scholarly journals STUDY OF THE FLAVONOID COMPOSITION AND BIOLOGICAL ACTIVITY OF HAWTHORN`S LEAVES CRATAEGUS PONTICA

2021 ◽  
pp. 201-209
Author(s):  
Kamola Vakhabjanova Raimova ◽  
Nodira Gulomzhanovna Abdulladzhanova ◽  
Farrukh Nazimovich Toshpulatov ◽  
Nurali Azamovich Ergashev ◽  
Alimzhan Davlatbaevich Matchanov

The results of studies of the content of polyphenolic compounds and their antioxidant activity in the Pontic hawthorn plant Crataegus pontica K.Koch., growing in the mountainous regions of the Tashkent region of the Republic of Uzbekistan are presented. The collection of raw materials took place in the spring at the beginning of October 2019 and at the end of April 2020. The conditions for the isolation of polyphenolic compounds were selected under various conditions. It was shown that the optimal content of polyphenols is extracted with 70% acetone, followed by fractionation of the aqueous residue with ethyl acetate and precipitation with hexane. It was shown that in the plants harvested in spring, the amount of polyphenols was 4.28%, and the collected volume was 2.6%, of the air-dry mass of the raw material. Chromatographic methods (BC and TLC) revealed that the composition of plant leaves collected in spring contains more polyphenols than those collected in autumn. This plant contains compounds belonging to the class of flavonols, phenolic acids and flavan-3-ols. Polyphenols were identified by rutin, quercetin, quercetin-3-O-β-galactoside, catechin, and gallic acid by BC and TLC methods. The antioxidant activity of the sum of polyphenolic compounds was studied in the model of lipid peroxidation (LPO) in rat liver mitochondria. It was found that polyphenols have a protective effect on mitochondria, a reduced damaging effect of Fe2+ / ascorbate and antioxidant activity depends on the concentration of the studied polyphenolic substances. The introduction of rutin into the incubation medium in 5 μM medium inhibits LPO processes by 32.0%, and at 10 μM – by 85.9% and in 20 μM – by 96.8%, compared with the control.

2021 ◽  
pp. 96-99
Author(s):  
Алена Владимировна Сумина ◽  
Вадим Игоревич Полонский ◽  
Татьяна Михайловна Шалдаева

Одним из путей повышения функциональной ценности пищевых продуктов является увеличение содержания в них химических соединений, обладающих антиоксидантной активностью. Последние, как известно, играют важную роль в профилактике различных заболеваний человека. Антиоксидантами богаты зерновые культуры, на основе которых изготавливаются наиболее употребляемые населением продукты питания. Целью данного исследования являлось определение величины суммарного содержания антиоксидантов (ССА) в зерновом сырье на разных этапах производства хакасского национального продукта талган, изготовленного из пророщенного зерна овса. Овес выращивали на территории Бейского района Республики Хакасия в 2019 г. Очищенное зерно проращивали при комнатной температуре в течение 56 ч, затем его просушивали и обжаривали при температуре 180…200 °С в течение 10-15 мин, а далее измельчали и просеивали через сита с ячеей 0,9 мм. В результате получали продукт талган и отходы в виде отрубей. Для определения уровня ССА в зерне использовали два растворителя - горячую бидистиллированную воду и 70 %-ный этиловый спирт. Измерение значения ССА выполняли на приборе «Цвет Яуза-01-АА». Среди исследуемых образцов более высокие показатели ССА были зарегистрированы при использовании в качестве элюента горячей бидистиллированной воды. В работе выявлен эффект увеличения уровня ССА в конечном продукте по сравнению с исходным сырьем после этапа проращивания зерна в среднем в 2 раза, а после этапов обжаривания и измельчения в 2,1 раза. Величина ССА в отрубях превышала таковую в исходном зерне в среднем на 47 %. Таким образом, в результате исследований было установлено, что введение в технологию изготовления овсяного талгана дополнительного этапа, заключающегося в проращивании зерна, является одним из эффективных способов повышения величины ССА в полученном на его основе продукте. One of the ways to increase the functional value of food products is to increase the content of chemical compounds that have antioxidant activity. The latter are known to play an important role in the prevention of various human diseases. Antioxidants are rich in cereals, on the basis of which the most commonly consumed food products are made. The purpose of this study was to determine the value of the total content of antioxidants (TCA) in grain raw materials at different stages of production of the Khakass national product talgan, made from sprouted oat grain. Oats were grown on the territory of the Bey district of the Republic of Khakassia in 2019. The purified grain was sprouted at room temperature for 56 hours, then it was dried and fried at a temperature of 180…200 oC for 10-15 minutes, and then ground and sieved through sieves with a mesh of 0.9 mm. As a result, the product talgan and waste in the form of bran were obtained. To determine the level of TCA in the grain, two solvents were used - hot bidistilled water and 70 % ethyl alcohol. The TCA value was measured on the device «Color Yauza-01-AA». Among the studied samples, higher TCA values were recorded when using hot bidistilled water as an eluent. The paper reveals the effect of increasing the level of TCA in the final product in comparison with the initial raw material after the stage of grain germination by an average of 2 times, and after the stages of roasting and grinding by 2.1 times. The value of TCA in bran exceeded that in the original grain by an average of 47 %. Thus, as a result of research, it was found that the introduction of an additional stage in the production technology of oatmeal talgan, which consists in the germination of grain, is one of the effective ways to increase the value of TCA in the product obtained on its basis.


