scholarly journals Comparative transcriptomics of leaves of five mulberry accessions and cataloguing structural and expression variants for future prospects

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0252246
Author(s):  
Vinay Kumar Baranwal ◽  
Nisha Negi ◽  
Paramjit Khurana
Keyword(s):  
Transcription Factors ◽  
Zinc Fingers ◽  
Algebraic Connectivity ◽  
Future Prospects ◽  
High Quality ◽  
Breeding Programs ◽  
Anatomical Features ◽  
Biochemical Pathways ◽  
Biochemical Compounds ◽  
One To One

Bombyx mori, a monophagous insect, prefers leaves of the certain species of Morus more than others. The preference has been attributed to morphological and anatomical features and biochemical compounds. In the present manuscript a comparison has been made among the transcriptome of leaves of the two preferred cultivated varieties and three wild types species. While assembling, high quality transcriptomes of five genotypes were constructed with a total of 100930, 151245, 89724, 181761 and 102908 transcripts from ML, MN, MS, K2 and V1 samples respectively. Further, to compare them, orthologs were identified from these assembled transcriptome. A total of 22462, 23413, 23685, 24371, 18362, 22326, 20058, 18049, 17567 and 20518 clusters of orthologs were found in one to one comparison in KvsN, KvsL, KvsS, KvsV, LvsN, LvsS, LvsV, NvsS, NvsV, and SvsV respectively. 4236 orthologs with algebraic connectivity of 1.0 were then used to compare and to find out differentially expressed transcripts from all the genotypes. A total of 1037 transcripts expressed that include some of the important morphology, anatomy and biochemical pathways regulating transcription factors (AP2/ERFs and C2H2 Zinc fingers) and signalling components were identified to express differentially. Further, these transcriptomes were used find out markers (SSR) and variants and a total of 1101013, 537245, 970877, 310437, 675772, 338400, 581189, 751477, 514999 and 257107 variants including SNP, MNP, Insertions and deletions were found in one to one comparisons. Taken together, our data could be highly useful for mulberry community worldwide as it could be utilized in mulberry breeding programs.

scholarly journals Pawpaw Variety Development: A History and Future Prospects

2003 ◽  
Vol 13 (3) ◽  
pp. 449-454 ◽  
Author(s):  
R. Neal Peterson
Keyword(s):  
State University ◽  
Foreseeable Future ◽  
Future Prospects ◽  
High Quality ◽  
Breeding Programs ◽  
The Third ◽  
Asimina Triloba ◽  
History Of ◽  
New Crop

The pawpaw (Asimina triloba) is a new crop in the early stages of domestication. Recently commercialization has become feasible with the availability of high quality varieties. The history of pawpaw varieties is divided into three periods: 1900-50, 1950-85, and 1985 to the present. The history before 1985 was concerned primarily with the discovery of superior selections from the wild but experienced a serious break in continuity around 1950. The third period has been characterized by greater developmental activity. Larger breeding programs have been pursued, regional variety trials initiated, a germplasm repository established, and a formal research program at Kentucky State University (KSU) instituted. Future breeding will likely rely on dedicated amateurs with the education and means to conduct a 20-year project involving the evaluation of hundreds of trees. For the foreseeable future, governments and universities will not engage in long-term pawpaw breeding.

Mentoring High-Impact Undergraduate Research Experiences

2022 ◽  
Vol 22 (1) ◽  
pp. 17-21
Author(s):  
Jessie L. Moore ◽  
Angela Myers ◽  
Hayden McConnell
Keyword(s):  
Research Team ◽  
Undergraduate Research ◽  
High Impact ◽  
English Studies ◽  
High Quality ◽  
Research Experiences ◽  
Undergraduate Research Experiences ◽  
One To One

Abstract This article illustrates the Ten Salient Practices of Undergraduate Research Mentors with examples for English studies. The authors include both one-to-one and research-team examples, recognizing that although much English scholarship is solitary, peers and near peers play key roles in high-quality, mentored undergraduate research experiences.

scholarly journals A Review on Additive Manufacturing of Micromixing Devices

Micromachines ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 73
Author(s):  
Marina Garcia-Cardosa ◽  
Francisco-Javier Granados-Ortiz ◽  
Joaquín Ortega-Casanova
Keyword(s):  
Life Cycle ◽  
Additive Manufacturing ◽  
High Performance ◽  
Future Prospects ◽  
High Quality ◽  
Printing Technology ◽  
Wide Range ◽  
The Moment ◽  
Manufacturing Technologies

