scholarly journals Effect of Cold Storage on Livability and Progressive Motility of Cauda Epididymal Spermatozoa of Red Sokoto Buck Recovered Post Mortem

2021 ◽  
Author(s):  
Faith Chinonye Eke ◽  
Terzungwe Ahemen
Keyword(s):  
Sperm Motility ◽  
Cold Storage ◽  
Standard Error ◽  
Storage Time ◽  
Post Mortem ◽  
Time Processing ◽  
Progressive Motility ◽  
Epididymal Spermatozoa

Post mortem spermatozoa recovery is an important tool for harvesting germplasm. The study aimed to determine the effect of cold storage time at 4°C for 48 and 72 hours on the quality of sperm of Red Sokoto buck caudal epididymaly spermatozoa in terms of motility and livability recovered postmortem. Fifteen testicles with attached epididymis were collected and processed at 0, 48, and 72 hours of storage. The samples were divided into 3 groups according to the time processing after collection ; 0 hour, 48 hours and 72 hours, with a total of 5 samples per group. Each storage group motility and livability was evaluated, mean and the standard error of their mean was recorded. Mean percent sperm motility declined significantly ( p< 0.05) as postmortem storage time increased ( motility: 79.00+/- 2.92%, 40.00+/- 4.18, 37.00+/- 3.39) .There was no significant (p> 0.05) decrease in livability from the 0 hour storage to the 48 hour and 72 hours storage ( 75.60+/- 4.40, 73.60+/- 3.84, 68.20+/- 2.51). The current study has shown that viable spermatozoa can be recovered after 48 hours of storage at 4˚C of the cauda epididymides Red Sokoto buck.

scholarly journals Sperm motility assessment of epididymal sperm from post mortem goat testicles held at 5°C

2017 ◽  
Vol 4 (2) ◽  
pp. 16
Author(s):  
Angelica C. Bumanlag ◽  
Hannah Lei M. Harada ◽  
Cynthia C. Divina ◽  
Marlon B. Ocampo ◽  
Lerma C. Ocampo
Keyword(s):  
Sperm Motility ◽  
Storage Time ◽  
Genetic Material ◽  
Holding Time ◽  
Considerable Proportion ◽  
Prolonged Storage ◽  
Post Mortem ◽  
Epididymal Sperm ◽  
Yield Quality ◽  
Slow Moving

Appropriate holding conditions for post mortem testicles of goat to yield quality epididymal sperm (ES) as a source of genetic material for cryobanking and fertilization studies are lacking. In this study, the effect of storage time on the motility of ES from post mortem testicles maintained at 5°C was evaluated. In the laboratory, the cauda epididymides were excised from the testicles after 4 hr (G-1) and 24 hr (G-2) of holding time before collecting the sperm in a Tris-citrate buffered solution and evaluated using a CASA. Sperm motility profiling revealed a subpopulation of static, slow, motile and progressive ES. The proportion of static sperm in the control (16.97±6.21) and G-1 (21.53±5.60) were lower significantly than G-2 (36.13±5.05). The proportion of slow moving sperm was lower significantly than G-1 (23.31±3.57) and G-2 (25.45±3.32). The proportion of motile and progressive motile sperm decreases significantly (P<0.05) as the holding time increases at 78.46±4.64% (G-1) to 63.85±4.06 (G-2) and 45.53±8.89 (G-1) to 25.46±8.42 (G-2), respectively. The results showed that prolonged storage of post mortem testicles at 5°C could result to a reduced percentage of motile and progressively motile ES. Nevertheless, this considerable proportion of ES remained useful both for cryobanking and fertilization studies.

scholarly journals Armazenamento úmido e seco de rosas cortadas

2016 ◽  
Vol 22 (2) ◽  
pp. 166
Author(s):  
Gláucia Moraes Dias ◽  
José Maria Monteiro Sigrist ◽  
Patricia Cia ◽  
Sylvio Luís Honório
Keyword(s):  
Cold Storage ◽  
Storage Time ◽  
Tap Water ◽  
Cut Flower ◽  
Dry Storage ◽  
Wet Storage ◽  
The World ◽  
Stem Quality

