ANTIRADICAL ACTIVITY OF EXTRACTIVES FROM THE ACHILLEA MILLEFOLIUM L. OF THE SIBERIAN REGION

In model reactions with a free stable 2,2-diphenyl-1-picrylhydrazyl radical, the antiradical properties of extractives of common yarrow (Achillea millefolium L.) growing in the Krasnoyarsk Territory were studied: water-alcohol extracts with an alcohol content of 20, 40 and 70% and essential oil. Whole essential oil is obtained by exhaustive hydro-steam distillation for 11 hours. In addition, separate oil fractions were obtained: the first after 20 minutes from the start of distillation, the second after the next 50 minutes, the third after the next 180 minutes, and the fourth after the next 360 minutes. The results of the DPPH test showed that ARA is minimal for alcohol-soluble substances and increases for aqueous-alcoholic extracts from 20.3% in the case of a 20% extract to 21.8% for a 70% aqueous-alcoholic extract. ARA of the obtained samples of essential oil surpasses the ARA values of aqueous, alcoholic and aqueous-alcoholic extracts. It was found that the APA value for 1 fraction of oil is 29.7% and increases with the transition to subsequent fractions to 54.3%. Whole Yarrow essential oil has an ARA value of 52.8%. According to the ARA value, the extractives of yarrow can be arranged in the following row: whole essential oils> 70% aqueous-alcoholic extract> 40% extract> 20% extract> aqueous extract> 96% alcoholic extract.

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ANTIRADICAL ACTIVITY OF THE ESSENTIAL OIL AND WATER-ALCOHOL EXTRACTS OF ORIGANUM VUL-GARE L., GROWING IN THE KRASNOYARSK TERRITORY

chemistry of plant raw material ◽

10.14258/jcprm.2021018785 ◽

2021 ◽

pp. 183-190

Author(s):

Irina Dement'yevna Zykova ◽

Aleksandr Alekseyevich Efremov

Keyword(s):

Essential Oil ◽

Antiradical Activity ◽

Water Extract ◽

Alcohol Concentration ◽

Origanum Vulgare ◽

Siberian Region ◽

Alcohol Extract ◽

Antiradical Properties ◽

Water Alcohol ◽

Model Reactions

The antiradical properties of essential oil and water-alcohol extracts of oregano (Origanum vulgare L.), which grows in the Krasnoyarsk territory, were studied in model reactions with a free stable 2,2-diphenyl-1-picrylhydrazyl radical. Essential oil is obtained by exhaustive hydro-distillation. Separate fractions of oil: first 10 minutes from the start of the distillation, the second – after 20 min, the third after 40 min, and the fourth in 80 minutes, a fifth fraction was collected after the end of hydroponically. The results of the DPPH test showed that all the studied samples of essential oil exhibit anti-radical activity (ARA), the values of which increase from 30.4% (the first fraction) to 51.0% (the fifth). The ARA of O. vulgare whole oil was 36.1%. The ARA values of O. vulgare water-alcohol extracts vary from 56.6 to 100% depending on the alcohol concentration and the volume of the added extract. The highest anti-radical activity is 70% extract, and the lowest – 96%. According to the ARA value, the studied extracts and essential oil are O. vulgare, which grows in the Siberian region, can be arranged in the following order: 70% water-alcohol extract > 40% extract > 20% extract > water extract > 96% alcohol extract > essential oil.

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ANTIRADICAL ACTIVITY OF SEPARATE FRACTIONS OF ESSENTIAL OIL OF ACORUS CALAMUS ROOTS

chemistry of plant raw material ◽

10.14258/jcprm.2020026659 ◽

2020 ◽

pp. 73-78

Author(s):

Irina Dement'yevna Zykova ◽

Aleksandr Alekseyevich Efremov ◽

Liliya Viktorovna Naimushina

Keyword(s):

