scholarly journals Screening of jute and kenaf varieties for salinity tolerance

2018 ◽  
Vol 6 (2) ◽  
pp. 214
Author(s):  
Md. Isfatuzzaman Bhuyan ◽  
K M. Mehadi Hassan ◽  
Nowrose Jahan Lipi ◽  
Md Rafiq Uddin ◽  
Md Monirul Islam ◽  
...  
Keyword(s):  
Plant Height ◽  
Salinity Stress ◽  
Salinity Tolerance ◽  
Dry Weight ◽  
Germination Inhibition ◽  
Germination Test ◽  
Salinity Levels ◽  
Total Plant ◽  
Number Of Leaves ◽  
Total Dry Weight

A study was conducted in the Department of Agronomy, Bangladesh Agricultural University (BAU), Mymensingh from April to August 2012 to examine the salinity tolerance of eight jute varieties (CVE-3, C-83, CVL-1, BJC-7370, O-795, O-9897, OM-1, O-72) and two kenaf varieties (HC-95 and HC-2). Initially germination of these varieties were evaluated under six salinity levels viz. 0mM, 20mM, 40mM, 60mM, 80mM, and 100mM NaCl in the seed laboratory of the Department of Agronomy. Afterwards, all the varieties was grown in pots in the net house under four salinity levels viz. 0mM, 25mM, 50mM, and 75mM. The results from the germination study revealed that under control condition (0mM NaCl) all the jute varieties showed germination more than 80% both at 7 and at 14 days after seed sowing, whereas kenaf varieties had germination a little less than 80%. Among the jute varieties, O-72 showed the highest germination (92%), which was statistically similar with those of OM-1(91%), O-795 (90%), and C-83(87%). Salinity stress decreased germination drastically in all of the jute and kenaf varieties. A salinity level of 100mM caused the highest germination inhibition (74.70%) in jute variety CVL-1, which was very close to those of BJC-7370 and O-72. On the other hand, the lowest germination inhibition (51.11%) was recorded in jute variety O-795. The results of the pot trail showed that the plant characters of jute and kenaf varieties were affected significantly by salinity stress. All the varieties produced their respective plant height, number of leaves per plant, and plant dry weight under control condition (no salinity). Among these varieties, CVE-3 produced the highest plant height (145.2cm), and total dry weight (22.55g), whereas O-72 produced the highest number of leaves per plant (24.67). All these plant characters decreased sharply due to salinity stress irrespective of variety. However, the rate of decrease of plant characters occurred differentially in the jute and kenaf varieties. The highest rate of decrease in number of leaves (74.22%) was found from the variety CVE-3, whereas the lowest one was recorded from the variety HC-2 (51.68%). Salinity stress caused the highest decrease in total plant dry weight (73.68%) in the variety CVL-1 and plant height (73.64%) also in the variety CVL-1. On the contrary, the lowest decrease in plant dry weight (50.99%) was found in the variety O-9897 and plant height also in variety O-9897 (50.88%). Based on the results from germination test and pot trail, it can be inferred that jute variety O-9897 appeared to be the most salt tolerant followed by O-795, HC-2, HC-95, CVE-3, O-72, C-83, BJC-7370, OM-1, and CVL-1.    

scholarly journals Toleransi Plasma Nutfah Padi Lokal terhadap Salinitas

2018 ◽  
Vol 23 (1) ◽  
pp. 51 ◽  
Author(s):  
Tintin Suhartini ◽  
Try Zulchi
Keyword(s):  
Plant Height ◽  
Salinity Tolerance ◽  
Negative Correlation ◽  
Dry Weight ◽  
Gene Bank ◽  
Dead Leaf ◽  
Randomized Design ◽  
Highly Sensitive ◽  
Number Of Leaves ◽  
Completely Randomized Design

