Research on the Inhibitory Activity of Endophytic Fungi in Huperzia serrata against AchE

2013 ◽  
Vol 303-306 ◽  
pp. 2598-2601
Author(s):  
Na Li ◽  
Zi Ping Zhu
Keyword(s):  
Endophytic Fungi ◽  
Active Ingredient ◽  
Inhibitory Activity ◽  
Bacterial Strain ◽  
Total Alkaloid ◽  
Inhibitory Concentration ◽  
Fermentation Broth ◽  
Inhibition Activity ◽  
Fermentation Products ◽  
Huperzia Serrata

Twenty-two endophytic fungi in the Huperzia serrata were separated to test their fermentation broth. Research found that seven of them could produce alkaloid. Of these, the fermentation products from the T4 bacterial strain produced the most, with 2.027 mg/g. Ellman colorimetry has determined that the fermentation broth in four fungi had inhibition activity against AchE. It also showed that the total alkaloid of the T17 bacterial strain had a half-inhibitory concentration (IC50) of 98.494 μg•mL-1 on the mouse’s brain against AchE. This yielded the conclusion that the total alkaloid in the T17 fermentation broth restrains AchE effectively, and that it further contributes to the separation of its active ingredient.

scholarly journals Isolation of Endophytic Fungi QPS 05 from Quercus phillyraeoides A. Gray and Its Potential for α-Glucosidase Inhibitory Activity

2018 ◽  
Vol 20 (1) ◽  
pp. 1-7
Author(s):  
Anastasia Wheni Indrianingsih ◽  
Amalia Indah Prihantini ◽  
Sanro Tachibana
Keyword(s):  
Fatty Acids ◽  
Endophytic Fungi ◽  
Inhibitory Activity ◽  
Endophytic Fungus ◽  
Inhibitory Concentration ◽  
Life Cycles ◽  
Ic50 Values ◽  
Quercus Phillyraeoides ◽  
Alternaria Sp ◽  
Harmful Effects

AbstractEndophytic fungi are the microorganisms that spend all or part of their life cycles within plant tissue without causing harmful effects on the plant. In this study, 14 endophytic fungus from Quercus phillyraeoides A. Gray were isolated. Alternaria sp. QPS 05, an endophytic fungi which was isolated from the stem of Q. phillyraeoides A. Gray showed the highest α-glucosidase inhibitory activity. Further separation of ethyl acetate extract from the fungus led to the isolation of active substance from hexane-soluble fraction which give fatty acids mixture consist of palmitic acid, oleic acid, linoleic acid and linolenic acid (1) strong inhibitory activity against α-glucosidase. Isolated fatty acids (1) had inhibitory concentration (IC50) values against Saccharomyces cerevisiae was 12.10 μg/mL. The results of the present study showed that endophytic fungus from Alternaria sp. QPS 05 potentially contained a rich source of natural antidiabetic medicine.

scholarly journals The development of a novel and powerful biosynthesis platform for expressing huperzine A for the Alzheimer's disease

2020 ◽  
Author(s):  
Han Wen-Xia ◽  
Han Zhong-Wen ◽  
Jia Min ◽  
Zhang Han ◽  
Li Wei-Ze ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Liquid Chromatography ◽  
Physicochemical Properties ◽  
Endophytic Fungi ◽  
Morphological Characteristics ◽  
Huperzine A ◽  
Nuclear Ribosomal Dna ◽  
Inhibition Activity ◽  
Huperzia Serrata

