scholarly journals Pleomorphic Adenoma versus Basal Cell Adenoma: An immunohistochemical analysis with b-catenin

2014 ◽  
Vol 17 (1) ◽  
pp. 23
Author(s):  
Renata Falchete do Prado ◽  
Camila Lopes Cardoso ◽  
Alberto Consolaro ◽  
Antonio Luis Assis Taveira
Keyword(s):  
Basal Cell ◽  
Immunohistochemical Analysis ◽  
Myoepithelial Cells ◽  
Complex Method ◽  
Nuclear Staining ◽  
Cell Adenoma ◽  
Formalin Fixed Paraffin ◽  
Pleomorphic Adenomas ◽  
Formalin Fixed Paraffin Embedded ◽  
Degree Of Differentiation

<p>The objective of this study was to investigate the distribution of b-catenin in pleomorphic adenomas and the basal cell adenomas to<strong> </strong>clarify its possible role in the etiopathogenesis of these two lesions. The expression of b-catenin (BD Transduction Laboratories) was analyzed by immunohistochemistry in formalin-fixed, paraffin-embedded specimens by the avidin-biotin-peroxidase complex method in 10 pleomorphic adenomas and 2 basal cell adenomas. The specimens were analyzed taking into account intensity, distribution and association with myoepithelial cells. The results showed that  all cases of pleomorphic adenomas exhibited membranous and cytoplasmic immunostaining and the 2 cases of basal cell adenomas displayed nuclear staining. Higher b-catenin index rates were seen mainly in ductal structures of pleomorphic adenomas and in the nuclei of myoepithelial stromal and myoepithelial cells of solid clusters in basal cell adenomas. In conclusion, this immunohistochemical study may suggests the different degree of differentiation of the myoepithelial cells in these two tumors.<strong><em></em></strong></p>

Differential diagnosis of pleomorphic adenoma by immunohistochemical means

1991 ◽  
Vol 105 (12) ◽  
pp. 1057-1060 ◽  
Author(s):  
Toshiro Nishimura ◽  
Mitsuru Furukawa ◽  
Ei Kawahara ◽  
Atsuo Miwa
Keyword(s):  
Pleomorphic Adenoma ◽  
Basal Cell ◽  
Diagnostic Value ◽  
Secretory Component ◽  
Major Salivary Gland ◽  
Diagnostic Dilemma ◽  
Cell Adenoma ◽  
Salivary Gland Tumours ◽  
Adenoid Cystic ◽  
Pleomorphic Adenomas

AbstractImmunohistochemical study of major salivary gland tumours was performed on 60 pleomorphic adenomas, five basal cell adenomas and 10 adenoid cystic carcinomas to determine the diagnostic value of each antigen. Immunoreactivity examined were intermediate filaments (keratin, vimentin, desmin and glial fibrillary acidic protein [GFAP]) and related substances (actin, S-100 protein and secretory component). In pleomorphic adenomas, there was positive immunoreactivity for GFAP which was not observed in normal tissue or other neoplastic tissues. Immunoreactivity of GFAP was closely related to myxomatous and early chondromatous differentiation in pleomorphic adenoma. It is considered that GFAP immunoreactivity should be assessed in the occasional differential diagnostic dilemma of pleomorphic adenoma versus adenoid cystic carcinoma and basal cell adenoma, because of its ability to show potential and definite myxochondromatous differentiation.

scholarly journals Reliable LC3 and p62 autophagy marker detection in formalin fixed paraffin embedded human tissue by immunohistochemistry

