scholarly journals Determination of progesterone compounds in the crude methanol extract of benalu duku leaves

2019 ◽  
Vol 12 (3) ◽  
pp. 358-366
Author(s):  
Lazuardi Mochamad ◽  
Bambang Hermanto ◽  
E. P. Hestianah
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Methanol Extract ◽  
Array Detector ◽  
Ionization Mass ◽  
Analytical Column ◽  
Photodiode Array Detector ◽  
Ft Ir ◽  
The Fourier Transform ◽  
Crude Methanol Extract

Background and Aim: Dendrophthoe pentandra L. Miq (benalu duku) is a parasitic herb that commonly grows on the host plant Lansium domesticum. Researchers have found that the plant contains anticancer compounds and may contain phytoandrogens, including progesterone-like compounds, in its crude methanol extract. The objective of the current study was to investigate the compound of phyto progesterone in benalu duku leaves after extracted by methanol and prepared using an analytical column of high-performance liquid chromatography (HPLC). Materials and Methods: About 400 g of benalu duku leaves were pulverized, and their compounds were isolated by the isocratic method using an RP-18 analytical column (5 μm) with a mobile phase of 70:30 (methanol: water) in a photodiode array detector adjusted to 254 nm. The phyto progesterone compound was identified at a retention time of approximately 6.01 min. Results: By LC-electrospray ionization mass spectrometry focusing on molecular fractions, the fingerprint area of the Fourier transform-infrared spectroscopy (FT-IR, cm−1) and Hnuclear magnetic resonance (NMR) spectra indicated that the phyto progesterone product isolated was identical to the certified reference material of pure progesterone, particularly the specific functional groups in the FT-IR spectrum at wavenumbers of 1317.43 cm−1 and 1386.86 cm−1 and in the proton HNMR spectrum at carbon 21 of progesterone (p<0.05). Conclusion: Each 49.888 μg/mL of crude benalu duku leaf extract dissolved in the mobile phase contained 28.515±0.713 μg/mL phyto progesterone.

scholarly journals DETERMINATION OF LUTEOLIN FROM EXTRACTIONS OF Helicteres hirsuta BY HPLC

2019 ◽  
Vol 128 (1B) ◽  
pp. 43
Author(s):  
Lê Trung Hiếu ◽  
Lê Lâm Sơn ◽  
Nguyễn Minh Nhung ◽  
Hồ Xuân Anh Vũ ◽  
Trần Thị Văn Thi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Array Detector ◽  
Plant Parts ◽  
Aerial Parts ◽  
Photodiode Array Detector ◽  
Photodiode Array

<p>High performance liquid chromatography coupled with photodiode array detector (HPLC- DAD) has been reported to quantify isolated compounds. This work was designed, therefore, to develop an HPLC-DAD system to determine luteolin in extractive solutions from <em>Helicteres hirsuta</em>. Luteolin was analyzed on a RP- C18 column, using a mobile phase including: acetonitrile - 0.1% acid phosphoric (v/v), under the following conditions detecting wavelength: 347 nm; flow rate: 0.5 mL/min, and the volume of injecting sample: 10 μL. The HPLC system was carried out at ambient temperature. The method showed linearity for luteolin in the range to 0.02 from 1 mg/mL, and the recovery of luteolin was 94.07 ± 0.64. Contents of  luteolin in methanol extracts from the plant parts of <em>H. hirsuta</em> (including: branch, leaf, fruit and aerial parts) were determined with value of 49.06 ±0.46, 142.89 ±0.53, 56.61±0.62 and 91.15±0.42 µg/g, respectively.</p>

scholarly journals RP-HPLC-DAD-MSn Analysis and Butyrylcholinesterase Inhibitory Activity of Barbacenia blanchetii Extracts

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000
Author(s):  
Jósquia S Barbosa ◽  
Verônica M Almeida ◽  
Rosilene M Marçal ◽  
Alexsandro Branco
Keyword(s):  
High Performance ◽  
Methanol Extract ◽  
Array Detector ◽  
Ionization Mass ◽  
Diode Array Detector ◽  
Rp Hplc ◽  
Dependent Form ◽  
Ellman’S Method ◽  
First Time