<p><strong>Abstract</strong></p><p>The research was aimed to screen the local rice germplasms for salinity tolerance. The 104 local rice germplasms were derived of collection of Gene Bank of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD) were tested to salinity. The activities was carried out in greenhouse of ICABIOGRAD. The 15 day old seedlings were planted in pots soil contained NaCl 0.4%. One accession of rice was planted in 2 pots, each pot contained 4 seedlings. Observation of plant symptom was conducted after 1 month planted. The experiment was carried out in a Completely Randomized Design with 2 replications. The results of the analysis showed there were different responsses among rice accesions to the treatment. Salinity caused dry and dead leaves, there were very significant differences between the varieties for plant height, dry weight plant, number of total leaves, and number of green leaves. There were a high and negative correlation of plant height and total number of leaves with percentage of dead leaves. The result showed 21 accesions were tolerant to moderately&amp;#8722;tolerant, while Pokkali as a tolerant check, most tolerant. There were two accessions were tolerant with dead leaf percentage &lt;50%, i.e. Tjempo Brondol (reg. 5800) and Gembira Putih (reg. 20602), whereas Pokkali as resistant check had low dead leaf percentage (16.9%) and 19 accessions were moderately-tolerant with dead leaf percentage &lt;70%, and 82 accesions were sensitive to highly-sensitive.</p><p><strong>Abstrak</strong></p><p>Penelitian ini bertujuan untuk menyaring plasma nutfah padi yang memiliki sifat toleran terhadap salinitas. Sejumlah 104 plasma nutfah padi lokal yang berasal dari koleksi Bank Gen BB Biogen diuji terhadap salinitas. Kegiatan dilakukan di rumah kaca BB Biogen. Bibit padi berumur &amp;plusmn;15 hari ditanam pada pot berisi tanah yang sudah dilumpurkan dengan larutan garam NaCl 0,4% (4.000 ppm). Satu aksesi padi ditanam pada 2 pot, masing masing pot berisi 4 tanaman. Setelah berumur 4 minggu dari tanam dilakukan pengamatan meliputi tinggi tanaman, bobot kering akar, bobot kering tanaman, jumlah daun total, jumlah daun hijau, dan jumlah daun mati. Pengujian menggunakan Rancangan Acak Lengkap dengan 2 ulangan. Hasil analisis varians menunjukkan terdapat perbedaan respons plasma nutfah padi terhadap perlakuan salin yang diberikan. Perbedaan sangat nyata terdapat pada tinggi tanaman, bobot kering tanaman, jumlah daun total, dan jumlah daun hijau. Terdapat korelasi yang tinggi dan negatif pada tinggi tanaman dan jumlah daun total dengan persentase daun mati. Hasil pengujian telah terpilih 21 aksesi plasma nutfah padi toleran hingga agak toleran, sedangkan Pokkali sebagai cek sangat toleran. Dua aksesi yang toleran dengan persentase daun mati &lt;50%, yaitu Tjempo Brondol (reg. 5800) dan Gembira Putih (reg. 20602), Pokkali sebagai varietas kontrol dengan tingkat persentase daun mati 16,9%, sedangkan 19 aksesi termasuk kelompok agak toleran dengan persentase daun mati &lt;70%, dan 82 aksesi termasuk kelompok peka hingga sangat peka.</p>

Comparative salinity tolerance of two legumes, Vigna luteola and Vigna vexillata, from the coast of Trinidad

1994 ◽  
Vol 72 (8) ◽  
pp. 1216-1221 ◽  
Author(s):  
O. T. Okusanya ◽  
O. Oyesiku
Keyword(s):  
Salinity Tolerance ◽  
Sea Water ◽  
Dry Weight ◽  
Sodium Content ◽  
Root Weight ◽  
Growth Responses ◽  
Vigna Vexillata ◽  
Salinity Levels ◽  
Total Plant ◽  
Vigna Luteola

The germination and growth responses of two legumes, Vigna luteola and Vigna vexillata, to different salinity levels were compared in laboratory experiments. Vigna luteola seeds tolerated a higher level of salinity and germinated significantly better at high salinities than corresponding results for V. vexillata. Seedlings of V. luteola exhibited a significant increase in dry weight at 10% sea water followed by a significant decrease at 30% seawater and above. Vigna vexillata seedlings showed a gradual decrease in dry weight with increased salinity. Except at 0% seawater, dry weights of V. luteola seedlings were significantly higher than those of V. vexillata at all salinities tested. The proportion of root weight to total plant dry weight increased with increased salinity in V. luteola; the reverse was the case in V. vexillata. In both species, increased salinity resulted in increased sodium content but decreased potassium and calcium contents. While the sodium content of the shoot of V. vexillata was higher than that of V. luteola, the amount in the roots of V. luteola was higher. The potassium and calcium contents were higher in V. luteola than in V. vexillata and the sodium to potassium ratio values were lower in V. luteola than in V. vexillata. The results are compared with those of other legumes and discussed in relation to the habitats of the species, the characteristics of halophytes, and the uses which these legumes may have in salt-enriched lands. Key words: salinity, tolerance, germination, growth, Vigna.