2015 ◽  
Vol 59 (2) ◽  
Author(s):  
A.M. Schläfli ◽  
S. Berezowska ◽  
O. Adams ◽  
R. Langer ◽  
M.P. Tschan
Keyword(s):  
Human Tissue ◽  
Nuclear Staining ◽  
Double Positive ◽  
Tissue Samples ◽  
Development Of Resistance ◽  
Autophagy Induction ◽  
Formalin Fixed Paraffin ◽  
Unknown Significance ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Autophagy assures cellular homeostasis, and gains increasing importance in cancer, where it impacts on carcinogenesis, propagation of the malignant phenotype and development of resistance. To date, its tissue-based analysis by immunohistochemistry remains poorly standardized. Here we show the feasibility of specifically and reliably assessing the autophagy markers LC3B and p62 (SQSTM1) in formalin fixed and paraffin embedded human tissue by immunohistochemistry. Preceding functional experiments consisted of depleting LC3B and p62 in H1299 lung cancer cells with subsequent induction of autophagy. Western blot and immunofluorescence validated antibody specificity, knockdown efficiency and autophagy induction prior to fixation in formalin and embedding in paraffin. LC3B and p62 antibodies were validated on formalin fixed and paraffin embedded cell pellets of treated and control cells and finally applied on a tissue microarray with 80 human malignant and non-neoplastic lung and stomach formalin fixed and paraffin embedded tissue samples. Dot-like staining of various degrees was observed in cell pellets and 18/40 (LC3B) and 22/40 (p62) tumors, respectively. Seventeen tumors were double positive for LC3B and p62. P62 displayed additional significant cytoplasmic and nuclear staining of unknown significance. Interobserver-agreement for grading of staining intensities and patterns was substantial to excellent (kappa values 0.60 - 0.83). In summary, we present a specific and reliable IHC staining of LC3B and p62 on formalin fixed and paraffin embedded human tissue. Our presented protocol is designed to aid reliable investigation of dysregulated autophagy in solid tumors and may be used on large tissue collectives.

scholarly journals Basal Cell Adenoma: About Four Cases

2020 ◽  
Vol 3 (1) ◽  
Keyword(s):  
Basal Cell ◽  
The Elderly ◽  
Myoepithelial Cells ◽  
Medium Size ◽  
Basal Cell Adenoma ◽  
Cell Adenoma ◽  
Basal Cell Adenocarcinoma ◽  
Palisade Cell ◽  
Cell Base

Benign tumor of the salivary glands, composed of basaloid cells of architecture most often ductal and tubular or in nests (OMS 2017). We report the case of 4 patients carrying basal cell adenoma diagnosed at the department of pathological anatomy CHU MOHAMMED VI MARRAKECH. These are three women and one man. The average age was 49 years old. The clinical examination found a unilateral parotid mass gradually increasing in size. All the patients benefited from an ultrasound which was in favor of a homogeneous hypoechoic lobulated formation with posterior reinforcement evoking a pleomorphic adenoma. The patients benefited from a total parotidectomy. Macroscopic examination found a thick-walled, hemorrhagic-cystic formation in one patient. Microscopic examination showed a benign encapsulated tumor proliferation of compact architecture, in situ clusters and in channels with eosinophilic contents. . The epithelial cells have a basaloid appearance bordered at the periphery by a palisade cell base. They are of small to medium size, provided with ovoid cores with fine chromatin. The cytoplasm is abundant basophilic. The myoepithelial cells are sometimes cuboid, sometimes fusiform. The nuclei are discreetly elongated hyperchromic. The cytoplasm is scarce eosinophilic.The basal cell adenoma is a rare salivary tumor representing less than 3.7%. It occurs mainly in the elderly with a range of 57 to 70 years. Clinically, it is in most cases a mobile solitary mass of firm consistency. The tumor has a monomorphic appearance due to the predominance of basaloid cells. The differential diagnosis is primarily with basal cell adenocarcinoma and adenoid cystic carcinoma.

