Reproductive events and respective faecal androgen metabolite concentrations in captive male roan antelope (Hippotragus equinus)

Understanding the reproductive biology of the roan antelope (Hippotragus equinus) (É. Geoffroy Saint-Hilaire, 1803) is crucial to optimise breeding success in captive breeding programmes of this threatened species. In this study, the pattern of faecal androgen metabolite (fAM) production related to reproductive events (calving or birthing, mating, gestation, and lactation), sexual behaviours as well as environmental cues were studied in captive adult male roan antelope. Faecal sample collection and behavioural observations were carried out from August 2017 to July 2018 for three reproductive males participating in a conservation breeding programme at the Lapalala Wilderness Nature Reserve in South Africa. As a prerequisite, the enzyme immunoassay used in this study was biologically validated for the species by demonstrating a significant difference between fAM concentrations in non-breeding adults, breeding adults and juvenile males. Results revealed that in adults males, the overall mean fAM levels were 73% higher during the breeding period compared to the non-breeding periods, and 85% higher when exclusively compared to the lactation/gestation periods, but only 5.3% higher when compared to the birthing period. Simultaneously, fAM concentrations were lower during the wet season compared to the dry season, increasing with a reduction in photoperiod. With the exception of courtship, frequencies of sexual behaviours monitored changed in accordance with individual mean fAM concentrations in male roan antelope, the findings suggest that androgen production varies with the occurrence of mating activity and may be influenced by photoperiod but not with rainfall.

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Theileriosis (Cytauxzoonosis) in Roan antelope (Hippotragus equinus): Field exposure to infection and identification of potential vectors

Onderstepoort Journal of Veterinary Research ◽

10.4102/ojvr.v79i1.367 ◽

2012 ◽

Vol 79 (1) ◽

Cited By ~ 10

Author(s):

Johan C.A. Steyl ◽

Leon Prozesky ◽

Wilhelm H. Stoltsz ◽

John A. Lawrence

Keyword(s):

Salivary Glands ◽

Tick Species ◽

Rhipicephalus Appendiculatus ◽

Dna Probe ◽

Field Exposure ◽

Feeding Site ◽

Pcr Screening ◽

Significant Difference ◽

Roan Antelope ◽

Hippotragus Equinus

Four hand-reared, naïve roan antelope, 4 months of age, were exposed to naturally infected pasture on a game farm in Mpumalanga Province, South Africa, where roan are known to die from theileriosis. Various clinical parameters were recorded during this period. The predominant ticks parasitising these animals at the time (January to February), were Rhipicephalus appendiculatus and Rhipicephalus evertsi evertsi adults. After a period of 5 weeks the animals developed signs of clinical theileriosis and were treated with buparvaquone to prevent mortality. Primary hyperplasia of the local draining lymph nodes (Lnn. anorectales) near the feeding site of adult R. evertsi evertsi indicated possible transmission of Theileria sp. (sable) by this tick species. After recovery from theileriosis, these animals were confirmed carriers of Theileria sp. (sable) by PCR (polymerase chain reaction) and DNA probe analysis. Laboratory-bred larvae and nymphs of R. evertsi evertsi and R. appendiculatus respectively, were fed on the ears of these roan antelope. Salivary glands from moulted and prefed adult ticks of each species were dissected and stained for Theileria spp., and the PCR and DNA probe applied to a representative batch of dissected glands. R. appendiculatus adults collected from grass in infected camps were also dissected after prefeeding them on rabbits. Salivary glands of both tick species showed infected acini on staining and were also positive for Theileria sp. (sable) only, on multiprotozoal PCR-screening analysis. There was no statistical significant difference between the infection rate and the intensity of infection between the two tick species. R. appendiculatus ticks collected from grass were also PCR-positive for Theileria sp. (sable).

