scholarly journals Comparative Evaluation of Extrusion of Apical Debris and Irrigant from Curved Root Canals Using Three Different Ni-Ti Instruments with Conventional Needle Irrigation Methods - An In Vitro Study

2021 ◽  
Vol 10 (34) ◽  
pp. 2920-2923
Author(s):  
Baljeet Singh Hora ◽  
Yadnesh Arun Dondulkar ◽  
Vaibhav Suresh Jain ◽  
Shailendra Kumar Sahu ◽  
Nitin Prakash Chand Jogad ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Experimental Models ◽  
Agar Gel ◽  
Root Canals ◽  
Irrigation Methods ◽  
Working Length ◽  
Significant Difference ◽  
Operative Outcome ◽  
Curved Root Canals

BACKGROUND Extrusion of debris, bacteria, and irrigant effect the inter-appointment flare ups and post-operative outcome of the endodontic treatment. So, it is necessary to make every effort to minimize such extrusion during cleaning and shaping of the canals. The present study was done to compare and evaluate debris and irrigant extrusion from curved root canals using different Ni-Ti systems. METHODS 30 mesial roots of mandibular molars were used in this study. Crown were decoronated, working length and initial apical diameter was established. 1.5 % agar gel model was used in this study. Samples were assigned randomly into 3 groups (n = 10 teeth per group). ProTaper Next, One Shape, FANTA AF BLUE F ONE files were used according to the manufacturer’s instructions for canal instrumentation. Apically extruded debris and irrigant was computed after the biomechanical preparation and their comparative analysis for each of the instruments and experimental models was performed. RESULTS Statistically significant difference was found between the three experimental groups. (P < .05). CONCLUSIONS All the instruments produced apically extruded debris and irrigant, but least was seen with FANTA AF BLUE F ONE and maximum with One Shape among the experimental groups. KEY WORDS Apical Debris, Irrigant, NiTi Files

scholarly journals The Antibacterial Efficacy of Biopure MTAD in Root Canal Contaminated with Enterococcus faecalis

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Blerim Kamberi ◽  
Donika Bajrami ◽  
Miranda Stavileci ◽  
Shuhreta Omeragiq ◽  
Fatmir Dragidella ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Sodium Hypochlorite Solution ◽  
Antibacterial Efficacy ◽  
Human Teeth ◽  
Exact Test ◽  
Hypochlorite Solution ◽  
Root Canals ◽  
Significant Difference ◽  
Level Of Significance

Aim. The purpose of this in vitro study was to assess the antimicrobial efficacy of Biopure MTAD against E. faecalis in contaminated root canals. Materials and Methods. Forty-two single rooted extracted human teeth were inoculated with E. faecalis and incubated for four weeks. The samples were divided in two control and five experimental groups irrigated with 1.5% sodium hypochlorite solution (NaOCl); 3% NaOCl; BioPure MTAD; 1.5% NaOCl/17% EDTA; or 3% NaOCl/17% EDTA. After a one-week incubation, complete disinfection was confirmed by the absence of turbidity in the incubation media. Dentin shavings were taken from samples with no turbidity to verify whether E. faecalis was present in dentin tubules. Results were analyzed statistically using Fisher's exact test, with the level of significance set at . Results. Statistical analysis of the data obtained at Day 7 and after dentin shaving analysis showed that BioPure MTAD had significantly greater antibacterial activity than 1.5% NaOCl, 1.5% NaOCl/17% EDTA and 3% NaOCl/17% EDTA. No significant difference was detected between MTAD and 3% NaOCl. Conclusions. These findings suggest that BioPure MTAD possesses superior bactericidal activity compared with NaOCl and EDTA against E. faecalis.

scholarly journals Effectiveness of hand and Mtwo™ instruments in preparing oval-shaped root canals: in vitro study

