scholarly journals Development and Multicenter Evaluation of the N Latex CDT Direct Immunonephelometric Assay for Serum Carbohydrate-Deficient Transferrin

2007 ◽  
Vol 53 (6) ◽  
pp. 1115-1121 ◽  
Author(s):  
Joris R Delanghe ◽  
Anders Helander ◽  
Jos PM Wielders ◽  
J Maurits Pekelharing ◽  
Heinz J Roth ◽  
...  
Keyword(s):  
Alcohol Abuse ◽  
Genetic Variants ◽  
Reference Method ◽  
Sample Treatment ◽  
Serum Samples ◽  
Column Separation ◽  
Direct Immunoassay ◽  
Carbohydrate Deficient Transferrin ◽  
Healthy Control ◽  
Immunonephelometric Assay

Abstract Background: Carbohydrate-deficient transferrin (CDT) is a promising biomarker of alcohol abuse. We describe the development and multicenter evaluation of N Latex CDT (Dade Behring), an automated, particle-enhanced, homogeneous immunonephelometric assay for directly determining CDT. Methods: N Latex CDT uses a monoclonal antibody that recognizes the structure of transferrin glycoforms lacking 1 or 2 complete N-glycans [i.e., disialo-, monosialo-, and asialotransferrins (CDT glycoforms)] in combination with a simultaneous assay for total transferrin. The Dade Behring BN II™ and BN ProSpec® systems automatically calculate the CDT value as a percentage of total transferrin (%CDT). No preanalytical sample treatment is used. Results: Total imprecision values for serum pools containing 1.8%–8.7% CDT were 3.4%–10.4% (mean, 6.8%). The mean (SD) %CDT for 561 serum samples from healthy control individuals was 1.76% (0.27%; range, 1.01%–2.85%). No marked sex or age differences were noted. The 97.5th percentile was at 2.35%. Transferrin genetic variants did not interfere with measurements. High transferrin concentrations did not falsely increase %CDT values, but increased %CDT values were noted for some samples with transferrin concentrations <1.1 g/L. N Latex CDT results correlated with those of a commercial CDT immunoassay involving column separation (r2 = 0.862) and an HPLC candidate reference method (r2 = 0.978). Conclusion: N Latex CDT is the first direct immunoassay for quantifying %CDT in serum. The specificity of N Latex CDT for identifying alcohol abuse may be higher than for immunoassays that use column separation, because transferrin genetic variants do not interfere with measurements.

Evaluation of carbohydrate-deficient transferrin measurements on the V8 capillary electrophoresis system and comparison with the IFCC approved HPLC reference method and N-Latex immunonephelometric assay

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Leo H.J. Jacobs ◽  
Riekie M. te Stroet ◽  
Ayse Y. Demir
Keyword(s):  
High Performance ◽  
Clinical Chemistry ◽  
Method Comparison ◽  
Reference Method ◽  
Roc Curves ◽  
Serum Samples ◽  
Roc Curve Analysis ◽  
Chronic Alcohol Abuse ◽  
Carbohydrate Deficient Transferrin ◽  
Immunonephelometric Assay

AbstractObjectivesCarbohydrate-deficient transferrin (CDT) measurements are commonly used for the identification and follow-up of individuals suspected of chronic alcohol abuse. This study describes the analytical characteristics of the CDT assay on the Helena Biosciences V8 electrophoresis analyzer and compares its diagnostic performance to the International Federation of Clinical Chemistry and Laboratory Medicine approved high performance liquid chromatography (HPLC) method and the N-Latex CDT immunonephelometric assay.MethodsThe analytical performance of the V8 assay, including the linearity and the imprecision, was studied at two separate locations. Method comparison analysis was performed by studying the correlation, bias and agreement between the V8, HPLC and the N-Latex assays in 231 patient samples.ResultsThe total imprecision ranged between 5.1 and 24.3% and was ≤13.1% for samples with concentrations above the clinical cut-off value (≥1.62%). The method comparisons revealed excellent correlations with r2≥0.97 for all comparisons. Measurements on the V8 showed a bias of −0.83 (−22.24%) and −0.40 (−12.26%) with the HPLC and N-Latex assays, respectively. The assays showed excellent agreements (Kappa scores ≥ 0.8) in classifying subjects with elevated CDT values. Receiver operating characteristic (ROC)-curve analysis, using the HPLC classification as reference, revealed areas under the ROC-curves of 0.981 (95% CI, 0.97–0.99) and 0.996 (0.99–1.00) for the N-Latex and V8 assays, respectively.ConclusionsCDT measurements on the V8 assay are highly correlated with both the HPLC and the N-Latex assay and show excellent agreement in classifying subjects with elevated CDT values. Overall, the V8 CDT analysis is a robust, reliable and effective method to measure CDT concentrations in serum samples.

