scholarly journals Propagação in vitro de cultivares de alpinia em diferentes fontes de luz

2019 ◽  
Vol 25 (1) ◽  
pp. 49-54
Author(s):  
Marcos Vinícius Marques Pinheiro ◽  
Denise Schmidt ◽  
Maria Inês Diel ◽  
Jullie Dos Santos ◽  
Leonardo Antonio Thiesen ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Plant Production ◽  
Experimental Unit ◽  
Light Sources ◽  
White Led ◽  
Light Emitting ◽  
Randomized Design ◽  
Red Ginger ◽  
Alpinia Purpurata

The light source in tissue culture is often one of the punctual factors for the adequacy of plant production. For this, light emitting diodes (LEDs) have been used in order to increase the growth and development of the plants propagated in vitro. The objective was to evaluate the in vitro propagation of cultivars of Alpinia purpurata ‘Red Ginger’ and ‘Pink Ginger’ in two different light sources (fluorescent or LEDs tubes). The experiment was conducted in a completely randomized design, factorial scheme (2x2), totaling four treatments with ten replicates, and the experimental unit composed of one flask containing five explants. After 55 days, white LED and the ‘Red Ginger’ provided the highest number of shoots. For the number of leaves, the cultivar ‘Red Ginger’ and white LED lamps provided superior results. For shoot length (cm), the cultivar ‘Red Ginger’ kept under fluorescent lamps was superior, and ‘Pink Ginger’ presented superiority when cultivated under LED lights. Thus, even with differentiated responses among genotypes, the use of white LED lamps in the in vitro propagation of Alpinia purpurata ‘Red Ginger’ and ‘Pink Ginger’ cultivars is suggested.

scholarly journals Application of Light-Emitting Diodes for Improving the Nutritional Quality and Bioactive Compound Levels of Some Crops and Medicinal Plants

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1477
Author(s):  
Woo-Suk Jung ◽  
Ill-Min Chung ◽  
Myeong Ha Hwang ◽  
Seung-Hyun Kim ◽  
Chang Yeon Yu ◽  
...  
Keyword(s):  
Plant Growth ◽  
In Vitro Culture ◽  
Light Emitting Diodes ◽  
Photosynthetic Pigment ◽  
Antioxidant Properties ◽  
Light Sources ◽  
Light Emitting ◽  
Cell Defense ◽  
Led Irradiation

Light is a key factor that affects phytochemical synthesis and accumulation in plants. Due to limitations of the environment or cultivated land, there is an urgent need to develop indoor cultivation systems to obtain higher yields with increased phytochemical concentrations using convenient light sources. Light-emitting diodes (LEDs) have several advantages, including consumption of lesser power, longer half-life, higher efficacy, and wider variation in the spectral wavelength than traditional light sources; therefore, these devices are preferred for in vitro culture and indoor plant growth. Moreover, LED irradiation of seedlings enhances plant biomass, nutrient and secondary metabolite levels, and antioxidant properties. Specifically, red and blue LED irradiation exerts strong effects on photosynthesis, stomatal functioning, phototropism, photomorphogenesis, and photosynthetic pigment levels. Additionally, ex vitro plantlet development and acclimatization can be enhanced by regulating the spectral properties of LEDs. Applying an appropriate LED spectral wavelength significantly increases antioxidant enzyme activity in plants, thereby enhancing the cell defense system and providing protection from oxidative damage. Since different plant species respond differently to lighting in the cultivation environment, it is necessary to evaluate specific wavebands before large-scale LED application for controlled in vitro plant growth. This review focuses on the most recent advances and applications of LEDs for in vitro culture organogenesis. The mechanisms underlying the production of different phytochemicals, including phenolics, flavonoids, carotenoids, anthocyanins, and antioxidant enzymes, have also been discussed.

scholarly journals Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish

2016 ◽  
Vol 14 (1) ◽  
Author(s):  
Eduardo Antônio Sanches ◽  
Danilo Caneppele ◽  
Renan Yoshiharu Okawara ◽  
Danielle Zanerato Damasceno ◽  
Robie Allan Bombardelli ◽  
...  
Keyword(s):  
Significant Interaction ◽  
Water Volume ◽  
Experimental Unit ◽  
Vitro Fertilization ◽  
Endangered Fish ◽  
Fertilization Rates ◽  
Randomized Design ◽  
Artificial Fertilization ◽  
Hatching Rates