2016 ◽  
Vol 21 (2) ◽  
Author(s):  
Fernanda Diederichs Coutinho ◽  
Renata Dornelles Morgental ◽  
Simone Bonato Luisi ◽  
Fabiana Vieira Vier-Pelisser ◽  
Patricia Maria Poli Kopper
Keyword(s):  
Root Canal ◽  
In Vitro Study ◽  
Root Apex ◽  
Conventional Technique ◽  
Basic Sequence ◽  
Preparation Technique ◽  
Root Canals ◽  
Canal Area ◽  
Significant Difference

Objective: This study evaluated through stereomicroscopy the effectiveness of hand and rotary instrumentation techniques, either isolated or combined, in the preparation of oval-shaped root canals. Materials and method: Thirty single-rooted human mandibular incisors were selected. After endodontic access and coronal preflaring, teeth were mounted in a modified Bramante muffle and then sectioned transversely at 3 and 6 mm from the root apex. Images of each section were made under a stereomicroscope at 30× magnification. Teeth were reassembled in the muffle and divided into three groups (n=10) according to the root canal preparation technique: GI - rotary preparation with Mtwo™ basic sequence (10/.04; 15/.05; 20/.06; 25/.06), followed by Mtwo™ complementary instruments (30/.05; 35/.04; 40/.04); GII - rotary preparation with Mtwo™ basic sequence, complemented by hand instruments (#30, #35 and #40); GIII - hand instrumentation using the conventional technique (#10 to #40). All instruments were used in brushing motion. The muffles were separated again so that new images of each section could be obtained. The following parameters were evaluated in pre- and postoperative images: root canal area; perimeter; mesiodistal (MD) and buccolingual (BL) diameters; and mesial (M) and distal (D) wall thickness. Next, the values measured in post- and preoperative images were subtracted forcomparison of experimental groups. Data were analyzed using the Kruskal-Wallis test (α=0.05). Results: No significant difference between groups was observed for all parameters. Conclusion: There was no difference among hand, rotary, or combined instrumentation; all techniques were able to increase root canal area, perimeter, and diameter in MD and BL directions. Moreover, the amount of dentin wearon proximal root canal walls was similar for all tested groups.

Microbial Leakage of Cavit, IRM, and Temp Bond in Post-prepared Root Canals Using Two Methods of Gutta-percha Removal: An In Vitro Study

2005 ◽  
Vol 6 (3) ◽  
pp. 53-61 ◽  
Author(s):  
Hanan Balto ◽  
Saad Al-Nazhan ◽  
Khulood Al-Mansour ◽  
Moneera Al-Otaibi ◽  
Yunus Siddiqu
Keyword(s):  
Root Canal ◽  
Bacterial Suspension ◽  
Control Group ◽  
Endodontically Treated Teeth ◽  
Root Canals ◽  
Gutta Percha ◽  
Working Length ◽  
Significant Difference ◽  
Post Space

Abstract The aim of this study was to evaluate the integrity of the coronal seal of Temp-Bond and compare it to Cavit and IRM after post space preparation using S. faecalis as a microbial tracer. In addition, the affect of two methods of gutta percha removal on the apical seal of root canal fillings was also evaluated. Forty extracted human single rooted teeth were prepared chemomechanically and obturated with gutta percha and AH26 sealer cement using the lateral cold condensation technique to a standardized working length of 15 mm. About 10 mm of the coronal gutta-percha was removed with either Peeso-reamer or a hot plugger. The roots were divided into three experimental groups of 10 roots and a control group. Each experimental group was subdivided equally into two groups of 15 each according to the method of post space preparation. Cavit, IRM, and Temp-Bond were used to seal the access opening. Each root was fixed in a cuvette containing Tryptic Soya Broth which, covered 2 mm of the root apex. Bacterial suspension was introduced through pipette. Fresh bacterial suspension was added every week, and the system was monitored daily for the growth of microorganisms for a period of one month. The results showed there was no significant difference in terms of coronal leakage between the three coronal materials used (P=0.478), but the methods of gutta-percha removal did have an impact on the apical leakage (P=0.047). The mean value showed the Peeso-reamer provided less leakage compared to using a hot plugger during the 30-day experimental time period. It was concluded the temporary type of coronal seal of endodontically treated teeth will not prevent coronal leakage if left for a long period of time. In addition, permanent cementation of the post with the coronal restoration should be carried out as soon as possible to prevent recontamination of the root canal. Citation Balto H, Al-Nazhan S, Al-Mansour K, Al-Otaibi M, Siddiqu Y. Microbial Leakage of Cavit, IRM, and Temp Bond in Post-prepared Root Canals Using Two Methods of Gutta-percha Removal: An In Vitro Study. J Contemp Dent Pract 2005 August;(6)3:053-061.

