scholarly journals Occurrence and antibiogram of Listeria species in raw pork, beef, and chicken meats marketed in Enugu State, Southeast Nigeria

2020 ◽  
Vol 13 (2) ◽  
pp. 317-325
Author(s):  
Onyinye Josephine Okorie-Kanu ◽  
Madubuike Umunna Anyanwu ◽  
Ekene Vivienne Ezenduka ◽  
Anthony Christian Mgbeahuruike ◽  
Christian Onwuchokwe Okorie-Kanu ◽  
...  
Keyword(s):  
Resistance Index ◽  
Multidrug Resistant ◽  
Chicken Meat ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Listeria Ivanovii ◽  
Enugu State ◽  
Meat Samples ◽  
Listeria Welshimeri ◽  
Pork Samples

Aim: This study was undertaken to isolate Listeria (L.) species from raw meats sold in markets in Enugu State, Southeast Nigeria, and to determine the antibacterial resistance profile. Materials and Methods: Twenty-five grams of beef (n=144), chicken meat (n=144), and pork (n=144) were collected randomly from supermarkets and general markets in Enugu State. Isolation of Listeria was done using half and full Fraser broths, and polymyxin acriflavine lithium chloride ceftazidime aesculin mannitol agar. Identification of isolates was done using an analytical profile index kit specific for Listeria. Confirmation of the genus Listeria was done by a polymerase chain reaction. The resistance of the isolates was determined using the disk diffusion method. Results: Listeria was isolated from 39/144 (27.1%) chicken meat, 19/144 (13.2%) pork, and 66/144 (45.8%) beef samples cultured. Listeria innocua was the predominant species in chicken meat (52.6%) and beef (81.8%) samples. Listeria grayi, Listeria welshimeri, and Listeria ivanovii were also isolated from the beef and chicken meat samples. More than 65% of the isolates were resistant to penicillin, rifampicin, ciprofloxacin, sulfamethoxazole-trimethoprim, and cephalothin. All the isolates from beef and pork samples and 23 (92%) from chicken meat samples, were resistant to ≥3 classes of antibacterial agents. Mean multiple antibiotic resistance index (MARI) was 0.77 (range=0.42-1.00), 0.58 (range=0.25-0.83), and 0.79 (range=0.58-0.92) for the isolates from beef, chicken meat, and pork samples, respectively. All the isolates had MARI >0.2. Conclusion: Multidrug-resistant Listeria strains contaminate raw beef, pork, and chicken meats marketed in Enugu State, Southeast Nigeria.

scholarly journals Exploring Multidrug Resistant and Possible Extensively Drug Resistant Extended-Spectrum Β-Lactamase-Producing Escherichia coli Isolated from Frozen Chicken Meat in Bangladesh

2020 ◽  
Author(s):  
Mst. Sonia Parvin ◽  
Sudipta Talukder ◽  
Md. Yamin Ali ◽  
Emdadul Haque Chowdhury ◽  
Md. Tanvir Rahman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Biochemical Properties ◽  
Chicken Meat ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Beta Lactamase ◽  
Isolation And Identification ◽  
Disk Diffusion Method ◽  
Extended Spectrum

Multidrug resistant extended-spectrum β-lactamase (ESBL)-producing Escherichia coli is considered a serious concern to public health worldwide including Bangladesh, and chicken meat is recognized as an important reservoir of ESBL-Ec dissemination to humans. Therefore, this study aimed to determine the prevalence, and phenotypic and genotypic antimicrobial resistance pattern of ESBL-producing Escherichia coli (ESBL-Ec) in frozen chicken meat. A total of 113 frozen chicken meat samples were purchased from 40 outlets of 9 branded supershops in five megacities in Bangladesh. Isolation and identification of Escherichia coli were done based on cultural, biochemical properties and PCR assay. The resistance pattern was determined by disk diffusion method. ESBL-encoding genes were determined by multiplex PCR. The results showed that 76.1% samples were positive for Escherichia coli, of which 86% were ESBL producers. All the isolates were multidrug-resistant (MDR). Resistance to 9–11 and 12–13 antimicrobial classes was observed in 38.4% and 17.4% isolates, respectively while only 11.6% were resistant to 3–5 classes. The possible extensively drug resistance (pXDR) was found in 2.3% isolates. The high single resistance was observed for oxytetracycline (93%) and amoxicillin (91.9%), followed by ampicillin (89.5%), trimethoprim-sulphamethoxazole and pefloxacin (88.4%), and tetracycline (84.9%). Most importantly, 89.6% of isolates were resistant to carbapenems. All the isolates were positive for blaTEM gene. However, the blaSHV and blaCTX-M-2 genes were identified in two ESBL-non producer isolates. None of the isolates were carried blaCTX-M-1 gene. This study provided evidence of wide dissemination of MDR and existence of pXDR ESBL-Ec in frozen chicken meat in Bangladesh. Our data clearly indicated that frozen chicken meat is, at the present time, the most significant known food source of ESBL-Ec to which peoples are regularly exposed.