PD-L1 Expression on Cell Block Preparations of Pancreatic Adenocarcinoma

2019 ◽  
Vol 152 (Supplement_1) ◽  
pp. S94-S95
Author(s):  
Victoria Costa ◽  
David Kim ◽  
Melanie Johncilla ◽  
Abha Goyal ◽  
Rema Rao
Keyword(s):  
Disease Progression ◽  
Tumor Cells ◽  
Locally Advanced ◽  
Immunohistochemical Analysis ◽  
Inflammatory Cells ◽  
Fixation Method ◽  
Lung Cancers ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Combined Positive Score

Abstract Objectives Programmed death-ligand 1 (PD-L1) is an emerging molecular target in anticancer therapy, most notably non–small cell lung cancers. PD-L1 expression in pancreatic adenocarcinomas (PDAs) on surgical specimens is highly variable, with 10% to 60% of tumors showing expression. PD-L1 expression in PDA on endoscopic ultrasound-guided fine needle aspirations (EUS-FNAs) has been rarely studied. Methods Formalin-fixed, paraffin embedded (FFPE) cell blocks (CBs) from 65 EUS-FNA samples of 55 patients, with a diagnosis of PDA, with at least 20% tumor cellularity were retrieved. The cell blocks were originally fixed in CytoRich fixative. Immunohistochemical staining (IHC) for PD-L1 was performed using the M365329-1 (22C3) clone according to the manufacturer’s approved protocol and optimized for the fixation method using appropriate controls. A combined positive score (CPS) defined per CAP guidelines as the total number of positive tumor cells and inflammatory cells as a percentage of the total number of tumor cells was assessed for each case and was grouped as <1, 1-20, or >20. Results Twenty-five samples (38%) from 21 patients showed immunoreactivity to PD-L1, with 21 (32%) having a CPS of <1 and four (6%) having a CPS of 1-20. Eight of these 25 samples had surgical correlates, of which concordant staining was noted in five (62.5%). Of the discordant three, decreased tumor cell sampling in the core biopsy was noted in one. Overall, 20 of 21 patients (95%) with PD-L1 immunoreactivity had disease progression with 17 (81%) associated with metastatic disease and three (14%) with locally advanced disease. Conclusion Immunohistochemical analysis for PD-L1 is feasible on EUS-FNA CB samples when optimized and validated for the fixation method using appropriate controls. PD-L1 expression is seen in only a minority of PDAs, albeit with a very low CPS. The potential role of PD-L1 as a prognostic marker for disease progression needs further exploration.

scholarly journals Detection of Mycoplasma ovipneumoniae and Pasteurella haemolytica Antigens by an Immunoperoxidase Technique in Pneumonic Ovine Lungs

1996 ◽  
Vol 33 (1) ◽  
pp. 74-76 ◽  
Author(s):  
R. Haziroglu ◽  
K. S. Diker ◽  
J. Turkarslan ◽  
M. Y. Gulbahar
Keyword(s):  
Epithelial Cells ◽  
Pasteurella Haemolytica ◽  
Histologic Examination ◽  
Complex Method ◽  
Immunoperoxidase Technique ◽  
Tissue Sections ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed ◽  
Mycoplasma Ovipneumoniae

Four hundred twenty pneumonic lungs from lambs were examined for Mycoplasma ovipneumoniae and Pasteurella haemolytica by an immunoperoxidase technique using an extravidin-biotin-peroxidase complex method in formalin-fixed, paraffin-embedded sections. Histologic examination of tissue sections revealed strong positive reactions in 60.9% and 68.3% of the lungs against M. ovipneumoniae and P. haemolytica, respectively. M. ovipneumoniae and P. haemolytica antigens were observed at the surface and/or within the epithelial cells, macrophages, leucocytes, and bronchiolar exudate. The location of M. ovipneumoniae in the cytoplasm of the epithelial cells and P. haemolytica in the neutrophils was detected immunohistochemically.

