scholarly journals Buprenorphine and Norbuprenorphine in Hair of Pregnant Women and Their Infants after Controlled Buprenorphine Administration

2007 ◽  
Vol 53 (12) ◽  
pp. 2136-2143 ◽  
Author(s):  
Robert S Goodwin ◽  
Diana G Wilkins ◽  
Olga Averin ◽  
Robin E Choo ◽  
Jennifer R Schroeder ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Opioid Dependence ◽  
Drug Disposition ◽  
Methylene Chloride ◽  
Positive Association ◽  
Significant Positive Association ◽  
Chromatography Tandem Mass Spectrometry ◽  
Monitoring Drug ◽  
Liquid Chromatography Tandem Mass ◽  
Buprenorphine Treatment

Abstract Background: Buprenorphine is under investigation as a pharmacotherapeutic agent for treating opioid dependence in pregnant women. We hypothesized that there would be a relationship between the cumulative maternal dose of buprenorphine during pregnancy and the concentration of buprenorphine and norbuprenorphine in maternal and infant hair. Methods: This study examined buprenorphine and norbuprenorphine concentrations in hair obtained from 9 buprenorphine-maintained pregnant women and 4 of their infants. Specimens were analyzed by liquid chromatography-tandem mass spectrometry with limits of quantification of 3.0 pg/mg. All maternal hair specimens were washed with methylene chloride before analysis, and when sufficient amounts of maternal hair were available, specimens also were analyzed without washing. Infant hair specimens were not washed. Results: Buprenorphine concentrations were significantly greater in unwashed hair than washed hair (P = 0.031). Norbuprenorphine concentrations were significantly greater than buprenorphine concentrations in both maternal (P = 0.0097) and infant hair (P = 0.0033). There were statistically significant associations between the cumulative maternal dose of buprenorphine administered and the concentrations of buprenorphine (washed, P <0.0001; unwashed, P = 0.0004), norbuprenorphine (washed, P <0.0001; unwashed, P = 0.0005), and buprenorphine plus norbuprenorphine (washed, P <0.0001; unwashed, P = 0.0005) for both washed and unwashed maternal hair specimens. There was a significant positive association between concentrations of buprenorphine and norbuprenorphine in maternal hair (washed, P <0.0001; unwashed, P = 0.0003), a trend for this association in infant hair (P = 0.08), and an association between buprenorphine concentrations in maternal unwashed hair and infant hair (P = 0.0002). The buprenorphine:norbuprenorphine ratio increased in distal segments. Conclusion: Buprenorphine treatment during gestation provides an opportunity for monitoring drug disposition in maternal and fetal tissues under controlled conditions.

scholarly journals Altered Bioavailability of Nitric Oxide and L-Arginine Is a Key Determinant of Endothelial Dysfunction in Preeclampsia

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Worlanyo Tashie ◽  
Linda Ahenkorah Fondjo ◽  
William K. B. A. Owiredu ◽  
Richard K. D. Ephraim ◽  
Listowell Asare ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Blood Pressure ◽  
Endothelial Dysfunction ◽  
Pregnant Women ◽  
Positive Association ◽  
Neonatal Morbidity ◽  
Sub Saharan Africa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Low Birth Weight Infants ◽  
Significant Positive Association

Background. Preeclampsia is a major cause of maternal and neonatal morbidity and mortality in sub-Saharan Africa. Evidence indicates that endothelial dysfunction is central to the pathogenesis of preeclampsia. This study assessed the level of the components of the arginine-nitric oxide pathway to evaluate endothelial dysfunction in normotensive pregnancies and pregnancies complicated with preeclampsia. Methods. This case-control study was conducted among pregnant women who visited Comboni Hospital from January 2017 to May 2018. A total of 180 pregnant women comprising 88 preeclamptic women (PE) and 92 healthy normotensive pregnant women (NP) were recruited. Sociodemographic, clinical, and obstetric data were obtained using validated questionnaires. Blood pressure and anthropometrics were measured, and blood samples were collected for the estimation of nitric oxide (NO∙), L-arginine, asymmetric dimethylarginine (ADMA), and 3-nitrotyrosine using an enzyme-linked immunosorbent assay technique. Results. The mean NO∙ ( p = 0.010 ) and L-arginine/ADMA ratio ( p < 0.0001 ) was significantly lower in PE compared to NP while mean L-arginine ( p = 0.034 ), ADMA ( p < 0.0001 ), and 3-nitrotyrosine ( p < 0.0001 ) were significantly higher in PE than NP. ADMA showed a significant positive association with systolic blood pressure ( β = 0.454 , p = 0.036 ) in severe PE. Women with PE had significant intrauterine growth restriction ( p < 0.0001 ) and low birth weight infants ( p < 0.0001 ) when compared to NP. Conclusion. Preeclampsia is associated with reduced NO∙ bioavailability, L-arginine/ADMA ratio, and elevated levels of ADMA and 3-nitrotyrosine. Measurements of the levels of these parameters can help in the early prediction of endothelial dysfunction in preeclampsia. Exogenous therapeutic supplementation with L-arginine during pregnancy to increase the L-arginine/ADMA ratio should be considered to improve endothelial function in preeclampsia and pregnant women at risk of developing preeclampsia.