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Population Structure of Ungulates in Waterberg National Park, Namibia

Vestnik Zoologii ◽

10.2478/vzoo-2019-0004 ◽

2019 ◽

Vol 53 (1) ◽

pp. 31-46 ◽

Cited By ~ 1

Author(s):

E. Kasiringua ◽

Ş. Procheş ◽

G. Kopij

Keyword(s):

Population Structure ◽

National Park ◽

African Buffalo ◽

Wet Season ◽

Sex Structure ◽

Tragelaphus Strepsiceros ◽

White Rhino ◽

Roan Antelope ◽

Greater Kudu ◽

Hippotragus Equinus

Abstract A study on the seasonal variation in population structure of seven (7) ungulate species, African buffalo (Syncerus caffer), eland (Tragelaphus oryx), giraffe (Giraffa camelopardalis), oryx (Oryx gazella), sable (Hippotragus niger), roan antelope (Hippotragus equinus) and the greater kudu (Tragelaphus strepsiceros) was carried out using the field census approach during the wet (February– April) and dry (July–September) season, and a water hole census (September) at seven waterholes in the Waterberg National Park, Namibia. Male warthog, oryx and black rhino on average comprised about 40 % of the population, whereas male sable, roan antelope, eland and buffalo comprised 30 % of the population. Male kudu and male white rhino comprised 20 % of the sex structure of the population, whereas male giraffe comprised more than 50 % of population. Age structure was dominated by adults, with 60 % of the total population in warthog, sable, eland and the white rhino, and 70 % in kudu, roan, buffalo, giraffe and black rhino on average. Oryx had the highest number of adults, with only 10 % of the population comprised of juveniles. Larger herds were observed during the wet season and smaller herds during the dry season. Animals were observed more abundantly in three of the vegetation types (Terminalia sericea — Melhania acuminata vegetation, Terminalia sericea — Thesium megalocarpum, Terminalia sericea — Blepharis integrifolia), with lower abundances in the rock-inhabiting Peltophorum africanum during both seasons.

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De novo whole-genome assembly and resequencing resources for the roan (Hippotragus equinus), an iconic African antelope

G3 Genes|Genome|Genetics ◽

10.1093/g3journal/jkab002 ◽

2021 ◽

Vol 11 (2) ◽

Author(s):

Margarida Gonçalves ◽

Hans R Siegismund ◽

Bettine Jansen van Vuuren ◽

Klaus-Peter Koepfli ◽

Nuno Ferrand ◽

...

Keyword(s):

Captive Breeding ◽

De Novo ◽

Repetitive Sequences ◽

Draft Genome ◽

Whole Genome Sequence ◽

Whole Genome ◽

Single Nucleotide Variants ◽

Captive Breeding Program ◽

Roan Antelope ◽

Hippotragus Equinus

Abstract Roan antelope (Hippotragus equinus) is the second-largest member of the Hippotraginae (Bovidae), and is widely distributed across sub-Saharan mesic woodlands. Despite being listed as "Least Concern" across its African range, population numbers are decreasing with many regional Red List statuses varying between Endangered and Locally Extinct. Although the roan antelope has become an economically-important game species in Southern Africa, the vast majority of wild populations are found only in fragmented protected areas, which is of conservation concern. Genomic information is crucial in devising optimal management plans. To this end, we report here the first de novo assembly and annotation of the whole-genome sequence of a male roan antelope from a captive-breeding program. Additionally, we uncover single-nucleotide variants (SNVs) through re-sequencing of five wild individuals representing five of the six described subspecies. We used 10X Genomics Chromium chemistry to produce a draft genome of 2.56 Gb consisting of 16,880 scaffolds with N50 = 8.42 Mb and a BUSCO completeness of 91.2%. The draft roan genome includes 1.1 Gbp (42.2%) repetitive sequences. De novo annotation identified 20,518 protein-coding genes. Genome synteny to the domestic cow showed an average identity of 92.7%. Re-sequencing of five wild individuals to an average sequencing depth of 9.8x resulted in the identification of a filtered set of 3.4x106 bi-allelic SNVs. The proportion of alternative homozygous SNVs for the individuals representing different subspecies, as well as differentiation as measured by PCA, were consistent with expected divergence from the reference genome and among samples. The roan antelope genome is a valuable resource for evolutionary and population genomic questions, as well as management and conservation actions.

