú, and the similarity relationships between the accessions. In this research, the results showed that ISSR, despite its dominance nature, could establish eye-catching associations, such as, the grouping of Trinitario accessions into a common cluster

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A New Breeding Strategy towards Introgression and Characterization of Stay-Green QTL for Drought Tolerance in Sorghum

Agriculture ◽

10.3390/agriculture11070598 ◽

2021 ◽

Vol 11 (7) ◽

pp. 598

Author(s):

Nasrein Mohamed Kamal ◽

Yasir Serag Alnor Gorafi ◽

Hanan Abdeltwab ◽

Ishtiag Abdalla ◽

Hisashi Tsujimoto ◽

...

Keyword(s):

Phenotypic Expression ◽

Phenotypic Selection ◽

Breeding Strategy ◽

Stay Green ◽

Breeding Programs ◽

The Public ◽

Drought Tolerant ◽

Selection Step ◽

The Cost

Several marker-assisted selection (MAS) or backcrossing (MAB) approaches exist for polygenic trait improvement. However, the implementation of MAB remains a challenge in many breeding programs, especially in the public sector. In MAB introgression programs, which usually do not include phenotypic selection, undesired donor traits may unexpectedly turn up regardless of how expensive and theoretically powerful a backcross scheme may be. Therefore, combining genotyping and phenotyping during selection will improve understanding of QTL interactions with the environment, especially for minor alleles that maximize the phenotypic expression of the traits. Here, we describe the introgression of stay-green QTL (Stg1–Stg4) from B35 into two sorghum backgrounds through an MAB that combines genotypic and phenotypic (C-MAB) selection during early backcross cycles. The background selection step is excluded. Since it is necessary to decrease further the cost associated with molecular marker assays, the costs of C-MAB were estimated. Lines with stay-green trait and good performance were identified at an early backcross generation, backcross two (BC2). Developed BC2F4 lines were evaluated under irrigated and drought as well as three rainfed environments varied in drought timing and severity. Under drought conditions, the mean grain yield of the most C-MAB-introgression lines was consistently higher than that of the recurrent parents. This study is one of the real applications of the successful use of C-MAB for the development of drought-tolerant sorghum lines for drought-prone areas.

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Genetic diversity of table grape based on morphoagronomic traits

Scientia Agricola ◽

10.1590/s0103-90162011000100007 ◽

2011 ◽

Vol 68 (1) ◽

pp. 42-49 ◽

Cited By ~ 14

Author(s):

Patrícia Coelho de Souza Leão ◽

Cosme Damião Cruz ◽

Sérgio Yoshimitsu Motoike

Keyword(s):

Genetic Diversity ◽

Clustering Analysis ◽

Germplasm Collection ◽

Table Grape ◽

Cluster Method ◽

Breeding Programs ◽

Method Analysis ◽

Vitis Spp ◽

Grape Cultivars

The conservation and characterization of grape (Vitis spp) genetic resources in germplasm banks have been the basis of its use in breeding programs that result in development of new cultivars. There are at least 10,000 grape cultivars kept in germplasm collection. The genetic diversity in 136 table grape accessions from the state of Bahia, Brazil, was evaluated. Continuous and discrete morphoagronomic traits were assessed. The clustering analysis by the Tocher otimization method resulted in 30 clusters (considering continuous morphoagronomic traits), and 9 clusters (taking into consideration multicategorical traits). There was no agreement between clusters obtained by both, continuous or discrete phenotypic descriptors, independent of the cluster method analysis used. A satisfactory genetic variability among the table grape accessions was observed.

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Ampelographic characterization of white grapevine cul-tivars (Vitis vinifera L.) grown in Palestine

مجلة جامعة فلسطين التقنية للأبحاث ◽

10.53671/pturj.v3i1.34 ◽

2015 ◽

Vol 3 (1) ◽

pp. 1-11

Author(s):

Rezq Basheer-Salimia

Keyword(s):