Carbohydrate-deficient transferrin and false-positive results for alcohol abuse in primary biliary cirrhosis: differential diagnosis by detection of mitochondrial autoantibodies

1995 ◽  
Vol 41 (6) ◽  
pp. 858-861 ◽  
Author(s):  
P Bean ◽  
M S Sutphin ◽  
Y Liu ◽  
R Anton ◽  
T B Reynolds ◽  
...  
Keyword(s):  
Primary Biliary Cirrhosis ◽  
Alcohol Abuse ◽  
Test Performance ◽  
Roc Analysis ◽  
Biliary Cirrhosis ◽  
Antibody Testing ◽  
Serum Samples ◽  
Optimal Test ◽  
Carbohydrate Deficient Transferrin ◽  
Mitochondrial Antibody

Abstract Primary biliary cirrhosis (PBC) is one of the few nonalcohol-induced liver pathologies that causes false positives in assays of carbohydrate-deficient transferrin (CDT) for diagnosing alcohol abuse. CDT was quantified by isoelectric focusing-immunoblotting-laser densitometry (IEF-IB-LD) analysis of serum from 117 women: 57 PBC patients, 20 alcohol abusers, and 40 healthy donors. Only 5% (3 of 57) of PBC patients were positive at the densitometric cutoff value chosen (> 90% specificity). Serum samples from 15 PBC patients were further evaluated by IEF-IB-LD and CDTect chromatography-RIA. Receiver-operating characteristic (ROC) analysis showed that IEF-IB-LD better discriminated between PBC and alcohol abuse than CDTect did. By ROC analysis, mitochondrial autoantibodies to pyruvate dehydrogenase antigen M2 detected by enzyme immunoassay yielded optimal test performance for diagnosing PBC. Of six patients falsely positive for CDT by CDTect, five (83%) tested M2-positive. Thus, abnormal CDT results should be further evaluated by mitochondrial antibody testing in patients with findings compatible with PBC.

scholarly journals A proof-of-concept analysis of carbohydrate-deficient transferrin by imaged capillary isoelectric focusing and in-capillary immunodetection

BioTechniques ◽  
2020 ◽  
Vol 68 (2) ◽  
pp. 85-90 ◽  
Author(s):  
Jiaqi Wu ◽  
Charles H Haitjema ◽  
Christopher D Heger ◽  
Annegret Boge
Keyword(s):  
Alcohol Abuse ◽  
Isoelectric Focusing ◽  
Capillary Isoelectric Focusing ◽  
Chronic Alcohol ◽  
Proof Of Concept ◽  
Serum Samples ◽  
Chronic Alcohol Abuse ◽  
Isoelectric Points ◽  
Carbohydrate Deficient Transferrin ◽  
Expected Values

Carbohydrate-deficient transferrin (CDT) is a reliable biomarker for chronic alcohol abuse. We developed a method for CDT analysis by capillary isoelectric focusing, followed by immunodetection directly in the capillary, in an automated fashion and on a single platform (Peggy Sue™; ProteinSimple, CA, USA). Transferrin glycoforms in serum samples, including disialo-transferrin, were separated and their apparent isoelectric points and relative percentages were determined. The relative CDT values (percent of total transferrin) matched expected values for both healthy and alcoholic samples. Because the method leveraged the sensitivity of an immunoassay, CDT was measured when serum samples were diluted up to 1200-fold, reducing the volume of serum required. Finally, the process is fully automated, with up to 96 samples analyzed per batch.