scholarly journals Prevalence of Shiga toxin-producing Escherichia coli isolated from chicken meat in west of Iran

2019 ◽  
Author(s):  
omid zarei ◽  
Leili Shokoohizadeh ◽  
Hadi Hossainpour ◽  
Mohammad Yousef Alikhani
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Genetic Relatedness ◽  
Culture Media ◽  
Chicken Meat ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Meat Samples ◽  
High Level

Abstract Objective: Shiga toxin producing Escherichia coli (STEC) has known as a crucial zoonotic food borne pathogen. A total of 257 row chicken meat samples were collected from different markets in Hamadan city from January 2016 to May 2017. Samples were cultured on selective and differential culture media, and the virulence genes of E. coli isolates were analyzed by PCR assay. The antibiotic resistance patterns of E. coli isolates were determined by disk diffusion method. The genetic relatedness of STEC isolates were analyzed by ERIC-PCR. Results: Totally, 93(36%) of isolates were identified as E. coli in this current study. According serological and microbiological tests, 5(5.3%), 31(33.3%) and 7(7.5%) of E. coli isolates, characterized as Enterohemorrhagic E. coli (EHEC), STEC and attaching and effacing E. coli (AEEC) strains, respectively. High level resistance to tetracycline (89.8), ampicillin (82.8%) and sulfametoxazole-trimotoprime (71%) were detected among E. coli isolates. Analysis of ERIC-PCR results showed five different ERIC types among EHEC isolates. Based on our findings, chicken meat identified as a sources of STEC strains, therefore, the controlling and checkup the chicken meats for the resistance and virulent strains of E. coli should be consider as a crucial issues in public health.

scholarly journals The Prevalence of Shiga Toxin-Producing Escherichia coli and Enteropathogenic Escherichia coli Isolated from Raw Chicken Meat Samples

2021 ◽  
Vol 2021 ◽  
pp. 1-5
Author(s):  
Omid Zarei ◽  
Leili Shokoohizadeh ◽  
Hadi Hossainpour ◽  
Mohammad Yousef Alikhani
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Genetic Relatedness ◽  
Culture Media ◽  
Chicken Meat ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Meat Samples ◽  
High Level

Background. Shiga toxin-producing Escherichia coli (STEC) is known as a crucial zoonotic food-borne pathogen. A total of 257 raw chicken meat samples were collected from different markets in Hamadan, west of Iran, from January 2016 to May 2017. Materials and Methods. The samples were cultured in selective and differential culture media, and the virulence genes of E. coli isolates were analyzed by PCR assay. The antibiotic resistance patterns of E. coli isolates were determined by the disk diffusion method. The genetic relatedness of the E. coli O157 isolates was analyzed by ERIC-PCR. Results. In total, 93 (36% ± 3.12) of the isolates were identified as E. coli in this study. Based on serological and microbiological tests, 36 (38.7% ± 9.9), 7 (7.5% ± 5.35), and 12 (12.9% ± 6.81) of the E. coli isolates were characterized as STEC, enteropathogenic E. coli (EPEC), and attaching and effacing E. coli (AEEC) strains, respectively. A high level of resistance to nalidixic acid (91.4% ± 5.7), tetracycline (89.2% ± 6.31), ampicillin (82.8% ± 7.67), and trimotoprime-sulfametoxazole (71% ± 9.22) was detected among the E. coli isolates. The analysis of the ERIC-PCR results showed five different ERIC types among the E. coli O157 isolates. Conclusions. Based on our findings, control and check-up of poultry meats should be considered as a crucial issue for public health.