scholarly journals [NO TITLE AVAILABLE]

2007 ◽  
Vol 18 (4) ◽  
pp. 289-293 ◽  
Author(s):  
Orlando Limongi ◽  
Diana Santana de Albuquerque ◽  
Flares Baratto Filho ◽  
José Roberto Vanni ◽  
Elias P. Motcy de Oliveira ◽  
...  
Keyword(s):  
Root Canal ◽  
Multislice Ct ◽  
In Vitro Study ◽  
Ct Scanner ◽  
Root Canals ◽  
Group I ◽  
Working Length ◽  
Root Dentin ◽  
Dentin Thickness

This in vitro study compared, using computed tomography (CT), the amount of dentin removed from root canal walls by manual and mechanical rotary instrumentation techniques. Forty mandibular incisors with dental crown and a single canal were selected. The teeth were randomly assigned to two groups, according to the technique used for root canal preparation: Group I - manual instrumentation with stainless steel files; Group II - mechanical instrumentation with RaCe rotary nickel-titanium instruments. In each tooth, root dentin thickness of the buccal, lingual, mesial and distal surfaces in the apical, middle and cervical thirds of the canal was measured (in mm) using a multislice CT scanner (Siemens Emotion, Duo). Data were stored in the SPSS v. 11.5 and SigmaPlot 2001 v. 7.101 softwares. After crown opening, working length was determined, root canals were instrumented and new CT scans were taken for assessment of root dentin thickness. Pre- and post-instrumentation data were compared and analyzed statistically by ANOVA and Tukey's post-hoc test for significant differences (p=0.05). Based on the findings of this study, it may be concluded that regarding dentin removal from root canal walls during instrumentation, neither of the techniques can be considered more effective than the other.

scholarly journals Apical Extrusion of Intracanal Debris Using Two Engine Driven and Step-Back Instrumentation Techniques: An In-Vitro Study

2008 ◽  
Vol 02 (04) ◽  
pp. 233-239 ◽  
Author(s):  
Alper Kustarci ◽  
Neslihan Akdemir ◽  
Seyda Herguner Siso ◽  
Demet Altunbas
Keyword(s):  
Stainless Steel ◽  
Analysis Of Variance ◽  
Statistical Significance ◽  
In Vitro Study ◽  
Type Stainless Steel ◽  
Rotary Instruments ◽  
Apical Extrusion ◽  
Working Length ◽  
Significant Difference

ABSTRACTObjectives: The purpose of this study was to compare in-vitro the amount of debris extruded apically from extracted teeth, using K3, Protaper rotary instruments and manual step-back technique.Methods: Forty five human single-rooted mandibular premolar teeth were randomly divided into 3 groups. The teeth in 3 groups were instrumented until reaching the working length with K3, Protaper rotary instruments and K-type stainless steel instruments with manual step-back technique, respectively. Debris extruded from the apical foramen was collected into centrifuge tubes and the amount was determined. The data obtained were analyzed using Kruskal-Wallis one-way analysis of variance and Mann-Whitney U tests, with P=.05 as the level for statistical significance.Results: Statistically significant difference was observed between K3, Protaper and step-back groups in terms of debris extrusion (P<.05). Step-back group had the highest mean debris weight, which was significantly different from the K3 and Protaper groups (P<.05). The lowest mean debris weight was related to K3 group, which was significantly different from the Protaper group (P<.05).Conclusions: Based on the results, all instrumentation techniques produced debris extrusion. The engine-driven Ni-Ti systems extruded significantly less apical debris than step-back technique. However, Protaper rotary instruments extruded significantly more debris than K3 rotary instruments. (Eur J Dent 2008;2:233-239)

scholarly journals Efficacy of Biodentine as an Apical Plug in Nonvital Permanent Teeth with Open Apices: An In Vitro Study