Abstract Background Huperzine A is an important drug for treating Alzheimer's disease and mainly extracted from the Huperzia serrata . Nevertheless, the content of Huperzine A in Huperzia serrata is very low of 0.007% with growing circle of 8 to 10 years, and the chemical synthesis of Huperzine A still has some insurmountable limitations in the industrialized production. So, the available resources of Huperzine A for clinical treatment of Alzheimer's disease are scarce. The purpose of this work was to construct a biosynthesis platform based on the endophytic fungi from Huperzia serrata . Methods Based on the morphological characteristics and nuclear ribosomal DNA ITS sequences of endophytic fungi to complete the strain identification. Combined alkaloid precipitation with acid dye colorimetry, thin layer chromatography, high-performance liquid chromatography, liquid chromatography-tandem mass spectrometry analysis and inhibition activity of acetylcholinesterase determination model to determine the physicochemical properties of the biosynthetic products. Compare the biosynthetic HupA with the listed APIs of HupA by the test of AChE inhibition ability and cytotoxicity in vitro. Results In this work, five endophytic fungi Mucor racemosus NSH-D, Mucor fragilis NSY-1, Fusarium verticillioides NSH-5, Fusarium oxysporum NSG-1 and Trichoderma harzianum NSW-V were firstly found and isolated from the Chinese folk medicine Qian Ceng Ta ( Huperzia serrata (Thunb.) Trevis. (Lycopodiaceae)), which were identified according to their morphological characteristics and nuclear ribosomal DNA ITS sequences. These fungi could effectively biosynthesize huperzine A in liquid culture of 100-400 mg/L which were 1 000 times higher than that of other reported conventional endophytic fungi. Moreover, these fungi with higher hereditary stability could possess the initial express ability of HupA after 40 generations, and the expressed HupA from these biosynthesis systems has the prior physicochemical properties, better inhibition activity of acetylcholinesterase and lower cytotoxicity compared to the listed APIs of HupA. Conclusions These results indicate that the endophytic fungi in this work provide a promising alternative platform for producing HupA at industrial scale by biosynthesis for the treatment of Alzheimer's disease.

Study on Active Ingredient of Blueberry Pomace in Fermentation by Lentinus edodes Hypha

2014 ◽  
Vol 926-930 ◽  
pp. 238-241
Author(s):  
Li Guo ◽  
Peng Wang
Keyword(s):  
Gallic Acid ◽  
Active Ingredient ◽  
Ellagic Acid ◽  
Acid Content ◽  
Hplc Method ◽  
Spectrophotometric Analysis ◽  
Anthocyanin Content ◽  
Lentinus Edodes ◽  
Flavonoid Content ◽  
Fermentation Products

The effect of fermentation by Lentinus edodes hypha on active ingredient of blueberry pomace was studied, and the changes of fermentation products with protein, flavonoid and anthocyanin content were evaluated by spectrophotometric analysis. The protein content decreased slowly, and the flavonoid content in the first 66 h increased the highest level 0.09 mg/g, the anthocyanin content decreased during the first fermentation process, then increased to 0.568 mg/g at 30 h. The fermentation product of ellagic acid and gallic acid content were analyzed by using HPLC Method, the ellagic acid content decreased, and the gallic acid content increased first and then decreased, reached the highest value for the 0.310 mg/g in the first 54 h.

scholarly journals ALPHA AMYLASE AND ALPHA GLUCOSIDASE INHIBITION ACTIVITY OF SELECTED EDIBLE SEAWEEDS FROM SOUTH COAST AREA OF INDIA

2017 ◽  
Vol 9 (6) ◽  
pp. 64 ◽  
Author(s):  
P. Reka ◽  
Thahira Banu A. ◽  
M. Seethalakshmi
Keyword(s):  
Aqueous Extract ◽  
Inhibitory Activity ◽  
Alpha Amylase ◽  
Postprandial Blood Glucose ◽  
Inhibition Activity ◽  
Gracilaria Edulis ◽  
Edible Seaweeds ◽  
Daily Diet ◽  
Postprandial Blood Glucose Level ◽  
Alpha Glucosidase

Objective: The present work was to investigate the alpha amylase and alpha-glucosidase inhibitory activity of the selected edible seaweeds.Methods: The seaweeds namely Acanthophora spicifera, Gracilaria corticata, Gracilaria edulis, Ulva lactuca and Ulva reticulata were selected for this study. Six and eight hours of ethanol and aqueous extract were used for the estimation of alpha amylase using DNS method and alpha-glucosidase inhibition activity.Results: The study reported that the solvent from ethanol and aqueous in eight hours of extraction showed a higher inhibitory activity than six hours of extraction. Maximum of 89.1±0.96 and 79.55±3.08 percent of alpha-amylase and alpha-glucosidase inhibition activity were detected in the eight hours of aqueous extract (0.5 ml) of Ulva reticulata and Gracilaria edulis respectively. All the selected edible seaweeds had significant differences (p<0.05) in alpha amylase and alpha glucosidase inhibition activity between the selected seaweeds with different extracts.Conclusion: It was concluded that all the selected edible seaweeds have the potential to act as a potent inhibitor of the carbohydrate hydrolyzing enzyme. Thus, it was clear from the study that seaweeds incorporated in small amounts in the dishes consumed in the daily diet can bring a control on postprandial blood glucose level.