2020 ◽  
Vol 21 (4) ◽  
Author(s):  
Marcin Szymański ◽  
Daria Szajkowska ◽  
Arkadiusz Szymański

Introduction. All over the world, natural medicine uses species of the genus Melilotus due to their anti-inflammatory, anti-rheumatic, analgesic, spasmolytic, astringent, diuretic and anticoagulant effects. In the Polish Pharmacopoeia, only the yellow melilot herb (Meliloti herba) has a monograph. Aim. The research was aimed at a comparative analysis of the content of the sum of polyphenolic compounds, including the sum of flavonoids and phenolic acids, and antioxidant activity in water extracts of M. officinalis and M. alba herb and root. Material and methods. The research was carried out for two species of Melilotus officinalis and M. alba. The raw material was herb and melilot root, collected during flowering period from natural sites. In aqueous extracts from raw materials, the following methods were determined by colorimetric methods: the sum of polyphenolic compounds using the Folin-Ciocalteu reagent, the sum of phenolic acids using the Arnov reagent, the sum of flavonoids using the Christ-Müller method and the antioxidant activity using the 2,2-diphenyl-1-picryl-radical hydrazyl (DPPH). Results. Meliloti alba herb has a much higher content of polyphenols, including phenolic acids and flavonoids than M. officinalis. In the yellow sweet clover the sum of polyphenols in the samples ranged from 2.101 to 2.438%, while in the white sweet clover from 2.765 to 3.540%. The content of phenolic acids in the samples of yellow sweet clover herb was 0.617-0.766%, and in the samples of white sweet clover herb was 0.646-0.900%. The content of flavonoids in the samples of yellow sweet clover herb ranged from 0.748 to 0.975%, and in the case of white sweet clover herb it was at the level of 0.801-1.192%. The samples of the commercial yellow sweet clover herb had the lowest content of phenolic compounds; the sum of polyphenols was 1.579%, phenolic acids 0.361%, and flavonoids 0.441%. The determined content of total polyphenols in the roots of both melilot species was low and amounted to 0.450% in MORA1 and 0.362% in MARA2. Despite the lower content of polyphenolic compounds (2.438%), the yellow melilot showed higher antioxidant activity (IC50 = 4.35 mg/ml) compared to the white melilot herb (IC50 = 5.31) with a higher content of polyphenols (3.010%). Conclusions. A higher content of the sum of polyphenols as well as phenolic acids and flavonoids was found in the white melilot. In both the yellow and white sweet clover herb extract, significantly more polyphenolic compounds were determined compared to the content in their root extract.


2015 ◽  
Vol 1 (12) ◽  
Author(s):  
Slobodan Majstorović ◽  
Vladimir Malbašić ◽  
Miodrag Čelebić

Curent situation of raw material base and study of limestone like construction stone deposits in the Republic of Serbian were relatively poorly considered during the last decades, and the main problems are the following: low production capacity fragmentation of production, needs for raw materials homogenization and delivering of standard quality, undeveloped market, the recession of the domestic economy and the economies in the region, the relatively low level of exploration by many deposits, especially lack of knowledge of the occurrence legality for certain types and quality of raw materials and lack of complete quality indicators for raw material, which sometimes causes the utilization of only the highest quality deposit parts.This paper provides an overview of active quarries in the Republic Srpska with exploitation of limestone like technical building stone and present an attempt to determine the basic quantitative and qualitative characteristics of the raw material and the current working conditions at these quarries. The purpose of such a review would be in an effort to create a clearer picture about production, market, social and other aspects of the limestone exploitation in the Republika Srpska and the realistic possibilities of maintaining and developing of these mineral resources utilization, which can certainlybe interesting for the development of many other industries in the Republic Srpska.