In recent years, additive manufacturing has gained importance in a wide range of research applications such as medicine, biotechnology, engineering, etc. It has become one of the most innovative and high-performance manufacturing technologies of the moment. This review aims to show and discuss the characteristics of different existing additive manufacturing technologies for the construction of micromixers, which are devices used to mix two or more fluids at microscale. The present manuscript discusses all the choices to be made throughout the printing life cycle of a micromixer in order to achieve a high-quality microdevice. Resolution, precision, materials, and price, amongst other relevant characteristics, are discussed and reviewed in detail for each printing technology. Key information, suggestions, and future prospects are provided for manufacturing of micromixing machines based on the results from this review.

scholarly journals Determination of the Anatomical Features of the Temporal Bones at the Stage of Planning Extraoral Implantation in Ectoprosthetics of the Auricle

2021 ◽  
pp. 12-24
Author(s):  
D. A. Lezhnev ◽  
S. D. Arutyunov ◽  
E. Yu. Lazarenko ◽  
M. V. Magomedbekova
Keyword(s):  
Surgical Intervention ◽  
Maxillofacial Surgery ◽  
Present Stage ◽  
High Quality ◽  
Anatomical Features ◽  
Prosthetic Reconstruction ◽  
Intervention Planning ◽  
Temporal Bones ◽  
Fixation System

At the present stage of the development of maxillofacial surgery, the treatment of patients with defects in the auricles is one of the most difficult. Defects of the auricles, characterized by its absence, are divided according to the etiological factor into two main groups: congenital (microtia) and acquired (as a result of injuries of various origins). Microtia (including anotia the complete absence of the auricle) is a malformation of the auricle and the external auditory canal caused by anomalies of the first and second zygomatic arch and the first sulcus.Congenital malformations of the auricle occur in 1 out of 700–15 000 newborns and more often have a right-sided localization. In 15 % of cases, they are hereditary, and in 85 % they are sporadic, in boys they are detected on average 2–2,5 times more often than in girls.Currently, much attention is paid to the prosthetic reconstruction of the auricle with fixation by means of cranial intraosseous implants. At the same time precise diagnostics of the state of the temporal bones in the area of the proposed implantation and the correct positioning of the intraosseous supports are the basis of an effective fixation system. Among the methods of radiological diagnostics used for planning ectoprosthetics of the auricles, radiography has long been preferred. However, the introduction of computed tomography into clinical practice has provided a high-quality assessment of the temporal bones state, which has increased the efficiency of surgical intervention planning and treatment.

Wood anatomy of historic French violin bows made of Pernambuco wood

IAWA Journal ◽  
2020 ◽  
Vol 41 (3) ◽  
pp. 320-332
Author(s):  
Tahysa Mota Macedo ◽  
Cecília Gonçalves Costa ◽  
Haroldo Cavalcante de Lima ◽  
Claudia Franca Barros
Keyword(s):  
Wood Anatomy ◽  
Principal Component ◽  
Rio Grande ◽  
Lower Percentage ◽  
High Quality ◽  
String Instruments ◽  
Axial Parenchyma ◽  
Anatomical Features ◽  
19Th And 20Th Century ◽  
Brazilian Northeast

Abstract Paubrasilia echinata is recognized as the best wood in the manufacture of high-quality bows for string instruments. The wood anatomy of five historic French violin bows of the 19th and 20th century made of Pernambuco wood were investigated in order to reveal the wood anatomic features of these historical bows, to determine which P. echinata morphotype (arruda, café or laranja) was used in their manufacture and to identify the state of origin of the wood. Five bow samples were compared to 33 P. echinata specimens from the Brazilian states of Rio de Janeiro, Bahia, Paraíba and Rio Grande do Norte. The wood anatomical features were compared by means of principal component analysis, which revealed the type of axial parenchyma and percentage of tissue to be the most important to sort specimens. The best wood anatomical features previously described for high-quality bows were corroborated here and the bows in general showed similar wood anatomical features. Based on wood anatomy we found that the violin bows were most similar to the samples from the arruda morphotype derived from the States of Paraíba and Rio Grande do Norte by presenting scanty, unilateral and vasicentric axial parenchyma without confluences forming bands, higher percentage of fibres and lower percentage of axial parenchyma. We can therefore suggest that the historical French violin bows studied here were all made of the arruda morphotype from the Brazilian Northeast region helping explain the preference of the French explorers for this region.