Rosa sp. is the leading cut flower commercialized in Brazil and in the world; and to maintain stem quality it should be stored at 1 °C, however, there are still doubts about the storage of roses, especially whether the stems should be cold stored in water before marketing. In order to assess the feasibility of wet and dry storage, rose stems, ‘Avalanche’ were stored for 28 days in both conditions at 1 ± 2 °C / 95% RH. Weekly stems batches were transferred to 25 °C / 70% RH and kept in vessels containing tap water for postharvest assessment. It was observed that the decrease in the quality of the roses was inversely proportional to the storage time in both treatments. Although wet storage can occur for up to 21 days, when you need to store roses for a week or two, cold storage in dry condition is advised since it is more economical and viable for the producer.

Effect of different extenders for preservation of ram semen at 4°C

2016 ◽  
Author(s):  
Haneef A. Rather ◽  
Rafiqul Islam ◽  
Asloob A. Malik ◽  
Farooz A. Lone ◽  
Mohamad Naiem Banday
Keyword(s):  
Citric Acid ◽  
Sperm Motility ◽  
Egg Yolk ◽  
Morphological Abnormalities ◽  
Progressive Motility ◽  
Hypoosmotic Swelling Test ◽  
Live Sperm

The aim of this study was to investigate the effects of different extenders viz. Tris citric acid fructose egg yolk (TCFEY), Tris citric acid glucose egg yolk (TCGEY), Egg yolk citrate fructose (EYCF) and Egg yolk citrate glucose (EYCG) on the quality of ram spermatozoa during preservation at 4°C. Semen samples showing more than 3+ mass motility and 70% progressive motility were pooled and subsequently divided into four aliquots. Each aliquot was extended separately in four different extenders viz. TCFEY, TCGEY, EYCF and EYCG and stored at 4°C up to 72h. The quality of spermatozoa on the basis of percentage of sperm motility, live sperm, morphological abnormalities, intact acrosome and hypoosmotic swelling test (HOST) reacted spermatozoa was evaluated immediately after extension in particular extenders (0 h), 24 h, 48 h and 72 h after preservation at 4°C. The percent sperm motility was significantly (P<0.01) higher for TCFEY and TCGEY than EYCF and EYCG at 72 h of preservation at 4°C. The percent HOST reacted spermatozoa and intact acrosomes were significantly (P<0.01) higher and morphological abnormalities were significantly (P<0.01) lower for Tris based fructose extender than other three extenders at 72 h at 4°C. In conclusion, Tris citric acid fructose egg yolk (TCFEY) was found the best in maintaining the quality of ejaculated ram spermatozoa during preservation for 72 h at 4°C. 

scholarly journals Influence of pentoxifylline and caffeine on stallion epididymal sperm motility after thawing

2021 ◽  
Vol 90 (3) ◽  
pp. 271-276
Author(s):  
Miroslava Mráčková ◽  
Lenka Vojtěchovská ◽  
Petra Andrlová ◽  
Šárka Krisová ◽  
Markéta Sedlinská
Keyword(s):  
Sperm Motility ◽  
The Other ◽  
Epididymal Sperm ◽  
Progressive Motility ◽  
Epididymal Spermatozoa ◽  
Positive Effect

Two substances at two concentrations each were added to frozen-thawed stallion epididymal spermatozoa to improve motility indicators, which were assessed 30, 60, 120, 180 and 240 min after thawing. Pentoxifylline was added at concentrations of 3.6 mM (1 mg/ml) and 7.18 mM (2 mg/ml). Both concentrations had a positive effect on total and progressive motility of spermatozoa throughout the study. The other substance, caffeine, was added at concentrations of 2 mM (0.4 mg/ml) and 5.5 mM (1 mg/ml). Both concentrations had a positive effect on total and progressive motility of spermatozoa only 30 and 60 min after thawing. Subsequently, 180 min after thawing, improvement was found only in total sperm motility.

scholarly journals Reactive oxygen species and evaluation of the quality of carcasses and beef meat from cold storage slaughterhouses located in the Federal District and Surroundings (Brazil)