Genetic Diversity ◽

Genetic Distance ◽

Germplasm Conservation ◽

Genetic Distances ◽

Vitis Vinifera L ◽

Similarity Matrix ◽

Breeding Programs ◽

Local Varieties ◽

Synonymies And Homonymies

Abstract: In Palestine, grape culture consists of ecotypes and cultivars (also called local varieties), for which a large number of homonymous and synonymous designations exist as well as misnaming of cultivars. The present study is the first report using detailed ampelographic characterizations (39 informative traits) to assess genetic diversity and detect similarities among sixteen accessions collected from putative diverse grape genotypes In general, 30 descriptors presented highly and satisfactory divergent genotypes, whereas the remaining traits showed no or very little ampelographic variation. Based on the similarity matrix and the resulting dendrogram of these ampelographic data, distinguishable genotypes as well as some cases of synonymies and homonymies clearly exist. A synonymy case seemed to be in four genotypes including Jandali-Mfarad, Jan-dali-Mrazraz, Jandali, and Hamadani-Mattar, which indeed showed genetic distances of less than 0.5, sug-gesting their relatedness, and the possibility that they are the same genotype, but with different names. In addition, homonym cases also occur in the following pairs of “Marawi’s, Hamadani’s, and Zaini’s genotypes, in which each pair seems to be two distinctive genotypes. Finally, among the 16 examined genotypes, the Zaini-Baladi genotype tended to show the highest genetic distance values from the others and thus could be potentially incorporated into any further local or regional breeding programs as well as germplasm conservation.

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Vernalization response in spring oilseed rape (Brassica napus L.) cultivars

Canadian Journal of Plant Science ◽

10.4141/cjps94-054 ◽

1994 ◽

Vol 74 (2) ◽

pp. 275-277 ◽

Cited By ~ 10

Author(s):

L. A. Murphy ◽

R. Scarth

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Vernalization Response ◽

Brassica Napus L ◽

Early Maturity ◽

Breeding Programs ◽

Cumulative Response ◽

Cold Requirement ◽

Key Words:Brassica

Early maturity is a major objective of oilseed rape (Brassica napus L.) breeding programs in western Canada. Maturity of crops is influenced by time of initiation and flowering. The presence of a vernalization requirement affects plant development by delaying floral initiation until the cold requirement of the plant has been satisfied. Five spring oilseed rape cultivars were screened for their response to vernalization. Vernalization treatments consisted of exposure of germinated seeds to 0–42 d at 4 °C. Plants were assessed under a 20-h photoperiod. In general, there was a cumulative response to vernalization, with a decrease in days to each developmental stage as exposure to 4 °C was increased. Vernalization treatment of 6 d at 4 °C was sufficient to decrease both the days to first flower and the final leaf number. The characterization of vernalization response is of interest because variation in flowering time in response to year-to-year variations in the environment could result. Key words:Brassica napus, canola, oilseed rape, vernalization

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Genetic characterization of the 28 maize landraces in Paraná State

Semina Ciências Agrárias ◽

10.5433/1679-0359.2017v38n4p1715 ◽

2017 ◽

Vol 38 (4) ◽

pp. 1715 ◽

Cited By ~ 1

Author(s):

Sara Regina Silvestrin Rovaris ◽

Pedro Mário de Araújo ◽

Deoclécio Domingos Garbuglio ◽

Cleber Vinicius Guiaretta de Azevedo ◽

Cássio Egídio Cavenaghi Prete

Keyword(s):

Recurrent Selection ◽

Agronomic Traits ◽

Block Design ◽

Breeding Programs ◽

Randomized Block Design ◽

Fixed Model ◽

Maize Landraces ◽

Ear Height ◽

Female Flowering

The characterization of maize landraces is extremely important in breeding programs for use of these genotypes as sources of genetic variability. The objective of this study was to quantitatively characterize 28 populations of maize landraces from the state of Paraná using the estimates of the effects of varieties and heterosis parents and the general combining ability, thereby assessing the main agronomic traits. In the crop of 2008/09, 56 inter-varietal hybrids, obtained through a topcross, 28 populations of maize landraces and three check varieties were evaluated for female flowering (FF), plant height (PH), ear height (EH) and grain mass (GY). The treatments were evaluated in a randomized block design, with two replications, at three Paraná State locations: the Experimental Center of the Agronomic Institute of Paraná in Londrina (IAPAR) and the experimental units of Pato Branco and Ponta Grossa. The data were submitted to an analysis of variance, considering a fixed model for genotypes and a random model for environments; the averages grouped by the Scott-Knott test, along with intersections of topcrosses, were analyzed according to a readapted model proposed by Oliveira et al. (1997). According to estimates of the parental effects, the GI 133 population showed the most promising estimates for all characteristics. The GI 088 and GI 173 populations stood out with promising estimates of the effects of heterosis. The conclusion is that the populations GI 133 and GI 173 may be indicated for recurrent selection programs or participation in obtaining composites.

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Phenotypic and molecular characterization of a set of tropical maize inbred lines from a public breeding program in Brazil

BMC Genomics ◽

10.1186/s12864-021-08127-7 ◽

2022 ◽

Vol 23 (1) ◽

Author(s):

Sirlene Viana de Faria ◽

Leandro Tonello Zuffo ◽

Wemerson Mendonça Rezende ◽

Diego Gonçalves Caixeta ◽

Hélcio Duarte Pereira ◽

...