Author(s):  
В. В. Поздняков ◽  
Ю. В. Харченко ◽  
Л. Я. Харченко ◽  
О. В. Анцыферова

Гибриды сахарной и сверхсахарной кукурузы являются хорошим сырьем для производства функциональных пищевых продуктов, в том числе диетического и детского питания. Важными показателями высокого качества этих продуктов являются повышенное содержание белка и ценного масла, незначительное количество плохо усвояемого кукурузного крахмала и высокое содержание антиоксидантов, а также отменные вкусовые качества. В работе представлены данные по оценке антиоксидантной активности большой группы новых перспективных гибридов сверхсахарной кукурузы среднеспелой группы, созданных с целью получения ценных источников сырья для консервной промышленности. Значения показателя общей антиоксидантной активности варьировали в широком интервале значений (от 31,8 % до 60,4 %, 568,4–1008 мкг/г семян), что указывает на перспективность использования этого важного биохимического параметра в селекции сверхсахарной кукурузы на качество. Hybrids of excess sugar maize are good raw material for the production of functional food products, including baby food and diet. Important indicators of high quality of these products are high protein content and a valuable oil, a small amount of poorly digestible maize starch and a high content of antioxidants, and also excellent taste qualities. The paper presents data on the evaluation of the antioxidant activity of a large group of promising new hybrids of excess sugar maize of mid-group created in order to obtain valuable raw materials for the canning industry. Index value of total antioxidant activity varied over a wide range (from 31,8 % to 60,4 %, 568,4–1,008 mg/g of seed), which indicates on promising use of this important parameter in the breeding of biochemical excess sugar maize on quality.


2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Laura Rimkiene ◽  
Asta Kubiliene ◽  
Andrejus Zevzikovas ◽  
Daiva Kazlauskiene ◽  
Valdas Jakstas

The aim of our research is to investigate the qualitative and quantitative composition and antioxidant activity of flavonoids in leaf samples ofGinkgo bilobaL.(G. biloba)growing in Lithuania and also to provide practical recommendations for the pharmaceutical industry to determine the optimum harvesting time and growth location ofG. bilobaleaves. The obtained results indicate that the growth location and time of sample collection have a significant influence (p≤0.05) on the content of active compounds. July and August are the best months to harvestG. bilobaleaves for industry. To ensure the rational collection ofG. bilobaraw material, the location of plantations is recommended in the western and southwestern phytoregions. The obtained results also indicate that the flavonoid content inG. bilobaleaf has a significantly positive correlation (r=0.952,p≤0.001) with antioxidant activity. The aqueous acetonic extracts from green leaves scavenge radicals to a greater degree than those from yellowGinkgoleaves. The results of this investigation provide information regarding the most suitable Lithuanian growing location to produceG. bilobawith an optimized content of health-promoting compounds.


