scholarly journals Parameters of obtaining tincture from underground organs of Sanguisorba officinalisand study its biological activity

2021 ◽  
Vol 14 (3) ◽  
pp. 299-305
Author(s):  
T. V. Oproshanska ◽  
O. P. Khvorost
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Growth Retardation ◽  
Raw Materials ◽  
Hydroxycinnamic Acids ◽  
Raw Material ◽  
Optimal Parameters ◽  
Antimicrobial And Antioxidant Activity ◽  
Sanguisorba Officinalis

The aim of the work is to experimentally determine the optimal parameters for obtaining a tincture of rhizomes with roots of Sanguisorba officinalis and to investigate its antimicrobial and antioxidant activity. Materials and methods. Rhizomes with roots of Sanguisorba officinalis were harvested in autumn 2019. When establishing the optimal parameters of the technology of obtaining tincture, the evaluation criterion was the number of the sum of polyphenols and the amount of hydroxycinnamic acids, which were determined by spectrophotometric method according to the methods of the State Pharmacopoeia of Ukraine 2.0. Antimicrobial activity was studied by agar diffusion and serial dilutions, antioxidant – in vitro using a stable radical 2,2-diphenyl-1-picrylhydrazyl (2,2-diphenyl-1-picrylhydrazyl – DPPH). Results. The optimal parameters for obtaining a tincture of rhizomes with roots of Sanguisorba officinalis was extraction method maceration at room temperature, raw material-finished product ratio 1:5, time 48 hours, extractant 50 % ethyl alcohol, the number of polyphenols, and the number of hydroxycinnamic acids in terms of dry raw materials not less than 4.0 % and 1.5 % respectively. The resulting tincture exhibits antimicrobial activity against microorganisms Bacillus subtilis ATCC 6633 (growth retardation 26.40 ± 1.04 mm), Staphylococcus aureus ATCC 25923 (growth retardation 24.60 ± 0.68 mm) and Pseudomonas aeruginosa ATCС 27853 (growth retardation 23.60 ± 0.68 mm) and at a dose of 0.02 ml showed antioxidant activity at the level of 70 %. Conclusions. The parameters of obtaining a tincture of rhizomes with roots of Sanguisorba officinalis were determined and its antimicrobial and antioxidant activity was studied.

scholarly journals THE IMPACT OF PLANT MATERIAL FREEZING AND EXTRACTANT ACIDIFYING ON THE ANTIOXIDANT POTENTIAL AND PIGMENTS CONTENT IN EXTRACTS AND RAW MATERIALS OF DIFFERENT CARROT VARIETIES

2020 ◽  
Vol 20 (2) ◽  
pp. 33-42
Author(s):  
Anna Muzykiewicz-Szymańska ◽  
Joanna Zielonka-Brzezicka ◽  
Adam Klimowicz
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Raw Materials ◽  
Raw Material ◽  
Extraction Time ◽  
Antioxidant Potential ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
The Impact

The antioxidant activities of extracts of purple, yellow and orange carrot varieties were evaluated by in vitro methods i.e. DPPH, ABTS, FRAP, and CUPRAC. The total polyphenols concentration was evaluated using Folin-Ciocalteu technique. The content of anthocyanins, chlorophylls and carotenoids was evaluated in fresh and frozen carrots. The anthocyanin concentration was also determined in carrot extracts. The effect of certain parameters of ultrasound-assisted extraction, i.e. extraction time, concentration and acidifying of extractant, as well as the type of raw material (fresh or frozen) was analyzed. The antioxidant potential as well as content of polyphenols and plant pigments were the highest in purple carrot extracts. The most effective extractant seems to be 70% (v/v) ethanol. The extension of the extraction time, in majority of cases, led to an increase of antioxidant activity of extracts, whereas the freezing of raw material generally decreased the above-mentioned activities. The effect of acidification of extractant is ambiguous and depends on the method applied to evaluate antioxidant activity.