scholarly journals Adaptation Selection of Plants for Utilization in Phytoremediation of Soil Contaminated by Crude Oil

2020 ◽  
Vol 27 (1) ◽  
pp. 45
Author(s):  
Fadliah Salim ◽  
Yadi Setiadi ◽  
Didy Sopandie ◽  
Mohamad Yani
Keyword(s):  
Crude Oil ◽  
Plant Height ◽  
Contaminated Soil ◽  
Root Length ◽  
Dry Weight ◽  
Shoot Dry Weight ◽  
Shoot Ratio ◽  
Root Shoot Ratio ◽  
Number Of Leaves ◽  
Total Dry Weight

The research on adaptation selection for utilization in phytoremediation of soil contaminated by crude oil using four type plants, such as Helianthus annuus, Paspalum conjugatum, Sorghum bicolor, and Tagetes erecta were conducted. The adaptability of four types of plants  on crude oil contaminated soil at total petroleum hydrocarbon (TPH) level at 0, 3, and 6% were observed and evaluated to their morphological and anatomical responses. Parameters observed were vegetative growth of plants including growth precentage, plant height, number of leaves, root length, root dry weight, shoot dry weight, root/shoot ratio, total dry weight, and stomatal density for 9 weeks cultivation in screen house. The results show that increasing in TPH level caused in significant reductions on morphological of four plants, such as percentage of plant growth, plant height, number of leaves, root length, root dry weight, shoot dry weight, and total dry weight. In contrast, the increasing in TPH level caused to increasing in root/shoot ratio. The four types of plants studied were effective to be used as plants for phytoremediation of petroleum contaminated soil. The plants of P. conjugatum and S. bicolor are recommended as phytoremediators for further studies.

scholarly journals Analysis of the Anticancer Phytochemicals inAndrographis paniculataNees. under Salinity Stress

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Daryush Talei ◽  
Alireza Valdiani ◽  
Mahmood Maziah ◽  
Sreenivasa Rao Sagineedu ◽  
Mohd Said Saad
Keyword(s):  
Salinity Stress ◽  
Morphological Traits ◽  
Dry Weight ◽  
Proline Content ◽  
Tolerance Index ◽  
Maximum Increase ◽  
Physiological Processes ◽  
Salinity Levels ◽  
Total Dry Weight ◽  
Salt Tolerance Index

Salinity causes the adverse effects in all physiological processes of plants. The present study aimed to investigate the potential of salt stress to enhance the accumulation of the anticancer phytochemicals inAndrographis paniculataaccessions. For this purpose, 70-day-old plants were grown in different salinity levels (0.18, 4, 8, 12, and 16 dSm−1) on sand medium. After inducing a period of 30-day salinity stress and before flowering, all plants were harvested and the data on morphological traits, proline content and the three anticancer phytochemicals, including andrographolide (AG), neoandrographolide (NAG), and 14-deoxy-11,12-didehydroandrographolide (DDAG), were measured. The results indicated that salinity had a significant effect on the aforementioned three anticancer phytochemicals. In addition, the salt tolerance index (STI) was significantly decreased, while, except for DDAG, the content of proline, the AG, and NAG was significantly increased (P≤0.01). Furthermore, it was revealed that significant differences among accessions could happen based on the total dry weight, STI, AG, and NAG. Finally, we noticed that the salinity at 12 dSm−1led to the maximum increase in the quantities of AG, NAG, and DDAG. In other words, under salinity stress, the tolerant accessions were capable of accumulating the higher amounts of proline, AG, and NAG than the sensitive accessions.

scholarly journals Assessment of Morpho-Physiological, Biochemical and Antioxidant Responses of Tomato Landraces to Salinity Stress

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 696
Author(s):  
Reem H. Alzahib ◽  
Hussein M. Migdadi ◽  
Abdullah A. Al Ghamdi ◽  
Mona S. Alwahibi ◽  
Abdullah A. Ibrahim ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salinity Stress ◽  
Dry Weight ◽  
Proline Content ◽  
Enzymatic Antioxidants ◽  
Sod Activity ◽  
Carotene Content ◽  
Antioxidants Activity ◽  
Solanum Lycopersicum L ◽  
Salinity Levels