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ANALISIS TINGKAT PEMAHAMAN MAHASISWA AKUNTANSI TERHADAP KONSEP DASAR AKUNTANSI (Studi Empiris pada Mahasiswa Akuntansi di Universitas Bung Hatta Padang)

Jurnal Kajian Akuntansi dan Auditing ◽

10.37301/jkaa.v0i0.5379 ◽

2013 ◽

Author(s):

Ethika Ethika ◽

Ade Saputra

Keyword(s):

High School ◽

Social Studies ◽

Hypothesis Testing ◽

Vocational Education ◽

Formal Education ◽

Accounting Students ◽

Sample Collection ◽

Significant Difference ◽

Basic Concepts ◽

Education Of Students

This study has several objectives first prove a significant difference in understanding of the assets, liabilities and capital between the students from SMK Accounting Department, High School and Madrasah Aliyah Department of Public IPS IPS majors. Prior to hypothesis testing performed prior sample collection is 68 students who have graduated from vocational secondary education level majoring in Accountancy, SMA study of majoring of IPS science Madrasah Aliyah common in college majoring in social studies at the Bung Hatta University of Padang. Type of data used are obtained through deployment of primary questionnaire. Variable study is a basic understanding of accounting concepts consisting of cash, liabilities and capital, while the distinguishing dimension is the level of formal education of students in the past. The process of hypothesis testing is done by using a statistical test that is using the Kruskal Walls. Based on the results of testing the first hypothesis of no difference in the understanding of accounting students from vocational education majoring in accounting, SMA students and Madrasah Aliyah major of IPS science. in understanding the basic concepts of accounting view of the assets. The second hypothesis found differences in the understanding of accounting students from vocational education majoring in accounting, high school social studies department, and public Madrasah Aliyah Department the major of IPS science of the basic concepts of accounting in view of the obligation. The third hypothesis found no differences in accounting students' understanding of vocational education majoring in accounting, SMA student, and Madrasah Aliyah general social studies department of the basic concepts of accounting view of capital.

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Effects of collection zones and storage on hatchability and survival of feral helmeted guineafowl (Numida meleagris galleata pallas) eggs

Zoologist (The) ◽

10.4314/tzool.v18i1.13 ◽

2021 ◽

Vol 18 (1) ◽

pp. 74-81

Author(s):

A.A. Yusuf ◽

O.A. Jayeola ◽

I.O.O. Osunsina ◽

G.A. Dedeke

Keyword(s):

National Park ◽

Captive Breeding ◽

Storage Systems ◽

Ecological Zones ◽

Numida Meleagris ◽

Significant Difference ◽

In Captivity ◽

Protein Supply ◽

And Storage ◽

Kainji Lake

The desire to shore up the shortfall in protein supply, the increased awareness of the importance of cholesterol free animal protein, like guineafowl, and the need to conserve their wild genes, have necessitated studies on improved captive breeding of feral helmeted guineafowl. Hence, this study was aimed at determining the effects of ecological zones and storage systems on the guineafowl eggs. Guineafowl eggs (n=214), were collected from identified and monitored-nests within the Kainji Lake National Park (KLNP) and Old Oyo National Park (OONP). Out of 91 eggs collected from KLNP, 32 and 38 were stored at room temperature (RT-21-25°C) and refrigerator (RF-17-20°C), respectively for five days prior to incubation while 21 eggs were not stored (NS-27-29°C), and out of 123 collected from OONP, 70 and 19 were stored for five days prior to incubation at (RT-21-25°C) and (RF-17-20°C), respectively while 34 eggs were (NS- 27-29°C). Prior to incubation, eggs were weighed, the height and width were measured. The process was repeated after incubation for unhatched eggs. Candling was done three days before hatching at day 29 of egg incubation. Embryo status of unhatched eggs was determined by cracking the eggs. Descriptive and inferential statistics were used to analyse the data. The NS eggs from Kainji (7.60%) and Oyo (2.01%) had the lowest percentage shrinkage in weight across the three storage systems. The order was reversed in height with RF eggs from Kainji (0.96%) and Oyo (0.46%) having lowest. The least shrinkage in width of eggs from Oyo was recorded in the RF eggs (0.00%) and in NS (0.59%) from Kainji. Eggs candling showed that presumed fertile (opaque) was highest (69.10%) in NS eggs followed by RT (45.00%). There was no significant difference (p>0.05) between the hatchability of eggs from the two parks though hatchability (7.60%) of Kainji eggs was higher than those of Oyo (7.40%). Further check on fertilization after incubation showed that RT (37.50%) eggs from Kainji were fertilized but were unable to hatch alive so also was RF eggs (21.10%). The study showed that the eggs sizes vary with ecozones while size of the eggs and storage systems affects hatchability and survival of feral helmeted guineafowl eggs in captivity. Keywords: Guineafowl; ecozones; storage systems; incubation; candling; hatchability