Synthesis, Characterization and Evaluation of Antioxidant and Antimicrobial activity of Spirochromones Derivatives

2021 ◽  
Vol 18 (4) ◽  
pp. 352-361
Author(s):  
Achut R. Shinde ◽  
Dyanoba B. Muley
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Pyroglutamic Acid ◽  
Antioxidant And Antimicrobial Activity ◽  
Antimicrobial And Antioxidant Activity

Background: Plethora of literature reported hitherto, depicts impending biological activities for spirochromones. This fact encouraged us for the exploration of the synthesis of new substituted spirochromones and their evaluation of their bioactivities. Objective: To synthesize different substituted spirochromones derivatives from a spirochromones salt using (R)-pyroglutamic acid as catalyst and their evaluation for antimicrobial and antioxidant activities. Methods: Herein, Aldol cascade method was used for the syntheses of spirochromone derivatives from its salt. A mild protocol is developed for the syntheses of spirochromones derivatives by using (R)-pyroglutamic acid as catalyst. Further, the derivatives and its precursor salt were screened for antimicrobial and antioxidant activity. The antioxidant potentials of the synthesized compounds were checked by DPPH and hydroxyl free radical scavenging assay. Consequently, in vitro antimicrobial activities were performed by well diffusion assay against gram negative bacterium (E. coli) and gram positive bacterium (S. aureus). Results: In the present work, 10 synthesized derivatives are obtained in harmonious yield from a precursor spirochromones salt using (R)-pyroglutamic acid as catalyst. Amongst the studied compounds, 6a depicted maximum antimicrobial activity against the screened microorganisms along with maximum antioxidant activity. Albeit, the synthesized motifs showed moderate to good antioxidant and antimicrobial activities. Conclusion: A mild protocol was developed for the synthesis of spirochromone derivatives from their precursor spirochromones salt by using (R)-pyroglutamic acid as catalyst, which showed moderate to good antimicrobial and antioxidant activity.

SYNTHESIS OF NOVEL BENZOTRIAZOLYL FORMAZANS AND THEIR ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (11) ◽  
pp. 29-33
Author(s):  
S George ◽  
◽  
R. Chakraborty ◽  
F. Saleshier ◽  
T.K. Ravi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Diazonium Salt ◽  
Aromatic Aldehydes ◽  
Bacterial Strains ◽  
Mass Spectral ◽  
Antimicrobial And Antioxidant Activity

Benzotriazolyl hydrazide (2) has been obtained from the ester ethyl benzotriazolyl acetate (1). The hydrazide with various aromatic aldehydes in presence of glacial acetic acid gave corresponding arylidenes 3(a-h). The arylidenes were made to react with fluoro substituted diazonium salt whereby, benzotriazolyl formazans 4(a-h) were obtained. The structures of the compounds were confirmed by FTIR, 1 H-NMR, mass spectral data and elemental analysis. All the synthesized compounds were screened for their in-vitro antimicrobial activity against the bacterial strains Bacillus subtilis and Escherichia coli and the fungal strains Asperigillus niger and Candida albicans by two fold serial dilution technique and also for their antioxidant activity. Among the compounds tested, compound 4b (fluoro substituted derivative) showed MIC as low as 15.6 µg/mL against Bacillus subtilis and Candida albicans. Other derivatives also showed moderate antimicrobial activity. Also, compound 4e (methoxy substituted derivative) showed significant antioxidant activity when compared to the standard, ascorbic acid.

Evaluation of antioxidant and antimicrobial activity of Ziziphora ‎clinopodiodes essential oil eluted at different times

2020 ◽  
Vol 16 ◽  
Author(s):  
Mojgan Alizadeh ◽  
Akram Arianfar ◽  
Ameneh Mohammadi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Positive Control ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Citronellic Acid ◽  
Antimicrobial And Antioxidant Activity

Objective: Ziziphora clinopodioides is an edible medicinal plant belongs to the Labiatae family that widespread all over Iran. It used as culinary and also in cold and cough treatments in Iran. The aim of present work was to evaluate the effect of different timeframes during the hydrodistillation on essential oil composition, antimicrobial and antioxidant activity. Materials and Methods: The essential oil of Z. clinopodiodes was extracted via hydrodistillation with Clevenger apparatus. The fractions of essential oil were captured at 6 times from the beginning of the distillation: (10, 20, 60, 120, 180 and 240 min). The fractions of essential oil were analyzed by GC/MS and their antibacterial, antifungal and antioxidant activities were studied by Disk - well diffusion and DPPH methods respectively. Results: Six distillation times and whole essential oil were captured during the hydrodistillation. Essential oil yield dropped off significantly during distillation progressed (1.0% for 10 min and 0.025 for 240 min). 1,8 Cineol, Isomenthone, Pulegone, Piperitenone and Citronellic acid were major compounds in fractions and they were affected by distillation times. Pulegone was major compound in all of essential oils. In antioxidant activity assay, whole essential oil was stronger than was stronger than positive control and fractions of essential oil, because of higher levels of Isomenthone, Piperitenone and Citronellic acid. Strongest antimicrobial activity against S. aureus, E. coli and C. albicans was observed from 10 min fraction. Conclusion: Our results indicated that distillation time can create essential oils with specific properties and we can achieve to more efficient essential oil in short times.