2015 ◽  
Vol 2015 ◽  
pp. 1-4 ◽  
Author(s):  
Mehmet Bani ◽  
Elif Sungurtekin-Ekçi ◽  
Mesut Enes Odabaş
Keyword(s):  
Root Canal ◽  
In Vitro Study ◽  
Permanent Teeth ◽  
Fluid Filtration ◽  
Anterior Teeth ◽  
Sealing Ability ◽  
Working Length ◽  
Significant Difference ◽  
Kolmogorov Smirnov

The aim of this study was to evaluate the apical microleakage of Biodentine and MTA orthograde apical plugs and to compare the effect of thickness of these biomaterials on their sealing ability. A total of eighty maxillary anterior teeth were used. The apices were removed by cutting with a diamond disc (Jota, Germany) 2 mm from the apical root end in an attempt to standardize the working length of all specimens to 15 ± 1 mm. Both materials were placed in 1–4 mm thickness as apical plugs root canal. Root canal leakage was evaluated by the fluid filtration technique. One-way ANOVA was used in order to determine normality of dispersal distribution of parameters; thereafter, results were analyzed by Kolmogorov-Smirnov test. Overall, between microleakage values of MTA and Biodentine regardless of apical plug thickness, no difference was observed. In terms of plug thickness, a statistically significant difference was observed between the subgroups of MTA and Biodentine (p<0.05). The apical sealing ability of Biodentine was comparable to MTA at any apical plug thickness.

scholarly journals Effectiveness of Three Rotary Niti Instruments and Hand Instrumentation in Removing Gutta Percha from Root Canals: an in vitro Study

2015 ◽  
Vol 03 (03) ◽  
pp. 152-158
Author(s):  
Varun Jindal ◽  
Ajay Chhabra ◽  
◽  
Keyword(s):  
In Vitro Study ◽  
Radiographic Analysis ◽  
Filling Material ◽  
Universal System ◽  
Root Canals ◽  
Gutta Percha ◽  
Significant Difference ◽  
Niti Instruments ◽  
Hand Instrumentation

Abstract Objectives: The study aimed to assess variation in the remaining filling material by radiographic analysis and the time taken for retreatment system, with H-files; the ProTaper Universal Retreatment System; Mtwo retreatment system and the SybronEndo K3. Methods: 120 human mandibular premolars with single straight root canals were prepared with rotary twisted files (up to size 40/0.04) using the crown down technique and filled by cold lateral condensation and the radiographs were taken. After one week, teeth were divided into four groups (n = 30). Gutta-percha was removed using H-files, the ProTaper Universal Retreatment System, Mtwo system and the K3 System. After retreatment radiographs were taken for each sample and the remaining material was calculated for each canal third. Results: Rotary NiTi instruments were significantly faster than the hand instrumentation technique in performing the retreatment. In radiographic analysis there was significant difference in between the groups for coronal third in ProTaper group and for apical third in H-file group. Conclusions: In the radiographic analysis the ProTaper Universal System left less filling material in the coronal third and the H-files left less filling material in apical third as compared to other groups. Rotary instruments worked faster than H files.

scholarly journals COMPARISON OF THE AMOUNT OF APICAL EXTRUSION OF BACTERIA FOLLOWING THE USE OF DIFFERENT INSTRUMENTATION TECHNIQUES – AN IN VITRO STUDY