Essential Oil ◽

Antiradical Activity ◽

Component Composition ◽

Biologically Active ◽

Acorus Calamus ◽

Siberian Region ◽

Antiradical Properties ◽

Main Components ◽

First Time ◽

Content Of Oxygen

Essential oil from the roots of calamus marsh (Acorus calamus L) was isolated by the method of exhaustive hydroparodistillation, growing on the Krasnoyarsk territory. Separate fractions of essential oil were obtained depending on the duration of isolation. The component composition of the essential oil, the main components of which are acorenone, butyrate α-terpineol, germacrene B, camphor. The antiradical properties of the essential oil of A. сalamus roots growing in the Siberian region, as well as the antiradical properties of its individual fractions were studied for the first time in order to determine the most promising samples as sources of biologically active complexes. To determine the antiradical activity, the reaction of the essential oil components with a stable free 2,2-diphenyl-1-picrylhydrazyl radical was used. The antiradical activity of all studied samples of essential oils was established. There was an increase in antiradical activity with a decrease in the content of monoterpenes in the composition of the essential oil. The lowest antiradical activity (17.5%) was shown by the fraction of essential oil with a high content of monoterpenes, the highest (~100%) – fractions with a high content of oxygen-containing compounds. It was revealed that the ability of components of individual fractions of A. сalamus essential oil to inhibit DPPH radicals is comparable with the antiradical activity of ascorbic acid solutions of equivalent concentration.

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ANTIRADICAL ACTIVITY OF ESSENTIAL OILS OF FILIPENDULA ULMARIA (L.) MAXIM, HYPERICUM PERFORA-TUM L. AND PULMONARIA MOLLIS WULFEN EX HORNEM OF THE KRASNOYRSK TERRITORI FLORA

chemistry of plant raw material ◽

10.14258/jcprm.2021039195 ◽

2021 ◽

pp. 211-217

Author(s):

Irina Dement'yevna Zykova ◽

Aleksandr Alekseyevich Efremov

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Antiradical Activity ◽

Component Composition ◽

Salicylic Aldehyde ◽

Filipendula Ulmaria ◽

Antiradical Properties ◽

Hypericum Perforatum L ◽

Butyl Phthalate ◽

Main Components

The antiradical properties of essential oils from the inflorescences of Filipendula ulmaria (L). Maxim, herbages Hypericum perforatum L. and Pulmonaria mollis Wulfen ex HORNEM., growing on the territory of the Krasnoyarsk territory were studied. For this purpose, the reaction of essential oil components with a stable free 2,2-diphenyl-1-picrylhydrazyl radical was used. Essential oil of the plants under study received comprehensive hydroponically. The component composition of the oils was determined by chromatography-mass spectrometry. The main components of essential oil of F. ulmaria inflorescences are methyl salicylate (28.2%), salicylic aldehyde (2.8 %) and linalool (4.9%), essential oil of H. perforatum – γ - amorphene (30.7%), δ-cadinen (7.1%), (E, E)-β-farnesene (5.5%), caryophyllene (5.0%), ledol (5.0%), essential oil of P. mollis – di-n-butyl phthalate (18.7%), docosan (13.4%), tetracosan (11.6 %). The results of the DPPH test showed that the essential oils of the inflorescences of F. ulmaria and the aboveground part of H. perforatum and P. mollis exhibit antiradical activity (ARA). According to the size of the ARA of essential oils, the studied plants can be arranged in the following row: P. mollis > F. ulmaria > H. perforatum.

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Essential oil content, chamazulene content and antioxidative properties of Achillea millefolium agg. extracts from Slovenia

Acta agriculturae Slovenica ◽

10.14720/aas.2021.117.2.2072 ◽

2021 ◽

Vol 117 (2) ◽

pp. 1

Author(s):

Boris TURK ◽

Dea BARIČEVIČ ◽

Franc BATIČ

Keyword(s):

Essential Oil ◽

Dry Matter ◽

Oil Content ◽

Steam Distillation ◽

Biochemical Properties ◽

Achillea Millefolium ◽

Antioxidative Capacity ◽

Distillation Method ◽

Significant Difference ◽

Diploid Taxon

The study aimed to clarify some biochemical properties, important for the phytopharmaceutical use of yarrow from the A. millefolium agg.. The study comprised 41 populations from Slovenia. The most abundant taxa were included: Achillea millefolium L., A. roseoalba Ehrend., A. collina (Wirtg.) Becker ex Rchb., A. distans Waldst. & Kit. ex Willd., A. pannonica Scheele, A. pratensis Saukel & R.Länger and A. nobilis L. Assessment of essential oil content with the steam distillation method showed no significant difference between taxa. Essential oil content was the lowest in A. collina (6.50 ml kg-1 of dry matter), followed by A. pannonica (7.75 ml kg-1), A. distans (8.50 ml kg-1), A. nobilis (9.40 ml kg-1), A. pratensis (9.65 ml kg-1), A. nobilis × A. millefolium (12.25 ml kg-1), A. roseoalba (12.75 ml kg-1) and A. millefolium (13.50 ml kg-1). The content of azulenes was determined by photometrical measurement of chamazulene in essential oil extracts. Chamazulene was only present in the diploid taxon and one tetraploid taxon, i.e., A. roseoalba (0.16 % of dry plant mass) and A. collina (0.05 %). The differences in antioxidative capacity of extracts from different taxa were not statistically significant, so we can assume that specific antioxidative capacity is not bound to a specific taxon or ploidy level.