scholarly journals Microwave-Assisted Synthesis, Characterization, Antimicrobial and Antioxidant Activity of Some New Isatin Derivatives

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
G. Kiran ◽  
T. Maneshwar ◽  
Y. Rajeshwar ◽  
M. Sarangapani
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging ◽  
Microwave Assisted Synthesis ◽  
Stable Free Radical ◽  
Ft Ir ◽  
Antimicrobial And Antioxidant Activity ◽  
New Compounds ◽  
Isatin Derivatives

A series of β-Isatin aldehyde-N,N′-thiocarbohydrazone derivatives were synthesized and assayed for theirin vitroantimicrobial and antioxidant activity. The new compounds were characterized based on spectral (FT-IR, NMR, MS) analyses. All the test compounds possessed a broad spectrum of activity having MIC values rangeing from 12.5 to 400 μg/ml against the tested microorganisms. Among the compounds3e,3jand3nshow highest significant antimicrobial activity. The free radical scavenging effects of the test compounds against stable free radical DPPH (α,α-diphenyl-β-picryl hydrazyl) and H2O2were measured spectrophotometrically. Compounds3j,3n,3l, and3e, respectively, had the most effective antioxidant activity against DPPH and H2O2scavenging activity.

scholarly journals Phytochemical Fingerprinting and In Vitro Antimicrobial and Antioxidant Activity of the Aerial Parts of Thymus marschallianus Willd. and Thymus seravschanicus Klokov Growing Widely in Southern Kazakhstan

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3193
Author(s):  
Bagda Sagynaikyzy Zhumakanova ◽  
Izabela Korona-Głowniak ◽  
Krystyna Skalicka-Woźniak ◽  
Agnieszka Ludwiczuk ◽  
Tomasz Baj ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Protocatechuic Acid ◽  
Antioxidant Activities ◽  
Methyl Esters ◽  
Bactericidal Effect ◽  
Ethyl Esters ◽  
Volatile Fraction ◽  
Aerial Parts ◽  
Antimicrobial And Antioxidant Activity

The chemical composition of the hydroethanolic extracts (60% v/v) from the aerial parts of Thymus marschallianus Willd (TM) and Thymus seravschanicus Klokov (TS) from Southern Kazakhstan flora was analyzed together with their hexane fractions. Determination of antibacterial, antifungal and antioxidant activities of both extracts was also performed. RP-HPLC/PDA and HPLC/ESI-QTOF-MS showed that there were some differences between the composition of both extracts. The most characteristic components of TM were rosmarinic acid, protocatechuic acid, luteolin 7-O-glucoside, and apigenin 7-O-glucuronide, while protocatechuic acid, luteolin 7-O-glucoside, luteolin 7-O-glucuronide, and eriodictyol predominated in TS. The content of polyplenols was higher in TS than in TM. The GC-MS analysis of the volatile fraction of both examined extracts revealed the presence of thymol and carvacrol. Additionally, sesquiterpenoids, fatty acids, and their ethyl esters were found in TM, and fatty acid methyl esters in TS. The antioxidant activity of both extracts was similar. The antibacterial activity of TS extract was somewhat higher than TM, while antifungal activity was the same. TS extract was the most active against Helicobacter pylori ATCC 43504 with MIC (minimal inhibitory concentration) = 0.625 mg/mL, exerting a bactericidal effect. The obtained data provide novel information about the phytochemistry of both thyme species and suggest new potential application of TS as a source of bioactive compounds, especially with anti-H. pylori activity.

scholarly journals Antimicrobial Activity and Polyphenol Profiles of Hydroalcoholic Extracts of Thymus rasitatus Klokov and Thymus eremita Klokov