2011 ◽  
Vol 01 (04) ◽  
pp. 27-32
Author(s):  
Mithra N. Hegde ◽  
Snehal Thatte
Keyword(s):  
Culture Media ◽  
In Vitro Study ◽  
Control Group ◽  
Group Iii ◽  
Root Canals ◽  
Apical Extrusion ◽  
Significant Difference ◽  
Group Ii ◽  
Group 1

Abstract Objectives: The objective of this study is to compare the amount of extrusion of bacteria beyond the apical foramen after instrumentation with Crown down and Step-back techniques using a manual and engine driven nickel-titanium instruments Materials and Methods: Seventy-five mandibular premolars with similar dimensions were used for the study. Access cavities prepared and root canals contaminated with a suspension of Enterococcus faecalis. The contaminated teeth were then divided into three experimental groups. Group 1(Crowndown group) divided into two: Group 1–A Hand files: root canals were instrumented using K-files and Group 1B – Rotary files: root canals were instrumented using ProTaper instruments. Group II (Step-back group) divided into two: Group II A– Hand files: root canals were instrumented using K-files and group II B–Rotary files: the root canals were instrumented using Light Speed LSX instruments. Group III (control group): no instrumentation was done.Bacteria were extruded after preparation were collected into vials, microbiological samples were incubated in culture media for 24hrs. The CFUs were determined for each sample. The data obtained was analyzed using the Kruskal-Wallis one way analysis of variance and Mann-Whitney U-tests. Result: There was a significant difference in the amount of bacteria extruded by both Crowndown and Step-back. The Step- back hand method extruded significantly more bacteria when compared with Crowndown hand technique. Conclusion: All instrumentation techniques extruded intracanal bacteria apically. There was a significant difference in both the engine driven instrumentation techniques, while the hand instrumentation by Step-back extruded more bacteria.

scholarly journals Performance of RaCe Instrumentation System in Curved Root Canals: A Comprehensive Analysis by Three Study Methods

2013 ◽  
Vol 24 (3) ◽  
pp. 230-234 ◽  
Author(s):  
Denise Piotto Leonardi ◽  
Gilson Blitzkow Sydney ◽  
Mario Tanomaru Filho ◽  
Flares Baratto-Filho ◽  
Samantha Schaffer Pugsley Baratto ◽  
...  
Keyword(s):  
Root Canal ◽  
Histological Analysis ◽  
Wilcoxon Test ◽  
Study Methods ◽  
Root Canals ◽  
Working Length ◽  
Apical Transportation ◽  
Significant Difference ◽  
Canal Enlargement ◽  
Curved Root Canals

In this study, curved maxillary molar root canals were instrumented with RaCe rotary system to evaluate: 1. the occurrence of canal transportation using a radiographic platform; 2. the action of the instruments on the dentin walls, centering ability and canal enlargement by analysis of digital images; and the percentage of regular dentin surfaces and debris within the canal by histological analysis. Ten mesiobuccal roots of extracted human maxillary molars were embedded in acrylic resin and sectioned at the middle and apical thirds. Root canal shaping was performed using the RaCe rotary system at 250 rpm and 1 Ncm torque. Each instrument set was used five times according to a crown-down technique in the following sequence: 40/0.10, 35/0.08, 25/0.06, 25/0.04, 25/0.02 (working length - WL), 30/0.02 (WL) and 35/0.02 (WL). Each instrument was inserted until resistance was felt and then pulled back, followed by brushing movements towards all canal walls. Each specimen was assessed by three study methods: radiographic platform, digitized image assessment and histological analysis. The radiographic platform showed lack of apical transportation. No statistically significant difference (Wilcoxon test, p>0.05) was found between the middle and apical thirds regarding instrument action on dentin walls, centering ability, area of root canal enlargement, percentage of regular dentin surfaces and debris within the root canal. It may be concluded that RaCe system is a suitable method for the preparation of curved root canals, regarding the maintenance of root canal original path, action on dentin walls, canal enlargement and removal of debris from the root canal lumen.

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