2018 ◽  
Author(s):  
Thais Rezende Leite ◽  
Carla Magalini Zago de Sousa ◽  
Paulo Eduardo Narciso de Souza ◽  
Fabiane Hiratsuka Veiga de Souza ◽  
Vítor Salvador Picão Gonçalves ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cold Storage ◽  
Ph Value ◽  
Reactive Oxygen ◽  
Federal District ◽  
Influential Factors ◽  
Oxygen Species ◽  
Post Mortem ◽  
Beef Meat

The aim of this study was to detect reactive oxygen species and evaluate the quality of carcasses and beef meat from cold storage slaughterhouses located in the Federal District and Surroundings. Information was obtained on the gender, breed and age of each animal, as well as the distance travelled (km) and time (h) from the property to the cold storage slaughterhouse. Data and samples were obtained from a total of 33 animals and their respective carcasses. Fragments of the extensor carpi radialis muscle were extracted from all the carcasses to analyze the presence of reactive oxygen species (ROS), and samples of the Longissimus dorsi muscle to perform the 24h post-mortem pH, colorimetric test, cooking loss and drip loss assessment and to measure the shear force. The presence of hematomas was detected in 28 carcasses, where the tail and croup (17/33) and flank (17/33) regions were the most affected. The electron paramagnetic resonance indicated an average of 52.59 ROS/g in the analyzed pieces. The meat quality tests indicated averages of: 5.8 for the 24h post-mortem pH, L*29.34, a* 2.52 and b* 1.31 in calorimetry, 2.30 kg/f for the shearing force, 11.75% of cooking losses and 1.88% of drip losses. The statistical analyses demonstrated a tendency to positive correlation between the presence of hematomas with and the amount of ROS, and between the presence of hematomas and pH value. Furthermore, statistically, female gender was one of the influential factors on the tenderness value. According to the results, it was concluded that the meat evaluated in this study meets the desirable quality parameters and it was possible to detect reactive oxygen species in the samples of muscle tissue.

scholarly journals Changes in Physicochemical Properties of Wild and Cultivated Blackberry during Postharvest Cold Storage

2021 ◽  
Author(s):  
N. Azzouzi ◽  
S. Zantar ◽  
N. Aghmir ◽  
M.R. Britel ◽  
A. Maurady
Keyword(s):  
Physicochemical Properties ◽  
Cold Storage ◽  
Skin Color ◽  
Physicochemical Parameters ◽  
Storage Time ◽  
Titratable Acidity ◽  
Plant Materials ◽  
The North ◽  
Rubus Fruticosus

Background: Blackberry is a seasonal harvested fruit that is also very fragile and perish- es quickly. A comparative study was conducted to find out influence of cold storage on physicochemical parameters of wild and cultivated blackberry over a period of 12 days. Methods: The plant materials were composed of the blackberry fruit, including a compound of cultivated blackberry (Rubus spp.), and wild blackberry (Rubus fruticosus L. agg) which were harvested in the North of Morocco. The temperature of the storage of wild and cultivated blackberry was 5 °C for 4, 8, and 12 days, and then transferred to 25 °C for 1 day to simulate transport and commercialization. After that, the physicochemical parameters were analyzed. Statistical analyses were performed using SAS. Results: During 12 days of storage, the pH of samples was decreased from 3.69 to 3.22 for wild blackberry, and from 4.85 to 3.43 for cultivated blackberry. The titratable acidity was increased from 1.61 to 3.28 for wild blackberry, and from 2.07 to 3.25 for cultivated blackberry. Flavonoids also showed a remarkable increase in values from 30 to 70.66 mg QE/100g of wild blackberries and from 25.33 to 60.66 mg QE/100g in cultivated ones between harvest and the last day of storage. The variation in skin color revealed a decrease during storage of L* brightness, a* redness, and yellowness b* for both blackberries. Conclusion: The most suitable storage time of blackberry components during cold storage at 5 °C was 12 days. The temperature at 5 °C preserves the quality of the blackberry for both wild and cultivated ones. However, the wild blackberry was more resistant than cultivated one to cold storage.