2021 ◽  
Vol 9 (A) ◽  
pp. 313-317
Author(s):  
Svetlana Ivasenko ◽  
Ainur Zhumabekova ◽  
Agnieszka Ludwiczuk ◽  
Krystyna Skalicka–Wozniak ◽  
Alexandr Marchenko ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chromatographic Analysis ◽  
Antimicrobial Agents ◽  
Raw Materials ◽  
Wide Spectrum ◽  
Raw Material ◽  
Thymus Vulgaris ◽  
Material Base ◽  
Ultrasound Assisted Extraction ◽  
Ethanol Extracts

BACKGROUND: A possible reduction in stocks of medicinal plant raw materials of Thymus serpyllum L. and Thymus vulgaris L. leads to the need to expand the raw material base of the official medicinal plants with using of endemic species of the flora of Kazakhstan, in particular, Thymus rasitatus Klokov, and Thymus eremita Klokov. AIM: The aim of the study was to study the possibility of using 70% ethanol extracts of T. rasitatus and T. eremita as antimicrobial agents. MATERIALS AND METHODS: The aerial parts of T. rasitatus and T. eremita were extracted with 70% ethanol using ultrasound assisted extraction. The qualitative and quantitative analyses of the 70% ethanol extracts were determined using the liquid chromatography-detection-ESI-mass spectrometry-(MS)/MS technique. The study of the antimicrobial activity of these extracts was performed for eight strains of Gram-positive bacteria, six strains of Gram-negative bacteria, and four cultures of fungi. RESULTS: Chromatographic analysis of hydroalcoholic extracts of both investigated Thymus species showed very similar phenolic compounds composition. In both cases, the major components are luteolin-7-O-glucoside and rosmarinic acid. About 70% ethanol extracts of T. rasitatus and T. eremita have a broad spectrum of antimicrobial activity, exhibit the bactericidal or bacteriostatic activity against all tested bacteria and fungi at concentration range of 0.0195–20 mg/ml, but differ in their potency against tested strains of microorganisms. CONCLUSION: About 70% ethanol extracts of T. rasitatus and T. eremita, endemic plants in the flora of Kazakhstan, can be considered as potential drugs with a wide spectrum of antimicrobial activity. The results of chromatographic analysis could be used for drug standardization.

scholarly journals In-vitro antimicrobial and antioxidant activity of Argyreia cuneata (Willd.) Ker Gawl. (Convolvulaceae)

2018 ◽  
Vol 8 (6) ◽  
pp. 22-27
Author(s):  
TR Prashith Kekuda ◽  
Nitish A. Bharadwaj ◽  
MB Sachin ◽  
BK Sahana ◽  
GS Priyanka
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Leaf Extract ◽  
Shigella Flexneri ◽  
Radical Scavenging ◽  
Flower Extract ◽  
Stem Extract ◽  
Poisoned Food Technique ◽  
Antimicrobial And Antioxidant Activity

Objectives: Argyreia cuneata (Willd.) Ker Gawl. belongs to the family Convolvulaceae. The present study was performed to screen the potential of crude extract of various parts of A. cuneata to exhibit antimicrobial activity. Methods: Extraction of shade dried and powdered leaf, stem and flower of A. cuneata was carried out by maceration technique. Antibacterial and antifungal activity of extracts was evaluated by Agar well diffusion and Poisoned food technique respectively. Antioxidant activity was determined by DPPH radical scavenging, ABTS radical scavenging and ferric reducing assays. Results:  All extracts were effective in inhibiting test bacteria and the susceptibility of bacteria to extracts was in the order: Bacillus cereus > Shigella flexneri > Escherichia coli > Salmonella typhimurium. Leaf extract and stem extract exhibited highest and least antibacterial activity, respectively. Extracts were effective in causing inhibition of seed-borne fungi viz. Aspergillus niger and Bipolaris sp to >50%. Leaf extract exhibited marked antifungal activity followed by flower extract and stem extract. All extracts were shown to exhibit concentration dependent scavenging and reducing activity. Antioxidant activity of extracts observed was in the order: leaf extract > flower extract > stem extract.  Conclusion: Among various parts of A. cuneata, leaf extract exhibited marked antimicrobial and antioxidant activity. The plant can be employed as an effective antimicrobial and antioxidant agent in suitable form. Further studies may be undertaken to recover phytochemicals from the plant and to investigate the antimicrobial and antioxidant activity of isolated components. Keywords: Argyreia cuneata, Maceration, Antimicrobial, Agar well diffusion, Poisoned food technique, Antioxidant

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