Antimicrobial Resistance in Enterococcus spp. Strains Isolated from Organic Chicken, Conventional Chicken, and Turkey Meat: A Comparative Survey

2007 ◽  
Vol 70 (4) ◽  
pp. 1021-1024 ◽  
Author(s):  
J. M. MIRANDA ◽  
M. GUARDDON ◽  
A. MONDRAGÓN ◽  
B. I. VÁZQUEZ ◽  
C. A. FENTE ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Common Species ◽  
Multidrug Resistant ◽  
Chicken Meat ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Turkey Meat ◽  
Enterococcus Spp ◽  
Resistant Strains ◽  
Resistance Data

The mean counts of Enterococcus spp. were determined for 30 samples each of organic chicken meat, conventional chicken meat, and turkey meat, and differences for Enterococcus contamination in meat were determined. Two enterococci strains from each sample were isolated to obtain a total of 180 strains, and resistance to ampicillin, chloramphenicol, doxycycline, ciprofloxacin, erythromycin, gentamicin, nitrofurantoin, and vancomycin was determined by a disk diffusion method. Average counts obtained showed that Enterococcus mean counts from organic chicken meat (3.18 log CFU/g) were significantly higher than those obtained from conventional chicken meat (2.06 log CFU/g) or conventional turkey meat (1.23 log CFU/g). However, the resistance data obtained showed that isolates from organic chicken meat were less resistant than enterococci isolates from conventional chicken meat to ampicillin (P = 0.0067), chloramphenicol (P = 0.0154), doxycycline (P = 0.0277), ciprofloxacin (P = 0.0024), erythromycin (P = 0.0028), and vancomycin (P = 0.0241). In addition, isolates from organic chicken were less resistant than conventional turkey meat isolates to ciprofloxacin (P = 0.001) and erythromycin (P = 0.0137). Multidrug-resistant isolates were found in every group tested, but rates of multidrug-resistant strains were significantly higher in conventional chicken and turkey than those obtained from organic chicken meat. Enterococcus faecalis was the most common species isolated from organic chicken (36.67%), whereas Enterococcus durans was the most common species isolated from conventional chicken (58.33%) and turkey (56.67%). The rates obtained for antimicrobial resistance suggest that although organic chicken meat may have higher numbers of Enterococcus, these bacteria present a lower level of antimicrobial resistance.

Multidrug-Resistant Salmonella Isolates from Swine in the Eastern Cape Province, South Africa

2016 ◽  
Vol 79 (7) ◽  
pp. 1234-1239 ◽  
Author(s):  
CHINWE JULIANA IWU ◽  
BENSON CHUKS IWERIEBOR ◽  
LARRY CHIKWELU OBI ◽  
ALBERTUS KOTZE BASSON ◽  
ANTHONY IFEANYI OKOH
Keyword(s):  
South Africa ◽  
Growth Promotion ◽  
Resistance Index ◽  
Multidrug Resistant ◽  
Farm Animals ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Eastern Cape ◽  
Disk Diffusion Method ◽  
Genes Encoding

ABSTRACT The exposure of farm animals to antimicrobials for treatment, prophylaxis, or growth promotion can select for resistant bacteria that can be transmitted to humans, and Salmonella as an important zoonotic pathogen can act as a potential reservoir of antimicrobial resistance determinants. We assessed the antibiogram profiles of Salmonella species isolated from pig herds in two commercial farms in South Africa. Two hundred fifty-eight presumptive Salmonella isolates were recovered from the fecal samples of 500 adult pigs. Specific primers targeting Salmonella serogroups A, B, C1, C2, and D were used to determine the prevalence of different serogroups. Only serogroup A (n =48) was detected, while others were not. Antimicrobial susceptibility of the confirmed Salmonella serogroup A isolates was performed by using the disk diffusion method against a panel of 18 antibiotics. All the 48 isolates were resistant to tetracycline and oxytetracycline, while 75% were resistant to ampicillin, sulphamethoxazole-trimethoprim, nalidixic acid, and streptomycin. All the isolates exhibited multidrug resistance, with the predominant phenotype being against 11 antibiotics, and multiple antibiotic resistance index ranged between 0.3 and 0.6. The incidence of genes encoding resistance against ampicillin (ampC), tetracycline (tetA), and streptomycin (strA) were 54, 61, and 44%, respectively. We conclude that healthy pigs are potential reservoirs of multidrug-resistant Salmonella that could be transmitted to humans through the food chain and, hence, a significant public health threat.

scholarly journals  Antibiotic resistance of enterococci, coagulase negative staphylococci and Staphylococcus aureus isolated from chicken meat

2013 ◽  
Vol 31 (No. 1) ◽  
pp. 14-19 ◽  
Author(s):  
N.E. Yurdakul ◽  
Z. Erginkaya ◽  
E. Ünal
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Chicken Meat ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Coagulase Negative Staphylococci ◽  
Disk Diffusion Method ◽  
Enterococcus Spp ◽  
Meat Samples ◽  
And Staphylococcus Aureus