Abstract The Steindachneridion parahybae is an endangered catfish from Brazil and strategies applied for gametes optimization are necessary. The aim of this study was to assess inseminating doses and water volume upon the fertilization, hatching rates and percentage of normal larvae in S. parahybae . Was used a randomized design in factorial scheme (4×4) with four inseminating doses: 1.0×104, 1.0×105, 1.0×106, 1.0×107spermatozoa oocyte-1 and four volumes of water: 1, 35, 65 and 95mL of water g-1 of oocytes. The combination of doses and volumes were performed in triplicates (n=48). Each incubator (1.5L of useful volume) with 1g of oocytes was considered as an experimental unit. Significant interaction between inseminating doses and volumes of water to the values of the fertilization rates and quadratic effect of doses and volume for the values of hatching rates were observed. The doses and volumes did not influence the percentage of normal larvae (87.70±5.06%). It is recommended the use of 5.5×106 spermatozoa oocyte-1 and 1mL of water g-1 of oocytes during in vitro fertilization procedure. These results allowed us to develop new biotechnological strategies applied to the conservation of S. parahybae .

scholarly journals IN VITRO DEVELOPMENT OF YELLOW LAPACHO (BIGNONIACEAE) USING HIGH-POWER LIGHT EMITTING DIODE

2018 ◽  
Vol 42 (5) ◽  
Author(s):  
Ezequiel Enrique Larraburu ◽  
Gonzalo Sanchez Correa ◽  
Berta Elizabet Llorente
Keyword(s):  
In Vitro Culture ◽  
High Power ◽  
Stomatal Density ◽  
Light Emitting Diode ◽  
Dry Weight ◽  
Light Sources ◽  
Multiplication Rate ◽  
Light Emitting ◽  
Shoot Dry Weight

ABSTRACT Handroanthus ochraceus (yellow lapacho) is a medicinal, ornamental and timber tree which can be propagated by in vitro culture. Conventional methods use fluorescent lighting (FL), whereas light emitting diode (LED) has been used for this purpose only recently. The aim of this work was to evaluate the effects of FL and high-power LED (HP-LED) on the in vitro multiplication and rooting of yellow lapacho at different irradiances (15 to 60 µmol m-2s-1). Epicotyls obtained from half-siblings was multiplicated in WPM (Woody Plant Medium) supplemented with 20 µM benzilaminopurine and 1 mM IBA (indolebutiric acid). For rooting, shoots were cultured for 3 days in ½WPM supplemented with 50 µM IBA and for 42 days in auxin-free ½WPM under HP-LED or FL lighting. Under HP-LED, the multiplication rate of shoots increased significantly (61%) from 20 to 40 µmol m-2s-1 respect to FL. Differences in abaxial stomatal density and size were observed between light sources at 20 µmol m-2s-1. High HP-LED irradiance produced the highest rooting percentage. In the rooting stage, the marginal means of treatments without factors interaction showed that HP-LED irradiances significantly increased shoot length by 20%, shoot fresh weight by 77% and shoot dry weight by 30% in comparison to the values under FL. The maximum values calculated from the regression curves were around 50 µmol m-2 s-1 for HP-LED for all parameters except root lenght whereas were around 20 µmol m-2 s-1 for FL for all parameters except fresh and dry weigth of shoot. Here we show that HP-LED lighting improve in vitro culture of H. ochraceus, reduced 81% energy consumption respect to FL and uses only a multispectral LED instead of different single color LEDs. Therefore, HP-LED could be useful for the micropropagation of tree species contributing to sustainable agriculture and ecological restoration of degraded areas.