A Simple Method for Carbohydrate-Deficient Transferrin Measurement in Patients with Alcohol Abuse and Hepato-Gastrointestinal Diseases

1998 ◽  
Vol 35 (2) ◽  
pp. 268-273 ◽  
Author(s):  
F Cotton ◽  
M Adler ◽  
J Dumon ◽  
J-M Boeynaems ◽  
B Gulbis
Keyword(s):  
Alcohol Abuse ◽  
Isoelectric Focusing ◽  
Mean Corpuscular Volume ◽  
Gastrointestinal Diseases ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Corpuscular Volume ◽  
Simple Method ◽  
Carbohydrate Deficient Transferrin ◽  
Healthy Control

Carbohydrate-deficient transferrin (CDT) is known to be increased in alcohol abuse. Several methods were developed for its measurement (e.g. isoelectric focusing with Western blotting or immunofixation, anion-exchange chromatography followed by immunoassays). We describe a greatly simplified isoelectric focusing technique which does not require immunofixation. CDT results obtained with this method were compared to other biological markers of alcohol abuse, i.e. mean corpuscular volume (MCV), aspartate aminotransferase (ASAT) and γ-glutamyltransferase (GGT), in 55 patients distributed in three groups (i.e. healthy control subjects, control patients suffering from various hepato-gastrointestinal diseases and alcohol abusing patients). Sensitivity and specificity were 33 89%, 61–57%, 89–49% and 83–100% for MCV, ASAT, GGT and CDT, respectively. We conclude that our method is highly suitable for routine clinical use.

scholarly journals Transferrina-Carboidrato carente (CDT): Updating sul marcatore biochimico più studiato ed utilizzato in ambito alcologico

2014 ◽  
Vol 3 (1) ◽  
Author(s):  
V. Bianchi
Keyword(s):  
Alcohol Abuse ◽  
Current Issue ◽  
Alcohol Intoxication ◽  
Reference Method ◽  
Effective Strategies ◽  
Carbohydrate Deficient Transferrin ◽  
Standardization Process ◽  
Diagnostic Sensitivity And Specificity ◽  
Physical Consequences ◽  
Guide Lines

Alcohol use and abuse is a important current issue, in addiction it is a social problem leading to isolation, physical consequences and several pathologies. Alcohol intoxication is correlated to accidents, injuries and violence. Developing effective strategies to prevent, diagnose and cure alcohol abuse is a priority as well as to have sure evidences. Carbohydrate-deficient transferrin (CDT) is the most specific biomarker of alcohol abuse. In this review biochemistry, metabolism and definition are showed. Pre-analytical, analytical and post-analytical phases are discussed In this review several CDT applications in hospital , in rehabilitation and in forensic setting are presented. HPLC, CZE and immunoassay methods are presented. The need of standardization of the measurement of CDT is explained, because defining main target analyte, reference method and materials are important to have the same result with different methods. Some criticisms on the standardization process are showed. Diagnostic sensitivity and specificity are discussed and how correctly calculated the cut off and a correct interpretation of data are reported. Guide-lines by SIBioC-GITF are reminded.

scholarly journals COT-04 Circulating biomarker for glioblastoma and primary central nervous system lymphoma -Next Generation Sequencing of small noncoding RNA-

2020 ◽  
Vol 2 (Supplement_3) ◽  
pp. ii21-ii21
Author(s):  
Shumpei Onishi ◽  
Fumiyuki Yamasaki ◽  
Motoki Takano ◽  
Ushio Yonezawa ◽  
Kazuhiko Sugiyama ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Noncoding Rna ◽  
Central Nervous System Lymphoma ◽  
Serum Samples ◽  
Small Noncoding Rna ◽  
Non Invasive ◽  
Diagnostic Models ◽  
Healthy Control ◽  
Generation Sequencing

Abstract Objective: Glioblastoma (GBM) and Primary Central Nervous System Lymphoma (PCNSL) are common intracranial malignant tumors. They sometimes present similar radiological findings and diagnoses could be difficult without surgical biopsy. For improving the current management, development of non-invasive biomarkers are desired. In this study, we explored the differently expressed circulating small noncoding RNA (sncRNA) in serum for specific diagnostic tool of GBM and PCNSL. Material & Methods: Serum samples were obtained from three groups: 1) GBM patients (N=26), 2) PCNSL patients (N=14) 3) healthy control (N=114). The total small RNAs were extracted from serum. The whole expression profiles of serum sncRNAs were measured using Next-Generation Sequencing System. We analyzed serum levels of sncRNAs (15–55 nt) in each serum samples. The difference of sncRNAs expression profile among three groups were compared. Data analysis was performed by logistic regression analysis followed by leave-one-out cross-validation (LOOCV). The accuracy of diagnostic models of sncRNAs combination were evaluated by receiver operating characteristic (ROC) analysis. Results: We created the combination models using three sncRNA in each models based on the logistic regression analysis. The model 1 (based on sncRNA-X1, X2 and X3) enabled to differentiate GBM patients form healthy control with a sensitivity of 92.3% and a specificity of 99.2% (AUC: 0.993). The model 2 (based on sncRNA-Y1, Y2 and Y3) enabled to differentiate PCNSL patients form healthy control with a sensitivity of 100% and a specificity of 93.9% (AUC: 0.984). The model 3 (based on sncRNA-Z1, Z2 and Z3) enabled to differentiate GBM patients form PCNSL patients with a sensitivity of 92.3% and a specificity of 78.6% (AUC: 0.920). Conclusion: We found three diagnostic models of serum sncRNAs as non-invasive biomarkers potentially useful for detection of GBM and PCNSL from healthy control, and for differentiation GBM from PCNSL.

scholarly journals Carbohydrate deficient transferrin: a marker for alcohol abuse.