We determined the antibiotic resistance of enterococci, coagulase negative staphylococci, and Staphylococcus aureus isolated from chicken meat samples. The antibiotic resistance of the isolated strains was estimated by the Kirby-Bauer disk diffusion method (according to the NCCLS document M2-A9 suggestions). It was found that all strains of Enterococcus spp. were resistant to tetracycline, 75% of them were resistant to ciprofloxacin, and 50% of them were resistant to erythromycin, vancomycin, and chloramphenicol. Also all strains of S. aureus were resistant to tetracycline and 25% of S. aureus strains were resistant to erythromycin and chloramphenicol, whereas all strains of S. aureus were sensitive to teicoplanin and 25% of them were sensitive to vancomycin and ciprofloxacin. As for the isolate of coagulase negative staphylococci (CNS), 68.1% of them were resistant to erythromycin, 77.2% of them were resistant to tetracycline, 59% of them were resistant to vancomycin, 9% of them were resistant to teicoplanin, and 27.2% of them were resistant to both chloramphenicol and ciprofloxacin. As a result, it was found that most of the strains (all of S. aureus and Enterococcus spp., also 77.2% CNS) were resistant to tetracycline.  

scholarly journals Comparison of antibiotic resistance pattern among Enteropathogenic bacteria isolated from broiler and backyard chicken meat

2019 ◽  
Vol 44 (2) ◽  
pp. 228
Author(s):  
R. Yulistiani ◽  
D. Praseptiangga ◽  
S. Supyani ◽  
S. Sudibya
Keyword(s):  
Antibiotic Resistance ◽  
Multidrug Resistant ◽  
Chicken Meat ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Salmonella Spp ◽  
Disk Diffusion Method ◽  
Broiler Meat ◽  
Resistant Strains ◽  
Backyard Chicken

This study aimed to compare the antibiotic resistance patterns among original Enterobacteriaceae isolates from broiler and backyard chicken meats in Surabaya, Indonesia, isolated in 2016-2017. The Kirby–Bauer disk diffusion method was used to determine the resistance of these isolates against tetracycline (TE), gentamicin (CN), cefoxitin (FOX), sulfamethoxazole-trimethoprim (SXT), nalidixic acid (NA), and chloramphenicol (C). Both broiler and backyard chicken meat isolates were resistant to the six antibiotics tested. Overall, broiler meat isolates which resistant to TE, CN, FOX, SXT, NA, C were 57.76% higher than backyard chicken meat isolates. More than 50% of broiler meat isolates (304 samples) were resistant to TE and NA, whereas backyard chicken meat isolates (310 samples) were only resistant to TE. The resistant strains found in both meat isolates were Salmonella spp., Escherichiacoli, Shigella spp., Citrobacter spp., Klebsiella spp., Yersinia spp., Proteus spp., Enterobacter spp., Serratia spp., and Edwardsiella spp. Resistant strains of broiler meat isolates were significantly higher (P<0.05) than backyard chicken meat isolates, except Edwardsiella spp. Overall, multidrug-resistant Enterobacteriaceae was found to be higher in broiler meat isolates than in backyard chicken meat isolates. Broiler and backyard chicken meats are potential reservoirs of multidrug-resistant Enterobacteriaceae which threat to public health.

Virulence Properties of Extended Spectrum β-Lactamase–Producing Klebsiella Species in Meat Samples

2011 ◽  
Vol 74 (4) ◽  
pp. 559-564 ◽  
Author(s):  
NESLIHAN GUNDOGAN ◽  
SUMRU CITAK ◽  
EMEL YALCIN
Keyword(s):  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Serum Resistance ◽  
Esbl Production ◽  
Disk Diffusion Method ◽  
Klebsiella Species ◽  
Extended Spectrum ◽  
Meat Samples ◽  
Virulence Properties

The present study was carried out to identify virulence properties (siderophores, serum resistance, and hemolysin) and antibiotic resistance in extended spectrum β-lactamase (ESBL)–producing Klebsiella isolates from 60 calf and chicken meat samples purchased from various supermarkets in Ankara, Turkey. Of the 45 Klebsiella isolates, 24 (53%) were identified as K. oxytoca and 21 (47%) were identified as K. pneumoniae. A high proportion of Klebsiella isolates had virulence factors such as hemolytic activity (67%), siderophore production (44%), and serum resistance (38%). The double-disk synergy test was used to determine ESBL production. ESBL production was detected in 13 (29%) of the 45 Klebsiella isolates. Resistance to 14 antimicrobials was tested in all Klebsiella isolates by the disk diffusion method. All isolates were resistant to two or more antimicrobial agents. All ESBL-producing Klebsiella isolates were highly resistant to cephalosporins and monobactams. Our findings indicate that meat and its products represent potential hazardous sources of multidrug-resistant and virulent Klebsiella species.