Understanding salt tolerance in tomato (Solanum lycopersicum L.) landraces will facilitate their use in genetic improvement. The study assessed the morpho-physiological variability of Hail tomato landraces in response to different salinity levels at seedling stages and recommended a tomato salt-tolerant landrace for future breeding programs. Three tomato landraces, Hail 548, Hail 747, and Hail 1072 were tested under three salinity levels: 75, 150, and 300 mM NaCl. Salinity stress reduced shoots’ fresh and dry weight by 71% and 72%, and roots were 86.5% and 78.6%, respectively. There was 22% reduced chlorophyll content, carotene content by 18.6%, and anthocyanin by 41.1%. Proline content increased for stressed treatments. The 300 mM NaCl treatment recorded the most proline content increases (67.37 mg/g fresh weight), with a percent increase in proline reaching 61.67% in Hail 747. Superoxide dismutase (SOD) activity decreased by 65% in Hail 548, while it relatively increased in Hail 747 and Hail 1072 treated with 300 mM NaCl. Catalase (CAT) activity was enhanced by salt stress in Hail 548 and recorded 7.6%, increasing at 75 and 5.1% at 300 mM NaCl. It revealed a reduction in malondialdehyde (MDA) at the 300 mM NaCl concentration in both Hail 548 and Hail 1072 landraces. Increasing salt concentrations showed a reduction in transpiration rate of 70.55%, 7.13% in stomatal conductance, and 72.34% in photosynthetic rate. K+/Na+ ratios decreased from 56% for 75 mM NaCl to 85% for 300 mM NaCl treatments in all genotypes. The response to salt stress in landraces involved some modifications in morphology, physiology, and metabolism. The landrace Hail 548 may have better protection against salt stress and observed protection against reactive oxygen species (ROS) by increasing enzymatic “antioxidants” activity under salt stress.

Effects of Temperature and CO2Concentration on the Growth of Cotton (Gossypium hirsutum), Spurred Anoda (Anoda cristata), and Velvetleaf (Abutilon theophrasti)

Weed Science ◽  
1988 ◽  
Vol 36 (6) ◽  
pp. 751-757 ◽  
Author(s):  
David T. Patterson ◽  
Maxine T. Highsmith ◽  
Elizabeth P. Flint
Keyword(s):  
Dry Matter ◽  
Dry Weight ◽  
Net Assimilation Rate ◽  
Assimilation Rate ◽  
Short Term ◽  
Leaf Weight ◽  
Matter Production ◽  
Total Plant ◽  
Net Assimilation ◽  
Total Dry Weight

Cotton, spurred anoda, and velvetleaf were grown in controlled-environment chambers at day/night temperatures of 32/23 or 26/17 C and CO2concentrations of 350 or 700 ppm. After 5 weeks, CO2enrichment to 700 ppm increased dry matter accumulation by 38, 26, and 29% in cotton, spurred anoda, and velvetleaf, respectively, at 26/17 C and by 61, 41, and 29% at 32/23 C. Increases in leaf weight accounted for over 80% of the increase in total plant weight in cotton and spurred anoda in both temperature regimes. Leaf area was not increased by CO2enrichment. The observed increases in dry matter production with CO2enrichment were caused by increased net assimilation rate. In a second experiment, plants were grown at 350 ppm CO2and 29/23 C day/night for 17 days before exposure to 700 ppm CO2at 26/17 C for 1 week. Short-term exposure to high CO2significantly increased net assimilation rate, dry matter production, total dry weight, leaf dry weight, and specific leaf weight in comparison with plants maintained at 350 ppm CO2at 26/17 C. Increases in leaf weight in response to short-term CO2enrichment accounted for 100, 87, and 68% of the observed increase in total plant dry weight of cotton, spurred anoda, and velvetleaf, respectively. Comparisons among the species showed that CO2enrichment decreased the weed/crop ratio for total dry weight, possibly indicating a potential competitive advantage for cotton under elevated CO2, even at suboptimum temperatures.

scholarly journals Tolerance of Basil Genotypes to Salinity

2017 ◽  
Vol 9 (11) ◽  
pp. 283 ◽  
Author(s):  
Renata V. Menezes ◽  
André D. Azevedo Neto ◽  
Hans R. Gheyi ◽  
Alide M. W. Cova ◽  
Hewsley H. B. Silva
Keyword(s):  
Salinity Tolerance ◽  
Dry Matter ◽  
Membrane Integrity ◽  
Dry Mass ◽  
Ocimum Basilicum ◽  
Medicinal Species ◽  
Relative Water ◽  
Salinity Levels ◽  
Number Of Leaves ◽  
Inorganic Solutes