BMJ ◽  
1989 ◽  
Vol 299 (6696) ◽  
pp. 427-431 ◽  
Author(s):  
A. Kapur ◽  
G. Wild ◽  
A. Milford-Ward ◽  
D. R. Triger
Keyword(s):  
Alcohol Abuse ◽  
Carbohydrate Deficient Transferrin

Optimization of Automated Sample Preparation for Vitamin D Determination on a Biomek i7 Workstation

2021 ◽  
pp. 247263032110302
Author(s):  
Anna Bach ◽  
Heidi Fleischer ◽  
Bhagya Wijayawardena ◽  
Kerstin Thurow
Keyword(s):  
Vitamin D ◽  
Sample Preparation ◽  
Reference Method ◽  
Positive Pressure ◽  
Additional Risk Factor ◽  
Serum Samples ◽  
Recovery Rates ◽  
Preparation Methods ◽  
Sample Throughput ◽  
Automated Sample Preparation

Vitamin D belongs to the fat-soluble vitamins and is an integral part of bone metabolism. In the human body, a decreased vitamin D level can be an additional risk factor for diseases like cancer, diabetes, and mental diseases. As a result, an enormous increase in the demand for vitamin D testing has been observed in recent years, increasing the demand for powerful methods for vitamin D determination at the same time. Automation is the key factor in increasing sample throughput. This study compares three fully automated sample preparation methods for the determination of 25(OH)D2 and 25(OH)D3 in plasma and serum samples. Starting from a semiautomated reference method, the method is tested manually and subsequently fully automated on the Biomek i7 Workstation by integrating a centrifuge and a positive pressure extractor into the workstation. Alternatively, the centrifugation for the separation of protein aggregates and supernatant is replaced by a filter plate. Finally, the sample throughput is further increased by using phospholipid removal cartridges. The results show that phospholipid removal significantly increases the recovery rates in liquid chromatography–mass spectrometry. With the phospholipid removal cartridges, recovery rates of 97.36% for 25(OH)D2 and 102.5% for 25(OH)D3 were achieved, whereas with the automated classic automated preparation method, the recovery rates were 83.31% for 25(OH)D2 and 86.54% for 25(OH)D3. In addition to the technical evaluation, the different methods were also examined with regard to their economic efficiency. Finally, the qualitative and quantitative performance of the developed methods is benchmarked with a selected semiautomatic reference method.

scholarly journals Studying the Role of C-Reactive Protein in Patients with Sleeve Gastrectomy in Baghdad

2021 ◽  
Vol 3 (1) ◽  
pp. 55-60
Author(s):  
Mohammed Abbas Fadil ◽  
Raya Ezat Maroof ◽  
Moayed Abbas Fadil
Keyword(s):  
Sleeve Gastrectomy ◽  
Healthy Person ◽  
Control Group ◽  
C Reactive Protein ◽  
Serum Samples ◽  
Reactive Protein ◽  
Medical City ◽  
Healthy Control ◽  
And Control

Obesity and severe obesity are increasing serious health problems with an epidemic percentage in most countries. In Sleeve gastrectomy, a part of the stomach structure is removed, limiting its capacity by about two to three. A total of thirty blood samples were collected from patients with obesity and the result was compared with healthy person throughout the time from November 2019 to September 2020. Before sleeve gastrectomy and after more than 6 months of sleeve surgery, the sample was collected from the Medical City/Baghdad Teaching Hospital, the withdrawal was again taken at home to have pre and post sleeve gastrectomy, Patient age ranged between [20-46] years for obese patients and healthy control. Then the serum samples were obtained from patients and control group to screen for C-reactive protein by agglutination method. The result of the present study found that the positivity of CRP in pre-operation is higher than that of post-operative with high significance [P<0.005].

Sign in / Sign up

Export Citation Format

Share Document