Human zinc fingers as building blocks in the construction of artificial transcription factors

2003 ◽  
Vol 21 (3) ◽  
pp. 275-280 ◽  
Author(s):  
Kwang-Hee Bae ◽  
Young Do Kwon ◽  
Hyun-Chul Shin ◽  
Moon-Sun Hwang ◽  
Eun-Hyun Ryu ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Zinc Fingers ◽  
Building Blocks ◽  
Artificial Transcription Factors

scholarly journals Development, progress and future prospects in cryobiotechnology of Lilium spp.

Plant Methods ◽  
2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Jing-Wei Li ◽  
Xiao-Chen Zhang ◽  
Min-Rui Wang ◽  
Wen-Lu Bi ◽  
M. Faisal ◽  
...  
Keyword(s):  
Cold Stress ◽  
Genetic Resources ◽  
Easy Access ◽  
Future Prospects ◽  
Breeding Programs ◽  
Future Studies ◽  
Comprehensive Information ◽  
Long Term Preservation ◽  
Made In

Abstract Lilium is one of the most popular flower crops worldwide, and some species are also used as vegetables and medicines. The availability of and easy access to diverse Lilium genetic resources are essential for plant genetic improvements. Cryopreservation is currently considered as an ideal means for the long-term preservation of plant germplasm. Over the last two decades, great efforts have been exerted in studies of Lilium cryopreservation and progress has been made in the successful cryopreservation of pollen, seeds and shoot tips in Lilium. Genes that exist in Lilium, including those that regulate flower shape, color and size, and that are resistant to cold stress and diseases caused by fungi and viruses, provide a rich source of valuable genetic resources for breeding programs to create novel cultivars required by the global floriculture and ornamental markets. Successful cryopreservation of Lilium spp. is a way to preserve these valuable genes. The present study provides updated and comprehensive information about the development of techniques that have advanced Lilium cryopreservation. Further ideas are proposed to better direct future studies on Lilium cryobiotechnology.

scholarly journals The natural history of the WRKY–GCM1 zinc fingers and the relationship between transcription factors and transposons

2006 ◽  
Vol 34 (22) ◽  
pp. 6505-6520 ◽  
Author(s):  
M. Madan Babu ◽  
Lakshminarayan M. Iyer ◽  
S. Balaji ◽  
L. Aravind
Keyword(s):  
Transcription Factors ◽  
Natural History ◽  
Zinc Fingers ◽  
History Of ◽  
The Relationship

scholarly journals Defining the human C2H2 zinc finger degrome targeted by thalidomide analogs through CRBN

Science ◽  
2018 ◽  
Vol 362 (6414) ◽  
pp. eaat0572 ◽  
Author(s):  
Quinlan L. Sievers ◽  
Georg Petzold ◽  
Richard D. Bunker ◽  
Aline Renneville ◽  
Mikołaj Słabicki ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Small Molecules ◽  
Zinc Finger ◽  
Ubiquitin Ligase ◽  
Biochemical Analysis ◽  
Zinc Fingers ◽  
Functional Characterization ◽  
C2h2 Zinc Finger

The small molecules thalidomide, lenalidomide, and pomalidomide induce the ubiquitination and proteasomal degradation of the transcription factors Ikaros (IKZF1) and Aiolos (IKZF3) by recruiting a Cys2-His2 (C2H2) zinc finger domain to Cereblon (CRBN), the substrate receptor of the CRL4CRBN E3 ubiquitin ligase. We screened the human C2H2 zinc finger proteome for degradation in the presence of thalidomide analogs, identifying 11 zinc finger degrons. Structural and functional characterization of the C2H2 zinc finger degrons demonstrates how diverse zinc finger domains bind the permissive drug-CRBN interface. Computational zinc finger docking and biochemical analysis predict that more than 150 zinc fingers bind the drug-CRBN complex in vitro, and we show that selective zinc finger degradation can be achieved through compound modifications. Our results provide a rationale for therapeutically targeting transcription factors that were previously considered undruggable.

Sign in / Sign up

Export Citation Format

Share Document