Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest
<p><strong>Objective: </strong>This study evaluated the effect of rosemary extract on <em>Candida albicans</em>, <em>Staphylococcus aureus</em>, <em>Enterococcus faecalis</em>, <em>Streptococcus mutans</em> and <em>Pseudomonas aeruginosa </em>monomicrobial biofilms viability, as well as on <em>C. albicans </em>associated with <em>S. aureus</em>, <em>E. faecalis</em>, <em>S. mutans</em> or <em>P. aeruginosa </em>in polymicrobial biofilms. <strong>Material and Methods: </strong>In microtiter plate, mono- and polymicrobial biofilms for 48 h were formed. Then, they were exposed for 5 min to rosemary extract (200 mg/mL). Saline (0.9% NaCl) was used as control. After, washes were done with saline to remove the affected cells. Biofilm viability was checked by MTT colorimetric assay, after treatment. Absorbance of the wells was read in microplate spectrophotometer (570 nm) and data were converted to reduction percentage and statistically analyzed by ANOVA and Tukey test (<em>p</em> ≤ 0.05). <strong>Results:</strong> After application of rosemary extract, with exception of the <em>E. faecalis</em> biofilm, significant reductions in mono- and polymicrobial biofilms viability were observed. <strong>Conclusion: </strong><em>C. albicans</em>, <em>S. aureus</em>, <em>S. mutans</em> and <em>P. aeruginosa</em> monomicrobial biofilms were affected by rosemary extract, as well as <em>C. albicans</em> associated with <em>S. aureus</em>, <em>E. faecalis</em>, <em>S. mutans</em> or <em>P. aeruginosa</em> in polymicrobial biofilms, presenting significant viability reductions. </p>