High-performance liquid chromatography (HPLC) coupled to a diode-array detector (DAD) and electrospray ionization mass spectrometry (MS) was applied for the separation and identification of phenolic compounds in the n-hexane, ethyl acetate and methanol crude extract of Barbacenia blanchetii. The MS, MSn and UV data together with chemosystematic evidence allowed the structural characterization of five compounds: tricin, chrysoeriol, epi-gallocatechin, kaempferol 3- O-glucoside and caffeoylquinic acid. At the same time, these extracts were evaluated against butyrylcholinesterase using Ellman's method. All extracts inhibited BuChE in a concentration-dependent form; however, the methanol extract showed a better effect that the other extracts. These compounds have been identified previously in the Velloziaceae family, but for the first time as constituents of B. blanchetii.

scholarly journals Simultaneous Determination of Atorvastatin Calcium and Ramipril in Capsule Dosage Forms by High-Performance Liquid Chromatography and High-Performance Thin Layer Chromatography

2010 ◽  
Vol 93 (5) ◽  
pp. 1450-1457 ◽  
Author(s):  
Hiral J Panchal ◽  
Bhanubhai N Suhagia
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Dosage Forms ◽  
Array Detector ◽  
Atorvastatin Calcium ◽  
Photodiode Array Detector ◽  
Rf Values ◽  
Photodiode Array ◽  
Concentration Levels

Abstract Two simple and accurate methods to determine atorvastatin calcium and ramipril in capsule dosage forms were developed and validated using HPLC and HPTLC. The HPLC separation was achieved on a Phenomenex Luna C18 column (250 ⨯ 4.6 mm id, 5 µm) in the isocratic mode using 0.1% phosphoric acidacetonitrile (38 + 62, v/v), pH 3.5 ± 0.05, mobile phase at a flow rate of 1 mL/min. The retention times were 6.42 and 2.86 min for atorvastatin calcium and ramipril, respectively. Quantification was achieved with a photodiode array detector set at 210 nm over the concentration range of 0.5-5 µg/mL for each, with mean recoveries (at three concentration levels) of 100.06 ± 0.49% and 99.95 ± 0.63% RSD for atorvastatin calcium and ramipril, respectively. The HPTLC separation was achieved on silica gel 60 F254 HPTLC plates using methanolbenzeneglacial acetic acid (19.6 + 80.0 + 0.4, v/v/v) as the mobile phase. The Rf values were 0.40 and 0.20 for atorvastatin calcium and ramipril, respectively. Quantification was achieved with UV densitometry at 210 nm over the concentration range of 50500 ng/spot for each, with mean recoveries (at three concentration levels) of 99.98 ± 0.75% and 99.87 ± 0.83% RSD for atorvastatin calcium and ramipril, respectively. Both methods were validated according to International Conference on Harmonization guidelines and found to be simple, specific, accurate, precise, and robust. The mean assay percentages for atorvastatin calcium and ramipril were 99.90 and 99.55% for HPLC and 99.91 and 99.47% for HPTLC, respectively. The methods were successfully applied for the determination of atorvastatin calcium and ramipril in capsule dosage forms without any interference from common excipients.

Analgesic and Anti-Inflammatory Property of the Methanol Extract from Ligustrum morrisonense Leaves in Rodents

2011 ◽  
Vol 39 (02) ◽  
pp. 335-348 ◽  
Author(s):  
Chi-Rei Wu ◽  
Wen-Hsin Lin ◽  
Yung-Ta Lin ◽  
Chi-Luan Wen ◽  
Hui Ching ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Array Detector ◽  
Leaf Extracts ◽  
Ligustrum Lucidum ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Anti Inflammatory

Ligustrum morrisonense Kaneh and Sasaki (abbreviated as LM), an endemic Ligustrum plant in Taiwan, is similar to Ligustrum lucidum, which is usually used for curing hepatic and inflammatory disorders. The aim of this study was to evaluate the analgesic and anti-inflammatory properties of LM by chemical-induced algesia and carrageenan-induced inflammation in rodents. Its triterpenoid contents were measured by using high performance liquid chromatography-photodiode array detector. LM leaf extracts effectively inhibited writhing responses induced by 1% acetic acid and biphasic-licking responses caused by 1% formalin. LM leaf extract also reduced the edema induced by 1% carrageenan. Furthermore, LM leaf extract reduced the abdominal Evan's blue extravasations caused by lipopolysaccharide (LPS), serotonin, histamine and bradykinin. LM leaf extract has higher contents of amyrin and lupeol among six assayed triterpenoid compounds. In conclusion, LM is a potential analgesic and anti-inflammatory Ligustrum plant, and its anti-inflammatory effects are partially related to decreasing microvascular permeability via inflammatory mediators and inhibiting cyclooxygenase-2 activity.