Basil (Ocimum basilicum L.) is a medicinal species of Lamiaceae family, popularly known for its multiple benefits and high levels of volatile compounds. The species is considered to be one of the most essential oil producing plants. Also cultivated in Brazil as a condiment plant in home gardens. The objective of this study was to evaluate the effect of salinity on the growth of basil in nutrient solution of Furlani and to identify variables related to the salinity tolerance in this species. The first assay was performed with variation of five saline levels (0 - control, 20, 40, 60 and 80 mM NaCl). In the second assay six genotypes were evaluated in two salinity levels 0 and 80 mM NaCl. The height, stem diameter, number of leaves, dry mass and inorganic solutes in different organs, photosynthetic pigments, absolute membrane integrity and relative water content were evaluated. All biometric variables in basil were significantly reduced by salinity. Dry matter yield and percentage of membrane integrity were the variables that best discriminated the characteristics of salinity tolerance among the studied basil genotypes. Basil genotypes showed a differentiated tolerance among the genotypes, the ‘Toscano folha de alface’ being considered as the most tolerant and ‘Gennaro de menta’ as the most sensitive, among the species studied.

scholarly journals Effect of Salinity Levels (NaCl) on Yield, Yield Components and Quality Content of Sesame (Sesamum Indicum L.) Cultivars

2016 ◽  
Vol 5 (2) ◽  
pp. 104
Author(s):  
Helale Bahrami ◽  
Amir Ostadi Jafari ◽  
Jamshid Razmjoo
Keyword(s):  
Plant Height ◽  
Yield Components ◽  
Seed Oil ◽  
Tap Water ◽  
Dry Weight ◽  
Salinity Level ◽  
Shoot Dry Weight ◽  
Weight Yield ◽  
Yield And Yield Components ◽  
Salinity Levels

<p class="emsd-body"><span lang="EN-GB">Seeds of ten sesame cultivars (Karaj, Darab, Safiabad, Jiroft, Borazjan, Yellow-white, Felestin, Ultan, Isfahan and Abpakhsh) were sown into soil filled pots in 2008 and 2009. Pots were watered with six levels of salts (0.0038 (tap water as control), 4.89, 8.61, 10.5, 14.54, 17.74 ds.m<sup>-1</sup> NaCl) until full maturity. Plant height, root and shoot dry weight, yield and yield components, seed oil and protein contents of cultivars were measured. Increasing salinity caused significant reduction in plant height, root and shoot dry weight, yield and yield components, seed oil and protein contents of all cultivars. However, there were significant differences among the cultivars for measured traits for each salinity level. Based on seed oil yield data, Safiabad and Kraj at 0.0038ds.m<sup>-1</sup>, Safiabad and Ultan at 4.89ds.m<sup>-1</sup>, Ultan, Safiabad and Darab at 8.61 salinity levels were the superior cultivars. High variability in tolerance to salinity among the tested sesame cultivars suggests that selection of more salt tolerant cultivars for planting or breeding purposes is possible.</span></p><p class="emsd-body"><span lang="EN-GB">Highlights</span></p><p class="emsd-body"><span lang="EN-GB">Effects of contrasting salinity levels (0.0038 (tap water as control), 4.89, 8.61, 10.5, 14.54, 17.74 ds.m<sup>-1</sup>NaCl) on sesame cultivars were tested. Salinity reduced plant growth and yield and seed oil and protein contents. However, there were significant differences among the cultivars for measured traits for each salinity level. </span></p>

scholarly journals Effect of Planting Date and Gibberellic Acid on the Growth and Yield of Garlic

2010 ◽  
pp. 132-140 ◽  
Author(s):  
MA Islam ◽  
MH Reza ◽  
SMAHM Kamal ◽  
MA Wazed ◽  
KM Islam
Keyword(s):  
Gibberellic Acid ◽  
Plant Height ◽  
Dry Matter ◽  
Dry Weight ◽  
Planting Date ◽  
Growth And Yield ◽  
Planting Time ◽  
Local Cultivar ◽  
Number Of Leaves ◽  
Bulb Yield