The role of miRNA-21 and epithelial mesenchymal transition (EMT) process in colorectal cancer

2016 ◽  
Vol 70 (4) ◽  
pp. 331-356 ◽  
Author(s):  
Anelisa Jaca ◽  
Padmini Govender ◽  
Michael Locketz ◽  
Richard Naidoo
Keyword(s):  
Colorectal Cancer ◽  
Epithelial Mesenchymal Transition ◽  
Immunohistochemical Analysis ◽  
Clinicopathological Features ◽  
Mesenchymal Transition ◽  
Expression Levels ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Sporadic Cases ◽  
E Cadherin

AimsThe study was conducted to assess the expression levels of epithelial mesenchymal transition (EMT) proteins (E-cadherin, N-cadherin, snail-1 and vimentin) and miRNA-21. In addition, we correlated these data with clinicopathological features in Colorectal cancer.MethodsH&E slides from a total of 59 formalin fixed paraffin embedded tissue blocks were examined by a pathologist to demarcate normal and tumour regions. Immunohistochemical analysis of mismatch repair proteins (MLH1, MSH2 and MSH6) and EMT markers (E-cadherin, N-cadherin, snail-1 and vimentin) was performed. The miRNA-21 expression levels were determined using qRT-PCR and the data was analysed using the relative quantification method. The Fisher's exact and Pearson's χ2 tests were used to correlate snail-1, E-cadherin, miRNA-21 and clinicopathological data.ResultsOur results showed a statistically significant correlation between high miRNA-21 expression levels and E-cadherin positive cases. There was also an association between high miRNA-21 expression levels and negative snail-1 expression. No significant correlation was seen between miRNA-21 expression levels and clinicopathological features. Moreover, high expression levels of miRNA-21 were significantly associated with the sporadic cases.ConclusionsOur data suggest that miRNA-21 in association with E-cadherin and snail-1 does not play a significant role in the development and progression of this disease.

The cellular composition of basal cell adenoma of the parotid gland: An immunohistochemical analysis

1990 ◽  
Vol 70 (5) ◽  
pp. 619-626 ◽  
Author(s):  
Ikuko Ogawa ◽  
Hiromasa Nikai ◽  
Takashi Takata ◽  
Mutsumi Miyauchi ◽  
Hiroshi Ito ◽  
...  
Keyword(s):  
Parotid Gland ◽  
Basal Cell ◽  
Immunohistochemical Analysis ◽  
Cellular Composition ◽  
Basal Cell Adenoma ◽  
Cell Adenoma

scholarly journals Immunoreactivity of A103, An Antibody to Melan A, in Canine Steroid-Producing Tissues and their Tumors

2001 ◽  
Vol 13 (4) ◽  
pp. 328-332 ◽  
Author(s):  
José A. Ramos-Vara ◽  
Marilyn E. Beissenherz ◽  
Margaret A. Miller ◽  
Gayle C. Johnson ◽  
John M. Kreeger ◽  
...  
Keyword(s):  
Leydig Cells ◽  
Nuclear Staining ◽  
Normal Tissues ◽  
Adrenocortical Adenomas ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Adrenocortical Carcinomas ◽  
Formalin Fixed ◽  
Melanocytic Differentiation ◽  
Sertoli Cell Tumors

The monoclonal antibody A103 to the melanocytic differentiation antigen Melan A stains human steroid-producing cells and their tumors. A total of 200 formalin-fixed, paraffin-embedded canine normal tissues and hyperplastic and neoplastic lesions of the adrenal gland, testis, and ovary were immunohistochemically tested for Melan A with antibody A103. Leydig cell tumors (23/23, 100%), Sertoli cell tumors (14/15, 93%), and adrenocortical adenomas (12/13, 92%) were consistently positive. Adrenocortical carcinomas (23/35, 65%) and granulosa cell tumors (10/17, 59%) were less frequently positive. All pheochromocytomas, seminomas, and dysgerminomas were negative. The pattern of staining was cytoplasmic, but nuclear staining was also frequently seen in normal Leydig cells and their tumors. As in human tumors, immunohistochemistry for Melan A stains many canine steroid-producing tumors and can be used to distinguish these tumors from those of nonstereidogenic cells.

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