scholarly journals Rapid and Simple Determination of Oxytetracycline in Chicken Products

1999 ◽  
Vol 82 (3) ◽  
pp. 770-772 ◽  
Author(s):  
Naoto Furusawa
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Limit Of Detection ◽  
Array Detector ◽  
Disodium Edta ◽  
Simple Method ◽  
Photodiode Array Detector ◽  
Coefficients Of Variation ◽  
Photodiode Array

Abstract A rapid and simple method for determining resid ual oxytetracycline (OTC) in chicken products(muscle, liver, and eggs) by high-performance liquid chromatography (HPLC) was developed. Samples were prepared by homogenization with acetonitrile-n-hexane (5 + 4, v/v) followed by centrifugation to minimize fat and protein contents. OTC in the acetonitrile layer was free from interfering compounds when examined by HPLC using a LiChrospher 100 RP-8 end-capped column, a mobile phase of acetonitrile–acetic acid–0.01 M disodium EDTA(28 + 2 + 70, v/v/v),and a photodiode array detector. Average recoveries from samples spiked with OTC (0.1, 0.2, and1.0 ppm) were &gt;88%, with coefficients of variation between 2.3 and 5.1 %. The limit of detection was 0.05 ppm.

Profiling and Quantification of the Key Phytochemicals from the Drumstick Tree (Moringa oleifera) and Dietary Supplements by UHPLC-PDA-MS

Planta Medica ◽  
2020 ◽  
Author(s):  
Omer I. Fantoukh ◽  
Yan-Hong Wang ◽  
Abidah Parveen ◽  
Mohammed F. Hawwal ◽  
Gadah A. Al-Hamoud ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Dietary Supplements ◽  
High Performance ◽  
Moringa Oleifera ◽  
Negative Ion ◽  
Array Detector ◽  
Ionization Mass ◽  
Fractionation Process ◽  
Chromatography Method ◽  
Drumstick Tree

Abstract Moringa oleifera is known as a drumstick tree and is cultivated in the subtropics and tropics. It exhibits antihypertensive and antidiabetic effects. An ultra-high-performance liquid chromatography method was developed for the determination of 9 phytochemicals in M. oleifera leaves and marketed products. The efficient separation was achieved within 7 min with a temperature of 45 °C by using a C-18 column as the stationary phase and water/acetonitrile with 0.05% formic acid as the mobile phase. The method was validated for linearity, repeatability, limits of detection, and limits of quantification. The limits of detections of phenolic compounds 1 – 9 were as low as 0.2 µg/mL. The photodiode array detector at 220 and 255 nm wavelengths was recruited for quantification. The key phytochemicals were detected in the range of 0.42 to 2.57 mg/100 mg sample weight in 13 dietary supplements. This study considers the quantitative analysis for lignans in M. oleifera for the first time. Isoquercitrin (5) and quercetin 3-O-(6-O-malonyl)-β−D-glucopyranoside (6) predominates the leaves of M. oleifera with inherent degradable nature detected for compound 6. Niazirin (2) was detected in amounts between 0.010 – 0.049 mg/100 mg while compound 1 was undetectable and potentially an artifact because of the fractionation process. The characterization and confirmation of components were achieved by liquid chromatography-electrospray ionization-mass spectrometry with extractive ion monitoring for the positive and negative ion modes. The developed and validated method is robust and rapid in the conclusive quantification of phytochemicals and authentication of the Moringa samples for quality assurance.

Development and validation of a fast and simple HPLC method for the simultaneous determination of aniline and its degradation products in wastewater

2016 ◽  
Vol 8 (30) ◽  
pp. 5949-5956 ◽  
Author(s):  
Soumia Boulahlib ◽  
Ali Boudina ◽  
Kahina Si-Ahmed ◽  
Yassine Bessekhouad ◽  
Mohamed Trari
Keyword(s):  
High Performance ◽  
Degradation Products ◽  
Reversed Phase ◽  
Hplc Method ◽  
Array Detector ◽  
Simple Method ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Development And Validation

In this study, a rapid and simple method based on reversed-phase high performance liquid chromatography (RP-HPLC) using a photodiode array detector (PDA) for the simultaneous analysis of five pollutants including aniline and its degradation products, para-aminophenol, meta-aminophenol, ortho-aminophenol and phenol, was developed.

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