Brassinolide on In Vitro Growth of Apical Meristem of Bananas

2014 ◽  
Vol 8 (1) ◽  
Author(s):  
FLORENDA C. BALLESTEROS-TEMANEL
Keyword(s):  
Plant Growth ◽  
In Vitro Propagation ◽  
Apical Meristem ◽  
Basal Medium ◽  
Tissue Growth ◽  
Shoot Length ◽  
New Class ◽  
Randomized Design ◽  
Plant Growth Substances

Brassinosteroids (BRs) are new class of hormones noted to perform multiplephysiological functions in plant growth and development and have the potentialof influencing cell and tissue growth in vitro. Many naturally occurring BRs,including brassinolide, have been discovered, their mode of action and their growthpromoting activities on plants. The use of brassinolide in in vitro propagation isnew. The Murashige and Skoog (1962) medium was used as basal medium. Plantgrowth regulators - IAA, BA and BR - were added to the medium. The study usedthe Completely Randomized Design (CRD) in factorial with three replications.The cultivar of banana and plant growth substances affected the number of budsproduced, shoot length, root length, and stem girth. The interaction of thesetwo factors (cultivar x PGR) influenced the number of buds produced in vitroand the shoot length of the meriplants. The study shows that brassinolide has aninfluence on shoot induction, proliferation, and elongation of bananas in in vitro propagation.Keywords: Agriculture, in vitro propagation, induction, proliferation, elongation, apical meristem, plant growth regulators, cultivars, Isabela, Philippines

scholarly journals Attraction of Yellow Stem Borer (Scirpophaga incertulas Walker) to Different Colored Light-emitting Diodes

2019 ◽  
Vol 23 (2) ◽  
pp. 219
Author(s):  
Yhone Arialistya ◽  
Suputa Suputa ◽  
Y. Andi Trisyono ◽  
Eka Firmansyah
Keyword(s):  
Light Emitting Diodes ◽  
Block Design ◽  
Stem Borer ◽  
Light Sources ◽  
White Led ◽  
Scirpophaga Incertulas ◽  
Light Emitting ◽  
Yellow Stem Borer ◽  
Randomized Block Design ◽  
Normal Green

The yellow rice stem borer (Scirpophaga incertulas Walker) is a major pests on rice. Light traps have been used for monitoring and controlling S. incertulas with white and ultraviolet color light sources. The purpose of this research was to invent trap which uses an effective color that attracts S. incertulas. This research begins by sampling female S. incertulas pre-oviposition from the field then tested in the laboratory. The experiment was designed with factorial randomized block design with light colors and light intencity as factors. Light-emitting diodes used in this research, consisted of normal red, normal yellow, normal green, superbright red, superbright yellow, superbright green, superbright blue, UV, and white. LED white, UV, and superbright blue were attractive colors to S. incertulas  when exposure time was 15 minutes. Effective light intencity to attract S. incertulas was 1000 lux.

scholarly journals Morphogenetic and physiological effects of LED spectra on the apical buds of Ficus carica var. Black Jack

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ankita Rajendra Parab ◽  
Kho Ying Han ◽  
Bee Lynn Chew ◽  
Sreeramanan Subramaniam
Keyword(s):  
Callus Formation ◽  
Light Emitting Diode ◽  
Multiple Shoots ◽  
Cellular Level ◽  
Ficus Carica ◽  
Light Sources ◽  
Light Emitting ◽  
Apical Buds ◽  
Red Led

AbstractThe use of artificial light sources such as light-emitting diodes (LEDs) has become a prerequisite in tissue culture studies to obtain morphogenetic enhancements on in vitro plants. This technology is essential for developmental enhancements in the growing plant cultures due to its light quality and intensity greatly influencing the in vitro growing explants at a cellular level. The current study investigates the effects of different light-emitting diode (LED) spectra on the growth of apical buds of Ficus carica var. Black Jack. Ficus carica, commonly known as figs is rich in vitamins, minerals, and phytochemicals capable of treating microbial infections and gastric, inflammatory, and cardiac disorders. Apical buds of Ficus carica var. Black Jack, presented morphogenetic changes when grown under six different LED spectra. The highest multiple shoots (1.80 per growing explant) and healthy growing cultures were observed under the blue + red LED spectrum. Wound-induced callus formation was observed on apical buds grown under green LED spectrum and discolouration of the growing shoots were observed on the cultures grown under far-red LED spectrum. Multiple shoots obtained from the blue + red LED treatment were rooted using 8 µM indole-3-acetic acid (IAA), and the rooted plantlets were successfully acclimatised. Compared with the other monochromatic LEDs, blue + red proved to be significantly better for producing excellent plant morphogeny. It is apparent that blue and red LED is the most suitable spectra for the healthy development of plants. The findings have confirmed that the combination of blue + red LED can potentially be used for enhancing growth yields of medicinally and commercially important plants.