2021 ◽  
Vol 1 (2) ◽  
pp. 1-11
Author(s):  
Ririt Yuniar

Generasi nasionalis, mengutamakan kepentingan bangsa Negara di atas kepentingan pribadi dan golongan. Artinya generasi yang taat hukum; disiplin; cinta tanah air; menghormati keragaman suku, agama, budaya; rela berkorban; mampu mengapresiasi budaya bangsa sendiri; menjaga kekayaan budaya bangsa; unggul dan berprestasi; serta mampu menjaga lingkungan. Sekolah, masyarakat, dan keluarga menjadi ekosistem pendidikan yang harus bersinergi. Terciptan karya seni kreatif dan inovatif yang memuat kelima sila beserta butir-butir Pancasila sebagai sebuah nilai luhur, keberagaman dan Kebhinekaan di Indonesia menjadi salah satu solusi alternatif bagi terwujudnya Negara Kesatuan Republik Indonesia (NKRI). Hal ini sejalan dengan Visi Misi ISI Yogyakarta, yaitu adanya penerapan dan pengoptimalan nilai-nilai Pancasila dalam konsep dan karyanya dengan memegang teguh konsep ideal bangsa Indonesia guna mewujudkan penciptaan seni maupun mengkaderisasi pendidik seni secara efektif juga menjadi tujuan utama tulisan ini. Teori strategi komunikasi (mengenal khalayak, merancang pesan, menetapkan metode, dan proses seleksi dalam penggunaan media) sangat relevan jika diterapkan oleh kreator seni dengan menggunakan pendekatan performance studies sebagai sebuah metode guna meningkatkan kualitas dan kreativitas para kreator seni yang lebih mengutamakan nilai-nilai Pancasila sebagai pedoman berkarya. Nilai-nilai Pancasila diimplementasikan melalui karya seni yang “borderless” artinya tidak terbatas lintas ruang dan waktu dalam konsep dan praktiknya. How to karya itu diciptakan, diproduksi, ditampilkan, dilestarikan, oleh para seniman, serta penikmat seni lainnya. Melalui pendidikan karakter yang berintegritas karya seringkali memuat substansi pesan moral di dalamnya. Nilai-nilai Pancasila raw-material yang fundamental dan terinternalisasi dalam kehidupan keseharian melalui berbagai profesi. The nationalist generation prioritizes the interests of the nation and state above personal and group interests. It means the law-abiding generation; discipline; love for the homeland; respecting ethnic, religious, cultural diversity; willing to sacrifice; able to appreciate the nation's culture; maintain the nation's cultural wealth; excel and achieve; and able to protect the environment. Schools, communities, and families become educational ecosystems that must work together. The creation of creative and innovative works of art containing the five precepts and the points of Pancasila as a noble value, diversity and diversity in Indonesia is one of the alternative solutions for realizing the Unitary State of the Republic of Indonesia (NKRI). This is in line with the Vision and Mission of ISI Yogyakarta, namely the application and optimization of Pancasila values in their concepts and works by upholding the ideal concept of the Indonesian nation in order to realize the creation of art well as to regenerate art educators effectively. The theory of communication strategy (knowing the audience, designing messages, determining methods, and selection processes in media used) is very relevant if applied by art creators using a performance studies approach as a method. This approach is to improve the quality and creativity of art creators who prioritize the values of Pancasila as a working guide. Pancasila values are implemented through borderless artwork, meaning they are not limited across space and time in concept and practice. How to work it was created, produced, displayed, preserved by artists and other art connoisseurs. Through character education with integrity, the work often contains the substance of the moral message in it. The values of Pancasila are raw materials that are fundamental and internalized in daily life through various professions. 


Author(s):  
T. V. Oproshanska ◽  
O. P. Khvorost

The aim of the work is to experimentally determine the optimal parameters for obtaining a tincture of rhizomes with roots of Sanguisorba officinalis and to investigate its antimicrobial and antioxidant activity. Materials and methods. Rhizomes with roots of Sanguisorba officinalis were harvested in autumn 2019. When establishing the optimal parameters of the technology of obtaining tincture, the evaluation criterion was the number of the sum of polyphenols and the amount of hydroxycinnamic acids, which were determined by spectrophotometric method according to the methods of the State Pharmacopoeia of Ukraine 2.0. Antimicrobial activity was studied by agar diffusion and serial dilutions, antioxidant – in vitro using a stable radical 2,2-diphenyl-1-picrylhydrazyl (2,2-diphenyl-1-picrylhydrazyl – DPPH). Results. The optimal parameters for obtaining a tincture of rhizomes with roots of Sanguisorba officinalis was extraction method maceration at room temperature, raw material-finished product ratio 1:5, time 48 hours, extractant 50 % ethyl alcohol, the number of polyphenols, and the number of hydroxycinnamic acids in terms of dry raw materials not less than 4.0 % and 1.5 % respectively. The resulting tincture exhibits antimicrobial activity against microorganisms Bacillus subtilis ATCC 6633 (growth retardation 26.40 ± 1.04 mm), Staphylococcus aureus ATCC 25923 (growth retardation 24.60 ± 0.68 mm) and Pseudomonas aeruginosa ATCС 27853 (growth retardation 23.60 ± 0.68 mm) and at a dose of 0.02 ml showed antioxidant activity at the level of 70 %. Conclusions. The parameters of obtaining a tincture of rhizomes with roots of Sanguisorba officinalis were determined and its antimicrobial and antioxidant activity was studied.


2020 ◽  
Vol 50 (1) ◽  
pp. 70-78 ◽  
Author(s):  
Tatiana Tanashkina ◽  
Anastasia Peregoedova ◽  
Anna Semenyuta ◽  
Margarita Boyarova