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Inbred Lines ◽

Breeding Program ◽

Tropical Maize ◽

Maize Breeding ◽

Breeding Programs ◽

The Public ◽

Maize Inbred Lines

Abstract Background The characterization of genetic diversity and population differentiation for maize inbred lines from breeding programs is of great value in assisting breeders in maintaining and potentially increasing the rate of genetic gain. In our study, we characterized a set of 187 tropical maize inbred lines from the public breeding program of the Universidade Federal de Viçosa (UFV) in Brazil based on 18 agronomic traits and 3,083 single nucleotide polymorphisms (SNP) markers to evaluate whether this set of inbred lines represents a panel of tropical maize inbred lines for association mapping analysis and investigate the population structure and patterns of relationships among the inbred lines from UFV for better exploitation in our maize breeding program. Results Our results showed that there was large phenotypic and genotypic variation in the set of tropical maize inbred lines from the UFV maize breeding program. We also found high genetic diversity (GD = 0.34) and low pairwise kinship coefficients among the maize inbred lines (only approximately 4.00 % of the pairwise relative kinship was above 0.50) in the set of inbred lines. The LD decay distance over all ten chromosomes in the entire set of maize lines with r2 = 0.1 was 276,237 kb. Concerning the population structure, our results from the model-based STRUCTURE and principal component analysis methods distinguished the inbred lines into three subpopulations, with high consistency maintained between both results. Additionally, the clustering analysis based on phenotypic and molecular data grouped the inbred lines into 14 and 22 genetic divergence clusters, respectively. Conclusions Our results indicate that the set of tropical maize inbred lines from UFV maize breeding programs can comprise a panel of tropical maize inbred lines suitable for a genome-wide association study to dissect the variation of complex quantitative traits in maize, mainly in tropical environments. In addition, our results will be very useful for assisting us in the assignment of heterotic groups and the selection of the best parental combinations for new breeding crosses, mapping populations, mapping synthetic populations, guiding crosses that target highly heterotic and yielding hybrids, and predicting untested hybrids in the public breeding program UFV.

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Mapping and Characterization QTLs for Phenological Traits in Seven Pedigree-connected Peach Families

10.21203/rs.3.rs-75550/v1 ◽

2020 ◽

Author(s):

Zena Rawandoozi ◽

Timothy Hartmann ◽

Silvia Carpenedo ◽

Ksenija Gasic ◽

Cassia da Silva Linge ◽

...

Keyword(s):

Prunus Persica ◽

Phenotypic Variance ◽

Breeding Programs ◽

Large Tree ◽

Early Seedling ◽

Sib Families ◽

Functional Alleles ◽

Genomic Regions ◽

Marker Identification

Abstract BackgroundEnvironmental adaptation and expanding harvest seasons are primary goals of most peach [Prunus persica (L.) Batsch] breeding programs. Breeding perennial crops is a challenging task due to their long breeding cycles and large tree size. Pedigree-based analysis using pedigreed families followed by haplotype construction creates a platform for QTL and marker identification, validation, and the use of marker-assisted selection in breeding programs.ResultsPhenotypic data of seven F1 low to medium chill full-sib families were collected over two years at two locations and genotyped using the 9K SNP Illumina array. Three QTLs were discovered for bloom date (BD) and mapped on linkage group 1 (LG1) (172 – 182 cM), LG4 (48 – 54 cM), and LG7 (62 – 70 cM), explaining 17-54%, 11-55%, and 11-18% of the phenotypic variance, respectively. The QTL for ripening date (RD) and fruit development period (FDP) on LG4 was co-localized at the central part of LG4 (40 - 46 cM) and explained between 40-75% of the phenotypic variance. Haplotype analyses revealed SNP haplotypes and predictive SNP marker(s) associated with desired QTL alleles and the presence of multiple functional alleles with different effects for a single locus for RD and FDP.ConclusionsA multiple pedigree-linked families approach validated major QTLs for the three key phenological traits which were reported in previous studies across diverse materials, geographical distributions, and QTL mapping methods. Haplotype characterization of these genomic regions differentiates this study from the previous QTL studies. Our results will provide the peach breeder with the haplotypes for three BD QTLs and one RD/FDP QTL for the creation of predictive DNA-based molecular marker tests to select parents and/or seedlings that have desired QTL alleles and cull unwanted genotypes in early seedling stages.