An experiment was conducted with a local cultivar of garlic to study the effects of planting date and gibberellic acid on the growth and yield of garlic at the field laboratory of the Department of Crop Botany, Bangladesh Agricultural University, Mymensingh during November 2001 to April 2002. Early planting influenced the plant height, leaf number, bulb diameter and total dry matter. With the delay in planting time starting from November 7, the yield was chronologically reduced in later plantings. Significantly the highest bulb yield (2.67 t/ha) was recorded when planting was done on November 7 and lowest yield (0.92 t/ha) was obtained from December 22 planted crop. Bulb yield was higher in control plants than those of GA3 treated plants. The interaction effects of planting date and different concentrations of GA3 differed significantly in respect of plant height, number of leaves, bulb diameter and dry weight of roots, leaves and bulbs and yield of garlic.

scholarly journals MODIFICAÇÕES NO MEIO DE CULTURA, FOTOPERÍODO E TEMPO DE CULTIVO AFETAM O ALONGAMENTO E ENRAIZAMENTO IN VITRO DE BANANEIRA CV. PACOVAN

Nativa ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 27
Author(s):  
Marcos Vinícius Marques Pinheiro ◽  
Ana Cristina Portugal Pinto De Carvalho ◽  
Fabrina Bolzan Martins
Keyword(s):  
Plant Height ◽  
Culture Medium ◽  
Culture Media ◽  
Dry Weight ◽  
Salt Mixture ◽  
Musa Spp ◽  
Fresh Biomass ◽  
Shoot Dry Weight ◽  
Number Of Leaves

No intuito de elevar as taxas de sobrevivência durante a etapa de aclimatização e posterior plantio a campo, avaliou-se o enraizamento in vitro de bananeira cv. Pacovan, em diferentes concentrações de sais MS e de sacarose. Utilizou-se DIC, esquema fatorial (6x2x3), com seis meios de cultura [sendo três concentrações de nutrientes do meio MS (100%; 50% de macronutrientes; e 50% dos sais macro e micronutrientes), e duas concentrações de sacarose (1,5/3,0%)], dois fotoperíodos (12/16 h) e três tempos de cultivo (21, 28 ou 35 dias) e seis repetições/tratamento. Analisaram-se: altura da planta, número de folhas/planta, massas frescas e secas das partes aérea e radicular. Para altura da planta, massa fresca da parte aérea e radicular, o meio MS 50% dos sais + sacarose (1,5%) com fotoperíodo de 16 h e tempo de cultivo de 35 dias foi satisfatório. Para massa seca da parte aérea foi MS 50% de sais + sacarose (3%), e para massa seca da parte radicular, MS 100% + sacarose (3%) (em 12hs/28 dias e 16hs/21 dias). Para o alongamento/enraizamento in vitro da bananeira cv. Pacovan sugere-se MS 50% de sais (macro e micronutrientes), redução ou manutenção de sacarose (1,5 ou 3%) em 16h/35 dias de cultivo.Palavra-chave: Musa spp., propagação in vitro, sistema radicular. CHANGES IN CULTURE MEDIUM, PHOTOPERIOD AND TIME OF CULTIVATION AFFECT THE IN VITRO ELONGATION AND ROOTING OF BANANA CV. PACOVAN ABSTRACT:In order to achieve high rates of survival during the acclimatization and later planting in the field, was evaluated the in vitro of banana cv. Pacovan plants under different concentrations of sucrose and MS basal salt mixture. The experiment was assembled in a DIC, in 6x2x3, six different culture media [three different MS salt mixture concentrations (100%; 50% of macronutrients; and 50% of macro/micronutrients) and two sucrose concentrations (1.5/3%)], two photoperiods (12/16 hours) and three cultivation times (21, 28 or 35 days). Each treatment was composed by 6 replicates. Plant height, number of leaves/plant, fresh and dry weight of roots and shoots, were analyzed. Satisfactory results for plant height and shoot and root fresh biomass were observed in MS with macro/micronutrients (50%) + sucrose (3%), 16 hours/35 days. The highest values of shoot dry weight were observed in MS with macro/micronutrients (50%) + sucrose (3%); the highest root dry weight was achieved with MS 100% + sucrose (3%) (12hs/28 and 16hs/21 days). The suggested medium for the in vitro elongation and rooting stage of banana cv. Pacovan is the MS with 50% of salts (macro and micronutrients), reduction or maintenance of sucrose (1.5 or 3%) in 16h/35 days of cultivation.Keywords: Musa spp., in vitro propagation, root system. DOI:

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