scholarly journals Resistensi Antibiotik pada Escherichia coli yang Diisolasi dari Daging Ayam pada Pasar Tradisional di Kota Bogor (ANTIBIOTIC RESISTANCE IN ESCHERICHIA COLI ISOLATED FROM CHICKEN MEAT OF TRADITIONAL MARKETS IN THE CITY OF BOGOR)

2019 ◽  
Vol 20 (1) ◽  
pp. 125
Author(s):  
Connie Januari ◽  
Mirnawati Bachrum Sudarwanto ◽  
Trioso Purnawarman
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Bacterial Resistance ◽  
Antibiotic Use ◽  
Multidrug Resistant ◽  
Chicken Meat ◽  
Diffusion Method ◽  
Negative Effects ◽  
Disk Diffusion Method ◽  
E Coli

Antibiotic use in farm is spread widely to treat of poultry disease including therapy, supportive or preventive use and as afeed additive to improve chicken performance. The negative effects of antibiotic use can increase the level of bacterial resistance to antibiotics. This study aimed to investigate on antibiotic resistance in Escherichia coli isolated from chicken meat that were sold in Traditional Market of Bogor City. A total of 175 samples of chicken meat were taken by purposive sampling method, out of 175 found 50 positive samples of E. coli. The samples were subjected to E. coli examination and the isolated E. coli were tested for the antibiotic resistance using eight antibiotics, i.e., amoxicillin, cefotaxime, colistin, nalidixid acid, streptomycin, erythromycin, oxytetracillin, and tetracycline. The study was conducted by using the disk diffusion method on Muller-Hinton agar according to the Clinical and Laboratory Standards Institute guidelines. The study showed E. coli isolated from chicken meat were resistance towards amoxicilin (90%), colistin (94%), nalidixid acid (86%), streptomycin (98%), erythromycin (98%), oxytetracillin (84%), tetracycline (86%), and cefotaxime antibiotics (12%). The proportion of multidrugresistant was 99%. The higher of multidrug-resistant indicated the E. coli would be a threat to public and environmental health. 

Prevalence of Antibiotic-Resistant Escherichia coli Isolates from Local and Imported Retail Chicken Carcasses

2020 ◽  
Vol 83 (12) ◽  
pp. 2200-2208
Author(s):  
NAHLA O. ELTAI ◽  
HADI M. YASSINE ◽  
TAHRA EL-OBEID ◽  
SARA H. AL-HADIDI ◽  
ASMAA A. AL THANI ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Chicken Meat ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Multiplex Pcr Assay ◽  
Synergy Test

ABSTRACT The spread of antibiotic resistance among bacterial strains has been associated with consumption of food contaminated with both pathogenic and nonpathogenic bacteria. The objective of this study was to determine the prevalence of antibiotic resistant Escherichia coli isolates in local and imported retail raw chicken meat in Qatar. A total of 270 locally produced (chilled) and imported (chilled or frozen) whole chicken carcasses were obtained from three Hypermarket stores in Qatar. The 216 E. coli isolates recovered from the chicken samples were subjected to antibiotic susceptibility testing with the disk diffusion method. Extended-spectrum β-lactamase (ESBL) production was evaluated with the double disk synergy test. Isolates harboring colistin resistance were identified with a multiplex PCR assay and DNA sequencing. Nearly 89% (192) of the 216 isolates were resistant to at least one of the 18 antibiotics tested. Isolates from local and imported chicken carcasses had relatively higher resistance to sulfamethoxazole (62% of isolates), tetracycline (59.7%), ampicillin and trimethoprim (52.3% each), ciprofloxacin (47.7%), cephalothin (45.4%), and colistin (31.9%). Less resistance was found to amoxicillin–clavulanic acid (6%), ceftriaxone (5.1%), nitrofurantoin (4.2%), piperacillin-tazobactam (4.2%), cefepime (2.3%), meropenem (1.4%), ertapenem (0.9%), and amikacin (0.9%). Nine isolates (4.2%) were ESBL producers, and 137 (63.4%) were multidrug resistant. The percentages of multidrug-resistant, ESBL-producing, and colistin resistant isolates were significantly higher among isolates from local chilled than from imported chilled and frozen chicken samples. Our findings indicate the high prevalence of antibiotic-resistant E. coli in chicken meat sold at retail in Qatar. HIGHLIGHTS

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