ANTIRADICAL ACTIVITY OF THE ESSENTIAL OIL AND WATER-ALCOHOL EXTRACTS OF ORIGANUM VUL-GARE L., GROWING IN THE KRASNOYARSK TERRITORY

The antiradical properties of essential oil and water-alcohol extracts of oregano (Origanum vulgare L.), which grows in the Krasnoyarsk territory, were studied in model reactions with a free stable 2,2-diphenyl-1-picrylhydrazyl radical. Essential oil is obtained by exhaustive hydro-distillation. Separate fractions of oil: first 10 minutes from the start of the distillation, the second – after 20 min, the third after 40 min, and the fourth in 80 minutes, a fifth fraction was collected after the end of hydroponically. The results of the DPPH test showed that all the studied samples of essential oil exhibit anti-radical activity (ARA), the values of which increase from 30.4% (the first fraction) to 51.0% (the fifth). The ARA of O. vulgare whole oil was 36.1%. The ARA values of O. vulgare water-alcohol extracts vary from 56.6 to 100% depending on the alcohol concentration and the volume of the added extract. The highest anti-radical activity is 70% extract, and the lowest – 96%. According to the ARA value, the studied extracts and essential oil are O. vulgare, which grows in the Siberian region, can be arranged in the following order: 70% water-alcohol extract > 40% extract > 20% extract > water extract > 96% alcohol extract > essential oil.

Download Full-text

ANTIRADICAL ACTIVITY OF EXTRACTIVES FROM THE ACHILLEA MILLEFOLIUM L. OF THE SIBERIAN REGION

chemistry of plant raw material ◽

10.14258/jcprm.2021028888 ◽

2021 ◽

pp. 129-135

Author(s):

Aleksandr Alekseyevich Efremov ◽

Irina Dement'yevna Zykova

Keyword(s):

Essential Oil ◽

Steam Distillation ◽

Antiradical Activity ◽

Achillea Millefolium ◽

Alcoholic Extract ◽

Siberian Region ◽

Alcohol Content ◽

Antiradical Properties ◽

Water Alcohol ◽

Model Reactions

In model reactions with a free stable 2,2-diphenyl-1-picrylhydrazyl radical, the antiradical properties of extractives of common yarrow (Achillea millefolium L.) growing in the Krasnoyarsk Territory were studied: water-alcohol extracts with an alcohol content of 20, 40 and 70% and essential oil. Whole essential oil is obtained by exhaustive hydro-steam distillation for 11 hours. In addition, separate oil fractions were obtained: the first after 20 minutes from the start of distillation, the second after the next 50 minutes, the third after the next 180 minutes, and the fourth after the next 360 minutes. The results of the DPPH test showed that ARA is minimal for alcohol-soluble substances and increases for aqueous-alcoholic extracts from 20.3% in the case of a 20% extract to 21.8% for a 70% aqueous-alcoholic extract. ARA of the obtained samples of essential oil surpasses the ARA values of aqueous, alcoholic and aqueous-alcoholic extracts. It was found that the APA value for 1 fraction of oil is 29.7% and increases with the transition to subsequent fractions to 54.3%. Whole Yarrow essential oil has an ARA value of 52.8%. According to the ARA value, the extractives of yarrow can be arranged in the following row: whole essential oils> 70% aqueous-alcoholic extract> 40% extract> 20% extract> aqueous extract> 96% alcoholic extract.