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VARIABILIDADE GENÉTICA UTILIZANDO DESCRITORES MORFOLÓGICOS PARA ACESSOS DE Capsicum spp. PARA FINS ORNAMENTAIS

Nativa ◽

10.31413/nativa.v7i6.7984 ◽

2019 ◽

Vol 7 (6) ◽

pp. 675

Author(s):

Petterson Baptista da Luz ◽

Alessandro Aparecido Brito dos Santos ◽

Valdete Campos Ambrosio

Keyword(s):

Genetic Variability ◽

Ornamental Plants ◽

Growth Potential ◽

Breeding Programs ◽

Canonical Correlations ◽

Plant Parts ◽

Potted Plants ◽

Capsicum Spp ◽

Biometric Templates

O mercado de pimentas para fins ornamentais é um setor recém explorado, mas com grande potencial de crescimento no ramo de floricultura e paisagismo. As pimenteiras que possuem folhagem variegada, porte baixo, frutos com coloração variada em seus diferentes estádios de maturação, contrastando com as folhagens, já são utilizadas como plantas ornamentais, cultivadas e comercializadas em vasos. O presente trabalho teve como objetivo avaliar a variabilidade genética visando o potencial ornamental de acessos de Capsicum spp. através de modelos biométricos. Foram utilizados 55 acessos de Capsicum spp, e a caracterização morfoagronômica dos acessos foi realizada avaliando diferentes partes da planta. O terceiro par de correlações canônicas, com correlação de 0,51, associa plantas com área do dossel compacto-densa, com menor comprimento e largura de dossel e de pequeno porte, associado com folhagens estreitas e de pecíolo grande. Esta associação é vantajosa para ornamentação. Ficou evidenciada a presença de variabilidade genética na população, o que é bastante favorável ao melhoramento na medida em que a heterogeneidade genética possibilita a obtenção de ganhos por meio de seleção.Palavras-chave: pimenta; modelos biométricos; correlações canônicas; análise de trilha. GENETIC VARIABILITY FOCUSED ON ORNAMENTAL POTENTIAL OF A Capsicum spp. ACCESSION ABSTRACT: Pepper market for ornamental purposes is recently exploited; however, it has a great growth potential in floriculture and landscaping. Pepper with variegated foliage, small size, and varied fruit coloration at different ripening stages contrasting with leaves have already been used as ornamental plants cultivated and purchased as potted plants. This study aimed to evaluate genetic variability of Capsicum spp. focusing on its ornamental potential by means of biometric templates. Fifty-five Capsicum spp. accessions were used and a morpho-agronomic characterization of the accessions were performed by evaluating different plant parts. The third canonical correlations are 0.51 and it associates compact-dense, small, shorter length and width canopy plants with narrow foliage and large petiole, which is advantageous for ornamentation. Genetic variation was evidenced in the population being very favorable for plant breeding programs once genetic heterogeneity enables obtaining gains through selections.Keywords: pepper; biometric templates; canonic correlations; trail analysis.

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Characterization of the Common japonica-Originated Genomic Regions in the High Yielding Varieties Developed from Inter-Subspecific Crosses in Rice (Oryza sativa L.)

10.20944/preprints202004.0428.v1 ◽

2020 ◽

Author(s):

Jeonghwan Seo ◽

So-Myeong Lee ◽

Jae-Hyuk Han ◽

Na-Hyun Shin ◽

Yoon Kyung Lee ◽

...

Keyword(s):

Molecular Breeding ◽

Snp Markers ◽

Yield Potential ◽

Breeding Programs ◽

Wx Gene ◽

High Yielding Varieties ◽

Indica And Japonica Subspecies ◽

Genomic Regions ◽

Further Development

The inter-subspecific crossing between indica and japonica subspecies in rice have been utilized to improve yield potential in temperate rice. In this study, a comparative study of the genomic regions in the eight high yielding varieties (HYVs) was conducted with those of the four non-HYV varieties. NGS mapping on the Nipponbare reference genome identified a total of 14 common genomic regions of japonica-originated alleles. Interestingly, the HYVs shared the japonica-originated genomic regions on the nine chromosomes, although they were developed from different breeding programs. A panel of 94 varieties was classified into four varietal groups with the 39 SNP markers from 39 genes residing the japonica-originated genomic regions and 16 additional trait-specific SNPs. As expected, the japonica originated genomic regions were present only in JAP and HYV groups with exceptions for Chr4-1 and Chr4-2. The Wx gene located within Chr6-1 was present in HYV and JAP variety groups, while the yield-related genes were conserved as indica alleles in HYVs. The japonica-originated genomic regions and alleles shared by HYVs can be employed in molecular breeding programs for further development of HYVs in rice.

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