scholarly journals Isolation and antimicrobial effects of endophytic fungi from Edgeworthia chrysantha

2015 ◽  
Vol 10 (3) ◽  
pp. 529 ◽  
Author(s):  
Huawei Zhang ◽  
Chuanfeng Ruan ◽  
Xuelian Bai
Keyword(s):  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Fermentation Broth ◽  
Fungal Endophytes ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Human Pathogens ◽  
Antimicrobial Effects ◽  
Staphyloccocus Aureus

<p>Ten fungal strains isolated from <em>Edgeworthia chrysantha</em>, one of traditional medicinal plants in China, were evaluated their antimicrobial activities against three human pathogens, <em>Escherichia coli, Staphyloccocus aureus and Candida albicans</em>, and two phytopathogens, <em>Rhizoctonia cerealis</em> and <em>Colletotrichum gloeosporioides</em>. The results indicated that most ethyl acetate extracts of fermentation broth of these fungal endophytes had stronger antimicrobial activities than their fermentation broth. Among these endophytic strains, both fermentation broth and the ethyl acetate extract of strain D showed the strongest inhibitory effects on all pathogens. Strains 5-19 and BZ also exhibited potent antibacterial activities. However, other strains had weak or no antimicrobial effect. This was the first report on the isolation and antimicrobial effects of endophytic fungi from <em>E. chrysantha</em>.   </p><p> </p>

scholarly journals Prevalence of Tonsillitis caused by Streptococcus spp. Among children and the effect of some Lactic acid bacterial (LAB) strain on it

2016 ◽  
Vol 13 (2) ◽  
pp. 218-227
Author(s):  
Baghdad Science Journal
Keyword(s):  
Inhibitory Activity ◽  
Pathogenic Bacteria ◽  
Biochemical Characterization ◽  
Bacterial Isolate ◽  
Inhibition Zone ◽  
Laboratory Culture ◽  
Diffusion Method ◽  
Inhibition Activity ◽  
Str System

In this study 100 samples were collected from infected children with acute and chronic tonsillitis who attended to Al-Yarmook Teaching Hospital (ENT consultation clinic) from 5/12/2013 to 1/3/2014. The result of laboratory culture was positive in 67 samples. Depending on their cultural, morphological and biochemical characterization of bacterial isolate of them were identified as (37.31%) belonged to Streptococcus pyogenes and the diagnosis is confirmed by the use of Remel Rapid STR System, (34.32%) belonged to S.parasanguinis, (11.94%) S.mitis, (11.94%) S.oralis and (4.47%) S.thoraltensis . Results confirmed that cup assay gave highest inhibition zone after 24 hrs compare with well diffusion methods for suspension of L.acidophilus gave highest inhibition zone after 48 hrs for incubation, while ahigh inhibition zone revealed for suspension of L.fermentum after 24 hrs incubation. the study included also the measurement of the inhibition activity for bacteriocins produced by L.acidophilus bacteria against pathogenic bacteria on nutrient agar by well diffusion method in which results revealed stability of the bacteriocins produced towards PH which kept its activity with PH 4-6 for 24 hrs, and the highest stability was with PH 4, however it lost a lot of its activity with acidic PH less than 2 and alkaline PH as 8. The treatment of bacteriocins with salts such as Nacl it revealed little effect in inhibition zone within 1 & 2% concetrations. The salt MgSo4 & Kcl showed reduction in the inhibitory activity in the low concentration, however the higher concentration of salt caused great reduction and 5% concentration led to loss of inhibitory activity for bacteriocins completely.

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