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Triglycerides, independent of Ferriman Gallwey Score, is a main determinant of free testosterone index in PCOS

F1000Research ◽

10.12688/f1000research.16815.1 ◽

2019 ◽

Vol 8 ◽

pp. 94

Author(s):

Andon Hestiantoro ◽

Putri Deva Karimah ◽

Amalia Shadrina ◽

Budi Wiweko ◽

R. Muharam ◽

...

Keyword(s):

Free Testosterone ◽

Cross Sectional Study ◽

Pcos Patient ◽

Reproductive Age ◽

Bivariate Analysis ◽

Sample Collection ◽

Cross Sectional ◽

Significant Difference ◽

Shbg Level ◽

Metabolic Dysfunctions

Background: Polycystic Ovarian Syndrome (PCOS) is the most common endocrinopathy in women of reproductive age, affecting 5-20% of women worldwide. Hyperandrogenism, as the primary characteristic of PCOS, is not always present in every patient. The hyperandrogenic phenotype of PCOS patients is influenced by both hormonal and metabolic dysfunctions. Therefore, this study aims to determine the correlation between hormone profile, lipid profile, and clinical profile with free testosterone index in subjects with PCOS. Methods: This prospective cross-sectional study was conducted in the Dr. Cipto Mangunkusumo General Hospital between July 2014 and December 2016. The study involved 76 women with PCOS, who were classified into 2 subgroups: 39 subjects in the hyperandrogenism group and 37 subjects in the non-hyperandrogenism group. Each subject underwent physical examination, blood sample collection, and USG examination. Bivariate analysis was done using independent t-tests and Mann Whitney U-tests, while multivariate analysis was done using logistic regression. Results: Triglyceride and testosterone level showed weak (r = 0.232, p = 0.044) and moderate (r = 0.460, p ¡ 0.001) positive correlation with FTI, while SHBG level showed moderate negative correlation (r = -0.483, p ¡ 0.001). Triglyceride was also found to be determinant of hyperandrogenism condition in PCOS patient (OR 0.02, 95% CI 0.00–0.04, p = 0.013). However, there was no significant difference observed between FGS and hyperandrogenism (p = 0.43). Conclusions: Triglycerides, testosterone, and SHBG were associated with hyperandrogenism in PCOS patients, while FGS showed no such association.

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FORAGE PRODUCTION OF SOME LESSER-KNOWN LEUCAENA SPECIES GROWN ON ACID SOIL

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v6n2.2005.p46-51 ◽

2016 ◽

Vol 6 (2) ◽

pp. 46

Author(s):

Nurhayati D. Purwantari

Keyword(s):