Effect of storage time on the quality of cauda epididymal spermatozoa of West African dwarf (WAD) rams

2019 ◽  
Vol 205 ◽  
pp. 144-149
Author(s):  
Ndumari Wachida ◽  
Umoren Emekopobong Bassey ◽  
Philip Makama Dawuda
Keyword(s):  
Storage Time ◽  
West African ◽  
West African Dwarf ◽  
Epididymal Spermatozoa

Motility, progressive motility score and DNA integrity of spermatozoa from cold-preserved mouse cauda epididymis

Zygote ◽  
2017 ◽  
Vol 25 (2) ◽  
pp. 160-167
Author(s):  
Man-Xi Jiang ◽  
Yan Zhu ◽  
Xiang-Hong Ou ◽  
Lei-Ning Chen ◽  
Tao Jing ◽  
...  
Keyword(s):  
Comet Assay ◽  
Cold Storage ◽  
Storage Time ◽  
Dna Integrity ◽  
Dna Denaturation ◽  
Short Term ◽  
Suitable Alternative ◽  
Fertilization Rates ◽  
Progressive Motility ◽  
Reproductive Ability

SummaryThis study attempted to investigate and validate whether epididymis cold storage could be a suitable alternative for short-term preservation of spermatozoa. Mouse cauda epididymides and spermatozoa were preserved at 4–8°C from 1 day to 6 weeks. From days 1 to 10, motility and fertility were daily examined when motility loss occurred. From week 1, spermatozoa were used for intracytoplasmic sperm injection (ICSI) at weekly intervals to test their fertility, and spermatozoa DNA integrity was determined by comet assay. We found that motility and progressive motility scores gradually decreased with storage time. In nearly all spermatozoa, DNA integrity was maintained from days 1 to 10, but the percentage of spermatozoa with damaged DNA significantly increased from week 2 to week 6. Spermatozoa retained fertility until day 6, although fertility gradually decreased after day 3. From week 1 to week 5, fertilization rates by ICSI were more than 82.69% but decreased gradually after week 3. We found that spermatozoa preserved in the epididymis at 4–8°C had progressively lower motility, fertility and proportion of undamaged DNA, but could still fertilize oocytes. However, all the parameters of cold-preserved spermatozoa were completely inferior to that from cold-preserved cauda epididymides. The results imply that cold storage of cauda epididymides could be conducive to short-term preservation of spermatozoa, and the cold-stored spermatozoa can resist DNA denaturation, which is necessary for maintaining reproductive ability.

scholarly journals Viability of ovine spermatozoa collected from epididymides stored at 18°-25°C for 48 hours post mortem

2018 ◽  
Vol 70 (4) ◽  
pp. 1023-1028
Author(s):  
T.G. Bergstein-Galan ◽  
R.R. Weiss ◽  
T.S.R. Barbosa ◽  
L.E. Kozicki ◽  
S.D. Bicudo
Keyword(s):  
Morphological Changes ◽  
Membrane Integrity ◽  
Post Mortem ◽  
Integrity Test ◽  
Vitro Fertilization ◽  
Progressive Motility ◽  
Viable Cells ◽  
Epididymal Spermatozoa ◽  
Artificial Vagina

ABSTRACT The objectives of this study were to verify the time during which viable ovine spermatozoa could be recovered from the cauda epididymis kept at ambient temperature (18-25°C). Sperm collected in an artificial vagina (AV) were used as control. Spermatozoa samples were collected with an AV and from epididymis at 0 (G0), 6 (G6), 12 (G12), 24 (G24), and 48 (G48) hours post mortem. Total motility (TM), progressive motility (PM), hypo-osmotic membrane integrity test (HOST) and morphological changes were assessed. TM decreased (P<0.05) from 24 hours post mortem (70.0±1.9%) compared to AV (86.4±1.0%). PM decreased (P<0.05) from 12 hours after death (31.3±4.0%) compared to AV group (73.2±1.4%). The percentage of viable cells in HOST decreased (P<0.05) in the G48 (60.0±8.9%). Spermatozoa recovery was lower (P<0.05) 48 hours after death (2064.2±230.7 x 106 spermatozoa) compared to G0(2623.6±288.4 x 106 spermatozoa). In conclusion, under the conditions of this study, it would be possible to use epididymal spermatozoa recovered up to 24 hours after death for artificial insemination or in vitro fertilization; however, fertility trials are necessary to prove this hypothesis.

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