scholarly journals Steatocrit: A Simple Method for Detection Fat Malabsorption

2019 ◽  
Vol 32 (11-12) ◽  
pp. 298-303
Author(s):  
Suharyono Suharyono ◽  
Hindra Irawan Satari ◽  
Agus Firmansyah
Keyword(s):  
Nutritional Status ◽  
Chronic Diarrhoea ◽  
Absorption Test ◽  
Technical Expertise ◽  
Simple Method ◽  
Female Patients ◽  
Alternative Method ◽  
Fat Malabsorption

Steatocrlt was determined through microcentrifugation of fecal hemogenate from 45 patients with chronic diarrhoea. In the same patients urine materials were collected to determine fat malabsorption using Lipiodol absorption test. There were 28 male and 17 female patients. Severe malabsorption using steatocrit was detected tn 31 patients (68;9%), while LAT determined severe malabsorption in 34 patients (75.5%). The overall sensitivity was 88.2%, and spesificity was 90.9%. Nutritional status did not influence the sensitivity and spesificity of steatocrit. We propose that this simple semiquantitative test can be used as an alternative method for detecting fat malabsorption particularly in laboratories with limited technical expertise.

scholarly journals The nutritional status of adult female patients with disabilities in Kuwait

2018 ◽  
Vol 13 (3) ◽  
pp. 238-246
Author(s):  
Dalal U. Alkazemi ◽  
Maryam H. Zadeh ◽  
Tasleem A. Zafar ◽  
Stan J. Kubow
Keyword(s):  
Nutritional Status ◽  
Adult Female ◽  
Female Patients

scholarly journals Penilaian gizi sederhana: alat penilaian praktis untuk menentukan status gizi pasien rawat inap di rumah sakit

2005 ◽  
Vol 1 (3) ◽  
pp. 137
Author(s):  
Ida Mardalena ◽  
Bambang Suprapto ◽  
Widarto Widarto ◽  
Bhisma Murti
Keyword(s):  
Nutritional Status ◽  
Screening Tool ◽  
Nutritional Assessment ◽  
High Sensitivity ◽  
Hospitalized Patients ◽  
Simple Method ◽  
High Incidence ◽  
Time Required ◽  
Sensitivity Specificity ◽  
Efficient Screening

Background: Absence of effective and efficient screening tool for assessment of nutritional status have lead to high incidence of malnutrition among hospitalized patients.Objective: To develope practical screening tool for assessing nutritional status of hospitalized patients.Method: A simple method of nutritional assessment, called The Simple Nutritional Assessment, was compared to albumin serum as the gold standard. This study involved 50 nurses and 50 hospitalized patients in Dr. Moewardi General Hospital, Surakarta.Result: There was significant concordance of nutritional status assessed by the Simple Nutritional Assessment and the level of albumin serum (k=0,92). Sensitivity, specificity, and likelihood ratio of the Simple Nutritional Assessment compared to serum albumin were 92%, 67–75%, and 3.8, respectively. Internal consistence of the Simple Nutritional ssessment is high (Alpha=0.80). Average time required for doing the assessment was only 5 minutes.Conclusion: The Simple Nutritional Assessment can be used for quick screening of nutritional status of hospitalized patients with high sensitivity and reliability.

scholarly journals A Fast and Accessible Method for the Isolation of RNA, DNA, and Protein to Facilitate the Detection of SARS-CoV-2

2020 ◽  
Author(s):  
Jose Carlos Ponce-Rojas ◽  
Michael S. Costello ◽  
Duncan A. Proctor ◽  
Kenneth S. Kosik ◽  
Maxwell Z. Wilson ◽  
...  
Keyword(s):  
Low Cost ◽  
Rna Extraction ◽  
Genetic Material ◽  
Simple Method ◽  
Dna And Rna ◽  
Alternative Method ◽  
Accessible Method ◽  
Comparable Performance ◽  
Viral Envelopes ◽  
Common Laboratory

AbstractManagement of the COVID-19 pandemic requires widespread SARS-CoV-2 testing. A main limitation for widespread SARS-CoV-2 testing is the global shortage of essential supplies, among these, RNA extraction kits. The need for commercial RNA extraction kits places a bottleneck on tests that detect SARS-CoV-2 genetic material, including PCR-based reference tests. Here we propose an alternative method we call PEARL (Precipitation Enhanced Analyte RetrievaL) that addresses this limitation. PEARL uses a lysis solution that disrupts cell membranes and viral envelopes while simultaneously providing conditions suitable for alcohol-based precipitation of RNA, DNA, and proteins. PEARL is a fast, low-cost, and simple method that uses common laboratory reagents and offers comparable performance to commercial RNA extraction kits. PEARL offers an alternative method to isolate host and pathogen nucleic acids and proteins to streamline the detection of DNA and RNA viruses, including SARS-CoV-2.

scholarly journals SP676NEW AND SIMPLE METHOD FOR ASSESSMENT OF NUTRITIONAL STATUS

2018 ◽  
Vol 33 (suppl_1) ◽  
pp. i573-i573
Author(s):  
Peter Marckmann ◽  
Mette Koefoed
Keyword(s):  
Nutritional Status ◽  
Simple Method

scholarly journals Modification of a Rapid Method for the Identification of Gene-Specific Polymorphisms inCryptosporidiumparvum and Its Application to Clinical and Epidemiological Investigations

2001 ◽  
Vol 67 (12) ◽  
pp. 5581-5584 ◽  
Author(s):  
K. Elwin ◽  
R. M. Chalmers ◽  
R. Roberts ◽  
E. C. Guy ◽  
D. P. Casemore
Keyword(s):  
Gene Locus ◽  
Protein Gene ◽  
Fragment Length Polymorphism ◽  
Analytical Techniques ◽  
Technical Expertise ◽  
Epidemiological Investigation ◽  
Simple Method ◽  
Adhesive Protein ◽  
Clinical Investigations ◽  
Pcr Rflp

ABSTRACT The application of genotyping to clinical isolates ofCryptosporidium has increased significantly our knowledge and understanding of the distribution and epidemiology of this parasite. However, some methods can be laborious and demand specialist technical expertise. PCR-restriction fragment length polymorphism (RFLP) techniques represent a more rapid and simple method of genotyping to support epidemiological and clinical investigations than conventional DNA analytical techniques. We describe a nested PCR-RFLP technique that identifies polymorphisms in theC. parvum thrombospondin-related adhesive protein gene locus; this method offers a sensitive and specific tool for the confirmation and investigation of disease associated withC. parvum. The potential of this enhanced method is demonstrated by its application to the confirmation and epidemiological investigation of an outbreak of cryptosporidiosis associated with a school visit to an open farm.

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