Download Full-text

ANTIRADICAL ACTIVITY OF SEPARATE FRACTIONS OF ESSENTIAL OIL OF ACORUS CALAMUS ROOTS

chemistry of plant raw material ◽

10.14258/jcprm.2020026659 ◽

2020 ◽

pp. 73-78

Author(s):

Irina Dement'yevna Zykova ◽

Aleksandr Alekseyevich Efremov ◽

Liliya Viktorovna Naimushina

Keyword(s):

Essential Oil ◽

Antiradical Activity ◽

Component Composition ◽

Biologically Active ◽

Acorus Calamus ◽

Siberian Region ◽

Antiradical Properties ◽

Main Components ◽

First Time ◽

Content Of Oxygen

Essential oil from the roots of calamus marsh (Acorus calamus L) was isolated by the method of exhaustive hydroparodistillation, growing on the Krasnoyarsk territory. Separate fractions of essential oil were obtained depending on the duration of isolation. The component composition of the essential oil, the main components of which are acorenone, butyrate α-terpineol, germacrene B, camphor. The antiradical properties of the essential oil of A. сalamus roots growing in the Siberian region, as well as the antiradical properties of its individual fractions were studied for the first time in order to determine the most promising samples as sources of biologically active complexes. To determine the antiradical activity, the reaction of the essential oil components with a stable free 2,2-diphenyl-1-picrylhydrazyl radical was used. The antiradical activity of all studied samples of essential oils was established. There was an increase in antiradical activity with a decrease in the content of monoterpenes in the composition of the essential oil. The lowest antiradical activity (17.5%) was shown by the fraction of essential oil with a high content of monoterpenes, the highest (~100%) – fractions with a high content of oxygen-containing compounds. It was revealed that the ability of components of individual fractions of A. сalamus essential oil to inhibit DPPH radicals is comparable with the antiradical activity of ascorbic acid solutions of equivalent concentration.

Download Full-text

ANTIRADICAL ACTIVITY OF ESSENTIAL OILS OF FILIPENDULA ULMARIA (L.) MAXIM, HYPERICUM PERFORA-TUM L. AND PULMONARIA MOLLIS WULFEN EX HORNEM OF THE KRASNOYRSK TERRITORI FLORA

chemistry of plant raw material ◽

10.14258/jcprm.2021039195 ◽

2021 ◽

pp. 211-217

Author(s):

Irina Dement'yevna Zykova ◽

Aleksandr Alekseyevich Efremov

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Antiradical Activity ◽

Component Composition ◽

Salicylic Aldehyde ◽

Filipendula Ulmaria ◽

Antiradical Properties ◽

Hypericum Perforatum L ◽

Butyl Phthalate ◽

Main Components

The antiradical properties of essential oils from the inflorescences of Filipendula ulmaria (L). Maxim, herbages Hypericum perforatum L. and Pulmonaria mollis Wulfen ex HORNEM., growing on the territory of the Krasnoyarsk territory were studied. For this purpose, the reaction of essential oil components with a stable free 2,2-diphenyl-1-picrylhydrazyl radical was used. Essential oil of the plants under study received comprehensive hydroponically. The component composition of the oils was determined by chromatography-mass spectrometry. The main components of essential oil of F. ulmaria inflorescences are methyl salicylate (28.2%), salicylic aldehyde (2.8 %) and linalool (4.9%), essential oil of H. perforatum – γ - amorphene (30.7%), δ-cadinen (7.1%), (E, E)-β-farnesene (5.5%), caryophyllene (5.0%), ledol (5.0%), essential oil of P. mollis – di-n-butyl phthalate (18.7%), docosan (13.4%), tetracosan (11.6 %). The results of the DPPH test showed that the essential oils of the inflorescences of F. ulmaria and the aboveground part of H. perforatum and P. mollis exhibit antiradical activity (ARA). According to the size of the ARA of essential oils, the studied plants can be arranged in the following row: P. mollis > F. ulmaria > H. perforatum.

Download Full-text

The feasibility of pomegranate beverage enrichment with jujube extract

International Multidisciplinary Research Journal ◽

10.25081/imrj.2017.v7.3361 ◽

2017 ◽

Author(s):

Afshin Jafarpour

Keyword(s):