scholarly journals The Growth and Development of Sweet Basil (Ocimum basilicum) and Bush Basil (Ocimum minimum) Grown under Three Light Regimes in a Controlled Environment

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 743 ◽  
Author(s):  
Aldarkazali ◽  
Rihan ◽  
Carne ◽  
Fuller
Keyword(s):  
Plant Growth ◽  
Radiant Heat ◽  
Oil Yield ◽  
Light Sources ◽  
White Led ◽  
Light Emitting ◽  
Sweet Basil ◽  
Light Regimes ◽  
Led Array ◽  
Red Led

Light is a crucial element for plant growth and production. In areas where natural light is not sufficient for optimal plant growth and production, high pressure sodium (HPS) light sources are widely used. However, HPS lamps are considered not very electrically efficient generating high radiant heat and as a consequence, there has been a lot of interest in replacing HPS lamps with new more efficient lighting sources in the form of light-emitting diodes (LEDs). The effects of three lighting sources (White LED, Blue/Red LED and HPS) on the growth, development and on the essential oil yield and quality of sweet basil and bush basil were investigated. There was a clear advantage to the Blue/Red (452 nm and 632 nm, respectively) LED on virtually all growth and physiological parameters measured for both basil species. The HPS lighting system always performed least effectively in all comparisons. Combining increases in plant yield and increases in oil yield the Blue/Red LED array outperformed the HPS lights by a factor of approximately double, with the white LED being intermediate between these two extremes.

Responses of Favorita Potato Plantlets Cultured in Vitro under Fluorescent and Light-Emitting Diode (LED) Light Sources

2019 ◽  
Vol 96 (4) ◽  
pp. 396-402 ◽  
Author(s):  
Lili Jiang ◽  
Ziquan Wang ◽  
Guanghui Jin ◽  
Dianqiu Lu ◽  
Xuezhan Li
Keyword(s):  
Light Emitting Diode ◽  
Light Sources ◽  
Light Emitting ◽  
Led Light ◽  
Potato Plantlets

scholarly journals Effect of BAP (6-Benzyl Aminopurine) on In Vitro Shoot Growth of Curcumas

2021 ◽  
Vol 4 (1) ◽  
pp. 82-92
Author(s):  
Rustikawati Rustikawati ◽  
Catur Herison ◽  
Entang Inoriah ◽  
Vera Dwisari
Keyword(s):  
Shoot Growth ◽  
Shoot Formation ◽  
Experimental Unit ◽  
Shoot Height ◽  
Weight Percentage ◽  
Randomized Design ◽  
Significant Difference ◽  
Long Time ◽  
Planting Materials

Curcuma sp has been widely investigated for its anti-cancer properties. Conventionally, vegetative propagation needs a long time to produce a large number of planting materials, so that it is necessary to find an alternative approach through in vitro propagation.  The effect of BAP on the in vitro shoot formation of ‘temu putih’ and ‘temu putih’ has been investigated in this study. The experiment was a 4x2 factorial with 5 replications arranged in a completely randomized design.  The first factor was the concentration of BAP i.e. 0, 1.5, 3, and 4.5 ppm.  The second factor was the curcuma species consisting of ‘temu putih’ (Curcuma zedoaria Roch.) and ‘temu mangga’ (Curcuma mangga Val.).  Each experimental unit consisted of 2 in vitro bottles, each of which planted with 1 explant bud.  Analysis of variance was conducted on percentage of live explants, shoot height, number of roots, root length, wet weight, percentage of explants that sprouted, percentage of rooted explants and shoot color.  Mean comparison was performed by the Least Significant Difference (LSD).  The results showed that there was no interaction between BAP concentration and genotype on any variable observed.  The shoot growth of ‘temu putih’ was significantly higher than ‘temu mangga’ in vitro.  The best concentration of BAP for the growth of ‘temu putih’ and ‘temu mangga’ shoots was 1.5 ppm.

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