scholarly journals Plasma Free Amino Acid and Carnitine Levels in Pregnant Women with Hydatidiform Mole: A Case-Controlled Study

2021 ◽  
Author(s):  
Hacer Uyanıkoğlu ◽  
İsmail Koyuncu ◽  
Sibel sak ◽  
adnan incebiyik ◽  
nese gul hilali ◽  
...  
Keyword(s):  
Amino Acid ◽  
Pregnant Women ◽  
Free Amino Acid ◽  
Free Amino ◽  
Venous Blood ◽  
Hydatidiform Mole ◽  
Controlled Study ◽  
Control Group ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Objective: To compare plasma free amino acid (FAA) and carnitine levels in pregnant women with and without hydatidiform mole (HM). Methods: This prospective study was conducted in patients admitted to Harran University Medical Faculty Hospital Obstetrics Clinic between January and November 2018. Twenty-three pregnant women with HM, and 24 healthy pregnant women as controls were enrolled in the study. The venous blood samples were collected, and, FAA and carnitine concentrations were measured in plasma using liquid chromatography/tandem mass spectrometry (LC-MS/MS). Results: The levels of alanine, arginine, and valine from the 14 amino acids examined were significantly lower in the HM group than in the healthy group (475.46 ± 103.55 µmol/L vs 556.99 ± 105.16 µmol/L, p = 0.019; 428.87 ± 138.91 µmol/L vs 547.11 ± 140.20 µmol/L, p = 0.009; and 290.96 ± 97.10 µmol/L vs 358.61 ± 75.40 µmol/L, p = 0.03; respectively). In addition, several carnitines, C8DC, C16:1, and C18, of the 27 carnitines examined were significantly higher in the HM group than in the control group (0.07 ± 0.06 µmol/L vs 0.03 ± 0.01 µmol/L, p = 0.021; 0.18 ± 0.09 µmol/L vs 0.10 ± 0.06 µmol/L, p = 0.03; and 0.07 ± 0.02 µmol/L vs 0.03 ± 0.01 µmol/L, p = 0.021; respectively). Conclusion: This study demonstrated that a decrease in some plasma FAAs and an increase in some plasma carnitine levels might be effective in the pathogenesis of HM.

Perceived Overprotection and Its Association With Quality of Life in Dementia

GeroPsych ◽  
2019 ◽  
Vol 32 (3) ◽  
pp. 125-134
Author(s):  
Mechthild Niemann-Mirmehdi ◽  
Andreas Häusler ◽  
Paul Gellert ◽  
Johanna Nordheim
Keyword(s):  
Quality Of Life ◽  
Patient Autonomy ◽  
Positive Association ◽  
Well Being ◽  
Negative Association ◽  
Significant Positive Association ◽  
Cross Sectional ◽  
People With Dementia ◽  
Mental Well Being

Abstract. To date, few studies have focused on perceived overprotection from the perspective of people with dementia (PwD). In the present examination, the association of perceived overprotection in PwD is examined as an autonomy-restricting factor and thus negative for their mental well-being. Cross-sectional data from the prospective DYADEM study of 82 patient/partner dyads (mean age = 74.26) were used to investigate the association between overprotection, perceived stress, depression, and quality of life (QoL). The analyses show that an overprotective contact style with PwD has a significant positive association with stress and depression, and has a negative association with QoL. The results emphasize the importance of avoiding an overprotective care style and supporting patient autonomy.

Determinants of Buprenorphine Treatment for Opioid Dependence

2013 ◽  
Author(s):  
Sean M. Murphy ◽  
Paul A. Fishman ◽  
Sterling M. McPherson ◽  
Dennis G. Dyck ◽  
John M. Roll
Keyword(s):  
Opioid Dependence ◽  
Buprenorphine Treatment
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