Acid Soil ◽

Calcareous Soils ◽

Dry Weight ◽

Forage Production ◽

Fresh Weight ◽

Wet Season ◽

Edible Part ◽

P Content ◽

Significant Difference ◽

Leucaena Species

Leucaena leucocephala leaves are nutritious and palatable for ruminants. However, the plant is poorly adapted on acid soil, water logged, and susceptible to psyllid (Heteropsylla cubana). Leucaena favours soils with pH >5. It performs best on calcareous soils. This study aimed to evaluate adaptability of several Leucaena species on acid soils. The study was conducted at the experimental field in Ciawi, Bogor, altitude 500 m above sea level, rainfall 1500-2000 mm year-1, on Latosol soil with pH 5.2. Leucaena species evaluated were L. leucocephala K28, L. leucocephala (local type), Leucaena KX2 hybrid, L. collinsii, L. trichandra, and L. diversifolia. Experiment was designed in a randomized complete block, four replications. All the seedlings were grown in the nursery for 2.5 months before being transplanted to the field. The plants were grown in row plots of 5 m x 1 m with planting distance 0.5 m and spacing between row plot was 3 m. One unit replicate was 5 m x 21 m. In wet season, the plants were pruned at 50 cm height every 3 months, whilst in dry season pruning was done every 5 months. Parameter measured were plant regrowth and forage production (edible part and non-edible part of the plant), as well as N and P content of the edible part. The result showed that Leucaena KX2 hybrid was superior to other leucaenas. Regrowth of KX2 hybrid was the fastest and produced highest forage. The highest fresh weight forage production for 1 year (four times harvests), i.e. 33.93 t ha-1 year-1 equal to 12.48 t ha- 1 year-1 of dry weight, was obtained from Leucaena KX2 hybrid, whereas the lowest was produced by L. diversifolia, i.e. 3.12 t ha-1 year-1 of fresh weight or 1.01 t ha-1 year-1 of dry weight. Production of non-edible part followed the pattern of production of edible part. N and P content in the edible part of all Leucaena species did not show any significant difference except for L. trichandra which contained the lowest. This study implies that Leucaena KX2 hybrid is adaptable to acid soil; therefore it can be grown in wider areas throughout Indonesia.

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Twelve-Hour Urine Collection Can Be Utilized to Estimate 24-Hour Total Urinary Nitrogen in Critically Trauma Patients

American Journal of Clinical Pathology ◽

10.1093/ajcp/aqz112.001 ◽

2019 ◽

Vol 152 (Supplement_1) ◽

pp. S1-S1

Author(s):

Hsuan-Chieh Liao ◽

Grant O’Keefe ◽

Fred Woo ◽

Lisa Lawrence ◽

Geoffrey Baird

Keyword(s):

Correlation Coefficients ◽

Urine Samples ◽

Trauma Patients ◽

Urine Collection ◽

Sample Collection ◽

Icu Patients ◽

Nitrogen Levels ◽

Significant Difference ◽

Regression Slopes ◽

Urinary Nitrogen

Abstract Patients suffering from trauma, burns, and sepsis are frequently in a hypermetabolic state, and as the majority of urinary nitrogen comes from amino acid metabolism, the determination of total urinary nitrogen (TUN) provides an accurate measurement of all nitrogen excreted in urine and can be used as an indicator of catabolic stress. To evaluate whether time of collection influences the performance of TUN test, 12-hour urine specimens were collected from ICU trauma patients and the 12-hour results were correlated with results from 24-hour collections. Two consecutive 12-hour urine samples were collected (06:00-18:00 hours and 18:00-06:00 hours) from surgical ICU patients. Total urinary nitrogen levels were measured in each 12-hour sample, as well as in a pooled sample (24 hours). Pyrochemiluminescence was used to determine TUN level on an Antek 9000N elemental analyzer. In this approach, the urine sample is completely oxidized at high temperature in a quartz pyrotube. Nitrogen is converted to nitric oxide (·NO), then mixed with O3 (ozone) to form nitrogen dioxide (NO2). Light is emitted and specific wavelengths between 650 and 900 nm were measured in a photomultiplier tube. The measured chemiluminescent emission is specific and proportional to the amount of nitrogen in the sample. Fifty 12-hour urine samples from 16 patients were collected. One patient was excluded from the study due to acute renal injury during the collection period. There was no significant observed circadian effect on measured TUN. The 12-hour TUN (g/total volume) was multiplied by 2 to compare to 24-hour TUN for statistical analysis. There was strong correlation between either day or night 12-hour TUN and corresponding pooled 24-hour TUN, with correlation coefficients ranging from 0.93 to 0.98 and regression slopes ranging from 0.98 to 1.01. No statistically significant difference was found between the 12-hour TUN and 24-hour TUN approaches. A 12-hour TUN collection was overall highly predictive of 24-hour TUN collection and has the advantage of convenience of sample collection and improved clinical efficiency. Serial 12-hour urine collection is therefore preferred for monitoring nitrogen balance and adjusting protein intake for critically ill ICU patients.