Sugar Content ◽

Water Extract ◽

Total Solid ◽

Pomegranate Juice ◽

Total Acidity ◽

Alcohol Extract ◽

Extract Concentration ◽

Anti Oxidant ◽

Concentration Levels ◽

Water Alcohol

Jujube consists of Vitamin C, Riboflavin and Thiamin. Vitamin and minerals of fruit are good for heart and improve metabolism. This study evaluates the effects of jujube extract on physicochemical properties and anti-oxidant activity of pomegranate juice. Pomegranate juice was treated with jujube extract (water, alcohol, and water-alcohol) at five concentration levels (5, 10, 15, 20, 25%). Color, total acidity, pH, total solid, activity and anti-oxidant capacity, ash, density, formalin value, tannin and sugar content of treatments were evaluated. The results showed that by increase of concentration of jujube extract, pH was increased and acidity was reduced. By increase of water extract concentration in formulation, anti-oxidant capacity was increased significantly (P<0.05). The highest and lowest anti-oxidant capacity was dedicated to the samples with 25% water extract and 25% alcohol extract.

Download Full-text

Chemical Composition, Olfactory Evaluation and Antioxidant Effects of an Essential Oil of Origanum Vulgare L. from Bosnia

Natural Product Communications ◽

10.1177/1934578x0800300702 ◽

2008 ◽

Vol 3 (7) ◽

pp. 1934578X0800300 ◽

Cited By ~ 1

Author(s):

Ivanka Stoilova ◽

Stefanie Bail ◽

Gerhard Buchbauer ◽

Albert Krastanov ◽

Albena Stoyanova ◽

...

Keyword(s):

Linoleic Acid ◽

Chemical Composition ◽

Essential Oil ◽

Antiradical Activity ◽

Origanum Vulgare ◽

Emulsion Model ◽

Oregano Oil ◽

Linoleic Acid Emulsion ◽

Oxidized Products ◽

Antioxidant Effects

The chemical composition of an essential oil of oregano ( Origanum vulgare L.) from Bosnia was analyzed by GC/FID and GC-MS. In total, 33 constituents were identified and carvacrol (66.2%), p-cymene (9.1%), γ-terpinene (7.3%) and β-caryophyllene (4.1%) were found to be the main compounds. In addition, olfactory evaluation data of the sample and correlations with the odor attributes of the single components are presented to explain the complex aroma impression of this essential oil. The oil possessed antiradical activity with respect to DPPH radicals, exceeding that of its major phenolic component, carvacrol. The oil also revealed scavenging potential with respect to hydroxyl radicals (OH•), which was superior to that towards the DPPH radical; the respective concentrations needed for 50% inhibition (IC50) were 53.8 μg/mL for DPPH and 0.31 μg/mL for OH•. The superoxide scavenging activity of oregano oil was 82.7% at 50 μg/mL. The oil also demonstrated antioxidant activity in a linoleic acid emulsion model system; at 0.05% concentration it inhibited conjugated dienes formation by 69.0% and the generation of secondary oxidized products from linoleic acid by 73.1 %.

Download Full-text

Composition of the essential oil of Origanum vulgare L. ssp. vulgare from various countries

Planta Medica ◽

10.1055/s-2007-987121 ◽

2007 ◽

Vol 73 (09) ◽

Author(s):

A Raal ◽

E Arak ◽

A Orav

Keyword(s):

Essential Oil ◽

Origanum Vulgare

Download Full-text

Chemical Composition and Active Properties Evaluation of Wild Oregano (Origanum Vulgare) and Ginger (Zingiber Officinale-Roscoe) Essential Oils

Revista de Chimie ◽

10.37358/rc.18.8.6448 ◽

2018 ◽

Vol 69 (8) ◽

pp. 1927-1933 ◽

Cited By ~ 1

Author(s):

Mariana Deleanu ◽

Elisabeta E. Popa ◽

Mona E. Popa

Keyword(s):