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Anti-Neuropathic Pain Activity of Ageratum conyzoides L due to The Essential Oil Components

CNS & Neurological Disorders - Drug Targets ◽

10.2174/1871527319666201120144228 ◽

2020 ◽

Vol 19 ◽

Author(s):

Yedy Purwandi Sukmawan ◽

Kusnandar Anggadiredja ◽

I Ketut Adnyana

Keyword(s):

Neuropathic Pain ◽

Essential Oil ◽

Opioid Receptor ◽

Steam Distillation ◽

Chronic Constriction Injury ◽

Thermal Hyperalgesia ◽

Ageratum Conyzoides ◽

Safety And Efficacy ◽

Activity Test ◽

Oil Components

Background: Neuropathic pain is one of the contributors to the global burdens of illness. At present many patients do not achieve satisfactory pain relief even with synthetic pain-killers. Taking this into consideration, it is necessary to search for natural product-derived alternative treatment with confirmed safety and efficacy. Ageratum conyzoides L is a plant often used as analgesic in Indonesia, however, anti-neuropathic pain activity of this plant is still unknown. Objective: To determine the anti-neuropathic pain activity of the essential oil and non-essential oil component (distillation residue) of A. conyzoides L. Methods: We conducted separation of the essential oil component from other secondary metabolites through steam distillation. Both components were tested for anti-neuropathic pain activity using chronic constriction injury animal models with thermal hyperalgesia and allodynia tests. The animals were divided into 7 test groups namely normal, sham, negative, positive (pregabalin at 0.195 mg/20 g BW of mice), essential oil component (100 mg/kg BW), and non-essential oil component (100 mg/kg BW). Naloxone was tested against the most potent anti-neuropathic pain component (essential oil or nonessential oil) to investigate the involvement of opioid receptor. Results: The GC-MS of the essential oil component indicated the presence of 60 compounds. Meanwhile, non-essential oil components contained alkaloid, flavonoid, polyphenol, quinone, steroid, and triterpenoid. This non-essential oil component contained a total flavonoid equivalent to 248.89 ppm quercetin. The anti-neuropathic pain activity test showed significantly higher activity of the essential oil component compared to the non-essential oil component and negative groups (p<0.05). Furthermore, the essential oil component showed equal activity to pregabalin (p>0.05). However, this activity was abolished by naloxone, indicating the involvement of opioid receptor in the action of the essential oil component. Conclusion: The essential oil component of A. conyzoides L is a potential novel substance for use as anti-neuropathic pain.

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Innovation in a Continuous System of Distillation by Steam to Obtain Essential Oil from Persian Lime Juice (Citrus latifolia Tanaka)

Molecules ◽

10.3390/molecules26144172 ◽

2021 ◽

Vol 26 (14) ◽

pp. 4172

Author(s):

José Daniel Padilla-de la Rosa ◽

Magaly Dyanira Manzano-Alfaro ◽

Jaime Rosalío Gómez-Huerta ◽

Enrique Arriola-Guevara ◽

Guadalupe Guatemala-Morales ◽

...

Keyword(s):

Essential Oil ◽

Oil Recovery ◽

Steam Distillation ◽

Optical Rotation ◽

Process Time ◽

Chemical Compositions ◽

Distillation Process ◽

Steam Consumption ◽

Lime Juice ◽

Oil Fractions

The citrus industry is one of the most important economic areas within the global agricultural sector. Persian lime is commonly used to produce lime juice and essential oil, which are usually obtained by batch distillation. The aim of this work was to validate a patented continuous steam distillation process and to both physically and chemically characterize the volatile fractions of essential Persian lime oil. Prior to distillation, lime juice was obtained by pressing the lime fruit. Afterwards, the juice was subjected to a continuous steam distillation process by varying the ratio of distillate flow to feed flow (0.2, 0.4, and 0.6). The distillate oil fractions were characterized by measuring their density, optical rotation, and refractive index. Gas chromatography GC-FID was used to analyze the chemical compositions of the oil fractions. The process of continuous steam distillation presented high oil recovery efficiencies (up to 90%) and lower steam consumption compared to traditional batch process distillation since steam consumption ranged from 32 to 60% for different steam levels. Moreover, a reduction in process time was observed (from 8 to 4 h). The oil fractions obtained via continuous steam distillation differed significantly in their composition from the parent compounds and the fractions.

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