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Evaluation of Urine Reagent Strip as a Tool for Routine Diagnosis of Maternal Urogenital Schistosomiasis at Antenatal Clinic Visit in Munyenge, South West Region, Cameroon

BioMed Research International ◽

10.1155/2019/2972630 ◽

2019 ◽

Vol 2019 ◽

pp. 1-7

Author(s):

Godlove Bunda Wepnje ◽

Judith Kuoh Anchang-Kimbi ◽

Leopold Gustave Lehman ◽

Helen Kuokuo Kimbi

Keyword(s):

Health Centre ◽

Antenatal Clinic ◽

Clinic Visit ◽

Functional Health ◽

Egg Load ◽

Sample Collection ◽

Urogenital Schistosomiasis ◽

Filtration Method ◽

Predictive Values ◽

Significant Difference

Urine reagent strip used in detecting microhaematuria has been recommended in pregnancy for diagnosis of urogenital schistosomiasis (UGS) during routine antenatal care (ANC). This study evaluated its sensitivity, specificity, and predictive values in the diagnosis of maternal UGS using filtration method as a reference test. We also assessed the variation in its performance in the diagnosis of UGS using multiple-sample collection. A total of 93 pregnant women reporting for first ANC clinic visit at any of the three functional health care centres (Munyenge Integrated Health Centre, Banga Annex Health Centre, and Trans African Health Centre) were enrolled and followed up for three consecutive monthly visits. Urine samples were observed microscopically for S. haematobium egg using urine filtration and screened for microhaematuria and proteinuria using urine reagent strips. Twenty-two (23.7%) out of the 93 women were diagnosed for UGS, all of whom showed S. haematobium egg excretion during all three visits. There was a significant difference (p<0.001) between the prevalence of S. haematobium infection and the prevalence of microhaematuria. The intensity of infection was significantly higher in microhaematuria-positive women compared with microhaematuria-negative cases. Sensitivity of reagent strip ranged from 54.5 to 59.1%, while specificity was above 98.0% (range: 98.6–100%). The measure of agreement between urine filtration and reagent strip method was substantial (0.61–0.8) irrespective of different sampling periods. Urine reagent strip is a moderately sensitive method in the detection of UGS and will most likely identify women with high egg load burden. Proper diagnosis of schistosomiasis during pregnancy is recommended.

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Seasonality of Mansonia titillans during dam construction, Biritiba-Mirim, São Paulo State, Brazil

Biota Neotropica ◽

10.1590/s1676-06032013000100007 ◽

2013 ◽

Vol 13 (1) ◽

pp. 70-73 ◽

Cited By ~ 2

Author(s):

Frederico Alves D'Avila ◽

Almério de Castro Gomes

Keyword(s):

Early Spring ◽

Dry Season ◽

Southeastern Brazil ◽

Rank Test ◽

Late Winter ◽

Dam Construction ◽

Wet Season ◽

Paulo State ◽

Significant Difference ◽

Wilcoxon Rank Test

A two and a half year survey was conducted at a dam in southeastern Brazil. Shannon Traps were used for sampling. Kruskal-Wallis test showed little relation between rainfall and abundance. The data clearly show three abundance peaks, all of them in the end of the dry season, in consonance with the scarce literature existent. Although Kruskal-Wallis Test did not find a clear preference for the dry season, Pairwise Wilcoxon Rank Test revealed a significant difference between Fall and Spring samples. Ma. titillans population had a peak on late winter/early spring, close to the begin of wet season.

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