Introduction. The production of gluten-free food products, including alcohol-free beverages, is an urgent task for Russian food industry. Buckwheat malt has a great raw material potential, because it consists rutin, which can give the final product some useful functional properties. Aromatic raw materials improve the sensory characteristics of beverages and increase their nutritional value. Study objects and methods. Kvass is a traditional Russian bread juice. To prepare gluten-free kvass, we used light and scalding buckwheat malt and water infusions of barberry, juniper, leaves of garden currant, and lemon balm grass. The wort was prepared by infusion and fermented with bottom beer yeast Saflager W-34/70. Then it was blended with infusions of plant materials. Sensory and physical and chemical properties of kvass were determined by standard methods. Antioxidant activity of the beverages was assessed by using DPPH radical scavenging method. Results and discussion. All samples met the state standard requirements for kvass by sensory, physical, and chemical characteristics. The drinks obtained from the scalding malt were well-fermented and achieved better tasting ratings compared to those from the light malt. The control sample, which contained no aromatic raw materials, received the lowest scores for taste and aroma. The kvass blended with infusions of barberry and garden currant had the best results. The infusions increased the shelf life of the kvass from 7 days (control) to 12 (barberry), 14 (juniper), and 16 (currant). All samples of kvass demonstrated antioxidant activity, the beverage with infusion of garden currant leaves showing the best results (≤ 80%). Antiradical activity was established for the samples blended with barberry and currant infusions. Conclusion. light and scalding buckwheat malt can be used to prepare gluten-free kvass. Blending buckwheat kvass with infusions of aromatic raw materials increased the shelf life of the beverages, improved their taste, aroma, and antioxidant activity (except the sample with infusions of juniper), and gave them antiradical properties.


Medicina ◽  
2009 ◽  
Vol 45 (5) ◽  
pp. 382 ◽  
Author(s):  
Raimondas Raudonis ◽  
Valdas Jakštas ◽  
Deividas Burdulis ◽  
Raimondas Benetis ◽  
Valdimaras Janulis

The most important attention is paid to the search of natural antioxidants and their evaluation in medicinal and food raw materials of plant origin. A number of plants, their extracts, food products, and medicinal preparations appear to be the objects of scientific research. Effectiveness and informative character of research, undoubtedly, depend on relevance, sensitivity, and efficiency of the methods chosen. The aim of this work was to develop and validate the postcolumn high-performance liquid chromatography (HPLC)-DPPH method as well as its application in the evaluation of antioxidant activity of known and unknown compounds scavenging free radicals and existing in medicinal plant raw materials. HPLC-separated compounds were identified at the wavelength of 275 nm, and then the mobile phase with analytes flowed through a mixing tee to the reaction coil, where DPPH reagent solution was supplied. The solution flow rate was 0.4 mL/min. The reaction coil was connected with UV/VIS type detector, which measured absorption of flowing solution at the wavelength of 520 nm. It was determined that vitexin rhamnoside, the dominant compound in the leaves of Crataegus monogyna, was not a significant radical scavenger. The most active antioxidant in the leaves and flowers of Crataegus monogyna was chlorogenic acid. The most active antioxidant in Origanum vulgare raw material was rosmarinic acid. Identified analytes in the extracts of Achillea millefolium that possessed radical-scavenging properties were chlorogenic acid, luteolin-7-Oglucoside, rutin, and luteolin.


Author(s):  
V. M. Shishmarev ◽  
◽  
T. M. Shishmareva ◽  

The coenopopulations of Serratula centauroides in various plant communities of Transbaikalia have been studied. In the study areas 10 geobotanical descriptions were made in plant communities with the participation of S. centauroides. In the investigated area S. centauroides is found on dry rocky slopes, in herb and sandy steppes. Depending on the growing conditions, the density of individuals in coenopopulations ranges from 0.18 to 1.80 individuals per 1 m2. The highest occurrence of S. centauroides is noted in communities dominated by Gramineous and Artemisia: Gramineous-Artemisia-herb, Filifolium-Artemisia-Gramineous, Gramineous-Artemisia, Oxytropis-Gramineous and Allium-Artemisia. Studied coenopopulations S. centauroides are confined to the following communities: Filifolium-herb, Gramineous- Artemisia-herb, Sophora-herb, Filifolium-Artemisia-Gramineous, Gramineous-Artemisia, Agropyron- Carex, Carex-herb, Oxytropis-Gramineous and Allium-Artemisia. The phytomass, biological and exploitation resources, the possible volumes of annual harvesting of raw material of S. centauroides were determined. The phytomass of S. centauroides herb ranges from 8.10±3.46 to 75.93±19.55 g/m2 (air-dry raw material). The average phytomass of 1 individual of S. centauroides herb varies from 13.67±3.59 to 67.14±14.30 g (air-dry raw materials). The total biological resources of S. centauroides herb is 777.9 t (fresh raw materials) and 266.8 tons (air-dry raw materials). The total exploitation resources of S. centauroides herb is 224.7 t (fresh raw materials) and 79.8 t (air-dry raw materials). The possible volume of annual harvesting of raw materials of S. centauroides in the studied coenopopulations on the territory of the Trans-Baikal Territory and the Republic of Buryatia amounts to 37.5 t (fresh raw materials) and 13.3 t (air-dry raw materials).


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