Essential Oil ◽

Growth Inhibition ◽

Gallic Acid ◽

Aspergillus Flavus ◽

Zingiber Officinale ◽

Penicillium Expansum ◽

Origanum Vulgare ◽

Zingiber Officinale Roscoe ◽

Oregano Oil ◽

Gallic Acid Equivalent

The compounds in Ginger (Zingiber officinale-Roscoe) essential oil provenience China and wild oregano (Origanum vulgare) essential oil of Romanian origin were identified by GC/MS and their antioxidant and antifungal properties were evaluated. Wild oregano oil was characterized by high content of oxygenated monoterpenes hydrocarbons (84.05%) of which carvacrol was the most abundant (73.85%) followed by b-linalool (3.46%) and thymol (2.29%). Ginger oil had a higher content of sesquiterpene hydrocarbons including zingiberene (31.47%), b-sesquiphellandrene (13.76%), a-curcumene (10.41%), a-farnesene (8.31%) and b-bisabolene (7.55%) but a lower content of oxygenated monoterpenes (7.97%). The high content of oxygenated monoterpens of wild oregano oil is in accordance with total content of polyphenols determined by the Folin�Ciocalteu method (6.71�0.73 mg of gallic acid equivalent per g oil). Ginger oil had only 1.34�0.22 mg gallic acid equivalent per g oil. Wild oregano oils exhibited appreciable in vitro antioxidant activity as assessed by 2, 2`-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and 2,2�-azino-bis (3 ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS). The sample concentration required to scavenge 50% of the DPPH free radicals was 0.76�0.13 mg/mL for wild oregano oil compared to 20.22�2.12 mg/mL for ginger oil. Also, wild oregano oils showed significant inhibitory activity against selected pathogenic fungi (Fusarium oxysporum, Aspergillus flavus and Penicillium expansum). 1�L of oregano oil is sufficient for almost 75% growth inhibition of Aspergillus flavus compared to ginger oil which shows antifungal activity at 240�L for 78% growth inhibition. It can be concluded that wild oregano oil could be used as food preservative in some food products in which Fusarium oxysporum, Aspergillus flavus and Penicillium expansum could grow and have potential to produce health hazards mycotoxines.

Download Full-text

Chemical Composition, Antimicrobial activity, In Vitro Cytotoxicity and Leukotoxin Neutralization of Essential Oil from Origanum vulgare against Aggregatibacter actinomycetemcomitans

Pathogens ◽

10.3390/pathogens9030192 ◽

2020 ◽

Vol 9 (3) ◽

pp. 192 ◽

Cited By ~ 2

Author(s):

Sanae Akkaoui ◽

Anders Johansson ◽

Maâmar Yagoubi ◽

Dorte Haubek ◽

Adnane El hamidi ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Periodontal Diseases ◽

Aggregatibacter Actinomycetemcomitans ◽

Psidium Guajava ◽

Minimal Bactericidal Concentration ◽

Origanum Vulgare ◽

Neutralizing Capacity ◽

Broth Microdilution Method

In this study, the essential oil of Origanum vulgare was evaluated for putative antibacterial activity against six clinical strains and five reference strains of Aggregatibacter actinomycetemcomitans, in comparison with some antimicrobials. The chemical composition of the essential oil was analyzed, using chromatography (CG) and gas chromatography–mass spectrometry coupled (CG–MS). The major compounds in the oil were Carvacrol (32.36%), α-terpineol (16.70%), p-cymene (16.24%), and Thymol (12.05%). The antimicrobial activity was determined by an agar well diffusion test. A broth microdilution method was used to study the minimal inhibitory concentration (MIC). The minimal bactericidal concentration (MBC) was also determined. The cytotoxicity of the essential oil (IC50) was <125 µg/mL for THP-1 cells, which was high in comparison with different MIC values for the A. actinomycetemcomitans strains. O. vulgare essential oil did not interfere with the neutralizing capacity of Psidium guajava against the A. actinomycetemcomitans leukotoxin. In addition, it was shown that the O. vulgare EO had an antibacterial effect against A. actinomycetemcomitans on a similar level as some tested antimicrobials. In view of these findings, we suggest that O.vulgare EO may be used as an adjuvant for prevention and treatment of periodontal diseases associated to A. actinomycetemcomitans. In addition, it can be used together with the previously tested leukotoxin neutralizing Psidium guajava.

Download Full-text